Citation:

s00227-016-2984-x

Name: Metabolic responses to high pCO2 conditions at a CO2 vent site in juveniles of a marine isopod species assemblage
Published: 2016
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Animalia; Arthropoda; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); CO2 vent; Coast and continental shelf; Cymodoce truncata; Dynamene bifida; Dynamene torelliae; Field experiment; Mediterranean Sea; Other metabolic rates; Single species; Temperate

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Abstract:

We are starting to understand the relationship between metabolic rate responses and species' ability to respond to exposure to high pCO2. However, most of our knowledge has come from investigations of single species. The examination of metabolic responses of closely related species with differing distributions around natural elevated CO2 areas may be useful to inform our understanding of their adaptive significance. Furthermore, little is known about the physiological responses of marine invertebrate juveniles to high pCO2, despite the fact they are known to be sensitive to other stressors, often acting as bottlenecks for future species success. We conducted an in situ transplant experiment using juveniles of isopods found living inside and around a high pCO2 vent (Ischia, Italy): the CO2 'tolerant' Dynamene bifida and 'sensitive' Cymodoce truncata and Dynamene torelliae. This allowed us to test for any generality of the hypothesis that pCO2 sensitive marine invertebrates may be those that experience trade-offs between energy metabolism and cellular homoeostasis under high pCO2 conditions. Both sensitive species were able to maintain their energy metabolism under high pCO2 conditions, but in C. truncata this may occur at the expense of [carbonic anhydrase], confirming our hypothesis. By comparison, the tolerant D. bifida appeared metabolically well adapted to high pCO2, being able to upregulate ATP production without recourse to anaerobiosis. These isopods are important keystone species; however, given they differ in their metabolic responses to future pCO2, shifts in the structure of the marine ecosystems they inhabit may be expected under future ocean acidification conditions.

Keyword(s):

Animalia; Arthropoda; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); CO2 vent; Coast and continental shelf; Cymodoce truncata; Dynamene bifida; Dynamene torelliae; Field experiment; Mediterranean Sea; Other metabolic rates; Single species; Temperate

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Median Latitude: 40.734723 * Median Longitude: 13.955650 * South-bound Latitude: 40.726110 * West-bound Longitude: 13.944170 * North-bound Latitude: 40.746670 * East-bound Longitude: 13.963060

Event(s):

Castello_Aragonese_south * Latitude: 40.731390 * Longitude: 13.963060 * Method/Device: Experiment (EXP)

San_Pietro_promontory * Latitude: 40.746670 * Longitude: 13.944170 * Method/Device: Experiment (EXP)

St_Anna_rocks * Latitude: 40.726110 * Longitude: 13.959720 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2017-03-07.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Event label	Event		Turner, Lucy M
2	Type	Type		Turner, Lucy M		study
3	Species	Species		Turner, Lucy M
4	Registration number of species	Reg spec no		Turner, Lucy M
5	Uniform resource locator/link to reference	URL ref		Turner, Lucy M		WoRMS Aphia ID
6	Treatment	Treat		Turner, Lucy M
7	Adenosine triphosphate, per unit protein	ATP	nmol/mg	Turner, Lucy M
8	Adenosine 5-Triphosphate, standard error	ATP std e	±	Turner, Lucy M
9	Replicates	Repl	#	Turner, Lucy M		ATP
10	L-lactate	L-lactate	mol/mg	Turner, Lucy M
11	L-lactate, standard error	L-lactate std e	±	Turner, Lucy M
12	Replicates	Repl	#	Turner, Lucy M		l-lactate
13	Carbonic anhydrase activity, per protein	CA act/prot	U/g	Turner, Lucy M
14	Carbonic anhydrase activity, standard error	CA act std e	±	Turner, Lucy M
15	Replicates	Repl	#	Turner, Lucy M		Carbonic anhydrase
16	Salinity	Sal		Turner, Lucy M
17	Salinity, standard error	Sal std e	±	Turner, Lucy M
18	Temperature, water	Temp	°C	Turner, Lucy M
19	Temperature, water, standard error	T std e	±	Turner, Lucy M
20	pH	pH		Turner, Lucy M		NBS scale
21	pH, standard error	pH std e	±	Turner, Lucy M		NBS scale
22	Alkalinity, total	AT	µmol/kg	Turner, Lucy M
23	Alkalinity, total, standard error	AT std e	±	Turner, Lucy M
24	Carbon, inorganic, dissolved	DIC	µmol/kg	Turner, Lucy M	Calculated using CO2SYS
25	Carbon, inorganic, dissolved, standard error	DIC std e	±	Turner, Lucy M	Calculated using CO2SYS
26	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Turner, Lucy M	Calculated using CO2SYS
27	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error	pCO2water_SST_wet std e	±	Turner, Lucy M	Calculated using CO2SYS
28	Bicarbonate ion	[HCO3]-	µmol/kg	Turner, Lucy M	Calculated using CO2SYS
29	Bicarbonate ion, standard error	[HCO3]- std e	±	Turner, Lucy M	Calculated using CO2SYS
30	Carbonate ion	[CO3]2-	µmol/kg	Turner, Lucy M	Calculated using CO2SYS
31	Carbonate ion, standard error	[CO3]2- std e	±	Turner, Lucy M	Calculated using CO2SYS
32	Calcite saturation state	Omega Cal		Turner, Lucy M	Calculated using CO2SYS
33	Calcite saturation state, standard error	Omega Cal std e	±	Turner, Lucy M	Calculated using CO2SYS
34	Aragonite saturation state	Omega Arg		Turner, Lucy M	Calculated using CO2SYS
35	Aragonite saturation state, standard error	Omega Arg std e	±	Turner, Lucy M	Calculated using CO2SYS
36	Station label	Station		Turner, Lucy M	Calculated using CO2SYS
37	Survival	Survival	%	Turner, Lucy M	Calculated using CO2SYS
38	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
39	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
40	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
41	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
43	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
44	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
45	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
46	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
47	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

930 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Event	2 Type (study)	3 Species	4 Reg spec no	5 URL ref (WoRMS Aphia ID)	6 Treat	7 ATP [nmol/mg]	8 ATP std e [±]	9 Repl [#] (ATP)	10 L-lactate [mol/mg]	11 L-lactate std e [±]	12 Repl [#] (l-lactate)	13 CA act/prot [U/g]	14 CA act std e [±]	15 Repl [#] (Carbonic anhydrase)	16 Sal	17 Sal std e [±]	18 Temp [°C]	19 T std e [±]	20 pH (NBS scale)	21 pH std e [±] (NBS scale)	22 AT [µmol/kg]	23 AT std e [±]	24 DIC [µmol/kg] (Calculated using CO2SYS)	25 DIC std e [±] (Calculated using CO2SYS)	26 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	27 pCO2water_SST_wet std e [±] (Calculated using CO2SYS)	28 [HCO3]- [µmol/kg] (Calculated using CO2SYS)	29 [HCO3]- std e [±] (Calculated using CO2SYS)	30 [CO3]2- [µmol/kg] (Calculated using CO2SYS)	31 [CO3]2- std e [±] (Calculated using CO2SYS)	32 Omega Cal (Calculated using CO2SYS)	33 Omega Cal std e [±] (Calculated using CO2SYS)	34 Omega Arg (Calculated using CO2SYS)	35 Omega Arg std e [±] (Calculated using CO2SYS)	36 Station (Calculated using CO2SYS)	37 Survival [%] (Calculated using CO2SYS)	38 CSC flag (Calculated using seacarb afte...)	39 pH (total scale, Calculated using...)	40 CO2 [µmol/kg] (Calculated using seacarb afte...)	41 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	42 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	43 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	44 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	45 DIC [µmol/kg] (Calculated using seacarb afte...)	46 Omega Arg (Calculated using seacarb afte...)	47 Omega Cal (Calculated using seacarb afte...)
St_Anna_rocks	Field	Cymodoce truncata (crustacean)	118936	marinespecies.org	Control	1.95	0.13	19	2.74	0.18	18	0.66	0.14	12	36.98	0.06	21.34	0.18	8.13	0.01	2601.33	3.21	2315.40	6.39	502.24	9.05	2088.02	8.73	211.84	2.90	5.00	0.07	3.27	0.05			26	8.01	15.30	495.10	496.76	2090.66	210.52	2316.49	3.25	4.97
St_Anna_rocks	Field	Cymodoce truncata (crustacean)	118936	marinespecies.org	acidified	1.85	0.18	15	2.18	0.14	13	0.27	0.07	11	37.04	0.06	20.23	0.10	7.29	0.04	2608.15	6.22	2733.52	24.25	6144.17	552.63	2482.30	13.96	52.00	5.08	1.22	0.12	0.80	0.08			26	7.16	139.01	4366.21	4381.10	2518.94	36.84	2694.79	0.57	0.87
San_Pietro_promontory	Field	Dynamene torelliae (crustacean)	118943	marinespecies.org	Control	1.71	0.12	35	3.08	0.18	20	0.17	0.05	25	36.98	0.06	21.34	0.18	8.13	0.01	2601.33	3.21	2315.40	6.39	502.24	9.05	2088.02	8.73	211.84	2.90	5.00	0.07	3.27	0.05			26	8.01	15.30	495.10	496.76	2090.66	210.52	2316.49	3.25	4.97
San_Pietro_promontory	Field	Dynamene torelliae (crustacean)	118943	marinespecies.org	acidified	1.46	0.13	19	2.38	0.21	14	0.43	0.12	19	37.04	0.06	20.23	0.10	7.29	0.04	2608.15	6.22	2733.52	24.25	6144.17	552.63	2482.30	13.96	52.00	5.08	1.22	0.12	0.80	0.08			26	7.16	139.01	4366.21	4381.10	2518.94	36.84	2694.79	0.57	0.87
Castello_Aragonese_south	Field	Dynamene bifida (crustacean)	118940	marinespecies.org	Control	1.60	0.55	37	2.15	0.18	22	0.15	0.02	30	36.98	0.06	21.34	0.18	8.13	0.01	2601.33	3.21	2315.40	6.39	502.24	9.05	2088.02	8.73	211.84	2.90	5.00	0.07	3.27	0.05			26	8.01	15.30	495.10	496.76	2090.66	210.52	2316.49	3.25	4.97
Castello_Aragonese_south	Field	Dynamene bifida (crustacean)	118940	marinespecies.org	acidified	1.34	0.07	30	2.51	0.40	10	0.17	0.03	15	37.04	0.06	20.23	0.10	7.29	0.04	2608.15	6.22	2733.52	24.25	6144.17	552.63	2482.30	13.96	52.00	5.08	1.22	0.12	0.80	0.08			26	7.16	139.01	4366.21	4381.10	2518.94	36.84	2694.79	0.57	0.87
St_Anna_rocks	Field	Cymodoce truncata (crustacean)	118936	marinespecies.org	contol										36.93	0.11	21.29	0.30	8.12	0.01	2612.93	4.68	2333.39	9.19	518.05	13.50	2109.27	12.82	208.07	4.54	4.91	0.11	3.21	0.07	C1	0.00	26	8.00	16.00	515.85	517.58	2114.45	205.83	2336.25	3.18	4.86
St_Anna_rocks	Field	Cymodoce truncata (crustacean)	118936	marinespecies.org	contol										37.00	0.10	21.36	0.32	8.13	0.01	2589.79	6.92	2305.76	13.71	505.30	19.00	2079.87	18.26	210.26	5.61	4.96	0.13	3.24	0.09	C2	100.00	26	8.01	15.40	497.12	498.79	2084.10	208.42	2307.88	3.21	4.92
St_Anna_rocks	Field	Cymodoce truncata (crustacean)	118936	marinespecies.org	contol										37.02	0.09	21.37	0.31	8.15	0.01	2600.38	4.05	2305.98	9.62	482.89	14.19	2073.80	13.79	217.29	4.98	5.12	0.12	3.35	0.08	C3	90.00	26	8.03	14.60	472.33	473.91	2073.44	217.31	2305.33	3.35	5.12
St_Anna_rocks	Field	Cymodoce truncata (crustacean)	118936	marinespecies.org	acidified										37.00	0.08	20.17	0.18	7.49	0.06	2620.00	4.19	2640.00	31.65	3682.36	702.65	2455.28	20.62	68.08	7.79	1.60	0.18	1.05	0.12	A4	40.00	26	7.37	81.60	2555.78	2564.48	2487.37	54.80	2623.75	0.84	1.29
St_Anna_rocks	Field	Cymodoce truncata (crustacean)	118936	marinespecies.org	acidified										37.08	0.09	20.12	0.18	7.16	0.08	2597.40	11.21	2802.89	47.34	8608.77	1150.84	2481.77	27.81	47.76	11.14	1.12	0.26	0.73	0.17	A5	70.00	26	7.04	177.72	5564.42	5583.45	2534.21	26.10	2738.06	0.40	0.62
St_Anna_rocks	Field	Cymodoce truncata (crustacean)	118936	marinespecies.org	acidified										37.05	0.12	20.41	0.16	7.21	0.06	2606.72	14.29	2759.76	41.80	6216.07	798.54	2509.83	23.58	40.02	6.50	0.94	0.15	0.61	0.10	A6	50.00	26	7.09	157.77	4978.42	4995.37	2535.22	29.60	2722.57	0.45	0.70
San_Pietro_promontory	Field	Dynamene torelliae (crustacean)	118943	marinespecies.org	contol										36.93	0.11	21.29	0.30	8.12	0.01	2612.93	4.68	2333.39	9.19	518.05	13.50	2109.27	12.82	208.07	4.54	4.91	0.11	3.21	0.07	C1	100.00	26	8.00	16.00	515.85	517.58	2114.45	205.83	2336.25	3.18	4.86
San_Pietro_promontory	Field	Dynamene torelliae (crustacean)	118943	marinespecies.org	contol										37.00	0.10	21.36	0.32	8.13	0.01	2589.79	6.92	2305.76	13.71	505.30	19.00	2079.87	18.26	210.26	5.61	4.96	0.13	3.24	0.09	C2	100.00	26	8.01	15.40	497.12	498.79	2084.10	208.42	2307.88	3.21	4.92
San_Pietro_promontory	Field	Dynamene torelliae (crustacean)	118943	marinespecies.org	contol										37.02	0.09	21.37	0.31	8.15	0.01	2600.38	4.05	2305.98	9.62	482.89	14.19	2073.80	13.79	217.29	4.98	5.12	0.12	3.35	0.08	C3	100.00	26	8.03	14.60	472.33	473.91	2073.44	217.31	2305.33	3.35	5.12
San_Pietro_promontory	Field	Dynamene torelliae (crustacean)	118943	marinespecies.org	acidified										37.00	0.08	20.17	0.18	7.49	0.06	2620.00	4.19	2640.00	31.65	3682.36	702.65	2455.28	20.62	68.08	7.79	1.60	0.18	1.05	0.12	A4	16.67	26	7.37	81.60	2555.78	2564.48	2487.37	54.80	2623.75	0.84	1.29
San_Pietro_promontory	Field	Dynamene torelliae (crustacean)	118943	marinespecies.org	acidified										37.08	0.09	20.12	0.18	7.16	0.08	2597.40	11.21	2802.89	47.34	8608.77	1150.84	2481.77	27.81	47.76	11.14	1.12	0.26	0.73	0.17	A5	83.33	26	7.04	177.72	5564.42	5583.45	2534.21	26.10	2738.06	0.40	0.62
San_Pietro_promontory	Field	Dynamene torelliae (crustacean)	118943	marinespecies.org	acidified										37.05	0.12	20.41	0.16	7.21	0.06	2606.72	14.29	2759.76	41.80	6216.07	798.54	2509.83	23.58	40.02	6.50	0.94	0.15	0.61	0.10	A6	75.00	26	7.09	157.77	4978.42	4995.37	2535.22	29.60	2722.57	0.45	0.70
Castello_Aragonese_south	Field	Dynamene bifida (crustacean)	118940	marinespecies.org	acidified										37.00	0.08	20.17	0.18	7.49	0.06	2620.00	4.19	2640.00	31.65	3682.36	702.65	2455.28	20.62	68.08	7.79	1.60	0.18	1.05	0.12	A4	73.30	26	7.37	81.60	2555.78	2564.48	2487.37	54.80	2623.75	0.84	1.29
Castello_Aragonese_south	Field	Dynamene bifida (crustacean)	118940	marinespecies.org	acidified										37.08	0.09	20.12	0.18	7.16	0.08	2597.40	11.21	2802.89	47.34	8608.77	1150.84	2481.77	27.81	47.76	11.14	1.12	0.26	0.73	0.17	A5	53.33	26	7.04	177.72	5564.42	5583.45	2534.21	26.10	2738.06	0.40	0.62
Castello_Aragonese_south	Field	Dynamene bifida (crustacean)	118940	marinespecies.org	acidified										37.05	0.12	20.41	0.16	7.21	0.06	2606.72	14.29	2759.76	41.80	6216.07	798.54	2509.83	23.58	40.02	6.50	0.94	0.15	0.61	0.10	A6	100.00	26	7.09	157.77	4978.42	4995.37	2535.22	29.60	2722.57	0.45	0.70
Castello_Aragonese_south	Field	Dynamene bifida (crustacean)	118940	marinespecies.org	contol										36.93	0.11	21.29	0.30	8.12	0.01	2612.93	4.68	2333.39	9.19	518.05	13.50	2109.27	12.82	208.07	4.54	4.91	0.11	3.21	0.07	C1	86.67	26	8.00	16.00	515.85	517.58	2114.45	205.83	2336.25	3.18	4.86
Castello_Aragonese_south	Field	Dynamene bifida (crustacean)	118940	marinespecies.org	contol										37.00	0.10	21.36	0.32	8.13	0.01	2589.79	6.92	2305.76	13.71	505.30	19.00	2079.87	18.26	210.26	5.61	4.96	0.13	3.24	0.09	C2	86.67	26	8.01	15.40	497.12	498.79	2084.10	208.42	2307.88	3.21	4.92
Castello_Aragonese_south	Field	Dynamene bifida (crustacean)	118940	marinespecies.org	contol										37.02	0.09	21.37	0.31	8.15	0.01	2600.38	4.05	2305.98	9.62	482.89	14.19	2073.80	13.79	217.29	4.98	5.12	0.12	3.35	0.08	C3	100.00	26	8.03	14.60	472.33	473.91	2073.44	217.31	2305.33	3.35	5.12