Leo, Elettra; Kunz, Kristina Lore; Schmidt, Matthias; Storch, Daniela; Pörtner, Hans-Otto; Mark, Felix Christopher (2017): Individual mitochondrial functioning parameters from cardiac permeabilised fibers of Polar cod (Boreogadus saida) and Atlantic cod (Gadus morhua) acclimated to ocean acidification and warming. doi:10.1594/PANGAEA.873536, Supplement to: Leo, E et al. (2017): Mitochondrial acclimation potential to ocean acidification and warming of Polar cod (Boreogadus saida) and Atlantic cod (Gadus morhua). Frontiers in Zoology, 14(1), 12 pp, doi:10.1186/s12983-017-0205-1
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To investigate the effects of ocean acidification and warming on the cardiac mitochondrial metabolism of Polar cod (Boreogadus saida) and Atlantic cod (Gadus morhua), specimens of both species were acclimated to four temperatures (Polar cod: 0, 3, 6, 8°C; Atlantic cod: 3, 8, 12, 16°C) and two PCO2 (390 and 1170 µatm) for four months. After the incubation period, oxidative phosphorylation capacity OXPHOS (Respiratory State III), Respiratory State IV+ and Complex IV capacity (pmol O2 * mg fresh weight-1 * sec-1) of heart muscle of each individual were measured in permeabilised fibers. OXPHOS coupling efficiency ((OXPHOS - State IV+) * OXPHOS-1) and fractional State IV+ (% OXPHOS) were calculated.