Citation:

j.marpolbul.2016.05.001

Name: Effects of short-term hypoxia and seawater acidification on hemocyte responses of the mussel Mytilus coruscus
Published: 2016
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Animalia; Benthic animals; Benthos; Brackish waters; Containers and aquaria (20-1000 L or < 1 m**2); Laboratory experiment; Mollusca; Mytilus coruscus; North Pacific; Other metabolic rates; Oxygen; Single species; Temperate

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Abstract:

Hypoxia often intensifies with rising dissolved CO2, but the concurrent effects of hypoxia and acidification on bivalves are largely unknown. In this study, immune responses of hemocytes in the mussel Mytilus coruscus were examined under six combinations of pH (7.3, 7.7 and 8.1) and dissolved oxygen (DO) concentrations (2 mg/ L, 6 mg/L) for 72 h. Generally, total hemocyte account, phagocytosis, esterase and lysosomal content were reduced under low DO and pH conditions, whereas hemocyte mortality and reactive oxygen species production increased under low DO and pH. Both hypoxia and low pH have negative effects on mussels, but the effects of pH are not as strong as DO. Moreover, significant interactions between DO and pH occurred. However, acidification generally doesn't aggravate the effects induced by hypoxia. Acidification and hypoxia may increase disease risk and impact the aquaculture of this species.

Keyword(s):

Animalia; Benthic animals; Benthos; Brackish waters; Containers and aquaria (20-1000 L or < 1 m**2); Laboratory experiment; Mollusca; Mytilus coruscus; North Pacific; Other metabolic rates; Oxygen; Single species; Temperate

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: 30.550260 * Longitude: 121.833270

Date/Time Start: 2015-04-01T00:00:00 * Date/Time End: 2015-04-30T00:00:00

Event(s):

Shengsi_island_OA * Latitude: 30.550260 * Longitude: 121.833270 * Date/Time Start: 2015-04-01T00:00:00 * Date/Time End: 2015-04-30T00:00:00 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2017-02-16.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Wang, Youji		study
2	Species	Species		Wang, Youji
3	Registration number of species	Reg spec no		Wang, Youji
4	Uniform resource locator/link to reference	URL ref		Wang, Youji		WoRMS Aphia ID
5	Time in hours	Time	h	Wang, Youji
6	Oxygen	O2	µmol/l	Wang, Youji		treatment
7	pH	pH		Wang, Youji		treatment, NBS scale
8	Hemocyte mortality	Hemocyte mortality	%	Wang, Youji
9	Percentage	Perc	%	Wang, Youji		Granulocytes
10	Percentage	Perc	%	Wang, Youji		Hyalinocytes
11	Percentage	Perc	%	Wang, Youji		Blast-like cells
12	Percentage	Perc	%	Wang, Youji		Phagocytosis
13	Percentage	Perc	%	Wang, Youji		Esterase
14	Reactive oxygen species production	ROS prod		Wang, Youji
15	Lysosomal content	Lys		Wang, Youji
16	Hemocyte count	Hemocyte	#	Wang, Youji		total
17	Temperature, water	Temp	°C	Wang, Youji
18	Oxygen	O2	µmol/l	Wang, Youji
19	Oxygen, standard deviation	O2 std dev	±	Wang, Youji
20	Salinity	Sal		Wang, Youji
21	Salinity, standard deviation	Sal std dev	±	Wang, Youji
22	pH	pH		Wang, Youji	Potentiometric	NBS scale
23	pH, standard deviation	pH std dev	±	Wang, Youji	Potentiometric	NBS scale
24	Alkalinity, total	AT	µmol/kg	Wang, Youji	Potentiometric titration
25	Alkalinity, total, standard deviation	AT std dev	±	Wang, Youji	Potentiometric titration
26	Carbon, inorganic, dissolved	DIC	µmol/kg	Wang, Youji	Calculated using CO2SYS
27	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Wang, Youji	Calculated using CO2SYS
28	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Wang, Youji	Calculated using CO2SYS
29	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Wang, Youji	Calculated using CO2SYS
30	Calcite saturation state	Omega Cal		Wang, Youji	Calculated using CO2SYS
31	Calcite saturation state, standard deviation	Omega Cal std dev	±	Wang, Youji	Calculated using CO2SYS
32	Aragonite saturation state	Omega Arg		Wang, Youji	Calculated using CO2SYS
33	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Wang, Youji	Calculated using CO2SYS
34	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
35	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
36	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
37	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
38	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
39	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
40	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
41	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
43	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

3096 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Time [h]	6 O2 [µmol/l] (treatment)	7 pH (treatment, NBS scale)	8 Hemocyte mortality [%]	9 Perc [%] (Granulocytes)	10 Perc [%] (Hyalinocytes)	11 Perc [%] (Blast-like cells)	12 Perc [%] (Phagocytosis)	13 Perc [%] (Esterase)	14 ROS prod	15 Lys	16 Hemocyte [#] (total)	17 Temp [°C]	18 O2 [µmol/l]	19 O2 std dev [±]	20 Sal	21 Sal std dev [±]	22 pH (NBS scale, Potentiometric)	23 pH std dev [±] (NBS scale, Potentiometric)	24 AT [µmol/kg] (Potentiometric titration)	25 AT std dev [±] (Potentiometric titration)	26 DIC [µmol/kg] (Calculated using CO2SYS)	27 DIC std dev [±] (Calculated using CO2SYS)	28 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	29 pCO2 std dev [±] (Calculated using CO2SYS)	30 Omega Cal (Calculated using CO2SYS)	31 Omega Cal std dev [±] (Calculated using CO2SYS)	32 Omega Arg (Calculated using CO2SYS)	33 Omega Arg std dev [±] (Calculated using CO2SYS)	34 CSC flag (Calculated using seacarb afte...)	35 pH (total scale, Calculated using...)	36 CO2 [µmol/kg] (Calculated using seacarb afte...)	37 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	38 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	39 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	40 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	41 DIC [µmol/kg] (Calculated using seacarb afte...)	42 Omega Arg (Calculated using seacarb afte...)	43 Omega Cal (Calculated using seacarb afte...)
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	12	187.5	7.3	0.76	70.0	20.2	9.04	33.5	45.2	11.2	38.3	414375.3	20	185.6250	2.8125	25.0	0.20	7.32	0.01	2344	39	2390	37	2710	50	0.87	0.02	0.55	0.01	26	7.18	130.06	3802.07	3815.06	2286.75	24.25	2441.07	0.39	0.62
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	12	187.5	7.3	0.80	70.1	20.3	8.80	29.6	45.5	3.3	34.6	408776.2	20	185.6250	2.8125	25.0	0.20	7.32	0.01	2344	39	2390	37	2710	50	0.87	0.02	0.55	0.01	26	7.18	130.06	3802.07	3815.06	2286.75	24.25	2441.07	0.39	0.62
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	12	187.5	7.3	0.82	70.3	20.6	8.28	34.0	48.2	9.0	39.1	401765.8	20	185.6250	2.8125	25.0	0.20	7.32	0.01	2344	39	2390	37	2710	50	0.87	0.02	0.55	0.01	26	7.18	130.06	3802.07	3815.06	2286.75	24.25	2441.07	0.39	0.62
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	12	187.5	7.7	0.72	70.2	20.2	8.88	33.5	60.6	10.0	38.5	410765.3	20	189.0625	3.1250	25.0	0.10	7.71	0.02	2390	36	2315	40	1075	71	2.10	0.12	1.33	0.07	26	7.57	52.14	1524.21	1529.40	2251.82	58.66	2362.62	0.95	1.50
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	12	187.5	7.7	0.68	70.1	21.3	7.92	29.2	61.2	3.2	34.2	418835.4	20	189.0625	3.1250	25.0	0.10	7.71	0.02	2390	36	2315	40	1075	71	2.10	0.12	1.33	0.07	26	7.57	52.14	1524.21	1529.40	2251.82	58.66	2362.62	0.95	1.50
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	12	187.5	7.7	0.70	70.3	20.6	8.40	33.7	58.1	8.0	38.6	402383.4	20	189.0625	3.1250	25.0	0.10	7.71	0.02	2390	36	2315	40	1075	71	2.10	0.12	1.33	0.07	26	7.57	52.14	1524.21	1529.40	2251.82	58.66	2362.62	0.95	1.50
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	12	187.5	8.1	0.72	70.2	21.2	7.88	35.1	65.1	10.0	40.1	411976.5	20	190.6250	2.5000	24.9	0.20	8.11	0.01	2370	37	2137	34	378	17	4.66	0.20	2.93	0.13	26	7.97	18.95	553.77	555.65	2054.11	134.08	2207.15	2.17	3.44
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	12	187.5	8.1	0.63	70.1	21.3	7.97	30.0	63.2	2.0	35.0	420398.9	20	190.6250	2.5000	24.9	0.20	8.11	0.01	2370	37	2137	34	378	17	4.66	0.20	2.93	0.13	26	7.97	18.95	553.77	555.65	2054.11	134.08	2207.15	2.17	3.44
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	12	187.5	8.1	0.70	70.3	20.6	8.40	34.0	61.5	8.0	39.0	429957.4	20	190.6250	2.5000	24.9	0.20	8.11	0.01	2370	37	2137	34	378	17	4.66	0.20	2.93	0.13	26	7.97	18.95	553.77	555.65	2054.11	134.08	2207.15	2.17	3.44
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	12	62.5	7.3	1.01	70.0	18.1	10.89	20.6	45.2	20.6	25.7	358323.4	20	62.5000	0.3125	25.1	0.29	7.28	0.02	2131	33	2183	33	2707	139	0.72	0.04	0.45	0.03	26	7.14	129.68	3792.75	3805.71	2082.57	20.20	2232.45	0.33	0.52
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	12	62.5	7.3	1.05	68.1	18.3	12.55	17.6	43.1	22.8	22.6	341874.3	20	62.5000	0.3125	25.1	0.29	7.28	0.02	2131	33	2183	33	2707	139	0.72	0.04	0.45	0.03	26	7.14	129.68	3792.75	3805.71	2082.57	20.20	2232.45	0.33	0.52
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	12	62.5	7.3	1.24	70.3	18.6	9.86	24.7	45.9	24.2	29.7	350764.5	20	62.5000	0.3125	25.1	0.29	7.28	0.02	2131	33	2183	33	2707	139	0.72	0.04	0.45	0.03	26	7.14	129.68	3792.75	3805.71	2082.57	20.20	2232.45	0.33	0.52
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	12	62.5	7.7	0.91	70.2	18.2	10.69	20.2	59.6	20.5	25.2	362675.4	20	62.5000	0.9375	25.2	0.37	7.72	0.01	2280	28	2202	31	983	45	2.07	0.07	1.30	0.05	26	7.58	48.35	1414.77	1419.59	2143.45	57.49	2249.29	0.93	1.47
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	12	62.5	7.7	0.90	70.1	19.4	9.60	27.3	60.2	21.7	32.3	378378.9	20	62.5000	0.9375	25.2	0.37	7.72	0.01	2280	28	2202	31	983	45	2.07	0.07	1.30	0.05	26	7.58	48.35	1414.77	1419.59	2143.45	57.49	2249.29	0.93	1.47
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	12	62.5	7.7	1.10	69.2	18.6	11.10	18.6	58.1	24.0	23.5	370968.3	20	62.5000	0.9375	25.2	0.37	7.72	0.01	2280	28	2202	31	983	45	2.07	0.07	1.30	0.05	26	7.58	48.35	1414.77	1419.59	2143.45	57.49	2249.29	0.93	1.47
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	12	62.5	8.1	0.90	70.2	19.2	9.70	20.2	60.6	20.1	25.2	387674.8	20	62.5000	0.3125	25.0	0.18	8.12	0.01	2254	29	2021	33	344	19	4.56	0.21	2.87	0.13	26	7.98	17.51	511.77	513.51	1944.51	130.28	2092.29	2.10	3.34
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	12	62.5	8.1	0.89	70.1	19.3	9.71	27.4	61.1	21.2	32.4	380873.8	20	62.5000	0.3125	25.0	0.18	8.12	0.01	2254	29	2021	33	344	19	4.56	0.21	2.87	0.13	26	7.98	17.51	511.77	513.51	1944.51	130.28	2092.29	2.10	3.34
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	12	62.5	8.1	1.05	69.3	18.6	11.05	28.2	58.1	23.6	33.2	373694.6	20	62.5000	0.3125	25.0	0.18	8.12	0.01	2254	29	2021	33	344	19	4.56	0.21	2.87	0.13	26	7.98	17.51	511.77	513.51	1944.51	130.28	2092.29	2.10	3.34
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	24	187.5	7.3	0.90	68.0	17.1	14.00	31.5	45.2	11.4	36.5	400653.7	20	185.6250	2.8125	25.0	0.20	7.32	0.01	2344	39	2390	37	2710	50	0.87	0.02	0.55	0.01	26	7.18	130.06	3802.07	3815.06	2286.75	24.25	2441.07	0.39	0.62
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	24	187.5	7.3	0.92	67.1	17.3	14.68	25.2	45.5	6.5	30.2	407054.3	20	185.6250	2.8125	25.0	0.20	7.32	0.01	2344	39	2390	37	2710	50	0.87	0.02	0.55	0.01	26	7.18	130.06	3802.07	3815.06	2286.75	24.25	2441.07	0.39	0.62
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	24	187.5	7.3	0.96	68.3	17.6	13.14	26.3	47.2	10.0	31.3	414398.3	20	185.6250	2.8125	25.0	0.20	7.32	0.01	2344	39	2390	37	2710	50	0.87	0.02	0.55	0.01	26	7.18	130.06	3802.07	3815.06	2286.75	24.25	2441.07	0.39	0.62
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	24	187.5	7.7	0.84	68.2	17.0	13.96	33.5	60.6	10.1	38.4	418276.8	20	189.0625	3.1250	25.0	0.10	7.71	0.02	2390	36	2315	40	1075	71	2.10	0.12	1.33	0.07	26	7.57	52.14	1524.21	1529.40	2251.82	58.66	2362.62	0.95	1.50
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	24	187.5	7.7	0.72	67.1	18.3	13.88	25.2	62.2	2.3	30.2	410789.4	20	189.0625	3.1250	25.0	0.10	7.71	0.02	2390	36	2315	40	1075	71	2.10	0.12	1.33	0.07	26	7.57	52.14	1524.21	1529.40	2251.82	58.66	2362.62	0.95	1.50
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	24	187.5	7.7	0.80	68.3	17.6	13.30	26.6	57.9	9.0	31.6	402378.9	20	189.0625	3.1250	25.0	0.10	7.71	0.02	2390	36	2315	40	1075	71	2.10	0.12	1.33	0.07	26	7.57	52.14	1524.21	1529.40	2251.82	58.66	2362.62	0.95	1.50
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	24	187.5	8.1	0.70	70.2	21.2	7.90	35.1	65.1	10.0	40.1	422763.4	20	190.6250	2.5000	24.9	0.20	8.11	0.01	2370	37	2137	34	378	17	4.66	0.20	2.93	0.13	26	7.97	18.95	553.77	555.65	2054.11	134.08	2207.15	2.17	3.44
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	24	187.5	8.1	0.64	70.2	21.2	7.96	30.2	63.2	2.2	35.2	415836.5	20	190.6250	2.5000	24.9	0.20	8.11	0.01	2370	37	2137	34	378	17	4.66	0.20	2.93	0.13	26	7.97	18.95	553.77	555.65	2054.11	134.08	2207.15	2.17	3.44
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	24	187.5	8.1	0.78	70.3	20.6	8.32	32.9	61.5	8.1	37.8	429236.5	20	190.6250	2.5000	24.9	0.20	8.11	0.01	2370	37	2137	34	378	17	4.66	0.20	2.93	0.13	26	7.97	18.95	553.77	555.65	2054.11	134.08	2207.15	2.17	3.44
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	24	62.5	7.3	1.09	66.0	15.2	17.71	20.2	44.2	20.6	25.1	326176.4	20	62.5000	0.3125	25.1	0.29	7.28	0.02	2131	33	2183	33	2707	139	0.72	0.04	0.45	0.03	26	7.14	129.68	3792.75	3805.71	2082.57	20.20	2232.45	0.33	0.52
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	24	62.5	7.3	1.35	66.1	15.3	17.25	17.6	44.1	22.8	22.6	320578.3	20	62.5000	0.3125	25.1	0.29	7.28	0.02	2131	33	2183	33	2707	139	0.72	0.04	0.45	0.03	26	7.14	129.68	3792.75	3805.71	2082.57	20.20	2232.45	0.33	0.52
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	24	62.5	7.3	1.42	68.3	15.6	14.68	19.7	44.0	24.3	24.7	313776.5	20	62.5000	0.3125	25.1	0.29	7.28	0.02	2131	33	2183	33	2707	139	0.72	0.04	0.45	0.03	26	7.14	129.68	3792.75	3805.71	2082.57	20.20	2232.45	0.33	0.52
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	24	62.5	7.7	1.21	68.2	15.3	15.29	20.8	59.6	20.5	25.8	340765.4	20	62.5000	0.9375	25.2	0.37	7.72	0.01	2280	28	2202	31	983	45	2.07	0.07	1.30	0.05	26	7.58	48.35	1414.77	1419.59	2143.45	57.49	2249.29	0.93	1.47
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	24	62.5	7.7	1.01	67.1	16.4	15.49	17.2	60.1	21.8	22.2	347276.5	20	62.5000	0.9375	25.2	0.37	7.72	0.01	2280	28	2202	31	983	45	2.07	0.07	1.30	0.05	26	7.58	48.35	1414.77	1419.59	2143.45	57.49	2249.29	0.93	1.47
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	24	62.5	7.7	1.18	67.2	15.6	16.02	22.0	55.0	24.3	27.1	333237.6	20	62.5000	0.9375	25.2	0.37	7.72	0.01	2280	28	2202	31	983	45	2.07	0.07	1.30	0.05	26	7.58	48.35	1414.77	1419.59	2143.45	57.49	2249.29	0.93	1.47
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	24	62.5	8.1	1.21	68.2	16.1	14.49	20.6	60.2	20.1	25.6	350876.5	20	62.5000	0.3125	25.0	0.18	8.12	0.01	2254	29	2021	33	344	19	4.56	0.21	2.87	0.13	26	7.98	17.51	511.77	513.51	1944.51	130.28	2092.29	2.10	3.34
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	24	62.5	8.1	1.02	68.1	16.3	14.58	17.9	59.2	21.0	22.9	368642.8	20	62.5000	0.3125	25.0	0.18	8.12	0.01	2254	29	2021	33	344	19	4.56	0.21	2.87	0.13	26	7.98	17.51	511.77	513.51	1944.51	130.28	2092.29	2.10	3.34
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	24	62.5	8.1	0.90	67.3	15.6	16.20	23.3	58.0	23.4	28.3	360948.9	20	62.5000	0.3125	25.0	0.18	8.12	0.01	2254	29	2021	33	344	19	4.56	0.21	2.87	0.13	26	7.98	17.51	511.77	513.51	1944.51	130.28	2092.29	2.10	3.34
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	48	187.5	7.3	0.98	65.0	15.0	19.02	25.0	45.0	20.4	30.1	365365.4	20	185.6250	2.8125	25.0	0.20	7.32	0.01	2344	39	2390	37	2710	50	0.87	0.02	0.55	0.01	26	7.18	130.06	3802.07	3815.06	2286.75	24.25	2441.07	0.39	0.62
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	48	187.5	7.3	0.90	65.1	15.3	18.70	25.2	46.2	18.2	30.2	370657.8	20	185.6250	2.8125	25.0	0.20	7.32	0.01	2344	39	2390	37	2710	50	0.87	0.02	0.55	0.01	26	7.18	130.06	3802.07	3815.06	2286.75	24.25	2441.07	0.39	0.62
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	48	187.5	7.3	1.00	66.3	15.6	17.10	23.7	47.1	16.2	28.7	376197.3	20	185.6250	2.8125	25.0	0.20	7.32	0.01	2344	39	2390	37	2710	50	0.87	0.02	0.55	0.01	26	7.18	130.06	3802.07	3815.06	2286.75	24.25	2441.07	0.39	0.62
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	48	187.5	7.7	0.88	66.2	15.0	17.92	33.5	60.7	10.1	38.5	385746.9	20	189.0625	3.1250	25.0	0.10	7.71	0.02	2390	36	2315	40	1075	71	2.10	0.12	1.33	0.07	26	7.57	52.14	1524.21	1529.40	2251.82	58.66	2362.62	0.95	1.50
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	48	187.5	7.7	0.74	64.1	16.1	19.06	25.2	55.0	3.4	30.2	376359.6	20	189.0625	3.1250	25.0	0.10	7.71	0.02	2390	36	2315	40	1075	71	2.10	0.12	1.33	0.07	26	7.57	52.14	1524.21	1529.40	2251.82	58.66	2362.62	0.95	1.50
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	48	187.5	7.7	0.85	66.3	15.6	17.25	26.4	56.8	9.2	31.4	393397.3	20	189.0625	3.1250	25.0	0.10	7.71	0.02	2390	36	2315	40	1075	71	2.10	0.12	1.33	0.07	26	7.57	52.14	1524.21	1529.40	2251.82	58.66	2362.62	0.95	1.50
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	48	187.5	8.1	0.72	70.1	21.0	8.18	35.1	65.1	10.0	40.2	428365.8	20	190.6250	2.5000	24.9	0.20	8.11	0.01	2370	37	2137	34	378	17	4.66	0.20	2.93	0.13	26	7.97	18.95	553.77	555.65	2054.11	134.08	2207.15	2.17	3.44
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	48	187.5	8.1	0.65	70.1	21.3	7.95	30.7	63.3	2.3	35.7	414457.3	20	190.6250	2.5000	24.9	0.20	8.11	0.01	2370	37	2137	34	378	17	4.66	0.20	2.93	0.13	26	7.97	18.95	553.77	555.65	2054.11	134.08	2207.15	2.17	3.44
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	48	187.5	8.1	0.80	70.3	20.6	8.30	33.4	61.4	8.2	38.4	421979.4	20	190.6250	2.5000	24.9	0.20	8.11	0.01	2370	37	2137	34	378	17	4.66	0.20	2.93	0.13	26	7.97	18.95	553.77	555.65	2054.11	134.08	2207.15	2.17	3.44
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	48	62.5	7.3	2.00	62.0	13.3	22.70	15.1	44.2	22.5	20.1	317569.3	20	62.5000	0.3125	25.1	0.29	7.28	0.02	2131	33	2183	33	2707	139	0.72	0.04	0.45	0.03	26	7.14	129.68	3792.75	3805.71	2082.57	20.20	2232.45	0.33	0.52
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	48	62.5	7.3	1.98	64.1	13.3	20.62	17.6	44.2	23.4	22.6	304538.5	20	62.5000	0.3125	25.1	0.29	7.28	0.02	2131	33	2183	33	2707	139	0.72	0.04	0.45	0.03	26	7.14	129.68	3792.75	3805.71	2082.57	20.20	2232.45	0.33	0.52
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	48	62.5	7.3	2.02	66.3	13.6	18.08	18.6	42.9	24.5	23.5	310853.8	20	62.5000	0.3125	25.1	0.29	7.28	0.02	2131	33	2183	33	2707	139	0.72	0.04	0.45	0.03	26	7.14	129.68	3792.75	3805.71	2082.57	20.20	2232.45	0.33	0.52
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	48	62.5	7.7	2.00	64.2	13.5	20.30	15.3	44.2	22.4	20.3	310593.7	20	62.5000	0.9375	25.2	0.37	7.72	0.01	2280	28	2202	31	983	45	2.07	0.07	1.30	0.05	26	7.58	48.35	1414.77	1419.59	2143.45	57.49	2249.29	0.93	1.47
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	48	62.5	7.7	1.80	65.1	14.4	18.70	18.2	44.1	23.6	23.2	316639.3	20	62.5000	0.9375	25.2	0.37	7.72	0.01	2280	28	2202	31	983	45	2.07	0.07	1.30	0.05	26	7.58	48.35	1414.77	1419.59	2143.45	57.49	2249.29	0.93	1.47
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	48	62.5	7.7	1.98	65.2	13.6	19.22	18.6	43.7	23.2	23.5	305139.4	20	62.5000	0.9375	25.2	0.37	7.72	0.01	2280	28	2202	31	983	45	2.07	0.07	1.30	0.05	26	7.58	48.35	1414.77	1419.59	2143.45	57.49	2249.29	0.93	1.47
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	48	62.5	8.1	1.80	66.2	14.5	17.50	15.3	44.4	25.4	20.3	312784.3	20	62.5000	0.3125	25.0	0.18	8.12	0.01	2254	29	2021	33	344	19	4.56	0.21	2.87	0.13	26	7.98	17.51	511.77	513.51	1944.51	130.28	2092.29	2.10	3.34
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	48	62.5	8.1	1.95	66.1	14.3	17.65	18.3	44.2	23.7	23.3	328276.4	20	62.5000	0.3125	25.0	0.18	8.12	0.01	2254	29	2021	33	344	19	4.56	0.21	2.87	0.13	26	7.98	17.51	511.77	513.51	1944.51	130.28	2092.29	2.10	3.34
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	48	62.5	8.1	1.92	65.3	13.6	19.18	19.8	44.0	22.9	24.8	320938.7	20	62.5000	0.3125	25.0	0.18	8.12	0.01	2254	29	2021	33	344	19	4.56	0.21	2.87	0.13	26	7.98	17.51	511.77	513.51	1944.51	130.28	2092.29	2.10	3.34
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	72	187.5	7.3	0.88	59.0	13.6	26.52	20.0	45.0	20.4	25.0	361537.3	20	185.6250	2.8125	25.0	0.20	7.32	0.01	2344	39	2390	37	2710	50	0.87	0.02	0.55	0.01	26	7.18	130.06	3802.07	3815.06	2286.75	24.25	2441.07	0.39	0.62
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	72	187.5	7.3	1.14	60.3	13.8	24.76	22.8	46.2	19.2	27.8	379669.7	20	185.6250	2.8125	25.0	0.20	7.32	0.01	2344	39	2390	37	2710	50	0.87	0.02	0.55	0.01	26	7.18	130.06	3802.07	3815.06	2286.75	24.25	2441.07	0.39	0.62
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	72	187.5	7.3	0.94	61.3	14.1	23.66	21.2	47.1	17.3	26.2	370443.7	20	185.6250	2.8125	25.0	0.20	7.32	0.01	2344	39	2390	37	2710	50	0.87	0.02	0.55	0.01	26	7.18	130.06	3802.07	3815.06	2286.75	24.25	2441.07	0.39	0.62
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	72	187.5	7.7	0.88	60.2	13.5	25.42	33.5	60.7	10.1	38.5	372656.7	20	189.0625	3.1250	25.0	0.10	7.71	0.02	2390	36	2315	40	1075	71	2.10	0.12	1.33	0.07	26	7.57	52.14	1524.21	1529.40	2251.82	58.66	2362.62	0.95	1.50
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	72	187.5	7.7	0.90	59.2	14.7	25.20	25.4	61.7	3.6	30.4	380954.7	20	189.0625	3.1250	25.0	0.10	7.71	0.02	2390	36	2315	40	1075	71	2.10	0.12	1.33	0.07	26	7.57	52.14	1524.21	1529.40	2251.82	58.66	2362.62	0.95	1.50
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	72	187.5	7.7	0.83	61.3	14.1	23.77	25.7	56.0	9.2	30.6	389658.4	20	189.0625	3.1250	25.0	0.10	7.71	0.02	2390	36	2315	40	1075	71	2.10	0.12	1.33	0.07	26	7.57	52.14	1524.21	1529.40	2251.82	58.66	2362.62	0.95	1.50
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	72	187.5	8.1	0.74	61.2	21.3	16.76	35.1	65.1	10.1	40.1	411875.4	20	190.6250	2.5000	24.9	0.20	8.11	0.01	2370	37	2137	34	378	17	4.66	0.20	2.93	0.13	26	7.97	18.95	553.77	555.65	2054.11	134.08	2207.15	2.17	3.44
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	72	187.5	8.1	0.65	61.1	21.3	16.95	30.6	63.3	2.3	35.6	420574.3	20	190.6250	2.5000	24.9	0.20	8.11	0.01	2370	37	2137	34	378	17	4.66	0.20	2.93	0.13	26	7.97	18.95	553.77	555.65	2054.11	134.08	2207.15	2.17	3.44
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	72	187.5	8.1	0.78	61.3	20.6	17.32	33.8	61.5	6.2	38.8	429642.8	20	190.6250	2.5000	24.9	0.20	8.11	0.01	2370	37	2137	34	378	17	4.66	0.20	2.93	0.13	26	7.97	18.95	553.77	555.65	2054.11	134.08	2207.15	2.17	3.44
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	72	62.5	7.3	3.00	55.0	11.9	30.10	10.1	44.2	22.5	15.1	310864.7	20	62.5000	0.3125	25.1	0.29	7.28	0.02	2131	33	2183	33	2707	139	0.72	0.04	0.45	0.03	26	7.14	129.68	3792.75	3805.71	2082.57	20.20	2232.45	0.33	0.52
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	72	62.5	7.3	3.20	59.1	11.8	25.90	9.7	44.0	23.8	14.6	317573.4	20	62.5000	0.3125	25.1	0.29	7.28	0.02	2131	33	2183	33	2707	139	0.72	0.04	0.45	0.03	26	7.14	129.68	3792.75	3805.71	2082.57	20.20	2232.45	0.33	0.52
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	72	62.5	7.3	3.45	61.3	12.1	23.15	8.2	42.6	25.0	13.2	304297.6	20	62.5000	0.3125	25.1	0.29	7.28	0.02	2131	33	2183	33	2707	139	0.72	0.04	0.45	0.03	26	7.14	129.68	3792.75	3805.71	2082.57	20.20	2232.45	0.33	0.52
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	72	62.5	7.7	2.52	56.2	12.1	29.18	10.2	44.2	22.6	15.2	316023.9	20	62.5000	0.9375	25.2	0.37	7.72	0.01	2280	28	2202	31	983	45	2.07	0.07	1.30	0.05	26	7.58	48.35	1414.77	1419.59	2143.45	57.49	2249.29	0.93	1.47
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	72	62.5	7.7	2.48	60.1	12.9	24.52	8.8	44.0	23.9	13.7	304073.6	20	62.5000	0.9375	25.2	0.37	7.72	0.01	2280	28	2202	31	983	45	2.07	0.07	1.30	0.05	26	7.58	48.35	1414.77	1419.59	2143.45	57.49	2249.29	0.93	1.47
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	72	62.5	7.7	2.35	60.2	12.1	25.35	8.9	43.6	26.1	13.9	310568.3	20	62.5000	0.9375	25.2	0.37	7.72	0.01	2280	28	2202	31	983	45	2.07	0.07	1.30	0.05	26	7.58	48.35	1414.77	1419.59	2143.45	57.49	2249.29	0.93	1.47
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	72	62.5	8.1	2.50	70.2	13.2	14.10	10.3	44.4	21.7	15.3	320783.6	20	62.5000	0.3125	25.0	0.18	8.12	0.01	2254	29	2021	33	344	19	4.56	0.21	2.87	0.13	26	7.98	17.51	511.77	513.51	1944.51	130.28	2092.29	2.10	3.34
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	72	62.5	8.1	2.42	70.1	12.8	14.68	9.9	44.6	23.9	13.8	327463.9	20	62.5000	0.3125	25.0	0.18	8.12	0.01	2254	29	2021	33	344	19	4.56	0.21	2.87	0.13	26	7.98	17.51	511.77	513.51	1944.51	130.28	2092.29	2.10	3.34
laboratory	Mytilus coruscus (mollusk)	506159	marinespecies.org	72	62.5	8.1	2.31	70.3	12.1	15.29	9.7	43.9	26.0	14.7	313983.4	20	62.5000	0.3125	25.0	0.18	8.12	0.01	2254	29	2021	33	344	19	4.56	0.21	2.87	0.13	26	7.98	17.51	511.77	513.51	1944.51	130.28	2092.29	2.10	3.34