Citation:

rspb.2016.1669

Name: Coral pH regulation of the calcifying fluid is modulated by seawater dissolved inorganic carbon concentration
Published: 2017
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Acid-base regulation; Animalia; Benthic animals; Benthos; Calcification/Dissolution; Cnidaria; Containers and aquaria (20-1000 L or < 1 m**2); Laboratory experiment; Laboratory strains; Not applicable; Primary production/Photosynthesis; Respiration; Single species; Stylophora pistillata

Always quote citation above when using data! You can download the citation in several formats below.

RIS Citation BibTeX Citation Text Citation Facebook Twitter

Abstract:

Reef coral calcification depends on regulation of pH in the internal calcifying fluid in which the coral skeleton forms. However, little is known about calcifying fluid pH (pHCF) regulation, despite its importance in determining the response of corals to ocean acidification. Here, we investigate the impact of seawater dissolved inorganic carbon (DIC) concentration on calcifying fluid pH in the coral Stylophora pistillata in seawater with manipulated [DIC] and constant pH. Our results reveal that regulation of pHCF and calcification rates are sensitive to changes in seawater [DIC] in the light and dark. While part of this relationship can be explained by changes in rates of photosynthesis and respiration, our data point to the importance of seawater DIC in pH regulation of the coral's calcifying cells. Our findings contribute towards a mechanistic understanding of how and why coral calcification is sensitive to changes in seawater carbonate chemistry, which is needed for predicting effects of environmental change on coral reefs and for robust interpretations of isotopic paleoenvironmental records in coral skeletons.

Keyword(s):

Acid-base regulation; Animalia; Benthic animals; Benthos; Calcification/Dissolution; Cnidaria; Containers and aquaria (20-1000 L or < 1 m**2); Laboratory experiment; Laboratory strains; Not applicable; Primary production/Photosynthesis; Respiration; Single species; Stylophora pistillata

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.8. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2016-11-14.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Comeau, Steeve		study
2	Species	Species		Comeau, Steeve
3	Registration number of species	Reg spec no		Comeau, Steeve
4	Uniform resource locator/link to reference	URL ref		Comeau, Steeve		WoRMS Aphia ID
5	Figure	Fig		Comeau, Steeve
6	Treatment	Treat		Comeau, Steeve		varying dissolved inorganic carbon concentration [DIC] (800, 1500, 2200, 2900 µmol/kg, in the V low, Low, Amb, and High treatments respectively
7	Photosynthesis rate of oxygen, per symbiont cell	Photosynth O/symbiont	µmol/#/h	Comeau, Steeve		net
8	Net photosynthesis rate, oxygen, standard error	PN O2 std e	±	Comeau, Steeve		per symbiont cell
9	Photosynthesis rate of oxygen, per symbiont cell	Photosynth O/symbiont	µmol/#/h	Comeau, Steeve		gross
10	Gross photosynthesis rate, oxygen, standard error	PG O2 std e	±	Comeau, Steeve		per symbiont cell
11	Respiration rate, oxygen	Resp O2	µmol/cm²/h	Comeau, Steeve		dark
12	Respiration rate, oxygen, standard error	Resp O2 std e	±	Comeau, Steeve		dark
13	Respiration rate, oxygen	Resp O2	µmol/cm²/h	Comeau, Steeve		light
14	Respiration rate, oxygen, standard error	Resp O2 std e	±	Comeau, Steeve		light
15	Calcification rate of calcium carbonate	Calc rate CaCO3	mg/cm²/day	Comeau, Steeve		integrated
16	Calcification rate, standard error	Calc rate std e	±	Comeau, Steeve		integrated
17	Calcification rate of calcium carbonate	Calc rate CaCO3	µg/cm²/h	Comeau, Steeve		light
18	Calcification rate, standard error	Calc rate std e	±	Comeau, Steeve		light
19	Calcification rate of calcium carbonate	Calc rate CaCO3	µg/cm²/h	Comeau, Steeve		dark
20	Calcification rate, standard error	Calc rate std e	±	Comeau, Steeve		dark
21	Calcifying fluid, pH	CF pH		Comeau, Steeve		light, total scale
22	Calcifying fluid, pH, standard error	CF pH std e	±	Comeau, Steeve		light, total scale
23	Calcifying fluid, pH	CF pH		Comeau, Steeve		dark, total scale
24	Calcifying fluid, pH, standard error	CF pH std e	±	Comeau, Steeve		dark, total scale
25	pH	pH		Comeau, Steeve		callicoblastic cells, light, total scale
26	pH, standard error	pH std e	±	Comeau, Steeve		callicoblastic cells, light, total scale
27	pH	pH		Comeau, Steeve		callicoblastic cells, dark, total scale
28	pH, standard error	pH std e	±	Comeau, Steeve		callicoblastic cells, dark, total scale
29	pH	pH		Comeau, Steeve		seawater, total scale
30	pH, standard error	pH std e	±	Comeau, Steeve		seawater, total scale
31	Alkalinity, total	AT	µmol/kg	Comeau, Steeve
32	Alkalinity, total, standard error	AT std e	±	Comeau, Steeve
33	Salinity	Sal		Comeau, Steeve
34	Temperature, water	Temp	°C	Comeau, Steeve
35	Temperature, water, standard error	T std e	±	Comeau, Steeve
36	pH	pH		Comeau, Steeve		seawater, total scale
37	pH, standard error	pH std e	±	Comeau, Steeve		seawater, total scale
38	Alkalinity, total	AT	µmol/kg	Comeau, Steeve
39	Alkalinity, total, standard error	AT std e	±	Comeau, Steeve
40	Salinity	Sal		Comeau, Steeve
41	Temperature, water	Temp	°C	Comeau, Steeve
42	Temperature, water, standard error	T std e	±	Comeau, Steeve
43	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
44	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
45	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
46	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
47	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
48	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
49	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
50	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
51	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
52	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
53	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
54	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
55	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
56	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
57	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
58	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
59	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

639 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Fig	6 Treat (varying dissolved inorganic c...)	7 Photosynth O/symbiont [µmol/#/h] (net)	8 PN O2 std e [±] (per symbiont cell)	9 Photosynth O/symbiont [µmol/#/h] (gross)	10 PG O2 std e [±] (per symbiont cell)	11 Resp O2 [µmol/cm²/h] (dark)	12 Resp O2 std e [±] (dark)	13 Resp O2 [µmol/cm²/h] (light)	14 Resp O2 std e [±] (light)	15 Calc rate CaCO3 [mg/cm²/day] (integrated)	16 Calc rate std e [±] (integrated)	17 Calc rate CaCO3 [µg/cm²/h] (light)	18 Calc rate std e [±] (light)	19 Calc rate CaCO3 [µg/cm²/h] (dark)	20 Calc rate std e [±] (dark)	21 CF pH (light, total scale)	22 CF pH std e [±] (light, total scale)	23 CF pH (dark, total scale)	24 CF pH std e [±] (dark, total scale)	25 pH (callicoblastic cells, light, ...)	26 pH std e [±] (callicoblastic cells, light, ...)	27 pH (callicoblastic cells, dark, t...)	28 pH std e [±] (callicoblastic cells, dark, t...)	29 pH (seawater, total scale)	30 pH std e [±] (seawater, total scale)	31 AT [µmol/kg]	32 AT std e [±]	33 Sal	34 Temp [°C]	35 T std e [±]	36 pH (seawater, total scale)	37 pH std e [±] (seawater, total scale)	38 AT [µmol/kg]	39 AT std e [±]	40 Sal	41 Temp [°C]	42 T std e [±]	43 CSC flag	44 CO2 [µmol/kg]	45 fCO2water_SST_wet [µatm]	46 pCO2water_SST_wet [µatm]	47 [HCO3]- [µmol/kg]	48 [CO3]2- [µmol/kg]	49 DIC [µmol/kg]	50 Omega Arg	51 Omega Cal	52 CO2 [µmol/kg]	53 fCO2water_SST_wet [µatm]	54 pCO2water_SST_wet [µatm]	55 [HCO3]- [µmol/kg]	56 [CO3]2- [µmol/kg]	57 DIC [µmol/kg]	58 Omega Arg	59 Omega Cal
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	2	Very low	0.000000175	0.0000000331	0.000000645	0.000000069	0.83	0.09	1.05	0.06															7.93	0.01	995	42	37	25.03	0.04	7.92	0.01	1007	45	37	24.97	0.02	8	6.21	221.13	221.83	763.15	73.55	842.91	1.15	1.74	6.48	230.54	231.28	777.80	73.10	857.38	1.14	1.73
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	2	Low	0.000000328	0.0000000279	0.000000615	0.000000055	0.81	0.04	0.75	0.04															7.94	0.01	1776	43	37	24.99	0.03	7.95	0.01	1773	43	37	25.00	0.04	8	11.23	399.63	400.91	1411.67	139.02	1561.92	2.18	3.29	10.90	387.97	389.21	1402.30	141.37	1554.57	2.21	3.35
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	2	Amb	0.000000579	0.0000000605	0.000001050	0.000000055	0.56	0.01	0.97	0.05															7.89	0.01	2465	8	37	25.02	0.04	7.89	0.01	2457	6	37	24.96	0.04	8	18.11	644.87	646.93	2029.85	178.36	2226.32	2.79	4.22	18.08	642.73	644.79	2023.82	177.44	2219.33	2.78	4.20
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	2	High	0.000000703	0.0000001310	0.000001370	0.000000221	0.54	0.06	0.74	0.05															7.90	0.02	3287	27	37	25.03	0.03	7.91	0.02	3269	27	37	25.01	0.02	8	23.69	843.89	846.59	2718.05	244.48	2986.22	3.83	5.79	22.94	816.76	819.38	2692.25	247.62	2962.82	3.87	5.86
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	3	Very low									1.11	0.06	15.15	4.76	11.94	0.74									7.93	0.01	995	42	37	25.03	0.04	7.92	0.01	1007	45	37	24.97	0.02	8	6.21	221.13	221.83	763.15	73.55	842.91	1.15	1.74	6.48	230.54	231.28	777.80	73.10	857.38	1.14	1.73
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	3	Low									1.85	0.14	33.61	6.08	25.03	2.67									7.94	0.01	1776	43	37	24.99	0.03	7.95	0.01	1773	43	37	25.00	0.04	8	11.23	399.63	400.91	1411.67	139.02	1561.92	2.18	3.29	10.90	387.97	389.21	1402.30	141.37	1554.57	2.21	3.35
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	3	Amb									2.32	0.08	76.96	3.49	38.55	4.92									7.89	0.01	2465	8	37	25.02	0.04	7.89	0.01	2457	6	37	24.96	0.04	8	18.11	644.87	646.93	2029.85	178.36	2226.32	2.79	4.22	18.08	642.73	644.79	2023.82	177.44	2219.33	2.78	4.20
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	3	High									2.15	0.08	79.82	10.24	40.31	7.25									7.90	0.02	3287	27	37	25.03	0.03	7.91	0.02	3269	27	37	25.01	0.02	8	23.69	843.89	846.59	2718.05	244.48	2986.22	3.83	5.79	22.94	816.76	819.38	2692.25	247.62	2962.82	3.87	5.86
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	4	Very low															7.92	0.01	7.91	0.02					7.93	0.01	995	42	37	25.03	0.04	7.92	0.01	1007	45	37	24.97	0.02	8	6.21	221.13	221.83	763.15	73.55	842.91	1.15	1.74	6.48	230.54	231.28	777.80	73.10	857.38	1.14	1.73
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	4	Low															7.97	0.04	7.93	0.03					7.94	0.01	1776	43	37	24.99	0.03	7.95	0.01	1773	43	37	25.00	0.04	8	11.23	399.63	400.91	1411.67	139.02	1561.92	2.18	3.29	10.90	387.97	389.21	1402.30	141.37	1554.57	2.21	3.35
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	4	Amb															8.15	0.01	8.02	0.02					7.89	0.01	2465	8	37	25.02	0.04	7.89	0.01	2457	6	37	24.96	0.04	8	18.11	644.87	646.93	2029.85	178.36	2226.32	2.79	4.22	18.08	642.73	644.79	2023.82	177.44	2219.33	2.78	4.20
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	4	High															8.23	0.02	8.11	0.01					7.90	0.02	3287	27	37	25.03	0.03	7.91	0.02	3269	27	37	25.01	0.02	8	23.69	843.89	846.59	2718.05	244.48	2986.22	3.83	5.79	22.94	816.76	819.38	2692.25	247.62	2962.82	3.87	5.86
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	5	Very low																			7.24	0.01	7.26	0.02	7.93	0.01	995	42	37	25.03	0.04	7.92	0.01	1007	45	37	24.97	0.02	8	6.21	221.13	221.83	763.15	73.55	842.91	1.15	1.74	6.48	230.54	231.28	777.80	73.10	857.38	1.14	1.73
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	5	Amb																			7.36	0.02	7.40	0.03	7.89	0.01	2465	8	37	25.02	0.04	7.89	0.01	2457	6	37	24.96	0.04	8	18.11	644.87	646.93	2029.85	178.36	2226.32	2.79	4.22	18.08	642.73	644.79	2023.82	177.44	2219.33	2.78	4.20
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	5	High																			7.46	0.03	7.45	0.04	7.90	0.02	3287	27	37	25.03	0.03	7.91	0.02	3269	27	37	25.01	0.02	8	23.69	843.89	846.59	2718.05	244.48	2986.22	3.83	5.79	22.94	816.76	819.38	2692.25	247.62	2962.82	3.87	5.86