Rickert, Esther; Wahl, Martin; Link, Heike; Richter, Hannes; Pohnert, Georg (2016): Seasonal variations in surface metabolite composition of Fucus vesiculosus and Fucus serratus sampled at outer Kiel Fjord (August 2012 - July 2013). PANGAEA, https://doi.org/10.1594/PANGAEA.864960, Supplement to: Rickert, E et al. (2016): Seasonal Variations in Surface Metabolite Composition of Fucus vesiculosus and Fucus serratus from the Baltic Sea. PLoS ONE, 11(12), e0168196, https://doi.org/10.1371/journal.pone.0168196
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The chemical composition of surface associated metabolites of two Fucus species (Fucus vesiculosus and Fucus serratus) was analysed by means of gas chromatography-mass spectrometry (GC-MS) to describe temporal patterns in chemical surface composition.
Method: The two perennial brown macroalgae F. vesiculosus and F. serratus were sampled monthly at Bülk, outer Kiel Fjord, Germany (54°27'21 N / 10°11'57 E) over an entire year (August 2012 - July 2013). Per month and species six non-fertile Fucus individuals were collected from mixed stands at a depth of 0.5 m under mid water level. For surface extraction approx. 50 g of the upper 5-10 cm apical thalli tips were cut off per species. The surface extraction of Fucus was performed according to the protocol of de Nys and co-workers (1998) with minor modifications (see Rickert et al. 2015).
GC/EI-MS measurements were performed with a Waters GCT premier (Waters, Manchester, UK) coupled to an Agilent 6890N GC equipped with a DB-5 ms 30 m column (0.25 mm internal diameter, 0.25 mM film thickness, Agilent, USA). The inlet temperature was maintained at 250°C and samples were injected in split 10 mode. He carrier gas flow was adjusted to 1 ml min-1. Alkanes were used for referencing of retention times.
For further details (GC-MS sample preparation and analysis) see the related publication (Rickert et al. 2016 PLOS ONE).
Rickert, Esther; Karsten, Ulf; Pohnert, Georg; Wahl, Martin (2016): Investigations on Fucus vesiculosus and Fucus serratus at outer Kiel Fjord over one year (August 2012 - July 2013). PANGAEA, https://doi.org/10.1594/PANGAEA.858055
Latitude: 54.455830 * Longitude: 10.199160
Date/Time Start: 2012-08-01T00:00:00 * Date/Time End: 2013-07-31T00:00:00
Datasets listed in this publication series
- Rickert, E; Wahl, M; Link, H et al. (2016): Seasonal variations in surface metabolite composition of Fucus serratus sampled at outer Kiel Fjord (August 2012 - July 2013). https://doi.org/10.1594/PANGAEA.864959
- Rickert, E; Wahl, M; Link, H et al. (2016): Seasonal variations in surface metabolite composition of Fucus vesiculosus sampled at outer Kiel Fjord (August 2012 - July 2013). https://doi.org/10.1594/PANGAEA.864958