Citation:

meps11828

Name: A longitudinal study of Pacific oyster (Crassostrea gigas) larval development: isotope shifts during early shell formation reveal sub-lethal energetic stress
Published: 2016
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Animalia; Biomass/Abundance/Elemental composition; Coast and continental shelf; Crassostrea gigas; Growth/Morphology; Laboratory experiment; Mesocosm or benthocosm; Mollusca; North Pacific; Pelagos; Single species; Temperate; Zooplankton

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Abstract:

Three cohorts of Pacific oyster (Crassostrea gigas) larvae at Whiskey Creek Shellfish Hatchery (WCH) in Netarts Bay, Oregon, were monitored for stable isotope incorporation and biochemical composition: one in May 2011 and two in August 2011. Along with measures of growth and calcification, we present measurements of stable isotopes of carbon in water, algal food, and the shell and tissue, and nitrogen in food and tissue across larval development and growth. These relatively unique measures through larval ontogeny allow us to document isotopic shifts associated with initiation and rate of feeding, and the catabolism of C-rich (lipid) and N-rich (protein) pools. Similar ontological patterns in growth and bulk composition among the cohorts reinforce prior results, suggesting that the creation of the initial shell is energetically expensive, that the major carbon source is ambient dissolved inorganic carbon, and that the major energetic source during this period is maternally derived egg lipids. The May cohort did not isotopically reflect its food source as rapidly as the August cohorts, indicating slower feeding and/or higher catabolism versus anabolism. Our measurements also document differences in bulk turnover of organic carbon and nitrogen pools within the larvae, showing far greater conservation of nitrogen than carbon. These stable isotope and bulk biochemical measurements appear to be more sensitive indicators of sub-lethal environmental stress than the commonly used metrics of development and growth.

Keyword(s):

Animalia; Biomass/Abundance/Elemental composition; Coast and continental shelf; Crassostrea gigas; Growth/Morphology; Laboratory experiment; Mesocosm or benthocosm; Mollusca; North Pacific; Pelagos; Single species; Temperate; Zooplankton

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2017-03-07.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Waldbusser, George G		study
2	Species	Species		Waldbusser, George G
3	Registration number of species	Reg spec no		Waldbusser, George G
4	Uniform resource locator/link to reference	URL ref		Waldbusser, George G		WoRMS Aphia ID
5	Experiment	Exp		Waldbusser, George G
6	Time in days	Time	days	Waldbusser, George G
7	Fraction	Fraction		Waldbusser, George G		lipid fraction of AFDW (organic material)
8	Fraction	Fraction		Waldbusser, George G		organic material (ash-free dry weight)
9	Length	l	µm	Waldbusser, George G
10	Length, standard deviation	l std dev	±	Waldbusser, George G
11	Dry mass per individual	dm/ind	µg/#	Waldbusser, George G		per larva
12	Lipids per individual	Lipid/ind	µg/#	Waldbusser, George G		per larva
13	Proportion	Prop		Waldbusser, George G		Total N
14	Proportion	Prop		Waldbusser, George G		Total C
15	Proportion	Prop		Waldbusser, George G		Organic N
16	Proportion	Prop		Waldbusser, George G		Organic C
17	Carbon/Nitrogen ratio	C/N		Waldbusser, George G		Tissue atomic
18	Calcification rate, ratio	Calc rate ratio		Waldbusser, George G		Mass-normalized calcification rate (/day)
19	δ13C	δ13C	‰ PDB	Waldbusser, George G		Tissue
20	δ15N	δ15N	‰ air	Waldbusser, George G		Tissue
21	δ13C	δ13C	‰ PDB	Waldbusser, George G		Algal
22	δ15N	δ15N	‰ air	Waldbusser, George G		Algal
23	δ13C	δ13C	‰ PDB	Waldbusser, George G		Shell
24	δ13C, dissolved inorganic carbon	δ13C DIC	‰ PDB	Waldbusser, George G
25	Temperature, water	Temp	°C	Waldbusser, George G
26	Temperature, water, standard deviation	Temp std dev	±	Waldbusser, George G
27	Salinity	Sal		Waldbusser, George G
28	Salinity, standard deviation	Sal std dev	±	Waldbusser, George G
29	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Waldbusser, George G
30	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Waldbusser, George G
31	Alkalinity, total	AT	µmol/kg	Waldbusser, George G
32	Alkalinity, total, standard deviation	AT std dev	±	Waldbusser, George G
33	Carbon, inorganic, dissolved	DIC	µmol/kg	Waldbusser, George G
34	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Waldbusser, George G
35	pH	pH		Waldbusser, George G
36	pH, standard deviation	pH std dev	±	Waldbusser, George G
37	Aragonite saturation state	Omega Arg		Waldbusser, George G
38	Aragonite saturation state, standard error	Omega Arg std e	±	Waldbusser, George G
39	Aragonite saturation state	Omega Arg		Waldbusser, George G
40	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
41	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
42	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
43	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
44	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
45	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
46	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
47	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
48	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

3504 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Exp	6 Time [days]	7 Fraction (lipid fraction of AFDW (organ...)	8 Fraction (organic material (ash-free dr...)	9 l [µm]	10 l std dev [±]	11 dm/ind [µg/#] (per larva)	12 Lipid/ind [µg/#] (per larva)	13 Prop (Total N)	14 Prop (Total C)	15 Prop (Organic N)	16 Prop (Organic C)	17 C/N (Tissue atomic)	18 Calc rate ratio (Mass-normalized calcification...)	19 δ13C [‰ PDB] (Tissue)	20 δ15N [‰ air] (Tissue)	21 δ13C [‰ PDB] (Algal)	22 δ15N [‰ air] (Algal)	23 δ13C [‰ PDB] (Shell)	24 δ13C DIC [‰ PDB]	25 Temp [°C]	26 Temp std dev [±]	27 Sal	28 Sal std dev [±]	29 pCO2water_SST_wet [µatm]	30 pCO2 std dev [±]	31 AT [µmol/kg]	32 AT std dev [±]	33 DIC [µmol/kg]	34 DIC std dev [±]	35 pH	36 pH std dev [±]	37 Omega Arg	38 Omega Arg std e [±]	39 Omega Arg	40 CSC flag	41 pH (total scale)	42 CO2 [µmol/kg]	43 fCO2water_SST_wet [µatm]	44 [HCO3]- [µmol/kg]	45 [CO3]2- [µmol/kg]	46 AT [µmol/kg]	47 Omega Arg	48 Omega Cal
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug Cohort	0	0.231	0.89																	23.9	1.71	32.7	0.6	1008	207	2318	151	2181	68	7.71	0.16	1.6	0.2	2.1	25	7.70	29.69	1004.59	2049.28	102.03	2297.68	1.63	2.50
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug Cohort	1	0.113	0.40	76	6	0.17200	0.01900	0.05	0.27	0.04	0.18	5.7	0.60							23.9	1.71	32.7	0.6	1008	207	2318	151	2181	68	7.71	0.16	1.6	0.2	2.1	25	7.70	29.69	1004.59	2049.28	102.03	2297.68	1.63	2.50
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug Cohort	3	0.043	0.20	83	6	0.22100	0.01000	0.02	0.19	0.02	0.08	5.2	0.17							23.9	1.71	32.7	0.6	1008	207	2318	151	2181	68	7.71	0.16	1.6	0.2	2.1	25	7.70	29.69	1004.59	2049.28	102.03	2297.68	1.63	2.50
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug Cohort	5	0.011	0.17	90	5	0.26600	0.00300	0.02	0.17	0.02	0.07	5.1	0.09							23.9	1.71	32.7	0.6	1008	207	2318	151	2181	68	7.71	0.16	1.6	0.2	2.1	25	7.70	29.69	1004.59	2049.28	102.03	2297.68	1.63	2.50
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug Cohort	7	0.011	0.16	92	6	0.28200	0.00300	0.02	0.17	0.02	0.07	4.8	0.02							23.9	1.71	32.7	0.6	1008	207	2318	151	2181	68	7.71	0.16	1.6	0.2	2.1	25	7.70	29.69	1004.59	2049.28	102.03	2297.68	1.63	2.50
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug Cohort	9	0.013	0.17	99	13	0.34100	0.00400	0.02	0.18	0.02	0.08	4.9	0.07							23.9	1.71	32.7	0.6	1008	207	2318	151	2181	68	7.71	0.16	1.6	0.2	2.1	25	7.70	29.69	1004.59	2049.28	102.03	2297.68	1.63	2.50
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug Cohort	11	0.019	0.20	122	21	0.58100	0.01100	0.02	0.19	0.02	0.08	5.0	0.15							23.9	1.71	32.7	0.6	1008	207	2318	151	2181	68	7.71	0.16	1.6	0.2	2.1	25	7.70	29.69	1004.59	2049.28	102.03	2297.68	1.63	2.50
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug Cohort	13	0.023	0.23	125	23	0.62500	0.01400	0.03	0.20	0.03	0.11	4.8	0.01							23.9	1.71	32.7	0.6	1008	207	2318	151	2181	68	7.71	0.16	1.6	0.2	2.1	25	7.70	29.69	1004.59	2049.28	102.03	2297.68	1.63	2.50
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug Cohort	15	0.015	0.23	151	36	1.00100	0.01500	0.03	0.20	0.03	0.10	4.5	0.15							23.9	1.71	32.7	0.6	1008	207	2318	151	2181	68	7.71	0.16	1.6	0.2	2.1	25	7.70	29.69	1004.59	2049.28	102.03	2297.68	1.63	2.50
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug Cohort	17	0.016	0.25	167	32	1.30600	0.02100	0.03	0.21	0.03	0.11	4.6	0.08							23.9	1.71	32.7	0.6	1008	207	2318	151	2181	68	7.71	0.16	1.6	0.2	2.1	25	7.70	29.69	1004.59	2049.28	102.03	2297.68	1.63	2.50
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug Cohort	19	0.023	0.27	182	40	1.62400	0.03700	0.03	0.21	0.03	0.12	4.7	0.07							23.9	1.71	32.7	0.6	1008	207	2318	151	2181	68	7.71	0.16	1.6	0.2	2.1	25	7.70	29.69	1004.59	2049.28	102.03	2297.68	1.63	2.50
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug Cohort	21	0.020	0.26	196	38	1.95900	0.03800	0.03	0.21	0.03	0.12	4.4	0.06							23.9	1.71	32.7	0.6	1008	207	2318	151	2181	68	7.71	0.16	1.6	0.2	2.1	25	7.70	29.69	1004.59	2049.28	102.03	2297.68	1.63	2.50
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug (buff) Cohort	0	0.246	0.92			0.00009	0.00049													23.9	1.71	32.8	0.6	425	45	2842	91	2452	81	8.09	0.04	4.2	0.3	4.6	25	8.10	12.51	423.56	2168.08	271.41	2807.97	4.34	6.64
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug (buff) Cohort	1	0.098	0.38	74	5	0.16200	0.01600	0.04	0.27	0.04	0.17	5.5	0.62							23.9	1.71	32.8	0.6	425	45	2842	91	2452	81	8.09	0.04	4.2	0.3	4.6	25	8.10	12.51	423.56	2168.08	271.41	2807.97	4.34	6.64
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug (buff) Cohort	3	0.035	0.18	85	7	0.23100	0.00800	0.02	0.18	0.02	0.08	4.7	0.19							23.9	1.71	32.8	0.6	425	45	2842	91	2452	81	8.09	0.04	4.2	0.3	4.6	25	8.10	12.51	423.56	2168.08	271.41	2807.97	4.34	6.64
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug (buff) Cohort	5	0.010	0.16	91	7	0.27900	0.00300	0.02	0.17	0.02	0.07	4.8	0.08							23.9	1.71	32.8	0.6	425	45	2842	91	2452	81	8.09	0.04	4.2	0.3	4.6	25	8.10	12.51	423.56	2168.08	271.41	2807.97	4.34	6.64
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug (buff) Cohort	7	0.012	0.16	94	9	0.29900	0.00400	0.02	0.20	0.02	0.07	4.5	0.03							23.9	1.71	32.8	0.6	425	45	2842	91	2452	81	8.09	0.04	4.2	0.3	4.6	25	8.10	12.51	423.56	2168.08	271.41	2807.97	4.34	6.64
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug (buff) Cohort	9	0.008	0.17	107	12	0.41800	0.00500	0.02	0.18	0.02	0.07	4.5	0.11							23.9	1.71	32.8	0.6	425	45	2842	91	2452	81	8.09	0.04	4.2	0.3	4.6	25	8.10	12.51	423.56	2168.08	271.41	2807.97	4.34	6.64
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug (buff) Cohort	11	0.015	0.19	118	20	0.53000	0.00800	0.02	0.18	0.02	0.08	4.7	0.08							23.9	1.71	32.8	0.6	425	45	2842	91	2452	81	8.09	0.04	4.2	0.3	4.6	25	8.10	12.51	423.56	2168.08	271.41	2807.97	4.34	6.64
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug (buff) Cohort	13	0.017	0.23	132	26	0.71500	0.01200	0.03	0.20	0.02	0.10	4.8	0.08							23.9	1.71	32.8	0.6	425	45	2842	91	2452	81	8.09	0.04	4.2	0.3	4.6	25	8.10	12.51	423.56	2168.08	271.41	2807.97	4.34	6.64
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug (buff) Cohort	15	0.014	0.23	156	35	1.09300	0.01600	0.03	0.20	0.03	0.10	4.6	0.13							23.9	1.71	32.8	0.6	425	45	2842	91	2452	81	8.09	0.04	4.2	0.3	4.6	25	8.10	12.51	423.56	2168.08	271.41	2807.97	4.34	6.64
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug (buff) Cohort	17	0.014	0.25	173	38	1.43000	0.02100	0.03	0.20	0.03	0.11	4.6	0.08							23.9	1.71	32.8	0.6	425	45	2842	91	2452	81	8.09	0.04	4.2	0.3	4.6	25	8.10	12.51	423.56	2168.08	271.41	2807.97	4.34	6.64
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug (buff) Cohort	19	0.017	0.25	201	43	2.10200	0.03600	0.03	0.21	0.03	0.11	4.5	0.12							23.9	1.71	32.8	0.6	425	45	2842	91	2452	81	8.09	0.04	4.2	0.3	4.6	25	8.10	12.51	423.56	2168.08	271.41	2807.97	4.34	6.64
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug (buff) Cohort	21	0.014	0.26	214	45	2.44500	0.03500	0.03	0.21	0.03	0.12	4.4	0.05							23.9	1.71	32.8	0.6	425	45	2842	91	2452	81	8.09	0.04	4.2	0.3	4.6	25	8.10	12.51	423.56	2168.08	271.41	2807.97	4.34	6.64
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	May Cohort	2	0.023	0.12	77	5	0.17900	0.00400	0.01	0.16	0.01	0.05	5.8	0.44							23.9	1.71	26.6	2.3	364	40	1991	78	1758	50	8.04	0.04	2.6	0.3	2.4	25	8.05	11.07	362.77	1595.41	151.52	1965.74	2.49	3.88
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	May Cohort	5	0.010	0.08	81	4	0.20500	0.00200	0.01	0.14	0.01	0.03	4.8	0.05							23.9	1.71	26.6	2.3	364	40	1991	78	1758	50	8.04	0.04	2.6	0.3	2.4	25	8.05	11.07	362.77	1595.41	151.52	1965.74	2.49	3.88
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	May Cohort	8	0.010	0.10	89	7	0.26400	0.00300	0.01	0.15	0.01	0.04	4.5	0.06							23.9	1.71	26.6	2.3	364	40	1991	78	1758	50	8.04	0.04	2.6	0.3	2.4	25	8.05	11.07	362.77	1595.41	151.52	1965.74	2.49	3.88
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	May Cohort	11	0.017	0.14	106	10	0.41100	0.00700	0.02	0.17	0.02	0.06	4.4	0.09							23.9	1.71	26.6	2.3	364	40	1991	78	1758	50	8.04	0.04	2.6	0.3	2.4	25	8.05	11.07	362.77	1595.41	151.52	1965.74	2.49	3.88
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	May Cohort	13	0.020	0.18	126	15	0.63100	0.01300	0.02	0.18	0.02	0.08	4.9	0.13							23.9	1.71	26.6	2.3	364	40	1991	78	1758	50	8.04	0.04	2.6	0.3	2.4	25	8.05	11.07	362.77	1595.41	151.52	1965.74	2.49	3.88
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	May Cohort	15	0.019	0.16	135	17	0.75600	0.01400	0.02	0.18	0.02	0.07	4.9	0.08							23.9	1.71	26.6	2.3	364	40	1991	78	1758	50	8.04	0.04	2.6	0.3	2.4	25	8.05	11.07	362.77	1595.41	151.52	1965.74	2.49	3.88
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	May Cohort	17	0.033	0.25	144	23	0.99000	0.03300	0.03	0.21	0.02	0.10	4.9	0.05							23.9	1.71	26.6	2.3	364	40	1991	78	1758	50	8.04	0.04	2.6	0.3	2.4	25	8.05	11.07	362.77	1595.41	151.52	1965.74	2.49	3.88
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	May Cohort	19	0.047	0.32	203	40	2.14200	0.10100	0.04	0.24	0.03	0.15	5.7	0.17							23.9	1.71	26.6	2.3	364	40	1991	78	1758	50	8.04	0.04	2.6	0.3	2.4	25	8.05	11.07	362.77	1595.41	151.52	1965.74	2.49	3.88
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	May Cohort	0																			23.9	1.71	26.6	2.3	364	40	1991	78	1758	50	8.04	0.04	2.6	0.3	2.4	25	8.05	11.07	362.77	1595.41	151.52	1965.74	2.49	3.88
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	May Cohort	1																			23.9	1.71	26.6	2.3	364	40	1991	78	1758	50	8.04	0.04	2.6	0.3	2.4	25	8.05	11.07	362.77	1595.41	151.52	1965.74	2.49	3.88
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	May Cohort	2													-19.55	9.58	-39.47	-8.66	1.33	-0.04	23.9	1.71	26.6	2.3	364	40	1991	78	1758	50	8.04	0.04	2.6	0.3	2.4	25	8.05	11.07	362.77	1595.41	151.52	1965.74	2.49	3.88
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	May Cohort	3															-46.58	-7.60			23.9	1.71	26.6	2.3	364	40	1991	78	1758	50	8.04	0.04	2.6	0.3	2.4	25	8.05	11.07	362.77	1595.41	151.52	1965.74	2.49	3.88
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	May Cohort	4															-46.42	-7.52			23.9	1.71	26.6	2.3	364	40	1991	78	1758	50	8.04	0.04	2.6	0.3	2.4	25	8.05	11.07	362.77	1595.41	151.52	1965.74	2.49	3.88
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	May Cohort	5													-23.60	5.05	-46.49	-7.21	0.96	-0.04	23.9	1.71	26.6	2.3	364	40	1991	78	1758	50	8.04	0.04	2.6	0.3	2.4	25	8.05	11.07	362.77	1595.41	151.52	1965.74	2.49	3.88
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	May Cohort	6															-45.38	-5.67			23.9	1.71	26.6	2.3	364	40	1991	78	1758	50	8.04	0.04	2.6	0.3	2.4	25	8.05	11.07	362.77	1595.41	151.52	1965.74	2.49	3.88
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	May Cohort	7															-45.79	-5.82			23.9	1.71	26.6	2.3	364	40	1991	78	1758	50	8.04	0.04	2.6	0.3	2.4	25	8.05	11.07	362.77	1595.41	151.52	1965.74	2.49	3.88
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	May Cohort	8													-30.80	2.00	-45.42	-4.96	-0.18	-0.61	23.9	1.71	26.6	2.3	364	40	1991	78	1758	50	8.04	0.04	2.6	0.3	2.4	25	8.05	11.07	362.77	1595.41	151.52	1965.74	2.49	3.88
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	May Cohort	9															-27.85	-10.44			23.9	1.71	26.6	2.3	364	40	1991	78	1758	50	8.04	0.04	2.6	0.3	2.4	25	8.05	11.07	362.77	1595.41	151.52	1965.74	2.49	3.88
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	May Cohort	10															-33.89	-12.71			23.9	1.71	26.6	2.3	364	40	1991	78	1758	50	8.04	0.04	2.6	0.3	2.4	25	8.05	11.07	362.77	1595.41	151.52	1965.74	2.49	3.88
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	May Cohort	11													-30.32	-0.73	-29.04	-9.74	-0.95	-0.15	23.9	1.71	26.6	2.3	364	40	1991	78	1758	50	8.04	0.04	2.6	0.3	2.4	25	8.05	11.07	362.77	1595.41	151.52	1965.74	2.49	3.88
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	May Cohort	12															-26.89	-5.11			23.9	1.71	26.6	2.3	364	40	1991	78	1758	50	8.04	0.04	2.6	0.3	2.4	25	8.05	11.07	362.77	1595.41	151.52	1965.74	2.49	3.88
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	May Cohort	13													-28.14	3.34	-26.76	-5.60	-1.03	0.10	23.9	1.71	26.6	2.3	364	40	1991	78	1758	50	8.04	0.04	2.6	0.3	2.4	25	8.05	11.07	362.77	1595.41	151.52	1965.74	2.49	3.88
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	May Cohort	14															-34.38	-9.35			23.9	1.71	26.6	2.3	364	40	1991	78	1758	50	8.04	0.04	2.6	0.3	2.4	25	8.05	11.07	362.77	1595.41	151.52	1965.74	2.49	3.88
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	May Cohort	15													-28.10	3.09	-38.49	-11.41	-1.00	0.37	23.9	1.71	26.6	2.3	364	40	1991	78	1758	50	8.04	0.04	2.6	0.3	2.4	25	8.05	11.07	362.77	1595.41	151.52	1965.74	2.49	3.88
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	May Cohort	16															-43.19	-7.81			23.9	1.71	26.6	2.3	364	40	1991	78	1758	50	8.04	0.04	2.6	0.3	2.4	25	8.05	11.07	362.77	1595.41	151.52	1965.74	2.49	3.88
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	May Cohort	17													-32.95	5.18	-31.95	-4.75	-1.40	0.18	23.9	1.71	26.6	2.3	364	40	1991	78	1758	50	8.04	0.04	2.6	0.3	2.4	25	8.05	11.07	362.77	1595.41	151.52	1965.74	2.49	3.88
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	May Cohort	18														4.92	-35.38	-12.15			23.9	1.71	26.6	2.3	364	40	1991	78	1758	50	8.04	0.04	2.6	0.3	2.4	25	8.05	11.07	362.77	1595.41	151.52	1965.74	2.49	3.88
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	May Cohort	19													-27.31	2.64			-1.05		23.9	1.71	26.6	2.3	364	40	1991	78	1758	50	8.04	0.04	2.6	0.3	2.4	25	8.05	11.07	362.77	1595.41	151.52	1965.74	2.49	3.88
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	May Cohort	20																			23.9	1.71	26.6	2.3	364	40	1991	78	1758	50	8.04	0.04	2.6	0.3	2.4	25	8.05	11.07	362.77	1595.41	151.52	1965.74	2.49	3.88
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	May Cohort	21																			23.9	1.71	26.6	2.3	364	40	1991	78	1758	50	8.04	0.04	2.6	0.3	2.4	25	8.05	11.07	362.77	1595.41	151.52	1965.74	2.49	3.88
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug Cohort	0													-20.00	9.87				-0.12	23.9	1.71	32.7	0.6	1008	207	2318	151	2181	68	7.71	0.16	1.6	0.2	2.1	25	7.70	29.69	1004.59	2049.28	102.03	2297.68	1.63	2.50
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug Cohort	1													-19.48	10.10	-35.77	-6.48	0.75	-0.03	23.9	1.71	32.7	0.6	1008	207	2318	151	2181	68	7.71	0.16	1.6	0.2	2.1	25	7.70	29.69	1004.59	2049.28	102.03	2297.68	1.63	2.50
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug Cohort	2															-50.19	-2.81			23.9	1.71	32.7	0.6	1008	207	2318	151	2181	68	7.71	0.16	1.6	0.2	2.1	25	7.70	29.69	1004.59	2049.28	102.03	2297.68	1.63	2.50
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug Cohort	3													-29.46	5.07	-47.15	-2.96	-0.04	0.03	23.9	1.71	32.7	0.6	1008	207	2318	151	2181	68	7.71	0.16	1.6	0.2	2.1	25	7.70	29.69	1004.59	2049.28	102.03	2297.68	1.63	2.50
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug Cohort	4															-47.01	-2.79			23.9	1.71	32.7	0.6	1008	207	2318	151	2181	68	7.71	0.16	1.6	0.2	2.1	25	7.70	29.69	1004.59	2049.28	102.03	2297.68	1.63	2.50
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug Cohort	5													-32.84	3.97	-47.13	-3.59	-0.13	-0.17	23.9	1.71	32.7	0.6	1008	207	2318	151	2181	68	7.71	0.16	1.6	0.2	2.1	25	7.70	29.69	1004.59	2049.28	102.03	2297.68	1.63	2.50
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug Cohort	6															-30.16	-5.60			23.9	1.71	32.7	0.6	1008	207	2318	151	2181	68	7.71	0.16	1.6	0.2	2.1	25	7.70	29.69	1004.59	2049.28	102.03	2297.68	1.63	2.50
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug Cohort	7													-34.17	2.94	-32.97	-6.15	-0.25	-0.29	23.9	1.71	32.7	0.6	1008	207	2318	151	2181	68	7.71	0.16	1.6	0.2	2.1	25	7.70	29.69	1004.59	2049.28	102.03	2297.68	1.63	2.50
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug Cohort	8															-39.91	-5.75			23.9	1.71	32.7	0.6	1008	207	2318	151	2181	68	7.71	0.16	1.6	0.2	2.1	25	7.70	29.69	1004.59	2049.28	102.03	2297.68	1.63	2.50
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug Cohort	9													-32.92	1.05	-37.57	-5.74	-0.43	-0.31	23.9	1.71	32.7	0.6	1008	207	2318	151	2181	68	7.71	0.16	1.6	0.2	2.1	25	7.70	29.69	1004.59	2049.28	102.03	2297.68	1.63	2.50
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug Cohort	10															-35.82	-6.04			23.9	1.71	32.7	0.6	1008	207	2318	151	2181	68	7.71	0.16	1.6	0.2	2.1	25	7.70	29.69	1004.59	2049.28	102.03	2297.68	1.63	2.50
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug Cohort	11													-33.15	0.47	-32.48	-6.24	-0.79	-0.49	23.9	1.71	32.7	0.6	1008	207	2318	151	2181	68	7.71	0.16	1.6	0.2	2.1	25	7.70	29.69	1004.59	2049.28	102.03	2297.68	1.63	2.50
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug Cohort	12															-50.96	-3.09			23.9	1.71	32.7	0.6	1008	207	2318	151	2181	68	7.71	0.16	1.6	0.2	2.1	25	7.70	29.69	1004.59	2049.28	102.03	2297.68	1.63	2.50
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug Cohort	13													-34.23	0.99	-40.37	-6.50	-1.34	-0.08	23.9	1.71	32.7	0.6	1008	207	2318	151	2181	68	7.71	0.16	1.6	0.2	2.1	25	7.70	29.69	1004.59	2049.28	102.03	2297.68	1.63	2.50
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug Cohort	14															-53.80	-4.59			23.9	1.71	32.7	0.6	1008	207	2318	151	2181	68	7.71	0.16	1.6	0.2	2.1	25	7.70	29.69	1004.59	2049.28	102.03	2297.68	1.63	2.50
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug Cohort	15													-34.76	1.79	-37.70	-7.59	-1.50	-0.27	23.9	1.71	32.7	0.6	1008	207	2318	151	2181	68	7.71	0.16	1.6	0.2	2.1	25	7.70	29.69	1004.59	2049.28	102.03	2297.68	1.63	2.50
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug Cohort	16															-33.32	-4.69			23.9	1.71	32.7	0.6	1008	207	2318	151	2181	68	7.71	0.16	1.6	0.2	2.1	25	7.70	29.69	1004.59	2049.28	102.03	2297.68	1.63	2.50
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug Cohort	17													-33.90	1.83	-33.32	-5.17	-1.62	-0.09	23.9	1.71	32.7	0.6	1008	207	2318	151	2181	68	7.71	0.16	1.6	0.2	2.1	25	7.70	29.69	1004.59	2049.28	102.03	2297.68	1.63	2.50
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug Cohort	18															-34.73	-4.52			23.9	1.71	32.7	0.6	1008	207	2318	151	2181	68	7.71	0.16	1.6	0.2	2.1	25	7.70	29.69	1004.59	2049.28	102.03	2297.68	1.63	2.50
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug Cohort	19													-33.18	2.37	-44.41	-4.32	-1.69	-0.02	23.9	1.71	32.7	0.6	1008	207	2318	151	2181	68	7.71	0.16	1.6	0.2	2.1	25	7.70	29.69	1004.59	2049.28	102.03	2297.68	1.63	2.50
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug Cohort	20															-36.64	-5.64			23.9	1.71	32.7	0.6	1008	207	2318	151	2181	68	7.71	0.16	1.6	0.2	2.1	25	7.70	29.69	1004.59	2049.28	102.03	2297.68	1.63	2.50
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug Cohort	21													-33.97	2.81			-1.90		23.9	1.71	32.7	0.6	1008	207	2318	151	2181	68	7.71	0.16	1.6	0.2	2.1	25	7.70	29.69	1004.59	2049.28	102.03	2297.68	1.63	2.50
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug (buff) Cohort	0													-20.00	9.87				-0.52	23.9	1.71	32.8	0.6	425	45	2842	91	2452	81	8.09	0.04	4.2	0.3	4.6	25	8.10	12.51	423.56	2168.08	271.41	2807.97	4.34	6.64
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug (buff) Cohort	1													-19.38	10.12			0.65	-0.46	23.9	1.71	32.8	0.6	425	45	2842	91	2452	81	8.09	0.04	4.2	0.3	4.6	25	8.10	12.51	423.56	2168.08	271.41	2807.97	4.34	6.64
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug (buff) Cohort	2																			23.9	1.71	32.8	0.6	425	45	2842	91	2452	81	8.09	0.04	4.2	0.3	4.6	25	8.10	12.51	423.56	2168.08	271.41	2807.97	4.34	6.64
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug (buff) Cohort	3													-29.73	4.98			-0.44	-0.52	23.9	1.71	32.8	0.6	425	45	2842	91	2452	81	8.09	0.04	4.2	0.3	4.6	25	8.10	12.51	423.56	2168.08	271.41	2807.97	4.34	6.64
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug (buff) Cohort	4																			23.9	1.71	32.8	0.6	425	45	2842	91	2452	81	8.09	0.04	4.2	0.3	4.6	25	8.10	12.51	423.56	2168.08	271.41	2807.97	4.34	6.64
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug (buff) Cohort	5													-33.57	3.62			-0.72	-0.77	23.9	1.71	32.8	0.6	425	45	2842	91	2452	81	8.09	0.04	4.2	0.3	4.6	25	8.10	12.51	423.56	2168.08	271.41	2807.97	4.34	6.64
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug (buff) Cohort	6																			23.9	1.71	32.8	0.6	425	45	2842	91	2452	81	8.09	0.04	4.2	0.3	4.6	25	8.10	12.51	423.56	2168.08	271.41	2807.97	4.34	6.64
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug (buff) Cohort	7													-34.44	2.11			-1.03	-0.81	23.9	1.71	32.8	0.6	425	45	2842	91	2452	81	8.09	0.04	4.2	0.3	4.6	25	8.10	12.51	423.56	2168.08	271.41	2807.97	4.34	6.64
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug (buff) Cohort	8																			23.9	1.71	32.8	0.6	425	45	2842	91	2452	81	8.09	0.04	4.2	0.3	4.6	25	8.10	12.51	423.56	2168.08	271.41	2807.97	4.34	6.64
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug (buff) Cohort	9													-32.96	0.47			-1.26	-1.06	23.9	1.71	32.8	0.6	425	45	2842	91	2452	81	8.09	0.04	4.2	0.3	4.6	25	8.10	12.51	423.56	2168.08	271.41	2807.97	4.34	6.64
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug (buff) Cohort	10																			23.9	1.71	32.8	0.6	425	45	2842	91	2452	81	8.09	0.04	4.2	0.3	4.6	25	8.10	12.51	423.56	2168.08	271.41	2807.97	4.34	6.64
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug (buff) Cohort	11													-32.95	0.96			-1.61	-0.92	23.9	1.71	32.8	0.6	425	45	2842	91	2452	81	8.09	0.04	4.2	0.3	4.6	25	8.10	12.51	423.56	2168.08	271.41	2807.97	4.34	6.64
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug (buff) Cohort	12																			23.9	1.71	32.8	0.6	425	45	2842	91	2452	81	8.09	0.04	4.2	0.3	4.6	25	8.10	12.51	423.56	2168.08	271.41	2807.97	4.34	6.64
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug (buff) Cohort	13													-33.94	1.38			-2.14	-0.56	23.9	1.71	32.8	0.6	425	45	2842	91	2452	81	8.09	0.04	4.2	0.3	4.6	25	8.10	12.51	423.56	2168.08	271.41	2807.97	4.34	6.64
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug (buff) Cohort	14																			23.9	1.71	32.8	0.6	425	45	2842	91	2452	81	8.09	0.04	4.2	0.3	4.6	25	8.10	12.51	423.56	2168.08	271.41	2807.97	4.34	6.64
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug (buff) Cohort	15													-34.66	2.08			-2.35	-0.97	23.9	1.71	32.8	0.6	425	45	2842	91	2452	81	8.09	0.04	4.2	0.3	4.6	25	8.10	12.51	423.56	2168.08	271.41	2807.97	4.34	6.64
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug (buff) Cohort	16																			23.9	1.71	32.8	0.6	425	45	2842	91	2452	81	8.09	0.04	4.2	0.3	4.6	25	8.10	12.51	423.56	2168.08	271.41	2807.97	4.34	6.64
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug (buff) Cohort	17													-33.74	2.19			-2.60	-0.58	23.9	1.71	32.8	0.6	425	45	2842	91	2452	81	8.09	0.04	4.2	0.3	4.6	25	8.10	12.51	423.56	2168.08	271.41	2807.97	4.34	6.64
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug (buff) Cohort	18																			23.9	1.71	32.8	0.6	425	45	2842	91	2452	81	8.09	0.04	4.2	0.3	4.6	25	8.10	12.51	423.56	2168.08	271.41	2807.97	4.34	6.64
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug (buff) Cohort	19													-33.14	2.61			-2.74	-0.51	23.9	1.71	32.8	0.6	425	45	2842	91	2452	81	8.09	0.04	4.2	0.3	4.6	25	8.10	12.51	423.56	2168.08	271.41	2807.97	4.34	6.64
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug (buff) Cohort	20																			23.9	1.71	32.8	0.6	425	45	2842	91	2452	81	8.09	0.04	4.2	0.3	4.6	25	8.10	12.51	423.56	2168.08	271.41	2807.97	4.34	6.64
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Aug (buff) Cohort	21													-34.04	2.94			-2.91		23.9	1.71	32.8	0.6	425	45	2842	91	2452	81	8.09	0.04	4.2	0.3	4.6	25	8.10	12.51	423.56	2168.08	271.41	2807.97	4.34	6.64