Citation:

PANGAEA.873255

Name: Biogeographic variability in the physiological response of the cold-water coral Lophelia pertusa to ocean acidification
Published: 2016
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Animalia; Behaviour; Benthic animals; Benthos; Calcification/Dissolution; Cnidaria; Containers and aquaria (20-1000 L or < 1 m**2); Deep-sea; Laboratory experiment; Lophelia pertusa; North Atlantic; Respiration; Single species; Temperate

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Abstract:

While ocean acidification is a global issue, the severity of ecosystem effects is likely to vary considerably at regional scales. The lack of understanding of how biogeographically separated populations will respond to acidification hampers our ability to predict the future of vital ecosystems. Cold-water corals are important drivers of biodiversity in ocean basins across the world and are considered one of the most vulnerable ecosystems to ocean acidification. We tested the short-term physiological response of the cold-water coral Lophelia pertusa to three pH treatments (pH = 7.9, 7.75 and 7.6) for Gulf of Mexico (USA) and Tisler Reef (Norway) populations, and found that reductions in seawater pH elicited contrasting responses. Gulf of Mexico corals exhibited reductions in net calcification, respiration and prey capture rates with decreasing pH. In contrast, Tisler Reef corals showed only slight reductions in net calcification rates under decreased pH conditions while significantly elevating respiration and capture rates. These differences are likely the result of environmental differences (depth, pH, food supply) between the two regions, invoking the potential for local adaptation or acclimatization to alter their response to global change. However, it is also possible that variations in the methodology used in the experiments contributed to the observed differences. Regardless, these results provide insights into the resilience of L. pertusa to ocean acidification as well as the potential influence of regional differences on the viability of species in future oceans.

Keyword(s):

Animalia; Behaviour; Benthic animals; Benthos; Calcification/Dissolution; Cnidaria; Containers and aquaria (20-1000 L or < 1 m**2); Deep-sea; Laboratory experiment; Lophelia pertusa; North Atlantic; Respiration; Single species; Temperate

Related to:

Georgian, Samuel E; Dupont, Sam; Kurman, Melissa; Butler, Adam; Stromberg, Susanna M; Larsson, Ann I; Cordes, Erik E (2016): Biogeographic variability in the physiological response of the cold-water coral Lophelia pertusa to ocean acidification. Marine Ecology, 37(6), 1345-1359, https://doi.org/10.1111/maec.12373

Original version:

Cordes, Erik E; Kulathinal, Robert J (2012): Project: Physiological and genetic responses of the deep-water coral, Lophelia pertusa, to ongoing ocean acidification in the Gulf of Mexico. Biological and Chemical Oceanography Data Management Office, https://www.bco-dmo.org/project/2224

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2017-03-07.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Georgian, Samuel E		study
2	Species	Species		Georgian, Samuel E
3	Registration number of species	Reg spec no		Georgian, Samuel E
4	Uniform resource locator/link to reference	URL ref		Georgian, Samuel E		WoRMS Aphia ID
5	Location	Location		Georgian, Samuel E
6	pH	pH		Georgian, Samuel E		treatment
7	Identification	ID		Georgian, Samuel E		tank
8	Identification	ID		Georgian, Samuel E		fragment_num
9	Identification	ID		Georgian, Samuel E		polyps
10	Dry mass	Dry m	g	Georgian, Samuel E		starting
11	Ash free dry mass	afdm	g	Georgian, Samuel E
12	Time in days	Time	days	Georgian, Samuel E
13	Change	Change		Georgian, Samuel E		total alkalinity
14	Calcification rate	Calc rate	%/day	Georgian, Samuel E		net
15	Buoyant mass	M buoyant	mg	Georgian, Samuel E
16	Dry mass	Dry m	g	Georgian, Samuel E		sample
17	Consumption	Consmpt		Georgian, Samuel E		total Artemia salina consumed by polyps in 1 hour
18	Consumption	Consmpt		Georgian, Samuel E		Artemia salina consumed per polyp in 1 hour
19	Consumption	Consmpt		Georgian, Samuel E		Artemia salina consumed per gTW in 1 hour
20	Respiration rate, oxygen	Resp O2	µmol/h	Georgian, Samuel E
21	Respiration rate, oxygen	Resp O2	µmol/g/h	Georgian, Samuel E
22	Oxygen	O2	µmol/kg	Georgian, Samuel E
23	Alkalinity, total	AT	µmol/kg	Georgian, Samuel E
24	Salinity	Sal		Georgian, Samuel E
25	Temperature, water	Temp	°C	Georgian, Samuel E
26	pH	pH		Georgian, Samuel E		total scale
27	Aragonite saturation state	Omega Arg		Georgian, Samuel E
28	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
29	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
30	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
31	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
32	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
33	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
34	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
35	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
36	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

3154 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Location	6 pH (treatment)	7 ID (tank)	8 ID (fragment_num)	9 ID (polyps)	10 Dry m [g] (starting)	11 afdm [g]	12 Time [days]	13 Change (total alkalinity)	14 Calc rate [%/day] (net)	15 M buoyant [mg]	16 Dry m [g] (sample)	17 Consmpt (total Artemia salina consumed...)	18 Consmpt (Artemia salina consumed per p...)	19 Consmpt (Artemia salina consumed per g...)	20 Resp O2 [µmol/h]	21 Resp O2 [µmol/g/h]	22 O2 [µmol/kg]	23 AT [µmol/kg]	24 Sal	25 Temp [°C]	26 pH (total scale)	27 Omega Arg	28 CSC flag	29 CO2 [µmol/kg]	30 fCO2water_SST_wet [µatm]	31 pCO2water_SST_wet [µatm]	32 [HCO3]- [µmol/kg]	33 [CO3]2- [µmol/kg]	34 DIC [µmol/kg]	35 Omega Arg	36 Omega Cal
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.60	A	1	10	49.52	1.90	0		-0.0068	31580	49.57	41	4.1	21.55	10.08	5.30
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.60	A	2	6	38.52	1.56	0		-0.0102	24570	38.57	20	3.33	12.81	9.95	6.38
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.60	A	3	6	22.73	0.82	0		-0.0077	14500	22.76	20	3.33	24.49	7.16	8.77
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.60	A	4	9	58.62	1.87	0		-0.0089	37390	58.69	32	3.56	17.15	9.82	5.26
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.60	A	5	14	35.15	1.45	0		-0.0149	22440	35.23	35	2.5	24.06	9.79	6.73
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.60	A	6	13	34.30	1.20	0		-0.0067	21870	34.33	32	2.46	26.66	8.84	7.36
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.60	A	7	14	79.74	3.29	0		-0.0102	50870	79.85	44	3.14	13.37	15.94	4.84
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.60	A	8	13	24.33	0.91	0		-0.0072	15520	24.36	29	2.23	31.84	9.25	10.16
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.60	A	9	12	38.54	1.49	0		-0.0048	24570	38.57	23	1.92	15.45	10.11	6.79
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.60	A	1	10	49.52	1.90	14		-0.0068	31550	49.52	20	2	10.51	4.41	2.32		2347.73	35.50	8.28	7.62	0.81	8	54.48	1175.48	1180.13	2214.31	2322.16	2322.16	0.81	1.27
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.60	A	2	6	38.52	1.56	14		-0.0102	24540	38.52	8	1.33	5.13	4.47	2.86		2347.73	35.50	8.28	7.62	0.81	8	54.48	1175.48	1180.13	2214.31	2322.16	2322.16	0.81	1.27
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.60	A	3	6	22.73	0.82	14		-0.0077	14480	22.73	2	0.33	2.45	2.70	3.30		2347.73	35.50	8.28	7.62	0.81	8	54.48	1175.48	1180.13	2214.31	2322.16	2322.16	0.81	1.27
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.60	A	4	9	58.62	1.87	14		-0.0089	37340	58.62	26	2.89	13.93	3.85	2.06		2347.73	35.50	8.28	7.62	0.81	8	54.48	1175.48	1180.13	2214.31	2322.16	2322.16	0.81	1.27
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.60	A	5	14	35.15	1.45	14		-0.0149	22390	35.15	11	0.79	7.56	2.99	2.06		2347.73	35.50	8.28	7.62	0.81	8	54.48	1175.48	1180.13	2214.31	2322.16	2322.16	0.81	1.27
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.60	A	6	13	34.30	1.20	14		-0.0067	21850	34.30	17	1.31	14.17	3.52	2.93		2347.73	35.50	8.28	7.62	0.81	8	54.48	1175.48	1180.13	2214.31	2322.16	2322.16	0.81	1.27
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.60	A	7	14	79.74	3.29	14		-0.0102	50790	79.74	23	1.64	6.99	7.89	2.40		2347.73	35.50	8.28	7.62	0.81	8	54.48	1175.48	1180.13	2214.31	2322.16	2322.16	0.81	1.27
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.60	A	8	13	24.33	0.91	14		-0.0072	15500	24.33	2	0.15	2.2	2.82	3.10		2347.73	35.50	8.28	7.62	0.81	8	54.48	1175.48	1180.13	2214.31	2322.16	2322.16	0.81	1.27
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.60	A	9	12	38.54	1.49	14		-0.0048	24550	38.54	11	0.92	7.39	3.54	2.38		2347.73	35.50	8.28	7.62	0.81	8	54.48	1175.48	1180.13	2214.31	2322.16	2322.16	0.81	1.27
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.75	C	1	14	41.44	1.72	0		0.0346	26270	41.24	24	1.71	13.98	9.42	5.49
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.75	C	2	14	77.66	2.99	0		0.0378	49210	77.25	33	2.36	11.05	16.53	5.54
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.75	C	3	5	13.39	0.88	0		0.0073	8520	13.38	21	4.2	23.73	7.45	8.41
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.75	C	4	12	36.17	1.32	0		0.0203	22980	36.07	24	2	18.23	7.95	6.04
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.75	C	5	5	23.10	1.09	0		0.0094	14690	23.07	21	4.2	19.29	8.84	8.12
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.75	C	6	13	39.02	1.89	0		0.0177	24790	38.92	30	2.31	15.86	10.32	5.45
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.75	C	7	9	19.85	0.96	0		0.0156	12620	19.80	18	2	18.77	8.06	8.40
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.75	C	8	8	14.04	0.97	0		0.0072	8930	14.02	24	3	24.74	8.75	9.02
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.75	C	9	7	32.22	1.67	0		0.0085	20500	32.18	24	3.43	14.37	9.48	5.68
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.75	C	1	14	41.44	1.72	14		0.0346	26400	41.44	15	1.07	8.74	7.62	4.44		2347.68	35.00	8.25	7.95	1.61	8	24.09	517.71	519.76	2083.32	2213.42	2213.42	1.60	2.53
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.75	C	2	14	77.66	2.99	14		0.0378	49470	77.66	24	1.71	8.04	9.76	3.27		2347.68	35.00	8.25	7.95	1.61	8	24.09	517.71	519.76	2083.32	2213.42	2213.42	1.60	2.53
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.75	C	3	5	13.39	0.88	14		0.0073	8530	13.39	12	2.4	13.56	3.94	4.45		2347.68	35.00	8.25	7.95	1.61	8	24.09	517.71	519.76	2083.32	2213.42	2213.42	1.60	2.53
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.75	C	4	12	36.17	1.32	14		0.0203	23040	36.17	18	1.5	13.67	4.60	3.49		2347.68	35.00	8.25	7.95	1.61	8	24.09	517.71	519.76	2083.32	2213.42	2213.42	1.60	2.53
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.75	C	5	5	23.10	1.09	14		0.0094	14710	23.10	12	2.4	11.02	4.31	3.96		2347.68	35.00	8.25	7.95	1.61	8	24.09	517.71	519.76	2083.32	2213.42	2213.42	1.60	2.53
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.75	C	6	13	39.02	1.89	14		0.0177	24860	39.02	21	1.62	11.1	8.73	4.62		2347.68	35.00	8.25	7.95	1.61	8	24.09	517.71	519.76	2083.32	2213.42	2213.42	1.60	2.53
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.75	C	7	9	19.85	0.96	14		0.0156	12640	19.85	12	1.33	12.51	6.60	6.88		2347.68	35.00	8.25	7.95	1.61	8	24.09	517.71	519.76	2083.32	2213.42	2213.42	1.60	2.53
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.75	C	8	8	14.04	0.97	14		0.0072	8940	14.04	15	1.88	15.46	5.10	5.25		2347.68	35.00	8.25	7.95	1.61	8	24.09	517.71	519.76	2083.32	2213.42	2213.42	1.60	2.53
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.75	C	9	7	32.22	1.67	14		0.0085	20520	32.22	9	1.29	5.39	5.67	3.39		2347.68	35.00	8.25	7.95	1.61	8	24.09	517.71	519.76	2083.32	2213.42	2213.42	1.60	2.53
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.90	B	1	9	78.13	2.86	0		0.0387	49400	77.71	38	4.22	13.27	14.91	5.21
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.90	B	2	15	77.84	3.00	0		0.0293	49280	77.52	35	2.33	11.66	17.33	5.77
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.90	B	3	13	36.42	1.41	0		0.0262	23070	36.28	23	1.77	16.27	10.41	7.37
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.90	B	4	12	52.71	2.13	0		0.0377	33330	52.43	26	2.17	12.21	12.09	5.68
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.90	B	5	10	13.63	0.55	0		0.0371	8620	13.56	14	1.4	25.66	4.97	9.10
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.90	B	6	9	9.07	0.83	0		0.0359	5730	9.02	26	2.89	31.23	8.64	10.38
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.90	B	7	15	25.64	0.89	0		0.0484	16190	25.47	26	1.73	29.24	8.89	10.00
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.90	B	8	15	16.68	0.46	0		0.0331	10550	16.60	17	1.13	37.28	3.92	8.59
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.90	B	9	10	31.88	1.32	0		0.0408	20150	31.70	26	2.6	19.75	9.44	7.17
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.90	B	1	9	78.13	2.86	14		0.0387	49670	78.13	45	5	15.72	13.79	4.82		2316.31	35.00	8.13	7.71	0.95	8	43.52	931.46	935.15	2158.86	2265.29	2265.29	0.95	1.50
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.90	B	2	15	77.84	3.00	14		0.0293	49490	77.84	30	2	9.99	16.27	5.42		2316.31	35.00	8.13	7.71	0.95	8	43.52	931.46	935.15	2158.86	2265.29	2265.29	0.95	1.50
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.90	B	3	13	36.42	1.41	14		0.0262	23150	36.42	18	1.38	12.73	7.57	5.36		2316.31	35.00	8.13	7.71	0.95	8	43.52	931.46	935.15	2158.86	2265.29	2265.29	0.95	1.50
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.90	B	4	12	52.71	2.13	14		0.0377	33510	52.71	24	2	11.28	12.77	6.00		2316.31	35.00	8.13	7.71	0.95	8	43.52	931.46	935.15	2158.86	2265.29	2265.29	0.95	1.50
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.90	B	5	10	13.63	0.55	14		0.0371	8660	13.63	24	2.4	43.99	5.18	9.50		2316.31	35.00	8.13	7.71	0.95	8	43.52	931.46	935.15	2158.86	2265.29	2265.29	0.95	1.50
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.90	B	6	9	9.07	0.83	14		0.0359	5760	9.07	15	1.67	18.02	6.12	7.35		2316.31	35.00	8.13	7.71	0.95	8	43.52	931.46	935.15	2158.86	2265.29	2265.29	0.95	1.50
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.90	B	7	15	25.64	0.89	14		0.0484	16300	25.64	24	1.6	26.99	7.61	8.56		2316.31	35.00	8.13	7.71	0.95	8	43.52	931.46	935.15	2158.86	2265.29	2265.29	0.95	1.50
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.90	B	8	15	16.68	0.46	14		0.0331	10600	16.68	12	0.8	26.32	2.59	5.69		2316.31	35.00	8.13	7.71	0.95	8	43.52	931.46	935.15	2158.86	2265.29	2265.29	0.95	1.50
laboratory	Lophelia pertusa	135161	marinespecies.org	Gulf_of_Mexico	7.90	B	9	10	31.88	1.32	14		0.0408	20270	31.88	24	2.4	18.23	12.12	9.21		2316.31	35.00	8.13	7.71	0.95	8	43.52	931.46	935.15	2158.86	2265.29	2265.29	0.95	1.50
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.60	5	a	6	21.83	1.48	0	470.5	0.0652			66	10.33	41.95	8.09	5.47
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.60	5	b	4	24.09	0.59	0	360.5	0.0641			34	7.5	51.11	3.55	6.05
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.60	5	c	3	7.08	0.46	0	436.0	0.0746			29	8.33	54.36	3.62	7.88
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.60	5	d	3	8.92	0.38	0	373.0	0.0592			27	7.67	59.98	2.62	6.84
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.60	5	a	6	21.83	1.48	14	460.0	0.0638			81	12.67	51.43	11.16	7.56	240.8	2319.80	33.00	7.88	7.64	0.66	8	53.17	1115.28	1119.71	2192.16	2296.70	2296.70	0.78	1.24
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.60	5	b	4	24.09	0.59	14	385.5	0.0686			59	13.5	92	4.79	8.16	240.8	2319.80	33.00	7.88	7.64	0.66	8	53.17	1115.28	1119.71	2192.16	2296.70	2296.70	0.78	1.24
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.60	5	c	3	7.08	0.46	14	412.5	0.0706			53	16	104.37	4.37	9.50	240.8	2319.80	33.00	7.88	7.64	0.66	8	53.17	1115.28	1119.71	2192.16	2296.70	2296.70	0.78	1.24
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.60	5	d	3	8.92	0.38	14	296.5	0.0470			49	14.67	114.74	4.07	10.61	240.8	2319.80	33.00	7.88	7.64	0.66	8	53.17	1115.28	1119.71	2192.16	2296.70	2296.70	0.78	1.24
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.60	7	a	7	18.93	0.82	0	566.0	0.0521			42	5.43	46.58	4.31	5.28
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.60	7	b	6	21.97	1.59	0	513.5	0.0664			69	10.83	40.98	7.24	4.56
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.60	7	c	5	8.88	0.25	0	1052.0	0.0539			47	8.6	171.95	1.56	6.23
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.60	7	d	5	9.87	0.60	0	279.0	0.0802			53	9.8	81.77	3.58	5.97
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.60	7	a	7	18.93	0.82	14	549.5	0.0506			83	11.14	95.61	6.72	8.24	219.4	2307.82	33.80	7.98	7.64	0.78	8	52.30	1105.98	1110.37	2177.58	2282.09	2282.09	0.79	1.25
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.60	7	b	6	21.97	1.59	14	484.0	0.0626			75	11.67	44.14	10.43	6.57	219.4	2307.82	33.80	7.98	7.64	0.78	8	52.30	1105.98	1110.37	2177.58	2282.09	2282.09	0.79	1.25
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.60	7	c	5	8.88	0.25	14	503.0	0.0258			38	6.6	131.96	2.96	11.85	219.4	2307.82	33.80	7.98	7.64	0.78	8	52.30	1105.98	1110.37	2177.58	2282.09	2282.09	0.79	1.25
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.60	7	d	5	9.87	0.60	14	204.0	0.0586			55	10	83.43	5.34	8.91	219.4	2307.82	33.80	7.98	7.64	0.78	8	52.30	1105.98	1110.37	2177.58	2282.09	2282.09	0.79	1.25
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.75	2	a	5	15.89	0.88	0	504.5	0.0509			39	7.4	42.23	4.94	5.64
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.75	2	b	4	12.38	0.35	0	541.0	0.0494			38	9	103.46	1.80	5.17
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.75	2	c	8	12.87	0.49	0	271.0	0.0843			39	4.63	75.96	2.84	5.84
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.75	2	d	3	13.88	0.78	0	338.0	0.0835			31	9.67	37.42	3.10	4.00
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.75	2	a	5	15.89	0.88	14	614.0	0.0619			62	12.2	69.63	5.76	6.57	240.1	2237.98	7.75	32.60	7.77	1.00	8	32.75	1208.45	1211.98	2073.53	2183.05	2183.05	1.46	2.50
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.75	2	b	4	12.38	0.35	14	563.5	0.0515			41	10	114.95	3.02	8.67	240.1	2237.98	7.75	32.60	7.77	1.00	8	32.75	1208.45	1211.98	2073.53	2183.05	2183.05	1.46	2.50
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.75	2	c	8	12.87	0.49	14	113.5	0.0353			66	8.13	133.45	3.66	7.51	240.1	2237.98	7.75	32.60	7.77	1.00	8	32.75	1208.45	1211.98	2073.53	2183.05	2183.05	1.46	2.50
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.75	2	d	3	13.88	0.78	14	214.0	0.0528			39	12.67	49.03	5.04	6.50	240.1	2237.98	7.75	32.60	7.77	1.00	8	32.75	1208.45	1211.98	2073.53	2183.05	2183.05	1.46	2.50
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.75	6	a	5	23.92	0.50	0	537.0	0.0625			45	8.6	86.14	2.29	4.58
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.75	6	b	5	17.02	0.96	0	672.0	0.0673			51	9.8	50.8	5.53	5.73
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.75	6	c	9	42.97	2.82	0	269.0	0.0667			76	8.22	26.25	14.21	5.04
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.75	6	d	6	7.66	0.34	0	335.0	0.0747			45	7.17	125.19	2.81	8.18
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.75	6	a	5	23.92	0.50	14	312.0	0.0363			56	11	110.18	3.10	6.22	237.3	2310.82	7.75	33.70	7.74	1.02	8	35.67	1349.77	1353.67	2146.00	2258.77	2258.77	1.49	2.54
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.75	6	b	5	17.02	0.96	14	626.0	0.0627			63	12.4	64.28	6.59	6.83	237.3	2310.82	7.75	33.70	7.74	1.02	8	35.67	1349.77	1353.67	2146.00	2258.77	2258.77	1.49	2.54
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.75	6	c	9	42.97	2.82	14	167.0	0.0414			90	9.89	31.57	17.46	6.19	237.3	2310.82	7.75	33.70	7.74	1.02	8	35.67	1349.77	1353.67	2146.00	2258.77	2258.77	1.49	2.54
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.75	6	d	6	7.66	0.34	14	202.5	0.0452			42	6.83	119.37	3.67	10.69	237.3	2310.82	7.75	33.70	7.74	1.02	8	35.67	1349.77	1353.67	2146.00	2258.77	2258.77	1.49	2.54
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.90	1	a	6	29.82	0.62	0	699.0	0.0516			56	8.33	80.6	2.64	4.26
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.90	1	b	4	10.87	0.47	0	344.5	0.0698			44	9.5	80.82	3.28	6.98
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.90	1	c	6	14.02	0.75	0	494.5	0.0777			52	7.67	61.74	4.95	6.64
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.90	1	d	9	35.91	2.87	0	832.0	0.0510			79	8.11	25.47	19.07	6.66
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.90	1	a	6	29.82	0.62	14	664.7	0.0491			49	7.83	75.76	3.08	4.97	231.9	2324.78	32.60	7.98	7.88	1.36	8	29.52	619.85	622.32	2112.80	2227.87	2227.87	1.30	2.07
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.90	1	b	4	10.87	0.47	14	419.7	0.0850			43	10.25	87.2	3.30	7.02	231.9	2324.78	32.60	7.98	7.88	1.36	8	29.52	619.85	622.32	2112.80	2227.87	2227.87	1.30	2.07
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.90	1	c	6	14.02	0.75	14	508.7	0.0799			49	7.83	63.08	4.96	6.66	231.9	2324.78	32.60	7.98	7.88	1.36	8	29.52	619.85	622.32	2112.80	2227.87	2227.87	1.30	2.07
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.90	1	d	9	35.91	2.87	14	772.2	0.0473			64	6.89	21.64	11.51	4.02	231.9	2324.78	32.60	7.98	7.88	1.36	8	29.52	619.85	622.32	2112.80	2227.87	2227.87	1.30	2.07
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.90	4	a	6	31.09	1.33	0	720.5	0.0510			68	10.33	46.46	7.64	5.73
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.90	4	b	5	14.99	0.70	0	499.0	0.0733			49	8.6	61.51	3.47	4.97
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.90	4	c	7	17.62	0.69	0	616.5	0.0771			44	5.43	55.4	3.21	4.69
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.90	4	d	4	12.89	0.49	0	460.0	0.0786			47	10.25	83.81	2.62	5.36
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.90	4	a	6	31.09	1.33	14	772.2	0.0547			60	9.67	43.46	7.81	5.85	238.4	2302.83	33.30	7.88	7.86	1.41	8	30.58	642.60	645.16	2098.21	2210.98	2210.98	1.25	1.98
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.90	4	b	5	14.99	0.70	14	516.2	0.0758			49	9.4	67.23	3.28	4.69	238.4	2302.83	33.30	7.88	7.86	1.41	8	30.58	642.60	645.16	2098.21	2210.98	2210.98	1.25	1.98
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.90	4	c	7	17.62	0.69	14	494.2	0.0618			49	6.71	68.52	3.28	4.78	238.4	2302.83	33.30	7.88	7.86	1.41	8	30.58	642.60	645.16	2098.21	2210.98	2210.98	1.25	1.98
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	7.90	4	d	4	12.89	0.49	14	492.7	0.0842			43	10.25	83.81	2.78	5.68	238.4	2302.83	33.30	7.88	7.86	1.41	8	30.58	642.60	645.16	2098.21	2210.98	2210.98	1.25	1.98
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	8.10	3	a	7	35.99	0.99	0	763.7	0.0467			54	6.43	45.59	5.89	5.97
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	8.10	3	b	6	25.98	1.27	0	705.2	0.0598			65	9.33	44.13	5.32	4.19
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	8.10	3	c	5	19.98	0.62	0	664.2	0.0732			45	7.2	57.96	4.57	7.36
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	8.10	3	d	7	18.71	0.97	0	679.7	0.0800			51	6	43.27	5.84	6.02
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	8.10	3	a	7	35.99	0.99	14	727.7	0.0445			58	8.29	58.77	5.70	5.77	246.5	2326.78	32.60	7.98	8.10	2.31	8	16.79	352.68	354.08	1995.03	2145.90	2145.90	2.04	3.24
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	8.10	3	b	6	25.98	1.27	14	723.7	0.0613			51	8.5	40.19	4.92	3.88	246.5	2326.78	32.60	7.98	8.10	2.31	8	16.79	352.68	354.08	1995.03	2145.90	2145.90	2.04	3.24
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	8.10	3	c	5	19.98	0.62	14	713.2	0.0786			45	9	72.45	4.29	6.91	246.5	2326.78	32.60	7.98	8.10	2.31	8	16.79	352.68	354.08	1995.03	2145.90	2145.90	2.04	3.24
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	8.10	3	d	7	18.71	0.97	14	739.7	0.0870			58	8.29	59.75	5.40	5.56	246.5	2326.78	32.60	7.98	8.10	2.31	8	16.79	352.68	354.08	1995.03	2145.90	2145.90	2.04	3.24
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	8.10	8	a	5	16.87	0.42	0	567.2	0.0740			52	8.6	102.17	2.33	5.53
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	8.10	8	b	5	12.98	0.38	0	441.2	0.0748			57	9.6	127.77	2.94	7.83
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	8.10	8	c	5	14.98	0.66	0	556.2	0.0817			47	7.6	57.22	3.27	4.92
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	8.10	8	d	6	17.11	0.52	0	610.7	0.0786			56	7.83	91.05	2.88	5.58
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	8.10	8	a	5	16.87	0.42	14	622.2	0.0812			30	6	71.28	2.43	5.78	242.1	2310.50	33.20	7.78	8.12	2.14	8	15.79	330.53	331.85	1964.80	2119.75	2119.75	2.12	3.35
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	8.10	8	b	5	12.98	0.38	14	398.2	0.0675			37	7.4	98.49	3.03	8.06	242.1	2310.50	33.20	7.78	8.12	2.14	8	15.79	330.53	331.85	1964.80	2119.75	2119.75	2.12	3.35
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	8.10	8	c	5	14.98	0.66	14	539.2	0.0792			48	9.6	72.28	3.08	4.64	242.1	2310.50	33.20	7.78	8.12	2.14	8	15.79	330.53	331.85	1964.80	2119.75	2119.75	2.12	3.35
laboratory	Lophelia pertusa	135161	marinespecies.org	Tisler_Reef	8.10	8	d	6	17.11	0.52	14	642.2	0.0826			34	5.67	65.87	2.38	4.62	242.1	2310.50	33.20	7.78	8.12	2.14	8	15.79	330.53	331.85	1964.80	2119.75	2119.75	2.12	3.35