Nardi, Alessandro; Mincarelli, Luana Fiorella; Benedetti, Maura; Fattorini, Daniele; d'Errico, Giuseppe; Regoli, Francesco (2017): Indirect effects of climate changes on cadmium bioavailability and biological effects in the Mediterranean mussel Mytilus galloprovincialis [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.872084, Supplement to: Nardi, A et al. (2017): Indirect effects of climate changes on cadmium bioavailability and biological effects in the Mediterranean mussel Mytilus galloprovincialis. Chemosphere, 169, 493-502, https://doi.org/10.1016/j.chemosphere.2016.11.093
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Abstract:
Despite the great interest in the consequences of climate change on the physiological functioning of marine organisms, indirect and interactive effects of rising temperature and pCO2 on bioaccumulation and responsiveness to environmental pollutants are still poorly explored, particularly in terms of cellular mechanisms. According to future projections of temperature and pH/pCO2, this study investigated the main cellular pathways involved in metal detoxification and oxidative homeostasis in Mediterranean mussels, Mytilus galloprovincialis, exposed for 4 weeks to various combinations of two levels of pH/pCO2 (8.2/400 µatm and 7.4/3000 µatm), temperature (20 and 25 °C), and cadmium addition (0 and 20 µg/L). Bioaccumulation was increased in metal exposed organisms but it was not further modulated by different temperature and pH/pCO2 combinations. However, interactions between temperature, pH and cadmium had significant effects on induction of metallothioneins, responses of the antioxidant system and the onset of oxidative damages, which was tissue dependent. Multiple stressors increased metallothioneins concentrations in the digestive gland revealing different oxidative effects: while temperature and cadmium enhanced glutathione-dependent antioxidant protection and capability to neutralize peroxyl radicals, the metal increased the accumulation of lipid peroxidation products under acidified conditions. Gills did not reveal specific effects for different combinations of factors, but a general stress condition was observed in this tissue after various treatments. Significant variations of immune system were mainly caused by increased temperature and low pH, while co-exposure to acidification and cadmium enhanced metal genotoxicity and the onset of permanent DNA damage in haemocytes. Elaboration of the whole biomarker data in a cellular hazard index, corroborated the synergistic effects of temperature and acidification which increased the toxicological effects of cadmium. The overall results confirmed that climate change could influence ecotoxicological effects of environmental contaminants, highlighting the importance of a better knowledge of cellular mechanisms to understand and predict responsiveness of marine organisms to such multiple stressors.
Keyword(s):
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb
Project(s):
Coverage:
Latitude: 43.500000 * Longitude: 13.700000
Date/Time Start: 2014-06-01T00:00:00 * Date/Time End: 2014-06-30T00:00:00
Event(s):
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2017-02-14.
Parameter(s):
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
712 data points
Data
1 Type (study) | 2 Species | 3 Reg spec no | 4 URL ref (WoRMS Aphia ID) | 5 Treat | 6 Sample label | 7 Inc dur [days] | 8 Sal | 9 Sal std dev [±] | 10 Temp [°C] | 11 Temp std dev [±] | 12 pH (NBS scale, Potentiometric) | 13 pH std dev [±] (NBS scale, Potentiometric) | 14 AT [µmol/kg] (Potentiometric titration) | 15 AT std dev [±] (Potentiometric titration) | 16 pCO2water_SST_wet [µatm] (Calculated using CO2SYS) | 17 pCO2 std dev [±] (Calculated using CO2SYS) | 18 Omega Cal (Calculated using CO2SYS) | 19 Omega Cal std dev [±] (Calculated using CO2SYS) | 20 Omega Arg (Calculated using CO2SYS) | 21 Omega Arg std dev [±] (Calculated using CO2SYS) | 22 Cd [mg/kg] (in digestive gland) | 23 Cd std dev [±] (in digestive gland) | 24 MT/prot [nmol/mg] (in digestive gland) | 25 MT std dev [±] (in digestive gland) | 26 TGSH [µmol/g] (in digestive gland) | 27 TGSH std dev [±] (in digestive gland) | 28 CAT/prot [µmol/min/mg] (in digestive gland) | 29 CAT std dev [±] (in digestive gland) | 30 GST/prot [nmol/min/mg] (in digestive gland) | 31 GST std dev [±] (in digestive gland) | 32 GR/prot [nmol/min/mg] (in digestive gland) | 33 GR std dev [±] (in digestive gland) | 34 GPxSe-Dep/prot [nmol/min/mg] (in digestive gland) | 35 GPxSe-Dep std dev [±] (in digestive gland) | 36 GPxTOT/prot [nmol/min/mg] (in digestive gland) | 37 GPxTOT std dev [±] (in digestive gland) | 38 TOSC-ROO [U/mg] (in digestive gland) | 39 TOSC-ROO std dev [±] (in digestive gland) | 40 TOSC-HO [U/mg] (in digestive gland) | 41 TOSC-HO std dev [±] (in digestive gland) | 42 MDA [nmol/g] (in digestive gland) | 43 MDA std dev [±] (in digestive gland) | 44 Lipo [intensity/µm2] (in digestive gland) | 45 Lipo std dev [±] (in digestive gland) | 46 NL [intensity/µm2] (in digestive gland) | 47 NL std dev [±] (in digestive gland) | 48 Cd [mg/kg] (in gills) | 49 Cd std dev [±] (in gills) | 50 MT/prot [nmol/mg] (in gills) | 51 MT std dev [±] (in gills) | 52 TGSH [µmol/g] (in gills) | 53 TGSH std dev [±] (in gills) | 54 CAT/prot [µmol/min/mg] (in gills) | 55 CAT std dev [±] (in gills) | 56 GST/prot [nmol/min/mg] (in gills) | 57 GST std dev [±] (in gills) | 58 GR/prot [nmol/min/mg] (in gills) | 59 GR std dev [±] (in gills) | 60 GPxSe-Dep/prot [nmol/min/mg] (in gills) | 61 GPxSe-Dep std dev [±] (in gills) | 62 GPxTOT/prot [nmol/min/mg] (in gills) | 63 GPxTOT std dev [±] (in gills) | 64 TOSC-ROO [U/mg] (in gills) | 65 TOSC-ROO std dev [±] (in gills) | 66 TOSC-HO [U/mg] (in gills) | 67 TOSC-HO std dev [±] (in gills) | 68 MDA [nmol/g] (in gills) | 69 MDA std dev [±] (in gills) | 70 LMS [min] | 71 LMS std dev [±] | 72 Phagocytosis rate [%] (haemocytes) | 73 Phago std dev [±] | 74 G/H | 75 G/H std dev [±] | 76 DNA damage [%] | 77 DNA damage std dev [±] | 78 Hem MN [‰] | 79 Hem MN std dev [±] | 80 CSC flag (Calculated using seacarb afte...) | 81 pH (total scale, Calculated using...) | 82 CO2 [µmol/kg] (Calculated using seacarb afte...) | 83 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...) | 84 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...) | 85 [HCO3]- [µmol/kg] (Calculated using seacarb afte...) | 86 [CO3]2- [µmol/kg] (Calculated using seacarb afte...) | 87 DIC [µmol/kg] (Calculated using seacarb afte...) | 88 Omega Arg (Calculated using seacarb afte...) | 89 Omega Cal (Calculated using seacarb afte...) |
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laboratory | Mytilus galloprovincialis (mollusk) | 140481 | marinespecies.org | CONTROL | CTRL | 28 | 37 | 0.5 | 19.95 | 0.10 | 8.21 | 0.04 | 2453.6 | 251.5 | 380.8 | 25.8 | 5.3 | 0.4 | 3.5 | 0.2 | 2.89 | 1.91 | 3.66 | 1.38 | 0.67 | 0.12 | 27.94 | 5.20 | 64.74 | 7.28 | 10.05 | 4.48 | 0.98 | 0.47 | 6.17 | 0.45 | 306.51 | 39.10 | 304.49 | 34.63 | 59.28 | 27.26 | 35.39 | 6.45 | 30.65 | 15.19 | 1.04 | 0.62 | 2.04 | 0.28 | 0.24 | 0.03 | 26.69 | 3.53 | 895.53 | 169.97 | 13.63 | 6.22 | 1.86 | 0.31 | 20.50 | 2.61 | 202.92 | 31.32 | 903.80 | 74.71 | 25.41 | 7.32 | 81.97 | 9.23 | 74.91 | 6.44 | 5.56 | 1.75 | 11.38 | 5.66 | 0.00 | 0.00 | 26 | 8.09 | 11.99 | 373.38 | 374.66 | 1913.55 | 219.98 | 2145.52 | 3.38 | 5.18 |
laboratory | Mytilus galloprovincialis (mollusk) | 140481 | marinespecies.org | CONTROL + Cd | Cd | 28 | 37 | 0.5 | 20.00 | 0.10 | 8.19 | 0.04 | 2390.5 | 354.1 | 410.6 | 30.9 | 5.1 | 0.4 | 3.3 | 0.3 | 33.15 | 3.70 | 6.68 | 0.95 | 0.89 | 0.07 | 19.47 | 4.65 | 77.69 | 23.13 | 9.56 | 2.28 | 1.81 | 0.72 | 4.83 | 1.23 | 266.00 | 30.03 | 269.88 | 30.28 | 69.59 | 16.52 | 70.23 | 8.45 | 57.81 | 16.76 | 14.22 | 1.04 | 7.81 | 1.76 | 0.24 | 0.02 | 20.71 | 0.98 | 759.64 | 117.42 | 24.49 | 4.87 | 3.92 | 1.76 | 18.80 | 3.37 | 263.26 | 33.04 | 688.57 | 148.95 | 29.59 | 14.87 | 69.36 | 10.49 | 71.71 | 9.15 | 10.26 | 1.94 | 12.16 | 2.60 | 0.63 | 0.48 | 26 | 8.07 | 12.33 | 384.45 | 385.76 | 1880.40 | 206.76 | 2099.49 | 3.17 | 4.87 |
laboratory | Mytilus galloprovincialis (mollusk) | 140481 | marinespecies.org | REDUCED pH | A | 28 | 37 | 0.5 | 19.98 | 0.06 | 7.42 | 0.04 | 2557.3 | 183.7 | 2897.6 | 183.8 | 1.0 | 0.1 | 0.7 | 0.1 | 3.01 | 1.08 | 5.27 | 1.17 | 0.81 | 0.09 | 22.95 | 6.39 | 83.36 | 3.88 | 10.95 | 1.80 | 1.99 | 0.77 | 7.37 | 1.18 | 307.02 | 12.15 | 334.58 | 46.84 | 79.64 | 23.33 | 73.63 | 4.94 | 17.31 | 5.21 | 0.55 | 0.26 | 2.32 | 0.55 | 0.30 | 0.03 | 24.32 | 3.12 | 714.55 | 142.67 | 25.32 | 8.67 | 5.98 | 2.58 | 5.64 | 2.55 | 146.90 | 18.24 | 539.21 | 19.65 | 24.65 | 5.25 | 36.29 | 4.24 | 73.16 | 11.92 | 5.87 | 0.92 | 14.75 | 5.80 | 0.25 | 0.29 | 26 | 7.30 | 94.54 | 2947.26 | 2957.32 | 2446.49 | 45.61 | 2586.64 | 0.70 | 1.07 |
laboratory | Mytilus galloprovincialis (mollusk) | 140481 | marinespecies.org | HIGHER TEMPERATURE | W | 28 | 37 | 0.5 | 24.80 | 0.13 | 8.15 | 0.06 | 2325.4 | 267.7 | 468.1 | 47.9 | 5.4 | 0.4 | 3.6 | 0.3 | 2.83 | 1.15 | 4.07 | 0.86 | 0.86 | 0.07 | 22.42 | 7.08 | 95.26 | 14.00 | 12.08 | 3.27 | 2.93 | 1.28 | 12.60 | 1.68 | 434.33 | 92.61 | 327.91 | 44.96 | 53.87 | 24.40 | 47.26 | 9.92 | 35.59 | 16.20 | 0.38 | 0.12 | 4.66 | 1.89 | 0.19 | 0.03 | 21.69 | 2.16 | 702.56 | 67.98 | 24.86 | 6.20 | 3.55 | 1.72 | 16.04 | 2.47 | 174.99 | 30.73 | 546.62 | 134.10 | 26.60 | 9.53 | 40.55 | 8.00 | 56.07 | 7.94 | 5.36 | 1.16 | 9.05 | 2.65 | 0.00 | 0.00 | 26 | 8.02 | 12.09 | 428.29 | 429.66 | 1805.38 | 210.47 | 2027.94 | 3.29 | 4.98 |
laboratory | Mytilus galloprovincialis (mollusk) | 140481 | marinespecies.org | REDUCED pH + Cd | A-Cd | 28 | 37 | 0.5 | 19.95 | 0.06 | 7.41 | 0.04 | 2556.7 | 479.0 | 2928.2 | 144.4 | 1.0 | 0.1 | 0.7 | 0.1 | 34.53 | 9.48 | 8.65 | 0.53 | 0.86 | 0.11 | 17.81 | 2.08 | 92.92 | 20.82 | 10.96 | 5.57 | 2.58 | 0.38 | 5.41 | 1.00 | 226.82 | 24.83 | 231.66 | 65.36 | 45.59 | 24.60 | 87.51 | 2.70 | 28.01 | 8.72 | 19.47 | 2.40 | 4.39 | 1.27 | 0.20 | 0.03 | 21.35 | 3.44 | 890.87 | 225.31 | 19.81 | 0.93 | 1.89 | 1.55 | 20.57 | 4.60 | 228.67 | 61.19 | 683.54 | 131.61 | 33.09 | 12.08 | 28.17 | 6.45 | 75.84 | 7.66 | 7.74 | 0.55 | 13.82 | 2.01 | 1.88 | 1.30 | 26 | 7.29 | 96.86 | 3017.31 | 3027.62 | 2448.43 | 44.57 | 2589.86 | 0.68 | 1.05 |
laboratory | Mytilus galloprovincialis (mollusk) | 140481 | marinespecies.org | HIGHER TEMPERATURE + Cd | W-Cd | 28 | 37 | 0.5 | 24.83 | 0.08 | 8.14 | 0.04 | 2517.9 | 206.9 | 477.4 | 44.6 | 5.2 | 0.4 | 3.5 | 0.2 | 38.03 | 10.62 | 7.37 | 1.22 | 0.72 | 0.25 | 22.29 | 5.81 | 118.77 | 26.24 | 17.34 | 4.41 | 3.19 | 0.59 | 5.34 | 1.04 | 253.22 | 3.52 | 299.61 | 83.80 | 77.03 | 29.37 | 54.55 | 18.16 | 44.26 | 12.99 | 18.78 | 8.93 | 5.50 | 0.85 | 0.19 | 0.04 | 24.67 | 2.52 | 774.00 | 23.03 | 10.43 | 1.66 | 8.12 | 4.10 | 10.22 | 2.28 | 306.36 | 13.45 | 569.83 | 52.24 | 22.50 | 7.88 | 38.94 | 12.79 | 52.50 | 7.68 | 7.89 | 1.47 | 9.03 | 1.94 | 0.50 | 0.58 | 26 | 8.01 | 13.51 | 478.63 | 480.16 | 1971.04 | 224.76 | 2209.31 | 3.51 | 5.32 |
laboratory | Mytilus galloprovincialis (mollusk) | 140481 | marinespecies.org | REDUCED pH + HIGHER TEMPERATURE | A-W | 28 | 37 | 0.5 | 24.76 | 0.18 | 7.42 | 0.03 | 2721.4 | 215.7 | 3100.1 | 241.7 | 1.2 | 0.1 | 0.8 | 0.1 | 5.08 | 0.12 | 5.37 | 1.26 | 0.96 | 0.25 | 25.96 | 7.78 | 100.97 | 13.39 | 14.17 | 3.40 | 1.83 | 0.27 | 4.33 | 1.24 | 295.79 | 88.24 | 232.31 | 68.99 | 27.14 | 5.45 | 35.53 | 12.54 | 62.84 | 10.48 | 0.33 | 0.13 | 3.68 | 1.16 | 0.26 | 0.04 | 24.37 | 2.13 | 707.24 | 295.03 | 34.18 | 7.24 | 3.15 | 2.79 | 8.52 | 2.97 | 169.71 | 53.90 | 522.32 | 13.08 | 23.19 | 3.99 | 28.56 | 3.93 | 62.75 | 10.87 | 7.85 | 2.04 | 13.53 | 1.29 | 0.13 | 0.25 | 26 | 7.29 | 93.15 | 3295.01 | 3305.62 | 2587.30 | 56.09 | 2736.53 | 0.88 | 1.33 |
laboratory | Mytilus galloprovincialis (mollusk) | 140481 | marinespecies.org | REDUCED pH + HIGHER TEMPERATURE + Cd | A-W-Cd | 28 | 37 | 0.5 | 24.87 | 0.16 | 7.43 | 0.04 | 2504.2 | 182.0 | 2993.7 | 186.7 | 1.3 | 0.1 | 0.9 | 0.1 | 39.80 | 5.23 | 11.04 | 1.58 | 0.85 | 0.13 | 30.33 | 17.45 | 116.01 | 31.36 | 15.92 | 5.67 | 3.20 | 1.66 | 7.20 | 2.02 | 237.24 | 12.77 | 239.95 | 49.46 | 33.61 | 7.15 | 58.84 | 13.82 | 44.21 | 3.24 | 15.99 | 4.40 | 5.14 | 0.90 | 0.22 | 0.06 | 26.73 | 1.77 | 896.16 | 51.61 | 31.83 | 17.54 | 1.45 | 0.68 | 16.33 | 1.01 | 240.72 | 14.63 | 669.86 | 181.11 | 25.03 | 4.23 | 32.00 | 7.06 | 55.25 | 6.99 | 14.57 | 4.75 | 10.94 | 1.91 | 2.00 | 1.40 | 26 | 7.30 | 83.46 | 2960.71 | 2970.23 | 2375.95 | 52.89 | 2512.30 | 0.83 | 1.25 |