Citation:

eva.12473

Name: Trans-generational plasticity: Gene expression via Fluidigm chip system on pipefish Syngnathus typhle
Published: 2017-02-22
License: https://creativecommons.org/licenses/by/3.0/

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Published: 2017-02-22 • DOI registered: 2017-03-27

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Abstract:

Trans-generational plasticity is the adjustment of phenotypes to changing habitat conditions that persist longer than the individual lifetime. Fitness benefits (adaptive TGP) are expected upon matching parent-offspring environments. In a global change scenario, several performance-related environmental factors are changing simultaneously. This lowers the predictability of offspring environmental conditions, potentially hampering the benefits of trans-generational plasticity. For the first time, we here explore how the combination of an abiotic and a biotic environmental factor in the parental generation plays out as trans-generational effect in the offspring.

We fully reciprocally exposed the parental generation of the pipefish Syngnathus typhle to an immune challenge and elevated temperatures simulating a naturally occurring heatwave. Upon mating and male pregnancy, offspring were kept in ambient or elevated temperature regimes combined with a heat-killed bacterial epitope treatment. Differential gene expression (immune genes and DNA- and histone-modification genes) suggests that the combined change of an abiotic and a biotic factor in the parental generation had interactive effects on offspring performance, the temperature effect dominated over the immune challenge impact. The benefits of certain parental environmental conditions on offspring performance did not sum up when abiotic and biotic factors were changed simultaneously supporting that available resources are limited that can be allocated to phenotypic trans-generational effects. Temperature is the master regulator of trans-generational phenotypic plasticity, which potentially implies a conflict in the allocation of resources towards several environmental factors. This asks for a reassessment of trans-generational plasticity as a short-term option to buffer environmental variation in the light of climate change.

Comment:

delta ct values of gene expression assessed in Fluidigm qPCR

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Comment
1	Species	Species		Roth, Olivia
2	Sample code/label	Sample label		Roth, Olivia	fluidigm chip run (gene expression)
3	Identification	ID		Roth, Olivia
4	Replicate	Repl		Roth, Olivia
5	Body length	BL	mm	Roth, Olivia	size of the offspring
6	Family	Family		Roth, Olivia	breeding pair/ parents
7	Treatment	Treat		Roth, Olivia	parental treatment combined
8	Treatment	Treat		Roth, Olivia	temperature treatment parents: H=hot, C=cold
9	Treatment	Treat		Roth, Olivia	Vibrio treatment parents: V=Vibrio, N=Naive
10	Treatment	Treat		Roth, Olivia	treatment offspring combined
11	Treatment	Treat		Roth, Olivia	temperature treatment offspring: H=hot, C=cold
12	Treatment	Treat		Roth, Olivia	Vibrio treatment offspring: V=Vibrio, N=Naive
13	Treatment	Treat		Roth, Olivia	parental treatment combined with temperature offspring treatment
14	Treatment	Treat		Roth, Olivia	parental and offspring treatment combined
15	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Lymphocyte antigen 75
16	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Human immunodeficiency virus enhancer prot2
17	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Human immunodeficiency virus enhancer prot3
18	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	CD45 (leukocyte common antigen)
19	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Integrin-beta 1
20	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Immunoglobulin light chain
21	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Lectin potein type II
22	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Lectin protein type I
23	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Coagulation factor II
24	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Heat shock protein 60
25	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Ik cytokine(RED-protein)
26	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Interleukin 10
27	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Kinesin
28	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Natural resistance-assoc macrophage protein
29	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Allograft inflammation factor
30	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Translocator protein
31	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	LPS-induced TNF-_ factor (LITAF)
32	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Transferrin
33	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Calreticulin
34	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Interferon induced transmembrane protein 3
35	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Interleukin 8
36	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Tap-binding protein (Tapasin)
37	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Tyroproteinkinase
38	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Chemokine 7
39	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Lysine-specific-demethylase 5B
40	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Lysine-specific-histone demethylase NO66
41	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Lysine-specific demethylase 6A
42	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	DNA-Methyltransferase 1
43	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	DNA-Methyltransferase 3a
44	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	DNA-Methyltransferase 3b
45	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	N(6)-adenine-specific DNA methyltransferase 2
46	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Histone methyltransferase
47	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Lymphocyte cytosolic protein 2
48	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Histone-cacetyltransferase (KAT2)
49	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Complement component 1q
50	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Complement component 3
51	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Complement component 9
52	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	DDP-glycosyltransferase
53	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Glucose 6-phosphate dehydrogenase (G6PD)
54	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Histone deacetylase 1-like
55	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Histone deacetylase 3-like
56	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Histone deacetylase 6-like
57	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	HemK-methyltransferase family member 2
58	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Lymphocyte cytosolic protein 2
59	Threshold cycle, quantitative polymerase chain reaction	Ct qPCR		Roth, Olivia	Histone-acetyltransferase (HAT1)acetyltransferase Histone-acetyltransferase (HAT1)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Size:

17228 data points

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