Citation:

meps11791

Name: Otolith size and the vestibulo-ocular reflex of larvae of white seabass Atractoscion nobilis at high pCO2
Published: 2016-12-23
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Animalia; Atractoscion nobilis; Behaviour; Bottles or small containers/Aquaria (<20 L); Chordata; Coast and continental shelf; Growth/Morphology; Laboratory experiment; Nekton; North Pacific; Pelagos; Single species; Temperate

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Abstract:

We investigated vestibular function and otolith size (OS) in larvae of white seabass Atractoscion nobilis exposed to high partial pressure of CO2 (pCO2). The context for our study is the increasing concentration of CO2 in seawater that is causing ocean acidification (OA). The utricular otoliths are aragonitic structures in the inner ear of fish that act to detect orientation and acceleration. Stimulation of the utricular otoliths during head movement results in a behavioral response called the vestibulo-ocular reflex (VOR). The VOR is a compensatory eye rotation that serves to maintain a stable image during movement. VOR is characterized by gain (ratio of eye amplitude to head amplitude) and phase shift (temporal synchrony). We hypothesized that elevated pCO2 would increase OS and affect the VOR. We found that the sagittae and lapilli of young larvae reared at 2500 µatm pCO2 (treatment) were 14 to 20% and 37 to 39% larger in area, respectively, than those of larvae reared at 400 µatm pCO2 (control). The mean gain of treatment larvae (0.39 +/- 0.05, n = 28) was not statistically different from that of control larvae (0.30 +/- 0.03, n = 20), although there was a tendency for treatment larvae to have a larger gain. Phase shift was unchanged. Our lack of detection of a significant effect of elevated pCO2 on the VOR may be a result of the low turbulence conditions of the experiments, large natural variation in otolith size, calibration of the VOR or mechanism of acid?base regulation of white seabass larvae.

Keyword(s):

Animalia; Atractoscion nobilis; Behaviour; Bottles or small containers/Aquaria (<20 L); Chordata; Coast and continental shelf; Growth/Morphology; Laboratory experiment; Nekton; North Pacific; Pelagos; Single species; Temperate

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2016-12-20.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Shen, Sara G		study
2	Species	Species		Shen, Sara G
3	Registration number of species	Reg spec no		Shen, Sara G
4	Uniform resource locator/link to reference	URL ref		Shen, Sara G		WoRMS Aphia ID
5	Experiment	Exp		Shen, Sara G
6	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Shen, Sara G		treatment
7	Replicates	Repl	#	Shen, Sara G
8	Otolith area	Otolith area	mm²	Shen, Sara G		sagittae
9	Otolith area, standard error	Otolith area std e	±	Shen, Sara G		sagittae
10	Otolith area	Otolith area	mm²	Shen, Sara G		lapilli
11	Otolith area, standard error	Otolith area std e	±	Shen, Sara G		lapilli
12	Gain	Gain		Shen, Sara G		ratio of eye amplitude to head amplitude
13	Gain, standard error	Gain std e	±	Shen, Sara G		ratio of eye amplitude to head amplitude
14	Phase shift	Phase shift		Shen, Sara G		temporal synchrony
15	Phase shift, standard error	Phase shift std e	±	Shen, Sara G		temporal synchrony
16	Salinity	Sal		Shen, Sara G
17	Temperature, water	Temp	°C	Shen, Sara G
18	Alkalinity, total	AT	µmol/kg	Shen, Sara G	Potentiometric titration
19	Carbon, inorganic, dissolved	DIC	µmol/kg	Shen, Sara G	Coulometric titration
20	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Shen, Sara G	Calculated using CO2calc
21	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
22	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
23	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
24	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
25	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
26	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
27	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
28	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
29	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

334 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Exp	6 pCO2water_SST_wet [µatm] (treatment)	7 Repl [#]	8 Otolith area [mm²] (sagittae)	9 Otolith area std e [±] (sagittae)	10 Otolith area [mm²] (lapilli)	11 Otolith area std e [±] (lapilli)	12 Gain (ratio of eye amplitude to hea...)	13 Gain std e [±] (ratio of eye amplitude to hea...)	14 Phase shift (temporal synchrony)	15 Phase shift std e [±] (temporal synchrony)	16 Sal	17 Temp [°C]	18 AT [µmol/kg] (Potentiometric titration)	19 DIC [µmol/kg] (Coulometric titration)	20 pCO2water_SST_wet [µatm] (Calculated using CO2calc)	21 CSC flag (Calculated using seacarb afte...)	22 pH (total scale, Calculated using...)	23 CO2 [µmol/kg] (Calculated using seacarb afte...)	24 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	25 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	26 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	27 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	28 Omega Arg (Calculated using seacarb afte...)	29 Omega Cal (Calculated using seacarb afte...)
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OS1	400	13	0.000715	0.000019							33.61	18.0	2245	2025	416	15	8.03	14.28	413.34	414.79	1851.41	159.30	2.48	3.84
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OS1	2500	12	0.000982	0.000017							33.60	18.0	2242	2274	2468	15	7.32	85.07	2462.23	2470.84	2152.77	36.15	0.56	0.87
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OS2	400	18	0.000762	0.000019							33.68	18.0	2246	2030	426	15	8.02	14.64	423.85	425.33	1858.58	156.78	2.44	3.78
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OS2	2500	20	0.001061	0.000017							33.65	18.0	2248	2279	2465	15	7.32	84.62	2449.78	2458.35	2157.84	36.54	0.57	0.88
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OS1	400	15			0.001454	0.000018					33.61	18.0	2245	2025	416	15	8.03	14.28	413.34	414.79	1851.41	159.30	2.48	3.84
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OS1	2500	15			0.001663	0.000018					33.60	18.0	2242	2274	2468	15	7.32	85.07	2462.23	2470.84	2152.77	36.15	0.56	0.87
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OS2	400	15			0.001609	0.000027					33.68	18.0	2246	2030	426	15	8.02	14.64	423.85	425.33	1858.58	156.78	2.44	3.78
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OS2	2500	16			0.001936	0.000029					33.65	18.0	2248	2279	2465	15	7.32	84.62	2449.78	2458.35	2157.84	36.54	0.57	0.88
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	VOR 1	400	5					0.3	0.09	7.4	9.3	33.61	18.1	2233	2029	450	15	7.99	15.51	450.07	451.64	1864.46	149.03	2.32	3.59
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	VOR 2	400	6					0.3	0.04	-10.8	4.4	33.62	18.0	2256	2054	466	15	7.99	16.05	464.68	466.30	1890.63	147.82	2.30	3.56
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	VOR 3	400	9					0.3	0.05	-7.6	8.2	33.62	18.0	2249	2045	455	15	7.99	15.69	454.23	455.82	1879.83	149.48	2.33	3.60
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	VOR 1	2500	5					0.37	0.13	-3.0	11.4	33.57	18.0	2239	2258	2230	15	7.36	77.02	2228.65	2236.45	2141.49	39.50	0.61	0.95
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	VOR 2	2500	9					0.39	0.10	1.6	5.4	33.63	18.0	2254	2282	2407	15	7.33	82.88	2399.05	2407.45	2161.69	37.43	0.58	0.90
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	VOR 3	2500	14					0.4	0.05	-6.1	9.0	33.65	18.0	2251	2282	2472	15	7.32	85.06	2465.99	2474.61	2160.91	36.52	0.57	0.88