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Roth, Olivia; Landis, Susanne H (2017): Trans-generational plasticity: Gene expression via Fluidigm chip system on pipefish Syngnathus typhle. PANGAEA, https://doi.org/10.1594/PANGAEA.872606, Supplement to: Roth, O; Landis, SH (2017): Trans-generational plasticity in response to immune challenge is constrained by heat stress. Evolutionary Applications, https://doi.org/10.1111/eva.12473

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Abstract:
Trans-generational plasticity is the adjustment of phenotypes to changing habitat conditions that persist longer than the individual lifetime. Fitness benefits (adaptive TGP) are expected upon matching parent-offspring environments. In a global change scenario, several performance-related environmental factors are changing simultaneously. This lowers the predictability of offspring environmental conditions, potentially hampering the benefits of trans-generational plasticity. For the first time, we here explore how the combination of an abiotic and a biotic environmental factor in the parental generation plays out as trans-generational effect in the offspring.
We fully reciprocally exposed the parental generation of the pipefish Syngnathus typhle to an immune challenge and elevated temperatures simulating a naturally occurring heatwave. Upon mating and male pregnancy, offspring were kept in ambient or elevated temperature regimes combined with a heat-killed bacterial epitope treatment. Differential gene expression (immune genes and DNA- and histone-modification genes) suggests that the combined change of an abiotic and a biotic factor in the parental generation had interactive effects on offspring performance, the temperature effect dominated over the immune challenge impact. The benefits of certain parental environmental conditions on offspring performance did not sum up when abiotic and biotic factors were changed simultaneously supporting that available resources are limited that can be allocated to phenotypic trans-generational effects. Temperature is the master regulator of trans-generational phenotypic plasticity, which potentially implies a conflict in the allocation of resources towards several environmental factors. This asks for a reassessment of trans-generational plasticity as a short-term option to buffer environmental variation in the light of climate change.
Comment:
delta ct values of gene expression assessed in Fluidigm qPCR
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1SpeciesSpeciesRoth, Olivia
2Sample code/labelSample labelRoth, Oliviafluidigm chip run (gene expression)
3IdentificationIDRoth, Olivia
4ReplicateReplicateRoth, Olivia
5Body lengthBLmmRoth, Oliviasize of the offspring
6FamilyFamilyRoth, Oliviabreeding pair/ parents
7TreatmentTreatRoth, Oliviaparental treatment combined
8TreatmentTreatRoth, Oliviatemperature treatment parents: H=hot, C=cold
9TreatmentTreatRoth, OliviaVibrio treatment parents: V=Vibrio, N=Naive
10TreatmentTreatRoth, Oliviatreatment offspring combined
11TreatmentTreatRoth, Oliviatemperature treatment offspring: H=hot, C=cold
12TreatmentTreatRoth, OliviaVibrio treatment offspring: V=Vibrio, N=Naive
13TreatmentTreatRoth, Oliviaparental treatment combined with temperature offspring treatment
14TreatmentTreatRoth, Oliviaparental and offspring treatment combined
15Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaLymphocyte antigen 75
16Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaHuman immunodeficiency virus enhancer prot2
17Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaHuman immunodeficiency virus enhancer prot3
18Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaCD45 (leukocyte common antigen)
19Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaIntegrin-beta 1
20Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaImmunoglobulin light chain
21Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaLectin potein type II
22Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaLectin protein type I
23Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaCoagulation factor II
24Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaHeat shock protein 60
25Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaIk cytokine(RED-protein)
26Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaInterleukin 10
27Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaKinesin
28Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaNatural resistance-assoc macrophage protein
29Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaAllograft inflammation factor
30Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaTranslocator protein
31Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaLPS-induced TNF-_ factor (LITAF)
32Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaTransferrin
33Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaCalreticulin
34Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaInterferon induced transmembrane protein 3
35Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaInterleukin 8
36Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaTap-binding protein (Tapasin)
37Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaTyroproteinkinase
38Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaChemokine 7
39Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaLysine-specific-demethylase 5B
40Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaLysine-specific-histone demethylase NO66
41Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaLysine-specific demethylase 6A
42Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaDNA-Methyltransferase 1
43Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaDNA-Methyltransferase 3a
44Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaDNA-Methyltransferase 3b
45Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaN(6)-adenine-specific DNA methyltransferase 2
46Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaHistone methyltransferase
47Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaLymphocyte cytosolic protein 2
48Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaHistone-cacetyltransferase (KAT2)
49Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaComplement component 1q
50Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaComplement component 3
51Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaComplement component 9
52Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaDDP-glycosyltransferase
53Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaGlucose 6-phosphate dehydrogenase (G6PD)
54Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaHistone deacetylase 1-like
55Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaHistone deacetylase 3-like
56Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaHistone deacetylase 6-like
57Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaHemK-methyltransferase family member 2
58Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaLymphocyte cytosolic protein 2
59Threshold cycle, quantitative polymerase chain reactionCt qPCRRoth, OliviaHistone-acetyltransferase (HAT1)acetyltransferase Histone-acetyltransferase (HAT1)
Size:
17228 data points

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