Citation:

srep25752

Name: Pteropod eggs released at high pCO2 lack resilience to ocean acidification
Published: 2016-10-10
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Animalia; Antarctic; Bottles or small containers/Aquaria (<20 L); Development; Laboratory experiment; Limacina helicina antarctica; Mollusca; Open ocean; Pelagos; Polar; Reproduction; Single species; Zooplankton

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Abstract:

The effects of ocean acidification (OA) on the early recruitment of pteropods in the Scotia Sea, was investigated considering the process of spawning, quality of the spawned eggs and their capacity to develop. Maternal OA stress was induced on female pteropods (Limacina helicina antarctica) through exposure to present day pCO2 conditions and two potential future OA states (750 matm and 1200 matm). The eggs spawned from these females, both before and during their exposure to OA, were incubated themselves in this same range of conditions (embryonic OA stress). Maternal OA stress resulted in eggs with lower carbon content, while embryonic OA stress retarded development. The combination of maternal and embryonic OA stress reduced the percentage of eggs successfully reaching organogenesis by 80%. We propose that OA stress not only affects the somatic tissue of pteropods but also the functioning of their gonads. Corresponding in-situ sampling found that post-larval L. helicina antarctica concentrated around 600 m depth, which is deeper than previously assumed. A deeper distribution makes their exposure to waters undersaturated for aragonite more likely in the near future given that these waters are predicted to shoal from depth over the coming decades.

Keyword(s):

Animalia; Antarctic; Bottles or small containers/Aquaria (<20 L); Development; Laboratory experiment; Limacina helicina antarctica; Mollusca; Open ocean; Pelagos; Polar; Reproduction; Single species; Zooplankton

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.8. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: -55.246000 * Longitude: -41.265000

Date/Time Start: 2013-11-01T00:00:00 * Date/Time End: 2013-11-30T00:00:00

Event(s):

Scotia_sea * Latitude: -55.246000 * Longitude: -41.265000 * Date/Time Start: 2013-11-01T00:00:00 * Date/Time End: 2013-11-30T00:00:00 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2016-09-12.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Manno, Clara
2	Species	Species		Manno, Clara
3	Registration number of species	Reg spec no		Manno, Clara
4	Uniform resource locator/link to reference	URL ref		Manno, Clara
5	Time point, descriptive	Time point		Manno, Clara
6	Treatment	Treat		Manno, Clara
7	Eggs	Eggs	#	Manno, Clara		per pteropod
8	Egg ribbons, per individual	Egg ribbons/ind	#	Manno, Clara
9	Egg size	Egg size	µm	Manno, Clara
10	Eggs, carbon content per egg	C/egg	µg/#	Manno, Clara		organic carbon
11	Egg stages	Egg	%	Manno, Clara		no develop
12	Egg stages	Egg	%	Manno, Clara		morula
13	Egg stages	Egg	%	Manno, Clara		blastula
14	Egg stages	Egg	%	Manno, Clara		gastrula
15	Egg stages	Egg	%	Manno, Clara		organogen
16	Temperature, water	Temp	°C	Manno, Clara
17	Temperature, standard deviation	T std dev	±	Manno, Clara
18	Salinity	Sal		Manno, Clara
19	Salinity, standard deviation	Sal std dev	±	Manno, Clara
20	pH	pH		Manno, Clara	Potentiometric	NBS scale
21	pH, standard deviation	pH std dev	±	Manno, Clara	Potentiometric	NBS scale
22	Alkalinity, total	AT	µmol/kg	Manno, Clara	Potentiometric titration
23	Alkalinity, total, standard deviation	AT std dev	±	Manno, Clara	Potentiometric titration
24	Carbon, inorganic, dissolved	DIC	µmol/kg	Manno, Clara	Potentiometric titration
25	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Manno, Clara	Potentiometric titration
26	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Manno, Clara	Calculated using CO2SYS
27	Carbon dioxide, partial pressure, standard deviation	pCO2 std dev	±	Manno, Clara	Calculated using CO2SYS
28	Aragonite saturation state	Omega Arg		Manno, Clara	Calculated using CO2SYS
29	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Manno, Clara	Calculated using CO2SYS
30	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
31	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
32	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
33	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
34	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
35	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
36	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
37	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
38	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1548 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type	2 Species	3 Reg spec no	4 URL ref	5 Time point	6 Treat	7 Eggs [#] (per pteropod)	8 Egg ribbons/ind [#]	9 Egg size [µm]	10 C/egg [µg/#] (organic carbon)	11 Egg [%] (no develop)	12 Egg [%] (morula)	13 Egg [%] (blastula)	14 Egg [%] (gastrula)	15 Egg [%] (organogen)	16 Temp [°C]	17 T std dev [±]	18 Sal	19 Sal std dev [±]	20 pH (NBS scale, Potentiometric)	21 pH std dev [±] (NBS scale, Potentiometric)	22 AT [µmol/kg] (Potentiometric titration)	23 AT std dev [±] (Potentiometric titration)	24 DIC [µmol/kg] (Potentiometric titration)	25 DIC std dev [±] (Potentiometric titration)	26 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	27 pCO2 std dev [±] (Calculated using CO2SYS)	28 Omega Arg (Calculated using CO2SYS)	29 Omega Arg std dev [±] (Calculated using CO2SYS)	30 CSC flag (Calculated using seacarb afte...)	31 pH (total scale, Calculated using...)	32 CO2 [µmol/kg] (Calculated using seacarb afte...)	33 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	34 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	35 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	36 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	37 Omega Arg (Calculated using seacarb afte...)	38 Omega Cal (Calculated using seacarb afte...)
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 0	acclim -1	1285	3.0	64	0.640
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 0	acclim -2	1274	2.0	65	0.511
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 0	acclim -3	1253	2.0	56	0.450
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 0	acclim -4	1280	3.0	63	0.550
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 0	acclim -5	1292	2.0	65	0.591
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 0	acclim -6	1259	2.0	59	0.484
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 0	acclim -7	1258	3.0	62	0.532
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 0	acclim -8	1278	2.0	63	0.482
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 0	acclim -9	1279	2.0	58	0.581
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 2	Control 1										1.9	0.52	34.1	0.02	8.1	0.02	2330.32	10.5	2218.31	17	375	14	1.42	0.21	15	8.00	26.00	442.42	444.33	2101.11	91.21	1.38	2.19
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 2	Control 2										1.9	0.52	34.1	0.02	8.1	0.02	2330.32	10.5	2218.31	17	375	14	1.42	0.21	15	8.00	26.00	442.42	444.33	2101.11	91.21	1.38	2.19
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 2	Control 3										1.9	0.52	34.1	0.02	8.1	0.02	2330.32	10.5	2218.31	17	375	14	1.42	0.21	15	8.00	26.00	442.42	444.33	2101.11	91.21	1.38	2.19
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 2	PH1-1	865	3.0	61	0.353						1.9	0.54	34.1	0.02	7.8	0.01	2330.32	8.9	2281.14	6	750	24	0.91	0.14	15	7.81	42.26	719.14	722.24	2178.56	60.32	0.91	1.45
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 2	PH1-2	896	2.0	64	0.421						1.9	0.54	34.1	0.02	7.8	0.01	2330.32	8.9	2281.14	6	750	24	0.91	0.14	15	7.81	42.26	719.14	722.24	2178.56	60.32	0.91	1.45
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 2	PH1-3	835	2.5	58	0.311						1.9	0.54	34.1	0.02	7.8	0.01	2330.32	8.9	2281.14	6	750	24	0.91	0.14	15	7.81	42.26	719.14	722.24	2178.56	60.32	0.91	1.45
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 2	PH2-1	600	2.0	63	0.369						1.9	0.54	34.1	0.02	7.6	0.02	2330.32	9.5	2299.01	11	1200	34	0.76	0.05	15	7.75	48.97	833.40	836.99	2197.10	52.94	0.80	1.27
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 2	PH2-2	580	3.0	67	0.332						1.9	0.54	34.1	0.02	7.6	0.02	2330.32	9.5	2299.01	11	1200	34	0.76	0.05	15	7.75	48.97	833.40	836.99	2197.10	52.94	0.80	1.27
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 2	PH2-3	560	2.0	58	0.410						1.9	0.54	34.1	0.02	7.6	0.02	2330.32	9.5	2299.01	11	1200	34	0.76	0.05	15	7.75	48.97	833.40	836.99	2197.10	52.94	0.80	1.27
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 5	Control 1										1.9	0.52	34.1	0.02	8.1	0.02	2330.32	10.5	2218.31	17	375	14	1.42	0.21	15	8.00	26.00	442.42	444.33	2101.11	91.21	1.38	2.19
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 5	Control 2										1.9	0.52	34.1	0.02	8.1	0.02	2330.32	10.5	2218.31	17	375	14	1.42	0.21	15	8.00	26.00	442.42	444.33	2101.11	91.21	1.38	2.19
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 5	Control 3										1.9	0.52	34.1	0.02	8.1	0.02	2330.32	10.5	2218.31	17	375	14	1.42	0.21	15	8.00	26.00	442.42	444.33	2101.11	91.21	1.38	2.19
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 5	PH1-1	759	4.0	53	0.226						1.9	0.54	34.1	0.02	7.8	0.01	2330.32	8.9	2281.14	6	750	24	0.91	0.14	15	7.81	42.26	719.14	722.24	2178.56	60.32	0.91	1.45
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 5	PH1-2	680	3.5	56	0.241						1.9	0.54	34.1	0.02	7.8	0.01	2330.32	8.9	2281.14	6	750	24	0.91	0.14	15	7.81	42.26	719.14	722.24	2178.56	60.32	0.91	1.45
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 5	PH1-3	710	4.0	51	0.199						1.9	0.54	34.1	0.02	7.8	0.01	2330.32	8.9	2281.14	6	750	24	0.91	0.14	15	7.81	42.26	719.14	722.24	2178.56	60.32	0.91	1.45
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 5	PH2-1	610	5.0	52	0.248						1.9	0.54	34.1	0.02	7.6	0.02	2330.32	9.5	2299.01	11	1200	34	0.76	0.05	15	7.75	48.97	833.40	836.99	2197.10	52.94	0.80	1.27
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 5	PH2-2	630	4.0	49	0.287						1.9	0.54	34.1	0.02	7.6	0.02	2330.32	9.5	2299.01	11	1200	34	0.76	0.05	15	7.75	48.97	833.40	836.99	2197.10	52.94	0.80	1.27
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 5	PH2-3	580	4.5	51	0.211						1.9	0.54	34.1	0.02	7.6	0.02	2330.32	9.5	2299.01	11	1200	34	0.76	0.05	15	7.75	48.97	833.40	836.99	2197.10	52.94	0.80	1.27
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 0 +72h	Control 1					1.00	3.50	0.00	3.22	92.28	1.9	0.52	34.1	0.02	8.1	0.02	2330.32	10.5	2218.31	17	375	14	1.42	0.21	15	8.00	26.00	442.42	444.33	2101.11	91.21	1.38	2.19
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 0 +72h	Control 2					0.70	2.35	0.00	4.13	92.82	1.9	0.52	34.1	0.02	8.1	0.02	2330.32	10.5	2218.31	17	375	14	1.42	0.21	15	8.00	26.00	442.42	444.33	2101.11	91.21	1.38	2.19
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 0 +72h	Control 3					1.20	3.02	0.00	2.45	93.33	1.9	0.52	34.1	0.02	8.1	0.02	2330.32	10.5	2218.31	17	375	14	1.42	0.21	15	8.00	26.00	442.42	444.33	2101.11	91.21	1.38	2.19
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 0 +72h	PH1-1					1.00	3.09	0.00	8.09	87.82	1.9	0.54	34.1	0.02	7.8	0.01	2330.32	8.9	2281.14	6	750	24	0.91	0.14	15	7.81	42.26	719.14	722.24	2178.56	60.32	0.91	1.45
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 0 +72h	PH1-2					1.50	2.26	0.00	8.23	88.01	1.9	0.54	34.1	0.02	7.8	0.01	2330.32	8.9	2281.14	6	750	24	0.91	0.14	15	7.81	42.26	719.14	722.24	2178.56	60.32	0.91	1.45
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 0 +72h	PH1-3					0.56	3.59	0.00	9.34	86.51	1.9	0.54	34.1	0.02	7.8	0.01	2330.32	8.9	2281.14	6	750	24	0.91	0.14	15	7.81	42.26	719.14	722.24	2178.56	60.32	0.91	1.45
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 0 +72h	PH2-1					4.23	6.54	4.02	23.19	62.02	1.9	0.54	34.1	0.02	7.6	0.02	2330.32	9.5	2299.01	11	1200	34	0.76	0.05	15	7.75	48.97	833.40	836.99	2197.10	52.94	0.80	1.27
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 0 +72h	PH2-2					3.41	7.01	3.13	21.00	65.45	1.9	0.54	34.1	0.02	7.6	0.02	2330.32	9.5	2299.01	11	1200	34	0.76	0.05	15	7.75	48.97	833.40	836.99	2197.10	52.94	0.80	1.27
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 0 +72h	PH2-3					4.01	7.54	2.55	27.21	58.69	1.9	0.54	34.1	0.02	7.6	0.02	2330.32	9.5	2299.01	11	1200	34	0.76	0.05	15	7.75	48.97	833.40	836.99	2197.10	52.94	0.80	1.27
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 2 +72h	Control 1										1.9	0.52	34.1	0.02	8.1	0.02	2330.32	10.5	2218.31	17	375	14	1.42	0.21	15	8.00	26.00	442.42	444.33	2101.11	91.21	1.38	2.19
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 2 +72h	Control 2										1.9	0.52	34.1	0.02	8.1	0.02	2330.32	10.5	2218.31	17	375	14	1.42	0.21	15	8.00	26.00	442.42	444.33	2101.11	91.21	1.38	2.19
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 2 +72h	Control 3										1.9	0.52	34.1	0.02	8.1	0.02	2330.32	10.5	2218.31	17	375	14	1.42	0.21	15	8.00	26.00	442.42	444.33	2101.11	91.21	1.38	2.19
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 2 +72h	PH1-1					4.00	6.35	0.00	30.00	59.65	1.9	0.54	34.1	0.02	7.8	0.01	2330.32	8.9	2281.14	6	750	24	0.91	0.14	15	7.81	42.26	719.14	722.24	2178.56	60.32	0.91	1.45
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 2 +72h	PH1-2					3.10	5.67	0.00	26.23	65.00	1.9	0.54	34.1	0.02	7.8	0.01	2330.32	8.9	2281.14	6	750	24	0.91	0.14	15	7.81	42.26	719.14	722.24	2178.56	60.32	0.91	1.45
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 2 +72h	PH1-3					4.67	6.00	0.00	34.12	55.21	1.9	0.54	34.1	0.02	7.8	0.01	2330.32	8.9	2281.14	6	750	24	0.91	0.14	15	7.81	42.26	719.14	722.24	2178.56	60.32	0.91	1.45
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 2 +72h	PH2-1					9.21	11.10	2.01	36.00	41.68	1.9	0.54	34.1	0.02	7.6	0.02	2330.32	9.5	2299.01	11	1200	34	0.76	0.05	15	7.75	48.97	833.40	836.99	2197.10	52.94	0.80	1.27
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 2 +72h	PH2-2					8.10	12.80	2.56	33.53	43.01	1.9	0.54	34.1	0.02	7.6	0.02	2330.32	9.5	2299.01	11	1200	34	0.76	0.05	15	7.75	48.97	833.40	836.99	2197.10	52.94	0.80	1.27
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 2 +72h	PH2-3					9.65	12.22	1.45	39.12	37.56	1.9	0.54	34.1	0.02	7.6	0.02	2330.32	9.5	2299.01	11	1200	34	0.76	0.05	15	7.75	48.97	833.40	836.99	2197.10	52.94	0.80	1.27
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 5 +72h	Control 1										1.9	0.52	34.1	0.02	8.1	0.02	2330.32	10.5	2218.31	17	375	14	1.42	0.21	15	8.00	26.00	442.42	444.33	2101.11	91.21	1.38	2.19
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 5 +72h	Control 2										1.9	0.52	34.1	0.02	8.1	0.02	2330.32	10.5	2218.31	17	375	14	1.42	0.21	15	8.00	26.00	442.42	444.33	2101.11	91.21	1.38	2.19
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 5 +72h	Control 3										1.9	0.52	34.1	0.02	8.1	0.02	2330.32	10.5	2218.31	17	375	14	1.42	0.21	15	8.00	26.00	442.42	444.33	2101.11	91.21	1.38	2.19
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 5 +72h	PH1-1					11.35	8.00	2.09	50.00	28.56	1.9	0.54	34.1	0.02	7.8	0.01	2330.32	8.9	2281.14	6	750	24	0.91	0.14	15	7.81	42.26	719.14	722.24	2178.56	60.32	0.91	1.45
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 5 +72h	PH1-2					12.23	7.23	2.78	54.23	23.53	1.9	0.54	34.1	0.02	7.8	0.01	2330.32	8.9	2281.14	6	750	24	0.91	0.14	15	7.81	42.26	719.14	722.24	2178.56	60.32	0.91	1.45
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 5 +72h	PH1-3					13.00	8.75	1.34	46.12	30.79	1.9	0.54	34.1	0.02	7.8	0.01	2330.32	8.9	2281.14	6	750	24	0.91	0.14	15	7.81	42.26	719.14	722.24	2178.56	60.32	0.91	1.45
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 5 +72h	PH2-1					21.34	16.12	2.33	46.21	14.00	1.9	0.54	34.1	0.02	7.6	0.02	2330.32	9.5	2299.01	11	1200	34	0.76	0.05	15	7.75	48.97	833.40	836.99	2197.10	52.94	0.80	1.27
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 5 +72h	PH2-2					20.15	15.80	2.56	48.09	13.40	1.9	0.54	34.1	0.02	7.6	0.02	2330.32	9.5	2299.01	11	1200	34	0.76	0.05	15	7.75	48.97	833.40	836.99	2197.10	52.94	0.80	1.27
laboratory	Limacina helicina antarctica (zooplankton)	719576	marinespecies.org	day 5 +72h	PH2-3					22.10	16.00	1.01	44.56	16.33	1.9	0.54	34.1	0.02	7.6	0.02	2330.32	9.5	2299.01	11	1200	34	0.76	0.05	15	7.75	48.97	833.40	836.99	2197.10	52.94	0.80	1.27