Citation:

fbv057

Name: Copepod vital rates under CO2-induced acidification: a calanoid species and a cyclopoid species under short-term exposures
Published: 2015
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Acartia grani; Animalia; Arthropoda; Behaviour; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Laboratory experiment; Laboratory strains; Mediterranean Sea; Oithona davisae; Pelagos; Reproduction; Respiration; Single species; Zooplankton

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Abstract:

Although copepods have been considered tolerant against the direct influence of the ocean acidification (OA) projected for the end of the century, some recent studies have challenged this view. Here, we have examined the direct impact of short-term exposure to a pCO2/pH level relevant for the year 2100 (pHNBS, control: 8.18, low pH: 7.78), on the physiological performance of two representative marine copepods: the calanoid Acartia grani and the cyclopoid Oithona davisae. Adults of both species, from laboratory cultures, were preconditioned for four consecutive days in algal suspensions (Akashiwo sanguinea) prepared with filtered sea water pre-adjusted to the targeted pH values via CO2 bubbling. We measured the feeding and respiratory activity and reproductive output of those pre-conditioned females. The largely unaffected fatty acid composition of the prey offered between OA treatments and controls supports the absence in the study of indirect OA effects (i.e. changes of food nutritional quality). Our results show no direct effect of acidification on the vital rates examined in either copepod species. Our findings are compared with results from previous short- and long-term manipulative experiments on other copepod species.

Keyword(s):

Acartia grani; Animalia; Arthropoda; Behaviour; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Laboratory experiment; Laboratory strains; Mediterranean Sea; Oithona davisae; Pelagos; Reproduction; Respiration; Single species; Zooplankton

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.8. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2016-05-24.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Isari, Stamatina		study
2	Species	Species		Isari, Stamatina
3	Registration number of species	Reg spec no		Isari, Stamatina
4	Uniform resource locator/link to reference	URL ref		Isari, Stamatina		WoRMS Aphia ID
5	Treatment	Treat		Isari, Stamatina
6	Clearance rate	CR	ml/#/day	Isari, Stamatina
7	Ingestion rate per individual	IR	#/#/day	Isari, Stamatina
8	Egg production rate per female	EPR	#/female/day	Isari, Stamatina
9	Eggs per clutch	Eggs/clutch	#	Isari, Stamatina
10	Respiration rate, oxygen, per individual	Resp O2/ind	nmol/#/day	Isari, Stamatina
11	Time in hours	Time	h	Isari, Stamatina
12	Group	Group		Isari, Stamatina		FA content
13	Fatty acid content	FA con	pg/#	Isari, Stamatina
14	Fatty acid content, standard deviation	FA con std dev	±	Isari, Stamatina
15	Fatty acid composition	FA comp	%	Isari, Stamatina
16	Fatty acids, standard deviation	FA std dev	±	Isari, Stamatina
17	Temperature, water	Temp	°C	Isari, Stamatina
18	Salinity	Sal		Isari, Stamatina
19	pH	pH		Isari, Stamatina	Potentiometric	NBS scale
20	pH, standard deviation	pH std dev	±	Isari, Stamatina	Potentiometric	NBS scale
21	pH	pH		Isari, Stamatina	Calculated using CO2SYS	total scale
22	pH, standard deviation	pH std dev	±	Isari, Stamatina	Calculated using CO2SYS	total scale
23	Alkalinity, total	AT	µmol/kg	Isari, Stamatina	Potentiometric titration
24	Alkalinity, total, standard deviation	AT std dev	±	Isari, Stamatina	Potentiometric titration
25	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Isari, Stamatina	Calculated using CO2SYS
26	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Isari, Stamatina	Calculated using CO2SYS
27	Dry air column-averaged mixing ratio of carbon dioxide	XCO2	ppmv	Isari, Stamatina	Calculated using CO2SYS
28	Dry air column-averaged mixing ratio of carbon dioxide, standard deviation	XCO2 std dev	±	Isari, Stamatina	Calculated using CO2SYS
29	Carbon, inorganic, dissolved	DIC	µmol/kg	Isari, Stamatina	Calculated using CO2SYS
30	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Isari, Stamatina	Calculated using CO2SYS
31	Bicarbonate ion	[HCO3]-	µmol/kg	Isari, Stamatina	Calculated using CO2SYS
32	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Isari, Stamatina	Calculated using CO2SYS
33	Carbonate ion	[CO3]2-	µmol/kg	Isari, Stamatina	Calculated using CO2SYS
34	Carbonate ion, standard deviation	[CO3]2- std dev	±	Isari, Stamatina	Calculated using CO2SYS
35	Aragonite saturation state	Omega Arg		Isari, Stamatina	Calculated using CO2SYS
36	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Isari, Stamatina	Calculated using CO2SYS
37	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
38	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
39	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
40	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
41	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
43	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
44	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
45	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
46	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

2908 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Treat	6 CR [ml/#/day]	7 IR [#/#/day]	8 EPR [#/female/day]	9 Eggs/clutch [#]	10 Resp O2/ind [nmol/#/day]	11 Time [h]	12 Group (FA content)	13 FA con [pg/#]	14 FA con std dev [±]	15 FA comp [%]	16 FA std dev [±]	17 Temp [°C]	18 Sal	19 pH (NBS scale, Potentiometric)	20 pH std dev [±] (NBS scale, Potentiometric)	21 pH (total scale, Calculated using...)	22 pH std dev [±] (total scale, Calculated using...)	23 AT [µmol/kg] (Potentiometric titration)	24 AT std dev [±] (Potentiometric titration)	25 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	26 pCO2 std dev [±] (Calculated using CO2SYS)	27 XCO2 [ppmv] (Calculated using CO2SYS)	28 XCO2 std dev [±] (Calculated using CO2SYS)	29 DIC [µmol/kg] (Calculated using CO2SYS)	30 DIC std dev [±] (Calculated using CO2SYS)	31 [HCO3]- [µmol/kg] (Calculated using CO2SYS)	32 [HCO3]- std dev [±] (Calculated using CO2SYS)	33 [CO3]2- [µmol/kg] (Calculated using CO2SYS)	34 [CO3]2- std dev [±] (Calculated using CO2SYS)	35 Omega Arg (Calculated using CO2SYS)	36 Omega Arg std dev [±] (Calculated using CO2SYS)	37 CSC flag (Calculated using seacarb afte...)	38 pH (total scale, Calculated using...)	39 CO2 [µmol/kg] (Calculated using seacarb afte...)	40 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	41 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	42 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	43 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	44 DIC [µmol/kg] (Calculated using seacarb afte...)	45 Omega Arg (Calculated using seacarb afte...)	46 Omega Cal (Calculated using seacarb afte...)
laboratory	Acartia grani (zooplankton)	232027	marinespecies.org	control	34.40	1248.00	69.00									18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Acartia grani (zooplankton)	232027	marinespecies.org	control	34.40	1245.00	48.00									18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Acartia grani (zooplankton)	232027	marinespecies.org	control	32.40	1187.00	60.00									18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Acartia grani (zooplankton)	232027	marinespecies.org	control	34.40	1170.00	45.00									18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Acartia grani (zooplankton)	232027	marinespecies.org	low pH	47.30	1673.00	55.00									18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Acartia grani (zooplankton)	232027	marinespecies.org	low pH	30.70	1157.00	55.00									18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Acartia grani (zooplankton)	232027	marinespecies.org	low pH	37.70	1377.00	57.00									18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Acartia grani (zooplankton)	232027	marinespecies.org	low pH	39.70	1365.00	66.00									18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Oithona davisae (zooplankton)	353995	marinespecies.org	control	0.34	14.58	1.33	6.4								18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Oithona davisae (zooplankton)	353995	marinespecies.org	control	0.21	9.26	1.72	7.5								18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Oithona davisae (zooplankton)	353995	marinespecies.org	control	0.26	11.41	2.31	8.2								18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Oithona davisae (zooplankton)	353995	marinespecies.org	control	0.47	19.76	1.88	7.7								18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Oithona davisae (zooplankton)	353995	marinespecies.org	low pH	0.34	15.12	1.88	7.2								18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Oithona davisae (zooplankton)	353995	marinespecies.org	low pH	0.34	15.29	2.01	7.9								18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Oithona davisae (zooplankton)	353995	marinespecies.org	low pH	0.22	10.16	2.10	8.3								18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Oithona davisae (zooplankton)	353995	marinespecies.org	low pH	0.24	11.21	1.51	7.2								18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Acartia grani (zooplankton)			control					29.36							18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Acartia grani (zooplankton)			control					40.95							18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Acartia grani (zooplankton)			low pH					61.42							18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Acartia grani (zooplankton)			low pH					55.70							18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Oithona davisae (zooplankton)			control					3.53							18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Oithona davisae (zooplankton)			control					5.63							18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Oithona davisae (zooplankton)			low pH					4.57							18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Oithona davisae (zooplankton)			low pH					6.28							18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Acartia grani (zooplankton)			control						0	14:00	75.2	3.8	7.0	0.5	18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Acartia grani (zooplankton)			control						0	16:00	371.9	13.0	34.4	0.3	18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Acartia grani (zooplankton)			control						0	18:00	26.2	7.2	2.4	0.6	18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Acartia grani (zooplankton)			control						0	16:1(n-7)	42.0	0.5	3.9	0.1	18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Acartia grani (zooplankton)			control						0	18:1(n-7)	25.2	0.8	2.3	0.0	18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Acartia grani (zooplankton)			control						0	18:1(n-9)	113.9	11.4	10.5	0.6	18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Acartia grani (zooplankton)			control						0	18:4(n-3)	29.2	0.5	2.7	0.1	18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Acartia grani (zooplankton)			control						0	18:5(n-3)	41.4	2.3	3.8	0.3	18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Acartia grani (zooplankton)			control						0	20:5(n-3)	76.3	2.8	7.1	0.3	18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Acartia grani (zooplankton)			control						0	22:6(n-3)	229.4	4.8	21.2	0.5	18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Acartia grani (zooplankton)			control						0	total monounsaturated FA	199.6	15.1	18.5	0.7	18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Acartia grani (zooplankton)			control						0	total polyunsaturated FA	393.5	10.9	36.4	0.6	18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Acartia grani (zooplankton)			control						0	Total	1080.4	46.4			18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Acartia grani (zooplankton)			low pH						0	14:00	77.7	9.0	6.8	0.6	18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Acartia grani (zooplankton)			low pH						0	16:00	399.9	13.0	35.1	0.6	18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Acartia grani (zooplankton)			low pH						0	18:00	23.1	1.1	2.0	0.0	18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Acartia grani (zooplankton)			low pH						0	16:1(n-7)	43.9	2.2	3.9	0.1	18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Acartia grani (zooplankton)			low pH						0	18:1(n-7)	23.6	0.7	2.1	0.1	18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Acartia grani (zooplankton)			low pH						0	18:1(n-9)	117.3	4.5	10.3	0.3	18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Acartia grani (zooplankton)			low pH						0	18:4(n-3)	31.8	0.8	2.8	0.0	18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Acartia grani (zooplankton)			low pH						0	18:5(n-3)	43.4	2.6	3.8	0.2	18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Acartia grani (zooplankton)			low pH						0	20:5(n-3)	80.1	3.0	7.0	0.2	18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Acartia grani (zooplankton)			low pH						0	22:6(n-3)	245.7	4.2	21.6	0.3	18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Acartia grani (zooplankton)			low pH						0	total monounsaturated FA	204.1	8.0	17.9	0.6	18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Acartia grani (zooplankton)			low pH						0	total polyunsaturated FA	416.1	10.6	36.6	0.6	18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Acartia grani (zooplankton)			low pH						0	Total	1138.3	32.3			18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Acartia grani (zooplankton)			control						24	14:00	63.8	3.4	6.2	0.2	18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Acartia grani (zooplankton)			control						24	16:00	335.4	11.2	32.5	0.1	18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Acartia grani (zooplankton)			control						24	18:00	20.5	0.8	2.0	0.0	18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Acartia grani (zooplankton)			control						24	16:1(n-7)	44.9	1.1	4.3	0.1	18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Acartia grani (zooplankton)			control						24	18:1(n-7)	37.1	1.1	3.6	0.2	18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Acartia grani (zooplankton)			control						24	18:1(n-9)	87.4	2.8	8.5	0.0	18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Acartia grani (zooplankton)			control						24	18:4(n-3)	28.1	1.0	2.7	0.0	18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Acartia grani (zooplankton)			control						24	18:5(n-3)	47.6	1.8	4.6	0.1	18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Acartia grani (zooplankton)			control						24	20:5(n-3)	81.3	3.4	7.9	0.1	18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Acartia grani (zooplankton)			control						24	22:6(n-3)	229.4	12.4	22.2	0.5	18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Acartia grani (zooplankton)			control						24	total monounsaturated FA	195.5	2.4	18.9	0.5	18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Acartia grani (zooplankton)			control						24	total polyunsaturated FA	398.8	18.7	38.6	0.6	18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Acartia grani (zooplankton)			control						24	Total	1032.5	36.0			18.5	37.4	8.18	0.01	8.06	0.01	2517	6	419	9	428	10	2229	10	2009	12	206	2	3.13	0.03	26	8.06	13.74	411.98	413.41	2008.29	207.71	2229.74	3.16	4.87
laboratory	Acartia grani (zooplankton)			low pH						24	14:00	63.6	14.0	6.0	0.8	18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Acartia grani (zooplankton)			low pH						24	16:00	369.4	14.7	35.5	3.8	18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Acartia grani (zooplankton)			low pH						24	18:00	22.6	1.1	2.2	0.2	18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Acartia grani (zooplankton)			low pH						24	16:1(n-7)	45.3	3.8	4.3	0.1	18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Acartia grani (zooplankton)			low pH						24	18:1(n-7)	29.4	1.4	2.8	0.2	18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Acartia grani (zooplankton)			low pH						24	18:1(n-9)	91.1	6.0	8.7	0.5	18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Acartia grani (zooplankton)			low pH						24	18:4(n-3)	28.5	4.9	2.7	0.2	18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Acartia grani (zooplankton)			low pH						24	18:5(n-3)	51.2	8.7	4.9	0.4	18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Acartia grani (zooplankton)			low pH						24	20:5(n-3)	81.7	14.9	7.8	0.7	18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Acartia grani (zooplankton)			low pH						24	22:6(n-3)	215.0	44.1	20.4	2.5	18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Acartia grani (zooplankton)			low pH						24	total monounsaturated FA	185.0	15.7	17.7	0.6	18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Acartia grani (zooplankton)			low pH						24	total polyunsaturated FA	388.8	73.6	36.9	3.8	18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22
laboratory	Acartia grani (zooplankton)			low pH						24	Total	1047.1	104.1			18.5	37.4	7.78	0.01	7.66	0.01	2526	9	1203	17	1229	17	2429	6	2296	5	94	2	1.43	0.03	26	7.66	39.47	1183.02	1187.13	2295.07	94.47	2429.00	1.44	2.22