Citation:

gcb.13501

Name: Decreased photosynthesis and growth with reduced respiration in the model diatom Phaeodactylum tricornutum grown under elevated CO2 over 1800 generations
Published: 2017
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Not applicable; Ochrophyta; Pelagos; Phaeodactylum tricornutum; Phytoplankton; Primary production/Photosynthesis; Respiration; Single species

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Abstract:

Studies on the long-term responses of marine phytoplankton to ongoing ocean acidification (OA) are appearing rapidly in the literature. However, only a few of these have investigated diatoms, which is disproportionate to their contribution to global primary production. Here we show that a population of the model diatom Phaeodactylum tricornutum, after growing under elevated CO2 (1000 matm, HCL, pHT: 7.70) for 1860 generations, showed significant differences in photosynthesis and growth from a population maintained in ambient CO2 and then transferred to elevated CO2 for 20 generations (HC). The HCL population had lower mitochondrial respiration, than did the control population maintained in ambient CO2 (400 matm, LCL, pHT: 8.02) for 1860 generations. Although the cells had higher respiratory carbon loss within 20 generations under the elevated CO2, being consistent to previous findings, they down-regulated their respiration to sustain their growth in longer duration under the OA condition. Responses of phytoplankton to OA may depend on the timescale for which they are exposed due to fluctuations in physiological traits over time. This study provides the first evidence that populations of the model species, P. tricornutum, differ phenotypically from each other after having been grown for differing spans of time under OA conditions, suggesting that long-term changes should be measured to understand responses of primary producers to OA, especially in waters with diatom-dominated phytoplankton assemblages.

Keyword(s):

Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Not applicable; Ochrophyta; Pelagos; Phaeodactylum tricornutum; Phytoplankton; Primary production/Photosynthesis; Respiration; Single species

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.8. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2016-10-24.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Gao, Kunshan		study
2	Species	Species		Gao, Kunshan
3	Registration number of species	Reg spec no		Gao, Kunshan
4	Uniform resource locator/link to reference	URL ref		Gao, Kunshan		WoRMS Aphia ID
5	Figure	Fig		Gao, Kunshan
6	Treatment	Treat		Gao, Kunshan
7	Time in days	Time	days	Gao, Kunshan
8	Ratio	Ratio		Gao, Kunshan		growth rate ratio of HCL to LCL
9	Ratio, standard deviation	Ratio std dev	±	Gao, Kunshan		growth rate ratio of HCL to LCL
10	Ratio	Ratio		Gao, Kunshan		cell size ratio of HCL to LCL
11	Ratio, standard deviation	Ratio std dev	±	Gao, Kunshan		cell size ratio of HCL to LCL
12	Growth rate	µ	1/day	Gao, Kunshan
13	Growth rate, standard deviation	µ std dev	±	Gao, Kunshan
14	Cell size	Cell size	µm	Gao, Kunshan		mean
15	Cell size, standard deviation	Cell size std dev	±	Gao, Kunshan		mean
16	Chlorophyll a per cell	Chl a/cell	pg/#	Gao, Kunshan
17	Chlorophyll a, standard deviation	Chl a std dev	±	Gao, Kunshan
18	Carotenoids per cell	Carotenoids/cell	pg/#	Gao, Kunshan
19	Carotenoids, standard deviation	Carotenoids std dev	±	Gao, Kunshan
20	Respiration rate, oxygen, per chlorophyll a	Resp O2/chl a	µmol/µg/h	Gao, Kunshan		dark
21	Respiration rate, oxygen, standard deviation	Resp O2 std dev	±	Gao, Kunshan		dark
22	Respiration rate, oxygen, per cell	Resp O2/cell	fmol/#/h	Gao, Kunshan		dark
23	Respiration rate, oxygen, standard deviation	Resp O2 std dev	±	Gao, Kunshan		dark
24	Net photosynthesis rate, oxygen, per chlorophyll a	PN O2/Chl a	µmol/µg/h	Gao, Kunshan
25	Net photosynthesis rate, standard deviation	PN std dev	±	Gao, Kunshan
26	Net photosynthesis rate, oxygen, per cell	PN O2/cell	fmol/#/h	Gao, Kunshan
27	Net photosynthesis rate, standard deviation	PN std dev	±	Gao, Kunshan
28	Maximum photochemical quantum yield of photosystem II	Fv/Fm		Gao, Kunshan
29	Maximum photochemical quantum yield of photosystem II, standard deviation	Fv/Fm std dev	±	Gao, Kunshan
30	Effective photochemical quantum yield	phi PS II		Gao, Kunshan
31	Effective photochemical quantum yield, standard deviation	phi PS II std dev	±	Gao, Kunshan
32	Non photochemical quenching	NPQ		Gao, Kunshan
33	Non photochemical quenching, standard deviation	NPQ std dev	±	Gao, Kunshan
34	Electron transport rate efficiency	alpha		Gao, Kunshan
35	Electron transport rate efficiency, standard deviation	alpha std dev	±	Gao, Kunshan
36	Maximal electron transport rate, relative	rETR max		Gao, Kunshan
37	Maximal electron transport rate, relative, standard deviation	rETR max std dev	±	Gao, Kunshan
38	Light saturation point	Ik	µmol/m²/s	Gao, Kunshan
39	Light saturation point, standard deviation	Ik std dev	±	Gao, Kunshan
40	Relative photoinhibition ratio	Rel photoinhibit	%	Gao, Kunshan
41	Relative photoinhibition ratio, standard deviation	Rel photoinhibit std dev	±	Gao, Kunshan
42	Salinity	Sal		Gao, Kunshan
43	Temperature, water	Temp	°C	Gao, Kunshan
44	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Gao, Kunshan
45	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Gao, Kunshan
46	pH	pH		Gao, Kunshan		total scale
47	pH, standard deviation	pH std dev	±	Gao, Kunshan		total scale
48	Carbon, inorganic, dissolved	DIC	µmol/kg	Gao, Kunshan	Calculated using CO2SYS
49	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Gao, Kunshan	Calculated using CO2SYS
50	Bicarbonate ion	[HCO3]-	µmol/kg	Gao, Kunshan	Calculated using CO2SYS
51	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Gao, Kunshan	Calculated using CO2SYS
52	Carbonate ion	[CO3]2-	µmol/kg	Gao, Kunshan	Calculated using CO2SYS
53	Carbonate ion, standard deviation	[CO3]2- std dev	±	Gao, Kunshan	Calculated using CO2SYS
54	Carbon dioxide	CO2	µmol/kg	Gao, Kunshan	Calculated using CO2SYS
55	Carbon dioxide, standard deviation	CO2 std dev	±	Gao, Kunshan	Calculated using CO2SYS
56	Alkalinity, total	AT	µmol/kg	Gao, Kunshan	Calculated using CO2SYS
57	Alkalinity, total, standard deviation	AT std dev	±	Gao, Kunshan	Calculated using CO2SYS
58	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
59	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
60	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
61	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
62	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
63	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
64	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
65	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
66	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1247 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Fig	6 Treat	7 Time [days]	8 Ratio (growth rate ratio of HCL to LCL)	9 Ratio std dev [±] (growth rate ratio of HCL to LCL)	10 Ratio (cell size ratio of HCL to LCL)	11 Ratio std dev [±] (cell size ratio of HCL to LCL)	12 µ [1/day]	13 µ std dev [±]	14 Cell size [µm] (mean)	15 Cell size std dev [±] (mean)	16 Chl a/cell [pg/#]	17 Chl a std dev [±]	18 Carotenoids/cell [pg/#]	19 Carotenoids std dev [±]	20 Resp O2/chl a [µmol/µg/h] (dark)	21 Resp O2 std dev [±] (dark)	22 Resp O2/cell [fmol/#/h] (dark)	23 Resp O2 std dev [±] (dark)	24 PN O2/Chl a [µmol/µg/h]	25 PN std dev [±]	26 PN O2/cell [fmol/#/h]	27 PN std dev [±]	28 Fv/Fm	29 Fv/Fm std dev [±]	30 phi PS II	31 phi PS II std dev [±]	32 NPQ	33 NPQ std dev [±]	34 alpha	35 alpha std dev [±]	36 rETR max	37 rETR max std dev [±]	38 Ik [µmol/m²/s]	39 Ik std dev [±]	40 Rel photoinhibit [%]	41 Rel photoinhibit std dev [±]	42 Sal	43 Temp [°C]	44 pCO2water_SST_wet [µatm]	45 pCO2 std dev [±]	46 pH (total scale)	47 pH std dev [±] (total scale)	48 DIC [µmol/kg] (Calculated using CO2SYS)	49 DIC std dev [±] (Calculated using CO2SYS)	50 [HCO3]- [µmol/kg] (Calculated using CO2SYS)	51 [HCO3]- std dev [±] (Calculated using CO2SYS)	52 [CO3]2- [µmol/kg] (Calculated using CO2SYS)	53 [CO3]2- std dev [±] (Calculated using CO2SYS)	54 CO2 [µmol/kg] (Calculated using CO2SYS)	55 CO2 std dev [±] (Calculated using CO2SYS)	56 AT [µmol/kg] (Calculated using CO2SYS)	57 AT std dev [±] (Calculated using CO2SYS)	58 CSC flag (Calculated using seacarb afte...)	59 CO2 [µmol/kg] (Calculated using seacarb afte...)	60 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	61 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	62 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	63 DIC [µmol/kg] (Calculated using seacarb afte...)	64 AT [µmol/kg] (Calculated using seacarb afte...)	65 Omega Arg (Calculated using seacarb afte...)	66 Omega Cal (Calculated using seacarb afte...)
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		27	1.04332	0.02066
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		33	1.00677	0.00586
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		87	1.03331	0.00101
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		159	0.98301	0.03552
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		165	1.01833	0.04486
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		177	1.01439	0.00796
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		201	1.00640	0.01273
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		219	0.95276	0.02374
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		305	0.99812	0.01900
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		475	1.01463	0.01217
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		503	1.00649	0.00493
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		508	0.97877	0.03702
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		513	1.06156	0.02698
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		518	1.02218	0.01346
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		523	0.99916	0.02168
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		528	0.93747	0.01897
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		533	1.04210	0.00905
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		538	0.99128	0.00987
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		543	1.00355	0.01589
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		548	0.92830	0.00957
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		553	0.96647	0.00645
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		558	0.96455	0.00449
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		563	1.01580	0.00752
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		568	0.96991	0.01022
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		573	0.97482	0.00781
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		578	0.97013	0.00127
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		583	0.96235	0.00723
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		588	0.94812	0.00278
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		643	1.00417	0.00531
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		649	0.98768	0.00808
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		655	1.04005	0.03099
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		661	1.02402	0.02781
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		667	0.99337	0.01682
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		681	1.12828	0.00578
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		687	1.01688	0.00842
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		693	0.99911	0.00359
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		699	1.03595	0.03252
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		705	1.01084	0.03944
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		711	1.00739	0.01726
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		729	0.98449	0.02706
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		734	1.04007	0.03544
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		746	1.01140	0.00624
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		758	0.99806	0.01395
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		764	0.99863	0.00751
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		770	0.99780	0.00771
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		776	0.98236	0.02408
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		782	0.96735	0.00310
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		788	1.01337	0.01974
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		794	0.98138	0.00344
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		798	1.02301	0.00617
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		872	0.99489	0.00882
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		879	0.89714	0.01523
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		886	0.97967	0.04052
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		893	1.04229	0.00609
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		896	0.96559	0.00689
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		901	0.90476	0.01640
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		906	1.00717	0.02223
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		909	1.02770	0.03760
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		911	0.90457	0.02791
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		914	1.01848	0.02804
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		921	1.04592	0.01361
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		927	0.98143	0.05266
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		949	1.06853	0.10141
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		955	1.02659	0.01021
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		963	0.85645	0.00234
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		970	0.91204	0.01459
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		977	0.94522	0.03328
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		982	0.98087	0.06237
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		1009	0.91462	0.00747
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		1016	0.97345	0.00642
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		1023	0.92981	0.03307
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		1030	0.86614	0.01029
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		1034	0.89191	0.00730
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		1035	0.78458	0.06596
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		27			1.00969	0.00067
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		33			1.00164	0.00187
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		87			1.00485	0.00524
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		159			0.98877	0.01033
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		165			0.99991	0.01151
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		177			1.01424	0.01229
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		201			0.98347	0.03129
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		219			1.01658	0.02946
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		305			0.98845	0.00646
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		475			0.99778	0.01788
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		503			1.01091	0.01430
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		508			1.06619	0.02810
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		513			1.02415	0.02096
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		518			1.03461	0.02544
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		523			1.01551	0.01697
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		528			1.09509	0.03011
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		533			1.00436	0.01230
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		538			1.02376	0.00792
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		543			0.98791	0.01634
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		548			1.01194	0.00199
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		553			0.98908	0.01247
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		558			0.98390	0.00372
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		563			1.01362	0.00649
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		568			0.99417	0.01374
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		573			0.98699	0.00774
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		578			0.97874	0.00359
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		583			0.99230	0.01207
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		588			0.98203	0.00977
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		643			0.98850	0.00805
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		649			1.00977	0.00450
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		655			0.97881	0.00716
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		661			0.96586	0.00872
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		667			0.98285	0.00974
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		681			1.00431	0.00663
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		687			0.98348	0.00552
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		693			0.97672	0.00256
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		699			0.98442	0.01831
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		705			0.98730	0.04047
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		711			0.97482	0.00410
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		729			0.97801	0.00487
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		734			0.98416	0.00290
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		746			0.98067	0.00622
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		752			0.94409	0.03022
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		764			0.98623	0.00696
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		770			0.97313	0.00738
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		776			0.96168	0.00592
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		782			0.95956	0.00883
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		788			0.97838	0.01315
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		794			0.95972	0.00746
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		872			0.96125	0.00652
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		879			0.92848	0.00305
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		886			0.96482	0.00353
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		893			0.96618	0.01594
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		1009			0.97462	0.00631
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		1016			0.95545	0.01035
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		1023			0.95968	0.02166
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		1030			0.92780	0.00374
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		1034			0.96243	0.00337
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	1		1035			0.96509	0.00944
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	2, 3 and 4	LCL						1.37463	0.00433	4.86736	0.01300	0.26809	0.00615	0.09073	0.00178	0.03777	0.00956	10.10495	2.42026	0.56069	0.09922	149.99480	23.70979	0.670	0.011	0.600	0.004	0.044	0.029	0.305	0.013	122.5	2.3	402.8	23.5	11.4	1.9	35	20	413	12	8.02	0.02	1943	74	1750	63	179	12	13.4	0.4	2206	88	21	13.34	411.54	1793.49	168.71	1975.55	2212.94	2.62	4.04
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	2, 3 and 4	HC						1.39195	0.00850	4.87781	0.01607	0.22062	0.01497	0.08042	0.01108	0.10563	0.01315	23.17519	1.43641	0.72903	0.07537	160.10786	6.72337	0.672	0.005	0.618	0.002	0.048	0.008	0.331	0.015	122.1	0.5	369.3	17.2	16.9	1.3	35	20	997	15	7.70	0.01	2185	30	2050	27	102	4	32.2	0.5	2311	36	21	32.20	993.48	2072.26	93.30	2197.76	2302.15	1.45	2.23
laboratory	Phaeodactylum tricornutum (phytoplankton)	175584	marinespecies.org	2, 3 and 4	HCL						1.19061	0.01288	4.69778	0.03688	0.36116	0.04910	0.12705	0.01701	0.01626	0.00041	5.86467	0.70493	0.35755	0.06374	127.05025	5.18527	0.672	0.002	0.594	0.006	0.057	0.006	0.302	0.006	117.6	1.6	390.9	13.8	19.2	2.0	35	20	1013	12	7.70	0.01	2187	58	2054	53	101	5	32.7	0.3	2310	64	21	32.71	1009.43	2105.51	94.80	2233.03	2338.40	1.47	2.27