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Nasrolahi, Ali; Havenhand, Jonathan N; Wrange, Anna-Lisa; Pansch, Christian (2016): Experiment I: Effect of temperature and salinity on naupliar duration (Fig. 1b). GEOMAR - Helmholtz Centre for Ocean Research Kiel, PANGAEA,, In supplement to: Nasrolahi, A et al. (2016): Population and life-stage specific sensitivities to temperature and salinity stress in barnacles. Scientific Reports, 6, 32263,

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Experiment I - nauplius to cypris development
Twenty nauplii were incubated in 10 ml filtered (0.2 µm) seawater in each well of the six-well plates under continuous light, and fed daily with a 1:1 mixture of the unicellular algae, C. calcitrans and S. marinoi, at a concentration of 2 × 105 cells ml-1 21. Every second day, the water in each well was carefully replaced by filtered seawater at the respective temperature and salinity, and fresh food was added. The number of surviving nauplii, cyprids and settled juveniles in each experimental well were observed daily for 21 days using a dissecting microscope (Olympus SZX12). Preliminary trials confirmed that the short handling time of each six-well plate during observations did not cause any significant change in water temperature in any of the treatments.
The resulting data were used to calculate: i) survival (% survivors in form of nauplii, cyprids or settled individuals at the end of the experiment relative to the initial number of nauplii), ii) naupliar duration (days from hatching until 50 % of the surviving nauplii had metamorphosed into cyprids), iii) successful metamorphosis to cyprids (% of the initial number of nauplii that metamorphosed into cyprids at the end of the experiment), and iv) overall settlement success (number of settled cyprids at the end of the experiment relative to the initial number of nauplii). We additionally calculated v) settlement of cyprids (number of successfully settled cyprids at the end of the experiment relative to metric iii).
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Treatment: temperatureTreat temp°CPansch, Christian
2TreatmentTreatPansch, ChristianSalinity
3Duration, number of daysDurationdaysPansch, Christianlarval duration
159 data points

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