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Nasrolahi, Ali; Havenhand, Jon N; Wrange, Anna-Lisa; Pansch, Christian (2016): Experiment II: Effect of temperature and salinity on survival of cyprids (Fig. 3b). GEOMAR - Helmholtz Centre for Ocean Research Kiel, PANGAEA,, In supplement to: Nasrolahi, A et al. (2016): Population and life-stage specific sensitivities to temperature and salinity stress in barnacles. Scientific Reports, 6, 32263,

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Experiment II - cypris settlement
Newly released nauplius larvae were held at a density of 0.5 larvae ml-1 in filtered (0.2 µm) seawater in 20 l containers at 25°C and a salinity of 30 ("standard" culture conditions at the time of collection, (see Berntsson et al. for culture details 31)). Containers were gently aerated and provided with a 1:1 mixture of the unicellular algae, T. pseudonana and S. marinoi, at a concentration of 2 × 105 cells ml-1. Every third day, the water in each container was carefully replaced by filtered seawater and fresh food was added. When cyprids appeared in the cultures (usually after 6 to 7 days), the cultures were sieved (200 µm) and cyprids were collected. Ten cyprids were incubated under continuous light in 10 ml filtered (0.2 µm) seawater in each well of the six-well plates at the temperature and salinity combinations outlined above. The transition from initial to target temperature and salinity conditions was achieved gradually (2 salinity units h-1, 1°C h-1) to prevent acute shock to the cyprids. Every second day, the water in each well was carefully replaced with fresh filtered seawater at the respective temperature and salinity. Settlement and survival of cyprids were monitored daily over 26 days. From these data we calculated: i) survival and ii) settlement of cyprids.
#NameShort NameUnitPrincipal InvestigatorMethodComment
1ReplicateReplicatePansch, Christian
2Day of experimentDOEdayPansch, Christian
3Treatment: temperatureTreat temp°CPansch, Christian
4TreatmentTreatPansch, ChristianSalinity
5SettlementSettlement%Pansch, Christiancyprid settlement
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