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Uhlig, Christiane; Loose, Brice (2017): Methane oxidation in Arctic seawater, Utqiagvik, Alaska. PANGAEA,, Supplement to: Uhlig, C; Loose, B (2017): Using stable isotopes and gas concentrations for independent constraints on microbial methane oxidation at Arctic Ocean temperatures. Limnology and Oceanography-Methods, 15(8), 737-751,

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Arctic seawater was collected at two sites close to Utqiagvik, North Slope, Alaska on 7, 9 and 15 April 2016 in the Beaufort Sea. Site "Elson Lagoon" (EL) was located North of Utqiagvik (2016-04-07, 71.334° N, -156.363° W), covered with 1.5 m thick sea ice and shallow with approximately 1.5 m depth. Water was collected from a depth of 1.5 m in a narrow layer of water between the sea ice and the sediment. Site "Ice Mass Balance Buoy" (IMB) was located 1 km offshore of Utqiagvik (2016-04-07, 71.373° N, -156.548° W), and (2016-04-09, 71.372° N, -156.540° W). This site was characterized by 1 m thick fast ice cover and a water depth of approximately 7 m. Water was collected at five depths from 1 to 6.5 m.
Seawater was additionally sampled in Narragansett Bay, Rhode Island, from the pier of the Graduate School of Oceanography, University of Rhode Island (41.492° N, -71.419° W) on 15 July 2016 to test our method for applicability under different environmental conditions. Sampling depth was 2-3 m at a water depth of approx. 6 m.
Seawater was amended with different methane concentrations to determine methane oxidation rates in a headspace approach. Methane concentration and isotope ratios were determined with a Picarro G2201-i cavity ring-down spectrometer (Picarro, Santa Clara, California, USA) coupled to a Small Sample Isotope Module (SSIM) (Picarro, Santa Clara, California, USA). Several treatments were amended with sodium hydroxide (NaOH) to serve as killed controls or with 2-bromoethanesulphonate (BES) an inhibitor for methane production.
Related to:
Uhlig, Christiane; Kirkpatrick, John B; d'Hondt, Steven L; Loose, Brice (2018): Methane-oxidizing seawater microbial communities from an Arctic shelf. Biogeosciences, 15, 3311-3329,
Median Latitude: 65.388600 * Median Longitude: -139.482000 * South-bound Latitude: 41.492000 * West-bound Longitude: -156.548000 * North-bound Latitude: 71.373000 * East-bound Longitude: -71.419000
Date/Time Start: 2016-04-07T00:00:00 * Date/Time End: 2016-07-15T00:00:00
Minimum DEPTH, water: 1.0 m * Maximum DEPTH, water: 6.5 m
Elson_Lagoon * Latitude: 71.334000 * Longitude: -156.363000 * Date/Time: 2016-04-07T00:00:00 * Method/Device: Multiple investigations (MULT)
Narragansett_Bay * Latitude: 41.492000 * Longitude: -71.419000 * Date/Time: 2016-07-15T00:00:00 * Elevation: -6.0 m * Method/Device: Multiple investigations (MULT)
Utqiagvik_IMB-1 * Latitude: 71.373000 * Longitude: -156.548000 * Date/Time: 2016-04-07T00:00:00 * Elevation: -7.0 m * Method/Device: Multiple investigations (MULT)
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Event labelEventUhlig, Christiane
2Date/Time of eventDate/TimeUhlig, Christiane
3Latitude of eventLatitudeUhlig, Christiane
4Longitude of eventLongitudeUhlig, Christiane
5DEPTH, waterDepth watermUhlig, ChristianeGeocode
6Depth, top/minDepth topmUhlig, Christiane
7Depth, bottom/maxDepth botmUhlig, Christiane
8Sample IDSample IDUhlig, Christiane
9TreatmentTreatUhlig, Christiane
10Sample amountN#Uhlig, Christiane
11Duration, number of daysDurationdaysUhlig, Christiane
12Duration, number of days, standard deviationDuration std dev±Uhlig, Christiane
13MethaneCH4nmol/lUhlig, ChristianeCavity ring-down spectrometer G2201-i, coupled to a small sample isotope modulemean
14Methane, standard deviationCH4 std dev±Uhlig, ChristianeCavity ring-down spectrometer G2201-i, coupled to a small sample isotope module
15Methane, natural logarithmln(CH4)nmolUhlig, ChristianeCalculatedmean
16Methane, natural logarithm, standard deviationln(CH4) std dev±Uhlig, ChristianeCalculated
17δ13C, methaneδ13C CH4‰ PDBUhlig, ChristianeCavity ring-down spectrometer G2201-i, coupled to a small sample isotope modulemean, VPDB
18δ13C, methane, standard deviationδ13C CH4 std dev±Uhlig, ChristianeCavity ring-down spectrometer G2201-i, coupled to a small sample isotope module
19Y-axis high meanY-axis high meanUhlig, ChristianeCalculated, see reference(s)
20Y-axis high, standard deviationY-axis high std dev±Uhlig, ChristianeCalculated, see reference(s)
21Y-axis low meanY-axis low meanUhlig, ChristianeCalculated, see reference(s)
22Y-axis low, standard deviationY-axis low std dev±Uhlig, ChristianeCalculated, see reference(s)
4152 data points

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