Citation:

bg-13-6247-2016

Name: Physiological responses of coastal and oceanic diatoms to diurnal fluctuations in seawater carbonate chemistry under two CO2 concentrations
Published: 2016-11-24
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; North Atlantic; Ochrophyta; Other; Pelagos; Phytoplankton; Primary production/Photosynthesis; Respiration; Single species; Thalassiosira oceanica; Thalassiosira weissflogii

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Abstract:

Diel and seasonal fluctuations in seawater carbonate chemistry are common in coastal waters, while in the open-ocean carbonate chemistry is much less variable. In both of these environments, ongoing ocean acidification is being superimposed on the natural dynamics of the carbonate buffer system to influence the physiology of phytoplankton. Here, we show that a coastal Thalassiosira weissflogii isolate and an oceanic diatom, Thalassiosira oceanica, respond differentially to diurnal fluctuating carbonate chemistry in current and ocean acidification (OA) scenarios. A fluctuating carbonate chemistry regime showed positive or negligible effects on physiological performance of the coastal species. In contrast, the oceanic species was significantly negatively affected. The fluctuating regime reduced photosynthetic oxygen evolution rates and enhanced dark respiration rates of T. oceanica under ambient CO2 concentration, while in the OA scenario the fluctuating regime depressed its growth rate, chlorophyll a content, and elemental production rates. These contrasting physiological performances of coastal and oceanic diatoms indicate that they differ in the ability to cope with dynamic pCO2. We propose that, in addition to the ability to cope with light, nutrient, and predation pressure, the ability to acclimate to dynamic carbonate chemistry may act as one determinant of the spatial distribution of diatom species. Habitat-relevant diurnal changes in seawater carbonate chemistry can interact with OA to differentially affect diatoms in coastal and pelagic waters.

Keyword(s):

Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; North Atlantic; Ochrophyta; Other; Pelagos; Phytoplankton; Primary production/Photosynthesis; Respiration; Single species; Thalassiosira oceanica; Thalassiosira weissflogii

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2016-11-24.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Gao, Kunshan		study
2	Species	Species		Gao, Kunshan
3	Registration number of species	Reg spec no		Gao, Kunshan
4	Uniform resource locator/link to reference	URL ref		Gao, Kunshan		WoRMS Aphia ID
5	Figure	Fig		Gao, Kunshan
6	Table	Tab		Gao, Kunshan
7	Time in hours	Time	h	Gao, Kunshan		after the onset of light
8	Treatment	Treat		Gao, Kunshan
9	pH	pH		Gao, Kunshan		NBS scale
10	pH, standard deviation	pH std dev	±	Gao, Kunshan		NBS scale
11	Growth rate	µ	1/day	Gao, Kunshan
12	Growth rate, standard deviation	µ std dev	±	Gao, Kunshan
13	Chlorophyll a production per cell	Chl a prod/cell	pg/#/day	Gao, Kunshan
14	Chlorophyll a, production, standard deviation	Chl a prod	±	Gao, Kunshan
15	Particulate organic carbon production per cell	POC prod/cell	pmol/#/day	Gao, Kunshan
16	Particulate organic carbon, production, standard deviation	POC prod std dev	±	Gao, Kunshan
17	Production of particulate organic nitrogen	PON prod	pmol/#/day	Gao, Kunshan
18	Particulate organic nitrogen production, standard deviation	PON prod std dev	±	Gao, Kunshan
19	Biogenic silica production per cell	bSiO2 prod/cell	fmol/#/day	Gao, Kunshan
20	Biogenic silica production, standard deviation	bSiO2 prod std dev	±	Gao, Kunshan
21	Net photosynthesis rate, oxygen, per chlorophyll a	PN O2/Chl a	µmol/µg/h	Gao, Kunshan
22	Net photosynthesis rate, standard deviation	PN std dev	±	Gao, Kunshan		Chlorophyll a-normalized
23	Net photosynthesis rate, oxygen, per cell	PN O2/cell	fmol/#/h	Gao, Kunshan
24	Net photosynthesis rate, standard deviation	PN std dev	±	Gao, Kunshan		Cell-based
25	Respiration rate, oxygen, per cell	Resp O2/cell	fmol/#/h	Gao, Kunshan
26	Respiration rate, oxygen, standard deviation	Resp O2 std dev	±	Gao, Kunshan
27	Cell size	Cell size	µm	Gao, Kunshan
28	Cell size, standard deviation	Cell size std dev	±	Gao, Kunshan
29	Chlorophyll a per cell	Chl a/cell	pg/#	Gao, Kunshan
30	Chlorophyll a, standard deviation	Chl a std dev	±	Gao, Kunshan
31	Particulate organic carbon, per cell	POC/cell	pmol/#	Gao, Kunshan
32	Particulate organic carbon content per cell, standard deviation	POC cont/cell std dev	±	Gao, Kunshan
33	Particulate organic nitrogen per cell	PON/cell	pmol/#	Gao, Kunshan
34	Particulate organic nitrogen, standard deviation	PON std dev	±	Gao, Kunshan
35	Biogenic silica, per cell	bSiO2/cell	fmol/#	Gao, Kunshan
36	Biogenic silica, standard deviation	bSiO2 std dev	±	Gao, Kunshan
37	Carbon, organic, particulate/Nitrogen, organic, particulate ratio	POC/PON		Gao, Kunshan
38	Carbon, organic, particulate/Nitrogen, organic, particulate ratio, standard deviation	POC/PON std dev	±	Gao, Kunshan
39	Biogenic particulate silica/Carbon, organic, particulate	bPSi/POC		Gao, Kunshan
40	Biogenic particulate silica/Carbon, organic, particulate, standard deviation	bPSi/POC std dev	±	Gao, Kunshan
41	Respiration/net photosynthesis ratio	R/P		Gao, Kunshan
42	Respiration/net photosynthesis ratio, standard deviation	R/P std dev	±	Gao, Kunshan
43	Effective photochemical quantum yield	phi PS II		Gao, Kunshan
44	Effective photochemical quantum yield, standard deviation	phi PS II std dev	±	Gao, Kunshan
45	Non photochemical quenching	NPQ		Gao, Kunshan
46	Non photochemical quenching, standard deviation	NPQ std dev	±	Gao, Kunshan
47	Temperature, water	Temp	°C	Gao, Kunshan
48	Salinity	Sal		Gao, Kunshan
49	pH	pH		Gao, Kunshan	Potentiometric	After dilution, NBS scale
50	pH, standard deviation	pH std dev	±	Gao, Kunshan	Potentiometric	After dilution, NBS scale
51	Alkalinity, total	AT	µmol/kg	Gao, Kunshan	Potentiometric titration	After dilution
52	Alkalinity, total, standard deviation	AT std dev	±	Gao, Kunshan	Potentiometric titration	After dilution
53	Carbon, inorganic, dissolved	DIC	µmol/kg	Gao, Kunshan	Calculated using CO2SYS	After dilution
54	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Gao, Kunshan	Calculated using CO2SYS	After dilution
55	Bicarbonate ion	[HCO3]-	µmol/kg	Gao, Kunshan	Calculated using CO2SYS	After dilution
56	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Gao, Kunshan	Calculated using CO2SYS	After dilution
57	Carbonate ion	[CO3]2-	µmol/kg	Gao, Kunshan	Calculated using CO2SYS	After dilution
58	Carbonate ion, standard deviation	[CO3]2- std dev	±	Gao, Kunshan	Calculated using CO2SYS	After dilution
59	Carbon dioxide	CO2	µmol/kg	Gao, Kunshan	Calculated using CO2SYS	After dilution
60	Carbon dioxide, standard deviation	CO2 std dev	±	Gao, Kunshan	Calculated using CO2SYS	After dilution
61	pH	pH		Gao, Kunshan	Potentiometric	Before dilution, NBS scale
62	pH, standard deviation	pH std dev	±	Gao, Kunshan	Potentiometric	Before dilution, NBS scale
63	Alkalinity, total	AT	µmol/kg	Gao, Kunshan	Potentiometric titration	Before dilution
64	Alkalinity, total, standard deviation	AT std dev	±	Gao, Kunshan	Potentiometric titration	Before dilution
65	Carbon, inorganic, dissolved	DIC	µmol/kg	Gao, Kunshan	Calculated using CO2SYS	Before dilution
66	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Gao, Kunshan	Calculated using CO2SYS	Before dilution
67	Bicarbonate ion	[HCO3]-	µmol/kg	Gao, Kunshan	Calculated using CO2SYS	Before dilution
68	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Gao, Kunshan	Calculated using CO2SYS	Before dilution
69	Carbonate ion	[CO3]2-	µmol/kg	Gao, Kunshan	Calculated using CO2SYS	Before dilution
70	Carbonate ion, standard deviation	[CO3]2- std dev	±	Gao, Kunshan	Calculated using CO2SYS	Before dilution
71	Carbon dioxide	CO2	µmol/kg	Gao, Kunshan	Calculated using CO2SYS	Before dilution
72	Carbon dioxide, standard deviation	CO2 std dev	±	Gao, Kunshan	Calculated using CO2SYS	Before dilution
73	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
74	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	After dilution, total scale
75	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	After dilution
76	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	After dilution
77	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	After dilution
78	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	After dilution
79	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	After dilution
80	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	After dilution
81	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	After dilution
82	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	After dilution
83	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Before dilution, total scale
84	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Before dilution
85	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Before dilution
86	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Before dilution
87	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Before dilution
88	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Before dilution
89	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Before dilution
90	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Before dilution
91	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Before dilution

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1944 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Fig	6 Tab	7 Time [h] (after the onset of light)	8 Treat	9 pH (NBS scale)	10 pH std dev [±] (NBS scale)	11 µ [1/day]	12 µ std dev [±]	13 Chl a prod/cell [pg/#/day]	14 Chl a prod [±]	15 POC prod/cell [pmol/#/day]	16 POC prod std dev [±]	17 PON prod [pmol/#/day]	18 PON prod std dev [±]	19 bSiO2 prod/cell [fmol/#/day]	20 bSiO2 prod std dev [±]	21 PN O2/Chl a [µmol/µg/h]	22 PN std dev [±] (Chlorophyll a-normalized)	23 PN O2/cell [fmol/#/h]	24 PN std dev [±] (Cell-based)	25 Resp O2/cell [fmol/#/h]	26 Resp O2 std dev [±]	27 Cell size [µm]	28 Cell size std dev [±]	29 Chl a/cell [pg/#]	30 Chl a std dev [±]	31 POC/cell [pmol/#]	32 POC cont/cell std dev [±]	33 PON/cell [pmol/#]	34 PON std dev [±]	35 bSiO2/cell [fmol/#]	36 bSiO2 std dev [±]	37 POC/PON	38 POC/PON std dev [±]	39 bPSi/POC	40 bPSi/POC std dev [±]	41 R/P	42 R/P std dev [±]	43 phi PS II	44 phi PS II std dev [±]	45 NPQ	46 NPQ std dev [±]	47 Temp [°C]	48 Sal	49 pH (After dilution, NBS scale, Po...)	50 pH std dev [±] (After dilution, NBS scale, Po...)	51 AT [µmol/kg] (After dilution, Potentiometri...)	52 AT std dev [±] (After dilution, Potentiometri...)	53 DIC [µmol/kg] (After dilution, Calculated us...)	54 DIC std dev [±] (After dilution, Calculated us...)	55 [HCO3]- [µmol/kg] (After dilution, Calculated us...)	56 [HCO3]- std dev [±] (After dilution, Calculated us...)	57 [CO3]2- [µmol/kg] (After dilution, Calculated us...)	58 [CO3]2- std dev [±] (After dilution, Calculated us...)	59 CO2 [µmol/kg] (After dilution, Calculated us...)	60 CO2 std dev [±] (After dilution, Calculated us...)	61 pH (Before dilution, NBS scale, P...)	62 pH std dev [±] (Before dilution, NBS scale, P...)	63 AT [µmol/kg] (Before dilution, Potentiometr...)	64 AT std dev [±] (Before dilution, Potentiometr...)	65 DIC [µmol/kg] (Before dilution, Calculated u...)	66 DIC std dev [±] (Before dilution, Calculated u...)	67 [HCO3]- [µmol/kg] (Before dilution, Calculated u...)	68 [HCO3]- std dev [±] (Before dilution, Calculated u...)	69 [CO3]2- [µmol/kg] (Before dilution, Calculated u...)	70 [CO3]2- std dev [±] (Before dilution, Calculated u...)	71 CO2 [µmol/kg] (Before dilution, Calculated u...)	72 CO2 std dev [±] (Before dilution, Calculated u...)	73 CSC flag (Calculated using seacarb afte...)	74 pH (After dilution, total scale, ...)	75 CO2 [µmol/kg] (After dilution, Calculated us...)	76 fCO2water_SST_wet [µatm] (After dilution, Calculated us...)	77 pCO2water_SST_wet [µatm] (After dilution, Calculated us...)	78 [HCO3]- [µmol/kg] (After dilution, Calculated us...)	79 [CO3]2- [µmol/kg] (After dilution, Calculated us...)	80 DIC [µmol/kg] (After dilution, Calculated us...)	81 Omega Arg (After dilution, Calculated us...)	82 Omega Cal (After dilution, Calculated us...)	83 pH (Before dilution, total scale,...)	84 CO2 [µmol/kg] (Before dilution, Calculated u...)	85 fCO2water_SST_wet [µatm] (Before dilution, Calculated u...)	86 pCO2water_SST_wet [µatm] (Before dilution, Calculated u...)	87 [HCO3]- [µmol/kg] (Before dilution, Calculated u...)	88 [CO3]2- [µmol/kg] (Before dilution, Calculated u...)	89 DIC [µmol/kg] (Before dilution, Calculated u...)	90 Omega Arg (Before dilution, Calculated u...)	91 Omega Cal (Before dilution, Calculated u...)
laboratory				1		0.0	steady carbonate chemistry at ambient pCO2	8.12000	0.01732
laboratory				1		1.5	steady carbonate chemistry at ambient pCO2	8.11667	0.01528
laboratory				1		3.0	steady carbonate chemistry at ambient pCO2	8.13333	0.01528
laboratory				1		4.5	steady carbonate chemistry at ambient pCO2	8.12333	0.02517
laboratory				1		6.0	steady carbonate chemistry at ambient pCO2	8.12667	0.01528
laboratory				1		7.5	steady carbonate chemistry at ambient pCO2	8.13000	0.00000
laboratory				1		9.0	steady carbonate chemistry at ambient pCO2	8.12667	0.01528
laboratory				1		10.5	steady carbonate chemistry at ambient pCO2	8.12667	0.02309
laboratory				1		12.0	steady carbonate chemistry at ambient pCO2	8.12333	0.01155
laboratory				1		13.5	steady carbonate chemistry at ambient pCO2	8.13000	0.02000
laboratory				1		15.0	steady carbonate chemistry at ambient pCO2	8.13667	0.01155
laboratory				1		16.5	steady carbonate chemistry at ambient pCO2	8.12333	0.02082
laboratory				1		18.0	steady carbonate chemistry at ambient pCO2	8.13333	0.01155
laboratory				1		19.5	steady carbonate chemistry at ambient pCO2	8.11667	0.02082
laboratory				1		21.0	steady carbonate chemistry at ambient pCO2	8.12000	0.01000
laboratory				1		22.5	steady carbonate chemistry at ambient pCO2	8.11667	0.01528
laboratory				1		24.0	steady carbonate chemistry at ambient pCO2	8.13667	0.01528
laboratory				1		0.0	diurnally carbonate chemistry fluctuating around ambient pCO2 level	7.83667	0.01528
laboratory				1		1.5	diurnally carbonate chemistry fluctuating around ambient pCO2 level	7.95000	0.01732
laboratory				1		3.0	diurnally carbonate chemistry fluctuating around ambient pCO2 level	8.04667	0.01155
laboratory				1		4.5	diurnally carbonate chemistry fluctuating around ambient pCO2 level	8.11667	0.02082
laboratory				1		6.0	diurnally carbonate chemistry fluctuating around ambient pCO2 level	8.15333	0.01528
laboratory				1		7.5	diurnally carbonate chemistry fluctuating around ambient pCO2 level	8.22333	0.01155
laboratory				1		9.0	diurnally carbonate chemistry fluctuating around ambient pCO2 level	8.28667	0.01528
laboratory				1		10.5	diurnally carbonate chemistry fluctuating around ambient pCO2 level	8.32667	0.02517
laboratory				1		12.0	diurnally carbonate chemistry fluctuating around ambient pCO2 level	8.35333	0.01528
laboratory				1		13.5	diurnally carbonate chemistry fluctuating around ambient pCO2 level	8.24000	0.02000
laboratory				1		15.0	diurnally carbonate chemistry fluctuating around ambient pCO2 level	8.16667	0.00577
laboratory				1		16.5	diurnally carbonate chemistry fluctuating around ambient pCO2 level	8.10667	0.02082
laboratory				1		18.0	diurnally carbonate chemistry fluctuating around ambient pCO2 level	8.07333	0.02082
laboratory				1		19.5	diurnally carbonate chemistry fluctuating around ambient pCO2 level	8.06333	0.02517
laboratory				1		21.0	diurnally carbonate chemistry fluctuating around ambient pCO2 level	7.99000	0.03000
laboratory				1		22.5	diurnally carbonate chemistry fluctuating around ambient pCO2 level	7.89333	0.01528
laboratory				1		24.0	diurnally carbonate chemistry fluctuating around ambient pCO2 level	7.87000	0.02000
laboratory				1		0.0	steady carbonate chemistry at elevated pCO2	7.79333	0.01528
laboratory				1		1.5	steady carbonate chemistry at elevated pCO2	7.78667	0.00577
laboratory				1		3.0	steady carbonate chemistry at elevated pCO2	7.79667	0.00577
laboratory				1		4.5	steady carbonate chemistry at elevated pCO2	7.78667	0.00577
laboratory				1		6.0	steady carbonate chemistry at elevated pCO2	7.79333	0.00577
laboratory				1		7.5	steady carbonate chemistry at elevated pCO2	7.79667	0.01528
laboratory				1		9.0	steady carbonate chemistry at elevated pCO2	7.80000	0.01000
laboratory				1		10.5	steady carbonate chemistry at elevated pCO2	7.78667	0.00577
laboratory				1		12.0	steady carbonate chemistry at elevated pCO2	7.78667	0.01528
laboratory				1		13.5	steady carbonate chemistry at elevated pCO2	7.78667	0.02082
laboratory				1		15.0	steady carbonate chemistry at elevated pCO2	7.79000	0.01000
laboratory				1		16.5	steady carbonate chemistry at elevated pCO2	7.79333	0.01528
laboratory				1		18.0	steady carbonate chemistry at elevated pCO2	7.79000	0.01000
laboratory				1		19.5	steady carbonate chemistry at elevated pCO2	7.78667	0.00577
laboratory				1		21.0	steady carbonate chemistry at elevated pCO2	7.78333	0.01528
laboratory				1		22.5	steady carbonate chemistry at elevated pCO2	7.78333	0.00577
laboratory				1		24.0	steady carbonate chemistry at elevated pCO2	7.78000	0.01000
laboratory				1		0.0	diurnally carbonate chemistry fluctuating around elevated pCO2 level	7.53000	0.02000
laboratory				1		1.5	diurnally carbonate chemistry fluctuating around elevated pCO2 level	7.61667	0.01528
laboratory				1		3.0	diurnally carbonate chemistry fluctuating around elevated pCO2 level	7.70333	0.01528
laboratory				1		4.5	diurnally carbonate chemistry fluctuating around elevated pCO2 level	7.78667	0.01528
laboratory				1		6.0	diurnally carbonate chemistry fluctuating around elevated pCO2 level	7.82333	0.01155
laboratory				1		7.5	diurnally carbonate chemistry fluctuating around elevated pCO2 level	7.88000	0.01000
laboratory				1		9.0	diurnally carbonate chemistry fluctuating around elevated pCO2 level	7.94333	0.01155
laboratory				1		10.5	diurnally carbonate chemistry fluctuating around elevated pCO2 level	8.03667	0.02517
laboratory				1		12.0	diurnally carbonate chemistry fluctuating around elevated pCO2 level	8.06333	0.01528
laboratory				1		13.5	diurnally carbonate chemistry fluctuating around elevated pCO2 level	7.88667	0.02309
laboratory				1		15.0	diurnally carbonate chemistry fluctuating around elevated pCO2 level	7.74000	0.01000
laboratory				1		16.5	diurnally carbonate chemistry fluctuating around elevated pCO2 level	7.65667	0.02517
laboratory				1		18.0	diurnally carbonate chemistry fluctuating around elevated pCO2 level	7.62667	0.02082
laboratory				1		19.5	diurnally carbonate chemistry fluctuating around elevated pCO2 level	7.60667	0.02082
laboratory				1		21.0	diurnally carbonate chemistry fluctuating around elevated pCO2 level	7.57333	0.02082
laboratory				1		22.5	diurnally carbonate chemistry fluctuating around elevated pCO2 level	7.54667	0.01528
laboratory				1		24.0	diurnally carbonate chemistry fluctuating around elevated pCO2 level	7.54333	0.01528
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	2, 3, 5			steady carbonate chemistry at ambient pCO2			0.96385	0.04584	3.12175	0.26829	6.68582	0.31790	1.16026	0.08810	1070.22	64.28	0.48502	0.06367			134.80129	22.04952																					20	35	8.12	0.03	2397	7	2132	20	1929	27	188	8	16	1	8.13	0.02	2399	2	2133	7	1929	12	189	5	15	1	26	8.00	15.49	477.83	479.46	1968.57	175.06	2159.12	2.72	4.19	8.01	15.08	465.41	467.00	1962.08	178.55	2155.72	2.78	4.27
laboratory	Thalassiosira oceanica (phytoplankton)	345549	marinespecies.org	2, 3, 5			steady carbonate chemistry at ambient pCO2			0.85946	0.02253	0.25975	0.02158	1.28172	0.10074	0.21160	0.02670	303.89	16.26	0.54552	0.07405			43.31666	8.89632																					20	35	8.12	0.03	2397	7	2132	20	1929	27	188	8	16	1	8.13	0.02	2399	2	2133	7	1929	12	189	5	15	1	26	8.00	15.49	477.83	479.46	1968.57	175.06	2159.12	2.72	4.19	8.01	15.08	465.41	467.00	1962.08	178.55	2155.72	2.78	4.27
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	2, 3, 5			diurnally carbonate chemistry fluctuating around ambient pCO2 level			0.99235	0.02861	3.12701	0.12268	7.52454	0.20705	1.32881	0.09721	1049.48	27.48	0.52078	0.07741			134.37215	23.84921																					20	35	8.13	0.01	2398	2	2128	6	1922	10	191	4	15	1	8.14	0.02	2388	19	2116	21	1910	22	191	5	15	1	26	8.01	15.08	465.21	466.79	1961.24	178.48	2154.79	2.77	4.27	8.02	14.61	450.74	452.27	1944.51	181.08	2140.19	2.82	4.33
laboratory	Thalassiosira oceanica (phytoplankton)	345549	marinespecies.org	2, 3, 5			diurnally carbonate chemistry fluctuating around ambient pCO2 level			0.88732	0.04377	0.29445	0.01276	1.48928	0.11567	0.25450	0.01057	303.28	14.07	0.38908	0.06966			69.58273	1.75812																					20	35	8.13	0.01	2398	2	2128	6	1922	10	191	4	15	1	8.14	0.02	2388	19	2116	21	1910	22	191	5	15	1	26	8.01	15.08	465.21	466.79	1961.24	178.48	2154.79	2.77	4.27	8.02	14.61	450.74	452.27	1944.51	181.08	2140.19	2.82	4.33
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	2, 3, 5			steady carbonate chemistry at elevated pCO2			0.90459	0.01443	2.94107	0.01466	9.30511	0.40698	1.75826	0.12523	1073.24	90.32	0.49098	0.03884			209.09607	38.10203																					20	35	7.80	0.02	2392	5	2279	10	2144	12	98	3	37	1	7.79	0.02	2401	6	2287	7	2153	8	98	4	37	2	26	7.68	35.62	1099.05	1102.80	2166.51	92.19	2294.31	1.43	2.21	7.67	36.66	1131.38	1135.23	2179.48	90.63	2306.77	1.41	2.17
laboratory	Thalassiosira oceanica (phytoplankton)	345549	marinespecies.org	2, 3, 5			steady carbonate chemistry at elevated pCO2			0.71694	0.03531	0.27061	0.03809	1.70741	0.17710	0.34887	0.03890	230.23	15.33	0.41926	0.04313			45.26772	10.80559																					20	35	7.80	0.02	2392	5	2279	10	2144	12	98	3	37	1	7.79	0.02	2401	6	2287	7	2153	8	98	4	37	2	26	7.68	35.62	1099.05	1102.80	2166.51	92.19	2294.31	1.43	2.21	7.67	36.66	1131.38	1135.23	2179.48	90.63	2306.77	1.41	2.17
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	2, 3, 5			diurnally carbonate chemistry fluctuating around elevated pCO2 level			0.95902	0.02572	3.13771	0.14918	9.86148	0.04228	1.72596	0.06446	996.30	32.64	0.51793	0.05081			212.41331	41.53931																					20	35	7.80	0.02	2406	13	2288	19	2152	20	100	3	36	2	7.82	0.01	2408	9	2283	12	2144	13	104	2	34	1	26	7.68	35.83	1105.57	1109.34	2179.40	92.74	2307.97	1.44	2.22	7.70	34.09	1052.01	1055.59	2171.59	96.76	2302.45	1.50	2.31
laboratory	Thalassiosira oceanica (phytoplankton)	345549	marinespecies.org	2, 3, 5			diurnally carbonate chemistry fluctuating around elevated pCO2 level			0.65042	0.01913	0.18929	0.01902	1.42985	0.00283	0.28403	0.02052	187.83	1.48	0.47189	0.01676			53.50115	6.88834																					20	35	7.80	0.02	2406	13	2288	19	2152	20	100	3	36	2	7.82	0.01	2408	9	2283	12	2144	13	104	2	34	1	26	7.68	35.83	1105.57	1109.34	2179.40	92.74	2307.97	1.44	2.22	7.70	34.09	1052.01	1055.59	2171.59	96.76	2302.45	1.50	2.31
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		0.5	steady carbonate chemistry at ambient pCO2															1276.54408000	17.113470000
laboratory	Thalassiosira oceanica (phytoplankton)	345549	marinespecies.org	4		0.5	steady carbonate chemistry at ambient pCO2															101.03020000	12.737190000
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		0.5	diurnally carbonate chemistry fluctuating around ambient pCO2 level															1287.90418000	79.802100000
laboratory	Thalassiosira oceanica (phytoplankton)	345549	marinespecies.org	4		0.5	diurnally carbonate chemistry fluctuating around ambient pCO2 level															88.25559000	9.141930000
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		0.5	steady carbonate chemistry at elevated pCO2															1171.59995000	59.288350000
laboratory	Thalassiosira oceanica (phytoplankton)	345549	marinespecies.org	4		0.5	steady carbonate chemistry at elevated pCO2															84.50482000	17.032050000
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		0.5	diurnally carbonate chemistry fluctuating around elevated pCO2 level															1205.63955000	46.046930000
laboratory	Thalassiosira oceanica (phytoplankton)	345549	marinespecies.org	4		0.5	diurnally carbonate chemistry fluctuating around elevated pCO2 level															51.47934533	9.653289679
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		6.0	steady carbonate chemistry at ambient pCO2															1762.36801000	36.366970000
laboratory	Thalassiosira oceanica (phytoplankton)	345549	marinespecies.org	4		6.0	steady carbonate chemistry at ambient pCO2															164.10985000	16.934880000
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		6.0	diurnally carbonate chemistry fluctuating around ambient pCO2 level															1640.06210000	89.772590000
laboratory	Thalassiosira oceanica (phytoplankton)	345549	marinespecies.org	4		6.0	diurnally carbonate chemistry fluctuating around ambient pCO2 level															129.22444000	24.304770000
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		6.0	steady carbonate chemistry at elevated pCO2															1574.71986000	62.763790000
laboratory	Thalassiosira oceanica (phytoplankton)	345549	marinespecies.org	4		6.0	steady carbonate chemistry at elevated pCO2															157.55257000	17.498840000
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		6.0	diurnally carbonate chemistry fluctuating around elevated pCO2 level															1562.28348000	36.894240000
laboratory	Thalassiosira oceanica (phytoplankton)	345549	marinespecies.org	4		6.0	diurnally carbonate chemistry fluctuating around elevated pCO2 level															137.00192000	7.292830000
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		11.5	steady carbonate chemistry at ambient pCO2															1426.53806000	150.914940000
laboratory	Thalassiosira oceanica (phytoplankton)	345549	marinespecies.org	4		11.5	steady carbonate chemistry at ambient pCO2															113.37376000	5.212840000
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		11.5	diurnally carbonate chemistry fluctuating around ambient pCO2 level															1358.13185000	108.035620000
laboratory	Thalassiosira oceanica (phytoplankton)	345549	marinespecies.org	4		11.5	diurnally carbonate chemistry fluctuating around ambient pCO2 level															80.34855000	9.818270000
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		11.5	steady carbonate chemistry at elevated pCO2															1418.08082000	26.492070000
laboratory	Thalassiosira oceanica (phytoplankton)	345549	marinespecies.org	4		11.5	steady carbonate chemistry at elevated pCO2															133.24865000	19.954220000
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		11.5	diurnally carbonate chemistry fluctuating around elevated pCO2 level															1375.42293000	57.832980000
laboratory	Thalassiosira oceanica (phytoplankton)	345549	marinespecies.org	4		11.5	diurnally carbonate chemistry fluctuating around elevated pCO2 level															116.03861000	16.152730000
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org		2		steady carbonate chemistry at ambient pCO2																			12.17	0.05	3.24	0.14	6.94	0.36	1.21	0.14	1110	10	5.78	0.40	0.16	0.01	0.08	0.01					20	35	8.12	0.03	2397	7	2132	20	1929	27	188	8	16	1	8.13	0.02	2399	2	2133	7	1929	12	189	5	15	1	26	8.00	15.49	477.83	479.46	1968.57	175.06	2159.12	2.72	4.19	8.01	15.08	465.41	467.00	1962.08	178.55	2155.72	2.78	4.27
laboratory	Thalassiosira oceanica (phytoplankton)	345549	marinespecies.org		2		steady carbonate chemistry at ambient pCO2																			5.58	0.01	0.30	0.03	1.49	0.12	0.25	0.03	350	30	6.10	0.60	0.24	0.02	0.27	0.07					20	35	8.12	0.03	2397	7	2132	20	1929	27	188	8	16	1	8.13	0.02	2399	2	2133	7	1929	12	189	5	15	1	26	8.00	15.49	477.83	479.46	1968.57	175.06	2159.12	2.72	4.19	8.01	15.08	465.41	467.00	1962.08	178.55	2155.72	2.78	4.27
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org		2		diurnally carbonate chemistry fluctuating around ambient pCO2 level																			12.17	0.05	3.15	0.05	7.59	0.23	1.34	0.12	1060	40	5.68	0.32	0.14	0.01	0.08	0.02					20	35	8.13	0.01	2398	2	2128	6	1922	10	191	4	15	1	8.14	0.02	2388	19	2116	21	1910	22	191	5	15	1	26	8.01	15.08	465.21	466.79	1961.24	178.48	2154.79	2.77	4.27	8.02	14.61	450.74	452.27	1944.51	181.08	2140.19	2.82	4.33
laboratory	Thalassiosira oceanica (phytoplankton)	345549	marinespecies.org		2		diurnally carbonate chemistry fluctuating around ambient pCO2 level																			5.56	0.03	0.33	0.02	1.68	0.20	0.29	0.01	340	30	5.87	0.70	0.20	0.02	0.55	0.12					20	35	8.13	0.01	2398	2	2128	6	1922	10	191	4	15	1	8.14	0.02	2388	19	2116	21	1910	22	191	5	15	1	26	8.01	15.08	465.21	466.79	1961.24	178.48	2154.79	2.77	4.27	8.02	14.61	450.74	452.27	1944.51	181.08	2140.19	2.82	4.33
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org		2		steady carbonate chemistry at elevated pCO2																			12.20	0.04	3.25	0.05	10.28	0.29	1.94	0.11	1190	100	5.30	0.20	0.12	0.01	0.13	0.03					20	35	7.80	0.02	2392	5	2279	10	2144	12	98	3	37	1	7.79	0.02	2401	6	2287	7	2153	8	98	4	37	2	26	7.68	35.62	1099.05	1102.80	2166.51	92.19	2294.31	1.43	2.21	7.67	36.66	1131.38	1135.23	2179.48	90.63	2306.77	1.41	2.17
laboratory	Thalassiosira oceanica (phytoplankton)	345549	marinespecies.org		2		steady carbonate chemistry at elevated pCO2																			5.71	0.02	0.38	0.06	2.38	0.17	0.49	0.03	320	20	4.90	0.16	0.14	0.01	0.29	0.06					20	35	7.80	0.02	2392	5	2279	10	2144	12	98	3	37	1	7.79	0.02	2401	6	2287	7	2153	8	98	4	37	2	26	7.68	35.62	1099.05	1102.80	2166.51	92.19	2294.31	1.43	2.21	7.67	36.66	1131.38	1135.23	2179.48	90.63	2306.77	1.41	2.17
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org		2		diurnally carbonate chemistry fluctuating around elevated pCO2 level																			12.18	0.04	3.27	0.07	10.28	0.28	1.80	0.06	1040	40	5.72	0.24	0.10	0.01	0.14	0.03					20	35	7.80	0.02	2406	13	2288	19	2152	20	100	3	36	2	7.82	0.01	2408	9	2283	12	2144	13	104	2	34	1	26	7.68	35.83	1105.57	1109.34	2179.40	92.74	2307.97	1.44	2.22	7.70	34.09	1052.01	1055.59	2171.59	96.76	2302.45	1.50	2.31
laboratory	Thalassiosira oceanica (phytoplankton)	345549	marinespecies.org		2		diurnally carbonate chemistry fluctuating around elevated pCO2 level																			5.63	0.02	0.29	0.02	2.20	0.07	0.44	0.03	290	10	5.05	0.36	0.13	0.01	0.39	0.04					20	35	7.80	0.02	2406	13	2288	19	2152	20	100	3	36	2	7.82	0.01	2408	9	2283	12	2144	13	104	2	34	1	26	7.68	35.83	1105.57	1109.34	2179.40	92.74	2307.97	1.44	2.22	7.70	34.09	1052.01	1055.59	2171.59	96.76	2302.45	1.50	2.31
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org		3	0.5	steady carbonate chemistry at ambient pCO2																																			0.61	0.01	0.13	0.02
laboratory	Thalassiosira oceanica (phytoplankton)	345549	marinespecies.org		3	0.5	steady carbonate chemistry at ambient pCO2																																			0.57	0.03	0.10	0.02
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org		3	0.5	diurnally carbonate chemistry fluctuating around ambient pCO2 level																																			0.61	0.01	0.13	0.01
laboratory	Thalassiosira oceanica (phytoplankton)	345549	marinespecies.org		3	0.5	diurnally carbonate chemistry fluctuating around ambient pCO2 level																																			0.58	0.01	0.06	0.02
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org		3	0.5	steady carbonate chemistry at elevated pCO2																																			0.59	0.01	0.13	0.01
laboratory	Thalassiosira oceanica (phytoplankton)	345549	marinespecies.org		3	0.5	steady carbonate chemistry at elevated pCO2																																			0.59	0.06	0.08	0.05
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org		3	0.5	diurnally carbonate chemistry fluctuating around elevated pCO2 level																																			0.54	0.03	0.23	0.05
laboratory	Thalassiosira oceanica (phytoplankton)	345549	marinespecies.org		3	0.5	diurnally carbonate chemistry fluctuating around elevated pCO2 level																																			0.61	0.03	0.12	0.09
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org		3	6.0	steady carbonate chemistry at ambient pCO2																																			0.60	0.01	0.08	0.03
laboratory	Thalassiosira oceanica (phytoplankton)	345549	marinespecies.org		3	6.0	steady carbonate chemistry at ambient pCO2																																			0.57	0.01	0.13	0.02
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org		3	6.0	diurnally carbonate chemistry fluctuating around ambient pCO2 level																																			0.60	0.01	0.08	0.02
laboratory	Thalassiosira oceanica (phytoplankton)	345549	marinespecies.org		3	6.0	diurnally carbonate chemistry fluctuating around ambient pCO2 level																																			0.57	0.01	0.14	0.01
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org		3	6.0	steady carbonate chemistry at elevated pCO2																																			0.58	0.01	0.11	0.01
laboratory	Thalassiosira oceanica (phytoplankton)	345549	marinespecies.org		3	6.0	steady carbonate chemistry at elevated pCO2																																			0.54	0.01	0.18	0.02
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org		3	6.0	diurnally carbonate chemistry fluctuating around elevated pCO2 level																																			0.59	0.01	0.09	0.01
laboratory	Thalassiosira oceanica (phytoplankton)	345549	marinespecies.org		3	6.0	diurnally carbonate chemistry fluctuating around elevated pCO2 level																																			0.56	0.01	0.19	0.01
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org		3	11.5	steady carbonate chemistry at ambient pCO2																																			0.58	0.01	0.08	0.01
laboratory	Thalassiosira oceanica (phytoplankton)	345549	marinespecies.org		3	11.5	steady carbonate chemistry at ambient pCO2																																			0.61	0.03	0.09	0.02
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org		3	11.5	diurnally carbonate chemistry fluctuating around ambient pCO2 level																																			0.58	0.01	0.07	0.01
laboratory	Thalassiosira oceanica (phytoplankton)	345549	marinespecies.org		3	11.5	diurnally carbonate chemistry fluctuating around ambient pCO2 level																																			0.60	0.03	0.06	0.01
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org		3	11.5	steady carbonate chemistry at elevated pCO2																																			0.57	0.01	0.06	0.01
laboratory	Thalassiosira oceanica (phytoplankton)	345549	marinespecies.org		3	11.5	steady carbonate chemistry at elevated pCO2																																			0.57	0.01	0.06	0.01
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org		3	11.5	diurnally carbonate chemistry fluctuating around elevated pCO2 level																																			0.57	0.01	0.07	0.01
laboratory	Thalassiosira oceanica (phytoplankton)	345549	marinespecies.org		3	11.5	diurnally carbonate chemistry fluctuating around elevated pCO2 level																																			0.57	0.01	0.07	0.01