Citation:

j.envexpbot.2016.09.001

Name: Low-steady-state metabolism induced by elevated CO2 increases resilience to UV radiation in the unicellular green-algae Dunaliella tertiolecta
Published: 2016
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chlorophyta; Dunaliella tertiolecta; Gene expression (incl. proteomics); Laboratory experiment; Laboratory strains; Light; Not applicable; Pelagos; Phytoplankton; Plantae; Primary production/Photosynthesis; Single species

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Abstract:

Global change factors derived from anthropogenic activities such as increased CO2 and ultraviolet radiation (UVR) have direct impacts on the physiological responses of organisms in both terrestrial and aquatic ecosystems. We assessed in this work the mechanistic, physiological and molecular response of the unicellular chlorophyte Dunaliella tertiolecta exposed to combinatorial present (390 ppmv, LC) and future CO2 levels predicted for the year 2100 (900 ppmv, HC) and different UVR doses. Growth rates, cell esterase activity, reactive oxygen species accumulation (ROS), and 14C fixation decreased under HC compared to LC. The deleterious effects of UVR were attenuated in HC, except for decreased photosynthetic capacities. Transcriptome analysis showed different expression patterns depending on UVR and CO2 levels. PsbA, LhcII and eCA gene expression was downregulated at HC compared to LC. However, photolyase (PL) gene expression was upregulated at HC with respect LC in UVR. This suggests that HC (as opposed to LC) promoted a "low-metabolic steady state", decreasing UVR exposure-activated repair by means of protein replacement. Only PL photoreactivation was active under HC. Our data add evidence on D. tertiolecta possibly becoming more resilient to UVR exposure under future CO2 regimes, and maybe other marine unicellular chlorophytes, warranting serious considerations on the consequent ecological implications.

Keyword(s):

Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chlorophyta; Dunaliella tertiolecta; Gene expression (incl. proteomics); Laboratory experiment; Laboratory strains; Light; Not applicable; Pelagos; Phytoplankton; Plantae; Primary production/Photosynthesis; Single species

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2017-03-16.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		García-Gómez, Candela		study
2	Species	Species		García-Gómez, Candela
3	Registration number of species	Reg spec no		García-Gómez, Candela
4	Uniform resource locator/link to reference	URL ref		García-Gómez, Candela		WoRMS Aphia ID
5	Identification	ID		García-Gómez, Candela		Cylinder
6	Treatment	Treat		García-Gómez, Candela
7	Time in days	Time	days	García-Gómez, Candela
8	Percentage	Perc	%	García-Gómez, Candela		Dead Cells
9	Cell density	Cells	#/ml	García-Gómez, Candela
10	Maximum photochemical quantum yield of photosystem II	Fv/Fm		García-Gómez, Candela
11	Fluorescence	Fluorescence	arbitrary units	García-Gómez, Candela		Cell esterase activity based on FDA green fluorescence emission
12	Reactive oxygen species production	ROS prod		García-Gómez, Candela
13	Gross carbon fixation rate per cell	G C fix/cell	fmol/#/day	García-Gómez, Candela
14	Gene expression	Gene expression		García-Gómez, Candela		PsbA
15	Gene expression	Gene expression		García-Gómez, Candela		LHCII
16	Gene expression	Gene expression		García-Gómez, Candela		DCA
17	Gene expression	Gene expression		García-Gómez, Candela		CPD photolyase
18	Salinity	Sal		García-Gómez, Candela
19	Temperature, water	Temp	°C	García-Gómez, Candela
20	Initial slope of the photosynthesis-irradiance curve	alpha	(mg C/mg Chl a/h)/(W/m²)	García-Gómez, Candela
21	Initial slope of rapid light curve, standard deviation	alpha std dev	±	García-Gómez, Candela
22	Maximal electron transport rate, relative	rETR max		García-Gómez, Candela
23	Maximal electron transport rate, relative, standard deviation	rETR max std dev	±	García-Gómez, Candela
24	Light saturation	Ek	µmol/m²/s	García-Gómez, Candela
25	Light saturation, standard deviation	Ek std dev	±	García-Gómez, Candela
26	pH	pH		García-Gómez, Candela		NBS scale
27	pH, standard deviation	pH std dev	±	García-Gómez, Candela		NBS scale
28	Carbon, inorganic, dissolved	DIC	µmol/l	García-Gómez, Candela
29	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	García-Gómez, Candela
30	Alkalinity, total	AT	µmol/l	García-Gómez, Candela
31	Alkalinity, total, standard deviation	AT std dev	±	García-Gómez, Candela
32	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
33	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
34	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
35	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
36	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
37	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
38	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
39	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
40	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
41	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1870 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 ID (Cylinder)	6 Treat	7 Time [days]	8 Perc [%] (Dead Cells)	9 Cells [#/ml]	10 Fv/Fm	11 Fluorescence [arbitrary units] (Cell esterase activity based ...)	12 ROS prod	13 G C fix/cell [fmol/#/day]	14 Gene expression (PsbA)	15 Gene expression (LHCII)	16 Gene expression (DCA)	17 Gene expression (CPD photolyase)	18 Sal	19 Temp [°C]	20 alpha [(mg C/mg Chl a/h)/(W/m²)]	21 alpha std dev [±]	22 rETR max	23 rETR max std dev [±]	24 Ek [µmol/m²/s]	25 Ek std dev [±]	26 pH (NBS scale)	27 pH std dev [±] (NBS scale)	28 DIC [µmol/l]	29 DIC std dev [±]	30 AT [µmol/l]	31 AT std dev [±]	32 CSC flag	33 pH (total scale)	34 CO2 [µmol/kg]	35 fCO2water_SST_wet [µatm]	36 pCO2water_SST_wet [µatm]	37 [HCO3]- [µmol/kg]	38 [CO3]2- [µmol/kg]	39 DIC [µmol/kg]	40 AT [µmol/kg]	41 Omega Arg	42 Omega Cal
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	1	HC-P	0	4.853	2528000	0.623	26.375	10.903	8.74					35	16							7.74	0.17	1867.48	90.23	1969.57	79.08	26	7.74	26.70	734.43	737.06	1730.50	73.69	1830.88	1920.13	1.13	1.76
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	2	HC-P	0	4.932	2632000	0.533	41.597	22.458	2.89					35	16							7.74	0.17	1867.48	90.23	1969.57	79.08	26	7.74	26.70	734.43	737.06	1730.50	73.69	1830.88	1920.13	1.13	1.76
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	3	HC-PAB	0	6.926	2528000	0.595	24.420	13.927	10.24	1.000	1.000	1.000	1.000	35	16							7.69	0.21	1907.55	130.93	1994.83	112.58	26	7.69	30.68	843.94	846.97	1772.20	67.25	1870.13	1944.76	1.03	1.60
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	4	HC-PAB	0	2.858	2832000	0.624	17.977	7.077	7.61	1.000	1.000	1.000	1.000	35	16							7.69	0.21	1907.55	130.93	1994.83	112.58	26	7.69	30.68	843.94	846.97	1772.20	67.25	1870.13	1944.76	1.03	1.60
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	5	LC-PAB	0	12.823	1000000	0.529	75.258	22.157	28.84	1.000	1.000	1.000	1.000	35	16							8.01	0.08	370.53	37.50	472.37	25.12	26	8.01	2.78	76.44	76.72	334.83	26.50	364.11	460.51	0.41	0.63
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	6	LC-PAB	0	13.953	1336000	0.511	40.696	20.765	24.05	1.000	1.000	1.000	1.000	35	16							8.01	0.08	370.53	37.50	472.37	25.12	26	8.01	2.78	76.44	76.72	334.83	26.50	364.11	460.51	0.41	0.63
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	7	LC-P	0	19.451	1080000	0.536	64.988	31.000	27.37	1.000	1.000	1.000	1.000	35	16							8.34	0.03	380.89	98.94	577.43	78.69	26	8.34	1.25	34.42	34.55	322.43	54.58	378.25	562.94	0.84	1.30
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	8	LC-P	0	8.021	1168000	0.573	61.615	23.667	27.37	1.000	1.000	1.000	1.000	35	16							8.34	0.03	380.89	98.94	577.43	78.69	26	8.34	1.25	34.42	34.55	322.43	54.58	378.25	562.94	0.84	1.30
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	1	HC-P	2	6.033	2640000	0.432	10.599	7.965	12.06					35	16							8.27	0.22	784.64	329.13	1010.00	282.84	26	8.27	3.07	84.40	84.70	673.49	97.12	773.68	984.65	1.49	2.32
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	2	HC-P	2	9.881	1968000	0.414	20.762	15.512	4.32					35	16							8.27	0.22	784.64	329.13	1010.00	282.84	26	8.27	3.07	84.40	84.70	673.49	97.12	773.68	984.65	1.49	2.32
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	3	HC-PAB	2	6.139	2256000	0.175	15.938	16.935	5.70	0.764	0.000	0.056	18.305	35	16							8.14	0.19	995.19	337.63	1190.00	296.98	26	8.14	5.40	148.60	149.13	879.21	94.01	978.62	1160.13	1.44	2.24
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	4	HC-PAB	2	5.298	2352000	0.155	13.146	9.558	3.18	0.851	0.000	0.045	18.305	35	16							8.14	0.19	995.19	337.63	1190.00	296.98	26	8.14	5.40	148.60	149.13	879.21	94.01	978.62	1160.13	1.44	2.24
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	5	LC-PAB	2	8.444	1584000	0.178	35.183	30.319	11.76	1.983	0.723	0.068	1.139	35	16							8.42	0.04	505.23	1.59	760.00	14.14	26	8.42	1.36	37.32	37.45	420.44	85.60	507.40	740.92	1.31	2.04
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	6	LC-PAB	2	7.911	1664000	0.202	45.710	35.565	9.84	2.341	1.406	0.237	2.064	35	16							8.42	0.04	505.23	1.59	760.00	14.14	26	8.42	1.36	37.32	37.45	420.44	85.60	507.40	740.92	1.31	2.04
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	7	LC-P	2	10.062	1296000	0.506	40.701	26.181	18.73	0.596	0.129	0.124		35	16							8.40	0.01	496.86	2.47	740.00	0.00	26	8.40	1.40	38.60	38.74	415.27	80.74	497.41	721.43	1.24	1.93
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	8	LC-P	2	7.449	1680000	0.524	37.867	23.673	16.31	0.914	0.075	0.070		35	16							8.40	0.01	496.86	2.47	740.00	0.00	26	8.40	1.40	38.60	38.74	415.27	80.74	497.41	721.43	1.24	1.93
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	1	HC-P	3		2944000	0.380
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	2	HC-P	3		2096000	0.350
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	3	HC-PAB	3		2272000	0.144				0.403	0.001	0.026	4.087
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	4	HC-PAB	3		2880000	0.105				0.264	0.001	0.050	6.892
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	5	LC-PAB	3		2144000	0.130				10.602	1.797	0.712	1.182
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	6	LC-PAB	3		1568000	0.166				6.623	1.688	0.429	1.957
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	7	LC-P	3		1288000	0.456				0.430	0.027	0.039
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	8	LC-P	3		1920000	0.472				0.343	0.036	0.037
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	1	HC-P	4	0.617	3456000	0.363	3.517	1.043	1.97					35	16							7.40	0.04	1064.72	429.94	1075.00	431.34	26	7.40	33.42	919.22	922.51	989.23	19.24	1041.89	1048.02	0.30	0.46
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	2	HC-P	4	0.621	2400000	0.259	4.224	-0.494	0.32					35	16							7.40	0.04	1064.72	429.94	1075.00	431.34	26	7.40	33.42	919.22	922.51	989.23	19.24	1041.89	1048.02	0.30	0.46
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	3	HC-PAB	4	-2.383	2560000	0.103	5.609	-0.469	0.73	1.027	0.001	0.054	16.830	35	16							7.42	0.01	1212.97	337.84	1225.00	332.34	26	7.42	36.36	1000.12	1003.71	1127.12	22.96	1186.43	1194.25	0.35	0.55
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	4	HC-PAB	4	5.216	2832000	0.094	4.129	0.492	0.41	0.863	0.003	0.054	23.264	35	16							7.42	0.01	1212.97	337.84	1225.00	332.34	26	7.42	36.36	1000.12	1003.71	1127.12	22.96	1186.43	1194.25	0.35	0.55
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	5	LC-PAB	4	12.830	2320000	0.103	10.628	3.805	4.67	19.263	3.336	2.800	3.844	35	16							8.48	0.35	532.67	94.84	795.00	7.07	26	8.48	1.17	32.21	32.33	416.70	97.42	515.29	775.04	1.50	2.32
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	6	LC-PAB	4	6.424	1920000	0.120	16.817	8.345	4.53	8.754	4.397	3.586	4.441	35	16							8.48	0.35	532.67	94.84	795.00	7.07	26	8.48	1.17	32.21	32.33	416.70	97.42	515.29	775.04	1.50	2.32
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	7	LC-P	4	8.434	1584000	0.433	29.533	17.747	16.00	0.127	0.010	0.020		35	16							8.46	0.35	463.96	86.98	720.00	56.57	26	8.46	1.11	30.41	30.51	375.58	83.84	460.52	701.93	1.29	2.00
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	8	LC-P	4	7.871	1696000	0.427	40.590	20.748	15.75	0.062	0.003	0.020		35	16							8.46	0.35	463.96	86.98	720.00	56.57	26	8.46	1.11	30.41	30.51	375.58	83.84	460.52	701.93	1.29	2.00
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	1	HC-P	6		2880000	0.304
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	2	HC-P	6		1760000	0.185
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	3	HC-PAB	6		2976000	0.071				1.691	0.002	0.292	6.474
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	4	HC-PAB	6		2832000	0.072				0.984	0.002	0.248	6.474
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	5	LC-PAB	6		2272000	0.097				18.808	2.194	0.862	6.197
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	6	LC-PAB	6		1944000	0.107				17.600	2.194	0.678	9.098
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	7	LC-P	6		2384000	0.404				0.434	0.009	0.016
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	8	LC-P	6		2016000	0.366				0.434	0.006	0.063
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	1	HC-P	7	2.354	3504000	0.287	0.694	0.694	0.83					35	16							7.61	0.09	1636.32	248.58	2000.00	282.84	26	7.61	37.55	1032.88	1036.58	1803.84	56.93	1898.32	1949.80	0.87	1.36
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	2	HC-P	7	4.075	1936000	0.173	0.000	0.000	0.47					35	16							7.61	0.09	1636.32	248.58	2000.00	282.84	26	7.61	37.55	1032.88	1036.58	1803.84	56.93	1898.32	1949.80	0.87	1.36
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	3	HC-PAB	7	4.632	2320000	0.352	1.749	1.749	1.53	0.482	0.009	0.116	13.778	35	16							7.73	0.04	1752.83	80.46	1850.00	70.71	26	7.73	25.68	706.55	709.08	1626.80	67.69	1720.17	1803.56	1.04	1.61
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	4	HC-PAB	7	6.430	2880000	0.375	1.117	1.117	1.69	0.523	0.016	0.059	9.057	35	16							7.73	0.04	1752.83	80.46	1850.00	70.71	26	7.73	25.68	706.55	709.08	1626.80	67.69	1720.17	1803.56	1.04	1.61
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	5	LC-PAB	7	11.622	2832000	0.328	6.953	6.953	13.19	0.474	2.075	1.907	6.427	35	16							8.55	0.09	440.40	59.73	740.00	28.28	26	8.55	0.83	22.96	23.04	348.92	95.83	445.58	721.43	1.47	2.29
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	6	LC-PAB	7	8.168	2272000	0.260	8.174	8.174	8.23	2.591	2.075	2.597	6.427	35	16							8.55	0.09	440.40	59.73	740.00	28.28	26	8.55	0.83	22.96	23.04	348.92	95.83	445.58	721.43	1.47	2.29
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	7	LC-P	7	7.604	2624000	0.380	16.538	16.538	23.92	1.000	2.000	239.472		35	16							8.38	0.07	471.90	18.28	705.00	49.50	26	8.38	1.41	38.84	38.97	398.99	74.08	474.48	687.30	1.14	1.77
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	8	LC-P	7	7.674	2624000	0.341	8.209	8.209	21.46	1.000	2.000	239.472		35	16							8.38	0.07	471.90	18.28	705.00	49.50	26	8.38	1.41	38.84	38.97	398.99	74.08	474.48	687.30	1.14	1.77
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	1	HC-P	8		2512000	0.254
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	2	HC-P	8		1920000	0.173
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	3	HC-PAB	8		2720000	0.312
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	4	HC-PAB	8		3280000	0.303
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	5	LC-PAB	8		2736000	0.316
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	6	LC-PAB	8		1904000	0.275
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	7	LC-P	8		2464000	0.349
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org	8	LC-P	8		2336000	0.313
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org		HC-P	0											35	16	2.52	0.53	163.97	25.91	67.64	18.31	7.74	0.17	1867.48	90.23	1969.57	79.08	26	7.74	26.70	734.43	737.06	1730.50	73.69	1830.88	1920.13	1.13	1.76
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org		HC-PAB	0											35	16	2.78	1.53	165.79	20.27	68.36	21.66	7.69	0.21	1907.55	130.93	1994.83	112.58	26	7.69	30.68	843.94	846.97	1772.20	67.25	1870.13	1944.76	1.03	1.60
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org		LC-P	0											35	16	5.57	1.54	173.24	24.65	29.06	9.55	8.34	0.03	380.89	98.94	577.43	78.69	26	8.34	1.25	34.42	34.55	322.43	54.58	378.25	562.94	0.84	1.30
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org		LC-PAB	0											35	16	3.74	1.62	144.72	67.81	36.56	5.49	8.01	0.08	370.53	37.50	472.37	25.12	26	8.01	2.78	76.44	76.72	334.83	26.50	364.11	460.51	0.41	0.63
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org		HC-P	2											35	16	2.95	1.04	178.74	62.48	54.37	23.83	8.27	0.22	784.64	329.13	1010.00	282.84	26	8.27	3.07	84.40	84.70	673.49	97.12	773.68	984.65	1.49	2.32
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org		HC-PAB	2											35	16	1.14	0.32	166.78	71.87	102.09	15.21	8.14	0.19	995.19	337.63	1190.00	296.98	26	8.14	5.40	148.60	149.13	879.21	94.01	978.62	1160.13	1.44	2.24
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org		LC-P	2											35	16	5.81	0.61	144.43	70.74	24.89	17.81	8.40	0.01	496.86	2.47	740.00	0.00	26	8.40	1.40	38.60	38.74	415.27	80.74	497.41	721.43	1.24	1.93
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org		LC-PAB	2											35	16	0.27	0.13	97.18	77.74	250.25	74.69	8.42	0.04	505.23	1.59	760.00	14.14	26	8.42	1.36	37.32	37.45	420.44	85.60	507.40	740.92	1.31	2.04
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org		HC-P	4											35	16	1.16	0.70	93.37	19.39	101.35	51.47	7.40	0.04	1064.72	429.94	1075.00	431.34	26	7.40	33.42	919.22	922.51	989.23	19.24	1041.89	1048.02	0.30	0.46
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org		HC-PAB	4											35	16	0.60	0.20	137.33	33.97	243.92	78.39	7.42	0.01	1212.97	337.84	1225.00	332.34	26	7.42	36.36	1000.12	1003.71	1127.12	22.96	1186.43	1194.25	0.35	0.55
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org		LC-P	4											35	16	1.28	0.79	205.42	95.31	191.92	75.53	8.46	0.35	463.96	86.98	720.00	56.57	26	8.46	1.11	30.41	30.51	375.58	83.84	460.52	701.93	1.29	2.00
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org		LC-PAB	4											35	16	0.74	0.10	180.55	58.33	251.61	98.70	8.48	0.35	532.67	94.84	795.00	7.07	26	8.48	1.17	32.21	32.33	416.70	97.42	515.29	775.04	1.50	2.32
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org		HC-P	7											35	16	1.15	0.39	147.25	38.43	136.53	46.98	7.61	0.09	1636.32	248.58	2000.00	282.84	26	7.61	37.55	1032.88	1036.58	1803.84	56.93	1898.32	1949.80	0.87	1.36
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org		HC-PAB	7											35	16	1.51	0.47	153.40	37.66	103.14	10.26	7.73	0.04	1752.83	80.46	1850.00	70.71	26	7.73	25.68	706.55	709.08	1626.80	67.69	1720.17	1803.56	1.04	1.61
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org		LC-P	7											35	16	1.47	0.36	249.81	59.37	184.47	78.56	8.38	0.07	471.90	18.28	705.00	49.50	26	8.38	1.41	38.84	38.97	398.99	74.08	474.48	687.30	1.14	1.77
laboratory	Dunaliella tertiolecta (phytoplankton)	178590	marinespecies.org		LC-PAB	7											35	16	2.47	0.82	192.86	43.52	81.01	16.37	8.55	0.09	440.40	59.73	740.00	28.28	26	8.55	0.83	22.96	23.04	348.92	95.83	445.58	721.43	1.47	2.29