/* DATA DESCRIPTION: Citation: Hoffmann, Katy; Bienhold, Christina; Buttigieg, Pier Luigi; Laso-Pérez, Rafael; Fadeev, Eduard; Rapp, Josephine Z; Boetius, Antje; Offre, Pierre (2017): Abundance of Prokaryotes in deep-sea sediments analyzed by CARD-FISH. PANGAEA, https://doi.org/10.1594/PANGAEA.874860, In supplement to: Hoffmann, Katy; Bienhold, Christina; Buttigieg, Pier Luigi; Knittel, Katrin; Laso-Pérez, Rafael; Rapp, Josephine Z; Boetius, Antje; Offre, Pierre (2020): Diversity and metabolism of Woeseiales bacteria, global members of marine sediment communities. The ISME Journal, 14(4), 1042-1056, https://doi.org/10.1038/s41396-020-0588-4 Abstract: We fixed 0.5 g aliquots of sediment with a 4% formaldehyde solution for 2-4 h, washed the fixed sediments three times with 1x phosphate-buffered saline (PBS), before storing them in 50% ethanol/PBS at -20°C. For water samples, we fixed 10 ml (for samples from the deep chlorophyll maximum and 100 m water depth) and 30 ml (for meso- and bathypelagic samples) with formaldehyde to a final concentration of 2-4% for 2-4 h, then filtered over a 0.22 µm polycarbonate filter, and stored samples at -20°C. We performed total cell counts as described by Schauer et al. (2011, doi:10.1111/j.1462-2920.2011.02530.x) using the nucleic acid dye 4'-6-diamidino-2-phenylindole (DAPI). A minimum of 1,000 cells in 20 independent grids were counted using a Zeiss Axio Imager M1 epifluorescence microscope equipped with a 100x/1.25 oil plan-apochromat objective. We used Catalyzed Reporter Deposition-Fluorescence In Situ Hybridization (CARD-FISH) according to Ishii et al. (2004) to count Gammaproteobacteria and JTB255 cells. We used the GAM42a oligonucleotide probe and the BET42a competitor probe to target members of the Gammaproteobacteria (Manz et al., 1992, doi:10.1016/S0723-2020(11)80121-9). We designed the JTB819a and JTB897 probes to target 16S rRNA gene sequences assigned to JTB255 in SILVA release 128 and the cJTB897 competitor probe to target all non-JTB255 16S sequences in SILVA release 128 that have a single mismatch to JTB819a and JTB897 probes. We obtained total gammaproteobacterial and JTB255 cell counts from duplicate filters derived from each sampling site. Related to: Ruff, S Emil; Felden, Janine; Gruber-Vodicka, Harald R; Marcon, Yann; Knittel, Katrin; Ramette, Alban; Boetius, Antje (2014): Metagenom sequences of Archaea and Bacteria from sediment samples of the Haakon Mosby mud volcano. European Nucleotide Archive (ENA), insdc:PRJNA248084 Schauer, Regina; Røy, Hans; Augustin, Nico; Gennerich, Hans-Hermann; Peters, Marc; Wenzhöfer, Frank; Amann, Rudolf; Meyerdierks, Anke (2011): Bacterial sulfur cycling shapes microbial communities in surface sediments of an ultramafic hydrothermal vent field. Environmental Microbiology, 13(10), 2633-2648, https://doi.org/10.1111/j.1462-2920.2011.02530.x Sokoll, Sarah; Holtappels, Moritz; Lam, Phyllis; Collins, Gavin; Schlüter, Michael; Lavik, Gaute; Kuypers, Marcel MM (2012): Benthic nitrogen loss in the Arabian Sea off Pakistan. Frontiers in Microbiology, 3, https://doi.org/10.3389/fmicb.2012.00395 Thiele, Stefan; Fuchs, Bernhard M; Amann, Rudolf; Iversen, Morten Hvitfeldt; Wommack, K Eric (2015): Colonization in the Photic Zone and Subsequent Changes during Sinking Determine Bacterial Community Composition in Marine Snow. Applied and Environmental Microbiology, 81(4), 1463-1471, https://doi.org/10.1128/AEM.02570-14 Project(s): Assessment of bacterial life and matter cycling in deep-sea surface sediments (ABYSS) (URI: https://www.helmholtz.de/forschung/helmholtz-international/europaeische-projekte/archiv-fp7/ideen/erc-advanced-grants/abyss/) Funding: Seventh Framework Programme (FP7), grant/award no. 294757: Assessment of bacterial life and matter cycling in deep-sea surface sediments (URI: https://cordis.europa.eu/project/id/294757) Coverage: MEDIAN LATITUDE: 22.542717 * MEDIAN LONGITUDE: -15.804435 * SOUTH-BOUND LATITUDE: -53.808830 * WEST-BOUND LONGITUDE: -104.266667 * NORTH-BOUND LATITUDE: 87.933380 * EAST-BOUND LONGITUDE: 63.166680 DATE/TIME START: 2007-01-29T13:17:24 * DATE/TIME END: 2015-09-15T02:36:11 MINIMUM DEPTH, sediment/rock: 0.005 m * MAXIMUM DEPTH, sediment/rock: 0.110 m Event(s): AT26-23-05 * LATITUDE: 9.900000 * LONGITUDE: -104.266667 * ELEVATION: -2600.0 m * METHOD/DEVICE: Piston corer (PC) AT26-23-12 * LATITUDE: 9.883333 * LONGITUDE: -104.233333 * ELEVATION: -2800.0 m * METHOD/DEVICE: Piston corer (PC) GeoB12202-1 (M74/2_962-1) * LATITUDE START: 24.700000 * LONGITUDE START: 63.166660 * LATITUDE END: 24.700000 * LONGITUDE END: 63.166680 * DATE/TIME START: 2007-10-09T02:15:00 * DATE/TIME END: 2007-10-09T03:50:00 * ELEVATION: -1527.0 m * CAMPAIGN: M74/2 (URI: https://doi.org/10.2312/cr_m74) * BASIS: Meteor (1986) (URI: https://en.wikipedia.org/wiki/RV_Meteor_(1986)) * METHOD/DEVICE: MultiCorer (MUC) GeoB12202-2 (M74/2_962-2) * LATITUDE START: 24.700000 * LONGITUDE START: 63.166660 * LATITUDE END: 24.700000 * LONGITUDE END: 63.166680 * DATE/TIME START: 2007-10-09T03:50:00 * DATE/TIME END: 2007-10-09T05:18:00 * ELEVATION: -1526.0 m * CAMPAIGN: M74/2 (URI: https://doi.org/10.2312/cr_m74) * BASIS: Meteor (1986) (URI: https://en.wikipedia.org/wiki/RV_Meteor_(1986)) * METHOD/DEVICE: MultiCorer (MUC) MSM04/3_251-ROV (J2-255) * LATITUDE START: 14.667167 * LONGITUDE START: -45.000548 * LATITUDE END: 14.752737 * LONGITUDE END: -44.979155 * DATE/TIME START: 2007-01-29T13:17:24 * DATE/TIME END: 2007-01-29T21:34:20 * ELEVATION START: -3021.3 m * ELEVATION END: -3022.6 m * LOCATION: tropical/subtropical North Atlantic * CAMPAIGN: MSM04/3 (HYDROMAR-III) (URI: https://doi.org/10.2312/cr_msm04) * BASIS: Maria S. Merian (URI: https://en.wikipedia.org/wiki/RV_Maria_S._Merian) * METHOD/DEVICE: Remote operated vehicle (ROV) * COMMENT: Irina II, OBT site; Irina II T-logger field, mussel/T/fluid sampling, recovery new OPB MSM04/3_259-ROV (J2-258) * LATITUDE START: 14.752225 * LONGITUDE START: -44.980483 * LATITUDE END: 14.753501 * LONGITUDE END: -44.979764 * DATE/TIME START: 2007-02-01T13:20:46 * DATE/TIME END: 2007-02-02T10:08:15 * ELEVATION START: -3032.3 m * ELEVATION END: -3036.5 m * LOCATION: tropical/subtropical North Atlantic * CAMPAIGN: MSM04/3 (HYDROMAR-III) (URI: https://doi.org/10.2312/cr_msm04) * BASIS: Maria S. Merian (URI: https://en.wikipedia.org/wiki/RV_Maria_S._Merian) * METHOD/DEVICE: Remote operated vehicle (ROV) * COMMENT: Irina II, Quest; Chamber, pick up Irina II loggers, recover new OBT MSM04/3_271-ROV (J2-261) * LATITUDE START: 14.752613 * LONGITUDE START: -44.977569 * LATITUDE END: 14.752847 * LONGITUDE END: -44.979596 * DATE/TIME START: 2007-02-05T13:08:51 * DATE/TIME END: 2007-02-05T22:02:22 * ELEVATION START: -3042.5 m * ELEVATION END: -3045.9 m * LOCATION: tropical/subtropical North Atlantic * CAMPAIGN: MSM04/3 (HYDROMAR-III) (URI: https://doi.org/10.2312/cr_msm04) * BASIS: Maria S. Merian (URI: https://en.wikipedia.org/wiki/RV_Maria_S._Merian) * METHOD/DEVICE: Remote operated vehicle (ROV) * COMMENT: Quest PS73/127-7 * LATITUDE: -47.992300 * LONGITUDE: -16.004000 * DATE/TIME: 2009-01-31T03:51:00 * ELEVATION: -4077.0 m * LOCATION: South Atlantic Ocean * CAMPAIGN: ANT-XXV/3 (PS73 LOHAFEX) (URI: https://doi.org/10.2312/BzPM_0613_2010) * BASIS: Polarstern (URI: https://doi.org/10.17815/jlsrf-3-163) * METHOD/DEVICE: MultiCorer (MUC) PS79/086-28 * LATITUDE: -51.979000 * LONGITUDE: -12.035170 * DATE/TIME: 2012-02-01T18:59:00 * LOCATION: South Atlantic Ocean * CAMPAIGN: ANT-XXVIII/3 (PS79) (URI: https://doi.org/10.2312/BzPM_0661_2013) * BASIS: Polarstern (URI: https://doi.org/10.17815/jlsrf-3-163) * METHOD/DEVICE: MultiCorer (MUC) PS79/141-9 * LATITUDE: -51.267170 * LONGITUDE: -12.617670 * DATE/TIME: 2012-02-18T22:58:00 * LOCATION: South Atlantic Ocean * CAMPAIGN: ANT-XXVIII/3 (PS79) (URI: https://doi.org/10.2312/BzPM_0661_2013) * BASIS: Polarstern (URI: https://doi.org/10.17815/jlsrf-3-163) * METHOD/DEVICE: MultiCorer (MUC) PS79/177-3 * LATITUDE: -53.808830 * LONGITUDE: -52.355170 * DATE/TIME: 2012-03-07T13:22:00 * ELEVATION: -339.0 m * LOCATION: South Atlantic Ocean * CAMPAIGN: ANT-XXVIII/3 (PS79) (URI: https://doi.org/10.2312/BzPM_0661_2013) * BASIS: Polarstern (URI: https://doi.org/10.17815/jlsrf-3-163) * METHOD/DEVICE: MultiCorer (MUC) PS80/225-1 * LATITUDE: 84.047320 * LONGITUDE: 31.192630 * DATE/TIME: 2012-08-09T10:35:00 * ELEVATION: -4019.2 m * LOCATION: Arctic Ocean * CAMPAIGN: ARK-XXVII/3 (PS80 IceArc) (URI: https://doi.org/10.2312/BzPM_0663_2013) * BASIS: Polarstern (URI: https://doi.org/10.17815/jlsrf-3-163) * METHOD/DEVICE: Multicorer with television (TVMUC) * COMMENT: SL=4013m; Position sensor: USBL Ixsea Posidonia PS80/350-1 * LATITUDE: 87.933380 * LONGITUDE: 61.169580 * DATE/TIME: 2012-09-18T11:25:00 * ELEVATION: -4380.8 m * LOCATION: Arctic Ocean * CAMPAIGN: ARK-XXVII/3 (PS80 IceArc) (URI: https://doi.org/10.2312/BzPM_0663_2013) * BASIS: Polarstern (URI: https://doi.org/10.17815/jlsrf-3-163) * METHOD/DEVICE: Multicorer with television (TVMUC) * COMMENT: abgesetzt, SL=4363m; Position sensor: USBL Ixsea Posidonia PS81/606-1 * LATITUDE: -51.429830 * LONGITUDE: 14.222330 * DATE/TIME: 2013-11-16T17:51:00 * ELEVATION: -4469.0 m * LOCATION: South Atlantic Ocean * CAMPAIGN: ANT-XXIX/8 (PS81) (URI: https://doi.org/10.2312/BzPM_0672_2014) * BASIS: Polarstern (URI: https://doi.org/10.17815/jlsrf-3-163) * METHOD/DEVICE: Multicorer with television (TVMUC) PS85/436-1 (EGI) * LATITUDE: 78.973330 * LONGITUDE: -5.290500 * DATE/TIME: 2014-06-17T14:30:00 * ELEVATION: -1056.3 m * LOCATION: North Greenland Sea * CAMPAIGN: PS85 (ARK-XXVIII/2) (URI: https://doi.org/10.2312/BzPM_0687_2015) * BASIS: Polarstern (URI: https://doi.org/10.17815/jlsrf-3-163) * METHOD/DEVICE: Multicorer with television (TVMUC) PS85/454-3 * LATITUDE: 78.505670 * LONGITUDE: -2.817670 * DATE/TIME: 2014-06-20T17:59:00 * ELEVATION: -2557.7 m * LOCATION: North Greenland Sea * CAMPAIGN: PS85 (ARK-XXVIII/2) (URI: https://doi.org/10.2312/BzPM_0687_2015) * BASIS: Polarstern (URI: https://doi.org/10.17815/jlsrf-3-163) * METHOD/DEVICE: Multicorer with television (TVMUC) PS85/460-4 (HGIV) * LATITUDE: 79.065170 * LONGITUDE: 4.183000 * DATE/TIME: 2014-06-22T18:47:00 * ELEVATION: -2402.6 m * LOCATION: North Greenland Sea * CAMPAIGN: PS85 (ARK-XXVIII/2) (URI: https://doi.org/10.2312/BzPM_0687_2015) * BASIS: Polarstern (URI: https://doi.org/10.17815/jlsrf-3-163) * METHOD/DEVICE: Multicorer with television (TVMUC) PS85/464-1 (HGVI) * LATITUDE: 79.059170 * LONGITUDE: 3.572830 * DATE/TIME: 2014-06-23T16:31:00 * ELEVATION: -3531.0 m * LOCATION: North Greenland Sea * CAMPAIGN: PS85 (ARK-XXVIII/2) (URI: https://doi.org/10.2312/BzPM_0687_2015) * BASIS: Polarstern (URI: https://doi.org/10.17815/jlsrf-3-163) * METHOD/DEVICE: Multicorer with television (TVMUC) PS85/465-4 (HGIX) * LATITUDE: 79.158330 * LONGITUDE: 2.882830 * DATE/TIME: 2014-06-24T02:48:00 * ELEVATION: -5524.6 m * LOCATION: North Greenland Sea * CAMPAIGN: PS85 (ARK-XXVIII/2) (URI: https://doi.org/10.2312/BzPM_0687_2015) * BASIS: Polarstern (URI: https://doi.org/10.17815/jlsrf-3-163) * METHOD/DEVICE: Multicorer with television (TVMUC) PS85/470-3 (HGI) * LATITUDE: 79.133500 * LONGITUDE: 6.106500 * DATE/TIME: 2014-06-24T23:34:00 * ELEVATION: -1244.2 m * LOCATION: North Greenland Sea * CAMPAIGN: PS85 (ARK-XXVIII/2) (URI: https://doi.org/10.2312/BzPM_0687_2015) * BASIS: Polarstern (URI: https://doi.org/10.17815/jlsrf-3-163) * METHOD/DEVICE: Multicorer with television (TVMUC) PS93/067-2 * LATITUDE: 78.628000 * LONGITUDE: 10.583830 * DATE/TIME: 2015-08-06T17:23:00 * ELEVATION: -66.9 m * LOCATION: North Greenland Sea * CAMPAIGN: PS93.2 (ARK-XXIX/2.2) (URI: https://doi.org/10.2312/BzPM_0693_2015) * BASIS: Polarstern (URI: https://doi.org/10.17815/jlsrf-3-163) * METHOD/DEVICE: Plankton net (PLA) SO242/2_147-148 * LATITUDE: -7.100650 * LONGITUDE: -88.414000 * DATE/TIME: 2015-09-02T00:00:00 * ELEVATION: -4198.0 m * LOCATION: South Pacific Ocean, Peru Basin * CAMPAIGN: SO242/2 (JPI-OCEANS) (URI: https://doi.org/10.3289/GEOMAR_REP_NS_27_2015) * BASIS: Sonne_2 (URI: https://uol.de/icbm/forschungsplattformen-schiffe/sonne) * METHOD/DEVICE: MultiCorer (MUC) * COMMENT: DEA.W.undisturbed SO242/2_194-1 * LATITUDE: -7.076117 * LONGITUDE: -88.526600 * DATE/TIME: 2015-09-15T02:36:11 * ELEVATION: -4078.9 m * LOCATION: South Pacific Ocean, Peru Basin * CAMPAIGN: SO242/2 (JPI-OCEANS) (URI: https://doi.org/10.3289/GEOMAR_REP_NS_27_2015) * BASIS: Sonne_2 (URI: https://uol.de/icbm/forschungsplattformen-schiffe/sonne) * METHOD/DEVICE: MultiCorer (MUC) * COMMENT: Action: max depth/on ground; Position sensor: USBL; Comment: max. Cable Length: 4166m SO242/2_198_MUC * LATITUDE: -7.125610 * LONGITUDE: -88.450930 * DATE/TIME: 2015-09-03T00:00:00 * LOCATION: South Pacific Ocean, Peru Basin * CAMPAIGN: SO242/2 (JPI-OCEANS) (URI: https://doi.org/10.3289/GEOMAR_REP_NS_27_2015) * BASIS: Sonne_2 (URI: https://uol.de/icbm/forschungsplattformen-schiffe/sonne) * METHOD/DEVICE: MultiCorer (MUC) * COMMENT: Polymetallic nodule Parameter(s): Event label (Event) * PI: Hoffmann, Katy (https://orcid.org/0000-0002-7714-4219, khoffman@mpi-bremen.de) Latitude of event (Latitude) * PI: Hoffmann, Katy (https://orcid.org/0000-0002-7714-4219, khoffman@mpi-bremen.de) Longitude of event (Longitude) * PI: Hoffmann, Katy (https://orcid.org/0000-0002-7714-4219, khoffman@mpi-bremen.de) Date/Time of event (Date/Time) * PI: Hoffmann, Katy (https://orcid.org/0000-0002-7714-4219, khoffman@mpi-bremen.de) Elevation of event [m] (Elevation) * PI: Hoffmann, Katy (https://orcid.org/0000-0002-7714-4219, khoffman@mpi-bremen.de) Sample ID (Sample ID) * PI: Hoffmann, Katy (https://orcid.org/0000-0002-7714-4219, khoffman@mpi-bremen.de) Depth, top/min [m] (Depth top) * PI: Hoffmann, Katy (https://orcid.org/0000-0002-7714-4219, khoffman@mpi-bremen.de) Depth, bottom/max [m] (Depth bot) * PI: Hoffmann, Katy (https://orcid.org/0000-0002-7714-4219, khoffman@mpi-bremen.de) DEPTH, sediment/rock [m] (Depth sed) * GEOCODE * PI: Hoffmann, Katy (https://orcid.org/0000-0002-7714-4219, khoffman@mpi-bremen.de) Environmental feature (Env feature) * PI: Hoffmann, Katy (https://orcid.org/0000-0002-7714-4219, khoffman@mpi-bremen.de) * COMMENT: biome Environmental feature (Env feature) * PI: Hoffmann, Katy (https://orcid.org/0000-0002-7714-4219, khoffman@mpi-bremen.de) * COMMENT: material Environmental feature (Env feature) * PI: Hoffmann, Katy (https://orcid.org/0000-0002-7714-4219, khoffman@mpi-bremen.de) * COMMENT: feature Sample type (Samp type) * PI: Hoffmann, Katy (https://orcid.org/0000-0002-7714-4219, khoffman@mpi-bremen.de) Analytical method (Method) * PI: Hoffmann, Katy (https://orcid.org/0000-0002-7714-4219, khoffman@mpi-bremen.de) Reference/source (Reference) * PI: Hoffmann, Katy (https://orcid.org/0000-0002-7714-4219, khoffman@mpi-bremen.de) Comment (Comment) * PI: Hoffmann, Katy (https://orcid.org/0000-0002-7714-4219, khoffman@mpi-bremen.de) Prokaryotes [#/ml] (Prokaryotes) * PI: Hoffmann, Katy (https://orcid.org/0000-0002-7714-4219, khoffman@mpi-bremen.de) * METHOD/DEVICE: Epifluorescence microscopy after DAPI staining (URI: hdl:10013/epic.27912.d001) * COMMENT: minimum Prokaryotes [#/ml] (Prokaryotes) * PI: Hoffmann, Katy (https://orcid.org/0000-0002-7714-4219, khoffman@mpi-bremen.de) * METHOD/DEVICE: Epifluorescence microscopy after DAPI staining (URI: hdl:10013/epic.27912.d001) * COMMENT: maximum Gammaproteobacteria, cells [10**3 #/ml] (Gammaproteob) * PI: Hoffmann, Katy (https://orcid.org/0000-0002-7714-4219, khoffman@mpi-bremen.de) * METHOD/DEVICE: Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH) (URI: https://doi.org/10.1128/AEM.68.6.3094-3101.2002) * COMMENT: minimum Gammaproteobacteria, cells [10**3 #/ml] (Gammaproteob) * PI: Hoffmann, Katy (https://orcid.org/0000-0002-7714-4219, khoffman@mpi-bremen.de) * METHOD/DEVICE: Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH) (URI: https://doi.org/10.1128/AEM.68.6.3094-3101.2002) * COMMENT: maximum Gammaproteobacteria [%] (Gammaproteobacteria) * PI: Hoffmann, Katy (https://orcid.org/0000-0002-7714-4219, khoffman@mpi-bremen.de) * METHOD/DEVICE: Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH) (URI: https://doi.org/10.1128/AEM.68.6.3094-3101.2002) * COMMENT: minimum Gammaproteobacteria [%] (Gammaproteobacteria) * PI: Hoffmann, Katy (https://orcid.org/0000-0002-7714-4219, khoffman@mpi-bremen.de) * METHOD/DEVICE: Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH) (URI: https://doi.org/10.1128/AEM.68.6.3094-3101.2002) * COMMENT: maximum Japan Trench Bacteria clone 255 marine benthic group [#/ml] (JTB255) * PI: Hoffmann, Katy (https://orcid.org/0000-0002-7714-4219, khoffman@mpi-bremen.de) * METHOD/DEVICE: Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH) (URI: https://doi.org/10.1128/AEM.68.6.3094-3101.2002) * COMMENT: minimum Japan Trench Bacteria clone 255 marine benthic group [#/ml] (JTB255) * PI: Hoffmann, Katy (https://orcid.org/0000-0002-7714-4219, khoffman@mpi-bremen.de) * METHOD/DEVICE: Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH) (URI: https://doi.org/10.1128/AEM.68.6.3094-3101.2002) * COMMENT: maximum Japan Trench Bacteria clone 255 marine benthic group [%] (JTB255) * PI: Hoffmann, Katy (https://orcid.org/0000-0002-7714-4219, khoffman@mpi-bremen.de) * METHOD/DEVICE: Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH) (URI: https://doi.org/10.1128/AEM.68.6.3094-3101.2002) * COMMENT: minimum Japan Trench Bacteria clone 255 marine benthic group [%] (JTB255) * PI: Hoffmann, Katy (https://orcid.org/0000-0002-7714-4219, khoffman@mpi-bremen.de) * METHOD/DEVICE: Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH) (URI: https://doi.org/10.1128/AEM.68.6.3094-3101.2002) * COMMENT: maximum Replicates [#] (Repl) * PI: Hoffmann, Katy (https://orcid.org/0000-0002-7714-4219, khoffman@mpi-bremen.de) License: Creative Commons Attribution 3.0 Unported (CC-BY-3.0) (URI: https://creativecommons.org/licenses/by/3.0/) Size: 648 data points */ Event Latitude Longitude Date/Time Elevation [m] Sample ID Depth top [m] Depth bot [m] Depth sed [m] Env feature (biome) Env feature (material) Env feature (feature) Samp type Method Reference Comment Prokaryotes [#/ml] (minimum, Epifluorescence micr...) Prokaryotes [#/ml] (maximum, Epifluorescence micr...) Gammaproteob [10**3 #/ml] (minimum, Catalysed reporter d...) Gammaproteob [10**3 #/ml] (maximum, Catalysed reporter d...) Gammaproteobacteria [%] (minimum, Catalysed reporter d...) Gammaproteobacteria [%] (maximum, Catalysed reporter d...) JTB255 [#/ml] (minimum, Catalysed reporter d...) JTB255 [#/ml] (maximum, Catalysed reporter d...) JTB255 [%] (minimum, Catalysed reporter d...) JTB255 [%] (maximum, Catalysed reporter d...) Repl [#] PS80/350-1 87.9334 61.1696 2012-09-18T11:25 -4381 AO.1a 0.00 0.01 0.005 marine benthic biome(ENVO:01000024) marine sediment(ENVO:00002113) marine benthic feature(ENVO:01000105) surface sediment CARD-FISH this study below multi-year ice 618E6 658E6 3.26E3 3.47E3 500E-3 500E-3 6.42E6 6.94E6 1.0E0 1.1E0 2 PS80/350-1 87.9334 61.1696 2012-09-18T11:25 -4381 AO.1b 0.00 0.01 0.005 marine benthic biome(ENVO:01000024) marine sediment(ENVO:00002113) marine benthic feature(ENVO:01000105) surface sediment CARD-FISH this study below multi-year ice; sediment with algal aggregate on top 807E6 848E6 50.3E3 84.3E3 6.2E0 9.9E0 4.84E6 5.24E6 600E-3 600E-3 2 PS80/225-1 84.0473 31.1926 2012-08-09T10:35 -4019 AO.2 0.00 0.01 0.005 marine benthic biome(ENVO:01000024) marine sediment(ENVO:00002113) marine benthic feature(ENVO:01000105) surface sediment CARD-FISH this study ice edge 1.05E9 1.09E9 1.38E3 1.91E3 100E-3 200E-3 26.6E6 30.6E6 2.5E0 2.7E0 2 PS85/470-3 79.1335 6.1065 2014-06-24T23:34 -1244 AO.3a 0.00 0.01 0.005 marine benthic biome(ENVO:01000024) marine sediment(ENVO:00002113) marine benthic feature(ENVO:01000105) surface sediment CARD-FISH this study HGI 1.34E9 1.94E9 83.9E3 140E3 6.3E0 7.2E0 105E6 166E6 7.8E0 8.6E0 18 PS85/470-3 79.1335 6.1065 2014-06-24T23:34 -1244 AO.3b 0.02 0.03 0.025 marine benthic biome(ENVO:01000024) marine sediment(ENVO:00002113) marine benthic feature(ENVO:01000105) subsurface sediment CARD-FISH this study HGI 2.25E9 2.47E9 67.8E3 77.9E3 3.0E0 3.2E0 122E6 164E6 5.4E0 6.6E0 2 PS85/470-3 79.1335 6.1065 2014-06-24T23:34 -1244 AO.3c 0.04 0.05 0.045 marine benthic biome(ENVO:01000024) marine sediment(ENVO:00002113) marine benthic feature(ENVO:01000105) subsurface sediment CARD-FISH this study HGI 2.05E9 2.07E9 37.3E3 48.5E3 1.8E0 2.3E0 58.3E6 59.8E6 2.8E0 2.9E0 2 PS85/460-4 79.0652 4.1830 2014-06-22T18:47 -2403 AO.4a 0.00 0.01 0.005 marine benthic biome(ENVO:01000024) marine sediment(ENVO:00002113) marine benthic feature(ENVO:01000105) surface sediment CARD-FISH this study HGIV 1.29E9 1.61E9 32.4E3 62.5E3 2.5E0 3.9E0 52.4E6 112E6 4.1E0 7.0E0 12 PS85/464-1 79.0592 3.5728 2014-06-23T16:31 -3531 AO.5 0.00 0.01 0.005 marine benthic biome(ENVO:01000024) marine sediment(ENVO:00002113) marine benthic feature(ENVO:01000105) surface sediment CARD-FISH this study HGVI 765E6 796E6 11.8E3 21.9E3 1.5E0 2.8E0 56.8E6 59.8E6 7.4E0 7.5E0 2 PS85/465-4 79.1583 2.8828 2014-06-24T02:48 -5525 AO.6 0.00 0.01 0.005 marine benthic biome(ENVO:01000024) marine sediment(ENVO:00002113) marine benthic feature(ENVO:01000105) surface sediment CARD-FISH this study HGIX 753E6 1.19E9 15.1E3 36E3 2.0E0 3.0E0 39E6 91.7E6 5.2E0 7.7E0 4 PS85/436-1 78.9733 -5.2905 2014-06-17T14:30 -1056 AO.7 0.00 0.01 0.005 marine benthic biome(ENVO:01000024) marine sediment(ENVO:00002113) marine benthic feature(ENVO:01000105) surface sediment CARD-FISH this study EGI 1.03E9 1.06E9 44.2E3 54.6E3 4.3E0 5.2E0 85.2E6 88.2E6 8.3E0 8.3E0 2 PS85/454-3 78.5057 -2.8177 2014-06-20T17:59 -2558 AO.8 0.00 0.01 0.005 marine benthic biome(ENVO:01000024) marine sediment(ENVO:00002113) marine benthic feature(ENVO:01000105) surface sediment CARD-FISH this study EGIV 937E6 1.05E9 25.3E3 55.7E3 2.7E0 5.3E0 28.3E6 48.9E6 3.0E0 4.7E0 4 MSM04/3_251-ROV 14.6672 -45.0005 2007-01-29T13:17 -3021 NA.1 0.00 0.01 0.005 marine benthic biome(ENVO:01000024) marine sediment(ENVO:00002113) marine benthic feature(ENVO:01000105) surface sediment CARD-FISH Schauer et al. (2011) Oceanic soft sediment; control site; not seep-influenced 675E6 698E6 8.89E3 12.9E3 1.3E0 1.8E0 4.07E6 4.37E6 600E-3 600E-3 2 MSM04/3_259-ROV 14.7522 -44.9805 2007-02-01T13:20 -3032 NA.2 0.00 0.01 0.005 marine benthic biome(ENVO:01000024) marine sediment(ENVO:00002113) marine benthic feature(ENVO:01000105) surface sediment CARD-FISH Schauer et al. (2011) Logatchev hydrothermal vent field; Anya`s Garten; mat covered (elemental sulfur) 1.35E9 2.01E9 5.15E3 7.82E3 400E-3 400E-3 0.0E0 0.0E0 0.0E0 0.0E0 4 MSM04/3_271-ROV 14.7526 -44.9776 2007-02-05T13:08 -3042 NA.3 0.00 0.01 0.005 marine benthic biome(ENVO:01000024) marine sediment(ENVO:00002113) marine benthic feature(ENVO:01000105) surface sediment CARD-FISH Schauer et al. (2011) Logatchev hydrothermal vent field; Quest, near T-logger #19; mat-free 443E6 579E6 7.45E3 11.6E3 1.7E0 2.0E0 7.09E6 12.9E6 1.6E0 2.2E0 4 AT26-23-12 9.8833 -104.2333 -2800 NP.1 0.00 0.01 0.005 marine benthic biome(ENVO:01000024) marine sediment(ENVO:00002113) marine benthic feature(ENVO:01000105) surface sediment CARD-FISH S. Dyksma, unpublished data dive 4774; soft silt mud 1.47E9 1.53E9 8.18E3 21.9E3 600E-3 1.4E0 69E6 93E6 4.7E0 6.1E0 2 AT26-23-05 9.9000 -104.2667 -2600 NP.2a 0.00 0.01 0.005 marine benthic biome(ENVO:01000024) marine sediment(ENVO:00002113) marine benthic feature(ENVO:01000105) surface sediment CARD-FISH S. Dyksma, unpublished data dive 4774; soft silt mud 2.04E9 2.55E9 110E3 156E3 5.4E0 6.1E0 82.4E6 126E6 4.0E0 4.9E0 8 AT26-23-05 9.9000 -104.2667 -2600 NP.2b 0.10 0.12 0.110 marine benthic biome(ENVO:01000024) marine sediment(ENVO:00002113) marine benthic feature(ENVO:01000105) subsurface sediment CARD-FISH S. Dyksma, unpublished data dive 4774; soft silt mud 1.03E9 1.11E9 0.0E0 0.0E0 0.0E0 0.0E0 0.0E0 0.0E0 2 GeoB12202-1 24.7000 63.1667 2007-10-09T02:15 -1527 IO.1 0.00 0.02 0.010 marine benthic biome(ENVO:01000024) marine sediment(ENVO:00002113) marine benthic feature(ENVO:01000105) surface sediment CARD-FISH Sokoll et al. (2012) OMZ; N-limited; high seasonal surface production; GeoB St. No. 12202-1 1.23E9 1.27E9 60.1E3 93.1E3 4.9E0 7.3E0 49.1E6 50.1E6 4.0E0 3.9E0 2 GeoB12202-2 24.7000 63.1667 2007-10-09T03:50 -1526 IO.2 0.00 0.02 0.010 marine benthic biome(ENVO:01000024) marine sediment(ENVO:00002113) marine benthic feature(ENVO:01000105) surface sediment CARD-FISH Sokoll et al. (2012) OMZ; N-limited; high seasonal surface production;GeoB St. No. 12202-2 1.33E9 1.44E9 71.2E3 93.2E3 5.4E0 6.5E0 54.7E6 59.7E6 4.1E0 4.1E0 2 PS73/127-7 -47.9923 -16.0040 2009-01-31T03:51 -4077 SA.1a 0.00 0.02 0.010 marine benthic biome(ENVO:01000024) marine sediment(ENVO:00002113) marine benthic feature(ENVO:01000105) fluffy layer ontop of sediment CARD-FISH Thiele et al. (2015) iron fertilization experiment LOHAFEX; cold-core eddy 885E6 895E6 132E3 172E3 14.9E0 19.2E0 40.6E6 41.6E6 4.6E0 4.6E0 2 PS73/127-7 -47.9923 -16.0040 2009-01-31T03:51 -4077 SA.1b 0.00 0.01 0.005 marine benthic biome(ENVO:01000024) marine sediment(ENVO:00002113) marine benthic feature(ENVO:01000105) surface sediment CARD-FISH Thiele et al. (2015) iron fertilization experiment LOHAFEX; cold-core eddy 878E6 881E6 56.9E3 67.9E3 6.5E0 7.7E0 29.1E6 49.3E6 3.3E0 5.6E0 2 SO242/2_147-148 -7.1006 -88.4140 2015-09-02T00:00 -4198 SP.1 0.00 0.01 0.005 marine benthic biome(ENVO:01000024) marine sediment(ENVO:00002113) marine benthic feature(ENVO:01000105) surface sediment CARD-FISH this study DEA.W.undisturbed 663E6 826E6 6.46E3 8.74E3 1.0E0 1.1E0 13.1E6 21.8E6 2.0E0 2.6E0 4 SO242/2_194-1 -7.0761 -88.5266 2015-09-15T02:36 -4079 SP.2 0.00 0.01 0.005 marine benthic biome(ENVO:01000024) marine sediment(ENVO:00002113) marine benthic feature(ENVO:01000105) surface sediment CARD-FISH this study DEA.W.undisturbed 711E6 743E6 2.21E3 3.36E3 300E-3 500E-3 10.1E6 13.1E6 1.4E0 1.8E0 4 SO242/2_198_MUC -7.1256 -88.4509 2015-09-03T00:00 SP.6a 0.00 0.01 0.005 marine benthic biome(ENVO:01000024) marine sediment(ENVO:00002113) marine benthic feature(ENVO:01000105) polymetallic nodule CARD-FISH this study Ref.noduleOnTopOfSediment 453E6 555E6 4.04E3 5.11E3 900E-3 900E-3 0.0E0 0.0E0 0.0E0 0.0E0 2 SO242/2_198_MUC -7.1256 -88.4509 2015-09-03T00:00 SP.8a 0.00 0.01 0.005 marine benthic biome(ENVO:01000024) marine sediment(ENVO:00002113) marine benthic feature(ENVO:01000105) polymetallic nodule CARD-FISH this study Ref.noduleOnTopOfSediment 484E6 585E6 0.0E0 0.0E0 0.0E0 0.0E0 0.0E0 0.0E0 0.0E0 2 PS79/086-28 -51.9790 -12.0352 2012-02-01T18:59 AntO.1 0.00 0.01 0.005 marine benthic biome(ENVO:01000024) marine sediment(ENVO:00002113) marine benthic feature(ENVO:01000105) surface sediment CARD-FISH Ruff et al. (2014) B86; short exposure to bloom-derived material 394E6 492E6 23E3 32.2E3 5.8E0 6.5E0 19.7E6 32.8E6 5.0E0 6.7E0 8 PS79/141-9 -51.2672 -12.6177 2012-02-18T22:58 AntO.2 0.00 0.01 0.005 marine benthic biome(ENVO:01000024) marine sediment(ENVO:00002113) marine benthic feature(ENVO:01000105) surface sediment CARD-FISH Ruff et al. (2014) B141; long exposure to bloom-derived material 332E6 504E6 8.31E3 15.4E3 2.5E0 3.1E0 16.2E6 25.7E6 4.9E0 5.1E0 8 PS81/606-1 -51.4298 14.2223 2013-11-16T17:51 -4469 AntO.3d 0.00 0.01 0.005 marine benthic biome(ENVO:01000024) marine sediment(ENVO:00002113) marine benthic feature(ENVO:01000105) surface sediment CARD-FISH this study control outside the ridge valley 297E6 332E6 18E3 23.7E3 6.1E0 7.1E0 7.64E6 9.28E6 2.6E0 2.8E0 6 PS93/067-2 78.6280 10.5838 2015-08-06T17:23 -67 AO.9a 0.00 0.01 0.005 marine benthic biome(ENVO:01000024) marine sediment(ENVO:00002113) marine benthic feature(ENVO:01000105) surface sediment CARD-FISH this study FixO3 I 2.43E9 2.68E9 107E3 127E3 4.4E0 4.7E0 32.9E6 36.9E6 1.4E0 1.4E0 2 PS93/067-2 78.6280 10.5838 2015-08-06T17:23 -67 AO.9b 0.02 0.03 0.025 marine benthic biome(ENVO:01000024) marine sediment(ENVO:00002113) marine benthic feature(ENVO:01000105) subsurface sediment CARD-FISH this study FixO3 I 2.32E9 2.58E9 75.4E3 87E3 3.3E0 3.4E0 4.01E6 4.57E6 200E-3 200E-3 2 PS79/177-3 -53.8088 -52.3552 2012-03-07T13:22 -339 AntO.7 0.00 0.01 0.005 marine benthic biome(ENVO:01000024) marine sediment(ENVO:00002113) marine benthic feature(ENVO:01000105) surface sediment CARD-FISH Ruff et al. (2014) R177; reference station 605E6 2.36E9 37.3E3 87.4E3 3.7E0 6.2E0 24.5E6 111E6 4.0E0 4.7E0 6