Citation:

s00360-016-0971-7

Name: Hyperventilation and blood acid-base balance in hypercapnia exposed red drum (Sciaenops ocellatus)
Published: 2016-12-15
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Animalia; Behaviour; Chordata; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Laboratory experiment; Nekton; North Atlantic; Pelagos; Sciaenops ocellatus; Single species; Temperate

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Abstract:

Hyperventilation is a common response in fish exposed to elevated water CO2. It is believed to lessen the respiratory acidosis associated with hypercapnia by lowering arterial PCO2, but the contribution of hyperventilation to blood acid-base compensation has yet to be quantified. Hyperventilation may also increase the flux of irons across the gill epithelium and the cost of osmoregulation, owing to the osmo-respiratory compromise. Therefore, hypercapnia exposed fish may increase standard metabolic rate (SMR) leaving less energy for physiological functions such as foraging, migration, growth and reproduction. Here we show that gill ventilation, blood PCO2 and total blood [CO2] increased in red drum (Sciaenops ocellatus) exposed to 1000 and 5000 µatm water CO2, and that blood PCO2 and total blood [CO2] decrease in fish during hypoxia induced hyperventilation. Based on these results we estimate the ventilatory contributions to total acid-base compensation in 1000 and 5000 µatm water CO2. We find that S. ocellatus only utilize a portion of its ventilatory capacity to reduce the acid-base disturbance in 1000 µatm water CO2. SMR was unaffected by both salinity and hypercapnia exposure indicating that the cost of osmoregulation is small relative to SMR, and that the lack of increased ventilation in 1000 µatm water CO2 despite the capacity to do so is not due to an energetic tradeoff between acid-base balance and osmoregulation. Therefore, while ocean acidification may impact ventilatory parameters, there will be little impact on the overall energy budget of S. ocellatus.

Keyword(s):

Animalia; Behaviour; Chordata; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Laboratory experiment; Nekton; North Atlantic; Pelagos; Sciaenops ocellatus; Single species; Temperate

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2016-12-13.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Ern, Rasmus		study
2	Species	Species		Ern, Rasmus
3	Registration number of species	Reg spec no		Ern, Rasmus
4	Uniform resource locator/link to reference	URL ref		Ern, Rasmus		WoRMS Aphia ID
5	Treatment	Treat		Ern, Rasmus
6	Time in hours	Time	h	Ern, Rasmus
7	Ventilation frequency	Vf	#/min	Ern, Rasmus
8	Ventilation frequency, standard error	Vf std e	±	Ern, Rasmus
9	Ventilation amplitude, relative	Vamp		Ern, Rasmus
10	Ventilation amplitude, relative, standard error	Vamp std e	±	Ern, Rasmus
11	Ventilation stroke volume, relative	Vsv		Ern, Rasmus
12	Ventilation stroke volume, relative, standard error	Vsv std e	±	Ern, Rasmus
13	Ventilatory minute volume, relative	Vmv		Ern, Rasmus
14	Ventilatory minute volume, relative, standard error	Vmv std e	±	Ern, Rasmus
15	pH	pH		Ern, Rasmus		blood
16	pH, standard error	pH std e	±	Ern, Rasmus		blood
17	Carbon dioxide, partial pressure, blood	pCO2 blood	mm Hg	Ern, Rasmus
18	Carbon dioxide, partial pressure, blood, standard error	pCO2 blood std e	±	Ern, Rasmus
19	Carbon dioxide	CO2	µmol/l	Ern, Rasmus		blood
20	Carbon dioxide, standard error	CO2 std e	±	Ern, Rasmus		blood
21	Salinity	Sal		Ern, Rasmus
22	Temperature, water	Temp	°C	Ern, Rasmus
23	Temperature, water, standard error	T std e	±	Ern, Rasmus
24	pH	pH		Ern, Rasmus	Potentiometric	NBS scale
25	pH, standard error	pH std e	±	Ern, Rasmus	Potentiometric	NBS scale
26	Alkalinity, total	AT	µmol/kg	Ern, Rasmus	Potentiometric titration
27	Alkalinity, total, standard error	AT std e	±	Ern, Rasmus	Potentiometric titration
28	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Ern, Rasmus	Calculated using CO2SYS
29	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error	pCO2water_SST_wet std e	±	Ern, Rasmus	Calculated using CO2SYS
30	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
31	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
32	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
33	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
34	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
35	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
36	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
37	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
38	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
39	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

518 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Treat	6 Time [h]	7 Vf [#/min]	8 Vf std e [±]	9 Vamp	10 Vamp std e [±]	11 Vsv	12 Vsv std e [±]	13 Vmv	14 Vmv std e [±]	15 pH (blood)	16 pH std e [±] (blood)	17 pCO2 blood [mm Hg]	18 pCO2 blood std e [±]	19 CO2 [µmol/l] (blood)	20 CO2 std e [±] (blood)	21 Sal	22 Temp [°C]	23 T std e [±]	24 pH (NBS scale, Potentiometric)	25 pH std e [±] (NBS scale, Potentiometric)	26 AT [µmol/kg] (Potentiometric titration)	27 AT std e [±] (Potentiometric titration)	28 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	29 pCO2water_SST_wet std e [±] (Calculated using CO2SYS)	30 CSC flag (Calculated using seacarb afte...)	31 pH (total scale, Calculated using...)	32 CO2 [µmol/kg] (Calculated using seacarb afte...)	33 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	34 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	35 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	36 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	37 DIC [µmol/kg] (Calculated using seacarb afte...)	38 Omega Arg (Calculated using seacarb afte...)	39 Omega Cal (Calculated using seacarb afte...)
laboratory	Sciaenops ocellatus (fish)	159335	marinespecies.org	pCO2=380 µatm		30.3	3.2	3.1	0.8	3.2	0.7	95	24							35	22	1	8.08	0.03	2421	13	567	40	26	7.95	16.72	544.67	546.48	1999.51	172.89	2189.12	2.71	4.15
laboratory	Sciaenops ocellatus (fish)	159335	marinespecies.org	pCO2=380 µatm	4	30.8	3.4	3.2	0.8	3.2	0.5	98	22							35	22	1	8.08	0.03	2421	13	567	40	26	7.95	16.72	544.67	546.48	1999.51	172.89	2189.12	2.71	4.15
laboratory	Sciaenops ocellatus (fish)	159335	marinespecies.org	pCO2=380 µatm	24	32.5	3.8	2.9	0.7	2.9	0.4	93	21							35	22	1	8.08	0.03	2421	13	567	40	26	7.95	16.72	544.67	546.48	1999.51	172.89	2189.12	2.71	4.15
laboratory	Sciaenops ocellatus (fish)	159335	marinespecies.org	pCO2=380 µatm		31.9	4.3	2.8	0.5	3.1	0.5	93	16							35	22	1	8.08	0.03	2421	13	567	40	26	7.95	16.72	544.67	546.48	1999.51	172.89	2189.12	2.71	4.15
laboratory	Sciaenops ocellatus (fish)	159335	marinespecies.org	pCO2=1000 µatm	4	37.3	5.9	3.0	0.4	2.9	0.3	101	15							35	22	1	7.81	0.01	2445	18	1124	29	26	7.68	34.21	1114.62	1118.33	2197.04	102.00	2333.25	1.60	2.45
laboratory	Sciaenops ocellatus (fish)	159335	marinespecies.org	pCO2=1000 µatm	24	32.8	5.0	3.4	0.5	3.6	0.3	114	18							35	22	1	7.81	0.01	2445	18	1124	29	26	7.68	34.21	1114.62	1118.33	2197.04	102.00	2333.25	1.60	2.45
laboratory	Sciaenops ocellatus (fish)	159335	marinespecies.org	pCO2=380 µatm		31.1	3.6	1.7	0.3	1.8	0.3	52	9							35	22	1	8.08	0.03	2421	13	567	40	26	7.95	16.72	544.67	546.48	1999.51	172.89	2189.12	2.71	4.15
laboratory	Sciaenops ocellatus (fish)	159335	marinespecies.org	pCO2=5000 µatm	4	35.6	3.0	2.8	0.7	2.8	0.9	87	21							35	22	1	7.22	0.01	2855	103	5426	152	26	7.09	168.35	5485.25	5503.57	2776.75	33.11	2978.21	0.52	0.79
laboratory	Sciaenops ocellatus (fish)	159335	marinespecies.org	pCO2=5000 µatm	24	34.3	3.0	3.0	0.8	2.9	0.7	95	24							35	22	1	7.22	0.01	2855	103	5426	152	26	7.09	168.35	5485.25	5503.57	2776.75	33.11	2978.21	0.52	0.79
laboratory	Sciaenops ocellatus (fish)	159335	marinespecies.org	Nomoxia		24.8	2.7	4.6	0.6	6.4	1.3	149	19
laboratory	Sciaenops ocellatus (fish)	159335	marinespecies.org	Hypoxia	1	35.0	4.6	6.8	1.1	6.4	0.7	519	34
laboratory	Sciaenops ocellatus (fish)	159335	marinespecies.org	Hypoxia	24	33.2	2.3	6.5	0.8	6.6	1.0	211	25
laboratory	Sciaenops ocellatus (fish)	159335	marinespecies.org	Nomoxia	48	23.3	1.6	5.4	0.8	7.6	1.1	175	24
laboratory	Sciaenops ocellatus (fish)	159335	marinespecies.org	pCO2=380 µatm										7.75	0.01	3.08	0.30	7460	800	35	22	1	8.08	0.03	2421	13	567	40	26	7.95	16.72	544.67	546.48	1999.51	172.89	2189.12	2.71	4.15
laboratory	Sciaenops ocellatus (fish)	159335	marinespecies.org	pCO2=1000 µatm	24									7.75	0.02	4.06	0.09	9780	680	35	22	1	7.81	0.01	2445	18	1124	29	26	7.68	34.21	1114.62	1118.33	2197.04	102.00	2333.25	1.60	2.45
laboratory	Sciaenops ocellatus (fish)	159335	marinespecies.org	pCO2=380 µatm										7.74	0.02	3.18	0.34	7340	680	35	22	1	8.08	0.03	2421	13	567	40	26	7.95	16.72	544.67	546.48	1999.51	172.89	2189.12	2.71	4.15
laboratory	Sciaenops ocellatus (fish)	159335	marinespecies.org	pCO2=5000 µatm	24									7.76	0.02	5.78	0.15	14140	730	35	22	1	7.22	0.01	2855	103	5426	152	26	7.09	168.35	5485.25	5503.57	2776.75	33.11	2978.21	0.52	0.79
laboratory	Sciaenops ocellatus (fish)	159335	marinespecies.org	Nomoxia										7.80	0.03	2.70	0.40	7020	600
laboratory	Sciaenops ocellatus (fish)	159335	marinespecies.org	Hypoxia	1									7.83	0.03	1.89	0.30	5370	560
laboratory	Sciaenops ocellatus (fish)	159335	marinespecies.org	Hypoxia	24									7.82	0.02	1.60	0.20	4520	320
laboratory	Sciaenops ocellatus (fish)	159335	marinespecies.org	Nomoxia	48									7.80	0.04	2.39	0.34	6390	620