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Knapp, Jarred L; Bridges, Christopher R; Krohn, Janina; Hoffman, Louwrens C; Auerswald, Lutz (2017): The effects of hypercapnia on the West Coast rock lobster (Jasus lalandii) through acute exposure to decreased seawater pH-Physiological and biochemical responses. PANGAEA, https://doi.org/10.1594/PANGAEA.871938, Supplement to: Knapp, JL et al. (2016): The effects of hypercapnia on the West Coast rock lobster (Jasus lalandii) through acute exposure to decreased seawater pH-Physiological and biochemical responses. Journal of Experimental Marine Biology and Ecology, 476, 58-64, https://doi.org/10.1016/j.jembe.2015.12.001

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Abstract:
The cold water palinurid Jasus lalandii ('West Coast rock lobster') is a commercially important crustacean in South Africa and Namibia and inhabits the Benguela Current Eastern Boundary System. This habitat is characterised by strong upwelling events in summer and algal blooms with their subsequent decay in autumn. Upwelling can lead to acute hypercapnia whereas the algal decay is associated with acute hypercapnic hypoxia. Both types of hypercapnic events could become more frequent and severe in the future due to ongoing climate change. The aim of the present study was, however, to study the capability and mechanisms of response in J. lalandii to hypercapnia exclusively. Accordingly, the following research questions were formulated: 1) To what extent is haemocyanin oxygen-binding affinity of adult J. lalandii pH-sensitive? 2) Can adult male J. lalandii respond swiftly to drastic changes in pH? 3) What physiological mechanisms facilitate a potential response to a drastically declining pH, i.e. acute hypercapnia? These questions were answered by analysing 1) the pH sensitivity of the haemocyanin's oxygen binding properties and 2) in vivo changes in the acid-base balance of adult J. lalandii during acute exposure to hypercapnia (pH 7.4). Results showed the following: 1) Haemocyanin displays a strong Bohr shift (whole haemolymph: delta logP50/delta pH = -1.17; dialysed haemolymph: delta logP50/delta pH = -0.84) in response to lowering of pH. 2) Acute hypercapnia leads to a decline in extracellular pH within the initial 1.5 h of exposure. 3) Thereafter, active compensation becomes apparent as the bicarbonate levels start to increase, with complete compensation reached after 5 h of exposure (+ 2.3 mmol/l; + 48%). 3) This bicarbonate increase is reversed when returning lobsters to normocapnia (pH 7.9). 4) Levels of molecular modulators of haemocyanin oxygen affinity (Ca2 +, Mg2 + and l-lactate) do not change during acute exposure to hypercapnia.
Our results show the capability of adult J. lalandii to rapidly and fully compensate the experienced extracellular acidosis, protecting oxygen carrying capacity of haemocyanin and ensuring an outward gradient of CO2. The West Coast rock lobster is therefore well equipped for its habitat where these hypercapnic events are known to occur frequently.
Keyword(s):
Acid-base regulation; Animalia; Arthropoda; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria (20- 1000 L or < 1 m**2); Jasus lalandii; Laboratory experiment; Single species; South Atlantic; Temperate
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloise; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb
Coverage:
Latitude: -34.070000 * Longitude: 18.330000
Event(s):
Cape_Town_OA * Latitude: -34.070000 * Longitude: 18.330000 * Device: Experiment (EXP)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2017-02-10.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1TypeTypeAuerswald, Lutzstudy
2SpeciesSpeciesAuerswald, Lutz
3Registration number of speciesReg spec noAuerswald, Lutz
4Uniform resource locator/link to referenceURL refAuerswald, LutzWoRMS Aphia ID
5TreatmentTreatAuerswald, Lutz
6Time in hoursTimehAuerswald, Lutzexposure
7Haemolymph, pHpH (ha)Auerswald, LutzNBS scale
8Haemolymph, pH, standard errorpH (ha) std e±Auerswald, LutzNBS scale
9Haemolymph, total carbon dioxideTCO2 (ha)mmol/lAuerswald, Lutz
10Haemolymph, total carbon dioxide, standard errorTCO2 (ha) std e±Auerswald, Lutz
11Haemolymph, carbon dioxide tensionPCO2 (ha)TorrAuerswald, Lutz
12Haemolymph, partial pressure of carbon dioxide, standard errorpCO2 (ha) std e±Auerswald, Lutz
13Haemolymph, partial pressure of carbon dioxidepCO2 (ha)kPaAuerswald, Lutz
14Haemolymph, partial pressure of carbon dioxide, standard errorpCO2 (ha) std e±Auerswald, Lutz
15Haemolymph, bicarbonate ion + carbonate ion[HCO3]- + [CO3]2- (ha)mmol/lAuerswald, Lutz
16Haemolymph, bicarbonate ion + carbonate ion, standard error[HCO3]- + [CO3]2- (ha) std e±Auerswald, Lutz
17Haemolymph, calcium ionCa2+ (ha)mmol/lAuerswald, Lutz
18Haemolymph, calcium ion, standard errorCa2+ (ha) std e±Auerswald, Lutz
19Haemolymph, magnesium ionMg2+ (ha)mmol/lAuerswald, Lutz
20Haemolymph, magnesium ion, standard errorMg2+ (ha) std e±Auerswald, Lutz
21Haemolymph, lactateLactate (ha)mmol/lAuerswald, Lutz
22Haemolymph, lactate, standard errorLactate (ha) std e±Auerswald, Lutz
23Haemolymph, haemocyaninHaemocyanin (ha)mg/mlAuerswald, Lutz
24Haemolymph, haemocyanin, standard errorHaemocyanin (ha) std e±Auerswald, Lutz
25Temperature, waterTemp°CAuerswald, Lutz
26Temperature, water, standard errorT std e±Auerswald, Lutz
27pHpHAuerswald, LutzNBS scale
28pH, standard errorpH std e±Auerswald, LutzNBS scale
29Alkalinity, totalATµmol/kgAuerswald, Lutz
30Alkalinity, total, standard errorAT std e±Auerswald, Lutz
31Oxygen saturationO2 sat%Auerswald, Lutz
32SalinitySalAuerswald, Lutz
33Salinity, standard errorSal std e±Auerswald, Lutz
34Calcium ionCa2+mmol/lAuerswald, Lutz
35Calcium ion, standard errorCa2+ std e±Auerswald, Lutz
36Magnesium ionMg2+mmol/lAuerswald, Lutz
37Magnesium ion, standard errorMg2+ std e±Auerswald, Lutz
38Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetTorrAuerswald, Lutz
39Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard errorpCO2water_SST_wet std e±Auerswald, Lutz
40Carbon dioxide, partial pressurepCO2PaAuerswald, Lutz
41Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard errorpCO2water_SST_wet std e±Auerswald, Lutz
42Bicarbonate[HCO3]-mmol/lAuerswald, Lutz
43Bicarbonate ion, standard error[HCO3]- std e±Auerswald, Lutz
44Carbonate ion[CO3]2-µmol/lAuerswald, Lutz
45Carbonate ion, standard error[CO3]2- std e±Auerswald, Lutz
46Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
47pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
48Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
49Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
50Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
51Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
52Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
53Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
54Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
55Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
Size:
770 data points

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