Bach, Lennart Thomas; Riebesell, Ulf; Gutowska, Magdalena A; Federwisch, Luisa; Schulz, Kai Georg (2015): Substrate-inhibitor test with E. huxleyi [dataset]. GEOMAR - Helmholtz Centre for Ocean Research Kiel, PANGAEA, https://doi.org/10.1594/PANGAEA.860443, In supplement to: Bach, LT et al. (2015): A unifying concept of coccolithophore sensitivity to changing carbonate chemistry embedded in an ecological framework. Progress in Oceanography, 135, 125-138, https://doi.org/10.1016/j.pocean.2015.04.012
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Project(s):
Comment:
Carbonate chemistry and calcification data of the substrate-inhibitor interaction experiment with E. huxleyi. The percentage of 200 cells possessing/producing coccoliths was investigated by means of cross-polarized light microscopy which allows detection of internal coccoliths. These birefringence measurements were backed up with scanning electron microscopy (SEM) investigations when the presence of coccoliths could not be unequivocally determined. In case of discrepancies among the two methods, we relied on SEM investigations since this is the most reliable approach.
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Experimental treatment | Exp treat | Bach, Lennart Thomas | |||
| 2 | Temperature, water | Temp | °C | Bach, Lennart Thomas | ||
| 3 | Radiation, photosynthetically active | PAR | µmol quanta/m2/s | Bach, Lennart Thomas | ||
| 4 | Light:Dark cycle | L:D | hh:hh | Bach, Lennart Thomas | ||
| 5 | Alkalinity, total | AT | µmol/kg | Bach, Lennart Thomas | ||
| 6 | Carbon, inorganic, dissolved | DIC | µmol/kg | Bach, Lennart Thomas | ||
| 7 | pH | pH | Bach, Lennart Thomas | free scale | ||
| 8 | Hydrogen ion concentration | H+ | µmol/kg | Bach, Lennart Thomas | free scale | |
| 9 | Fugacity of carbon dioxide (water) at sea surface temperature (wet air) | fCO2water_SST_wet | µatm | Bach, Lennart Thomas | ||
| 10 | Bicarbonate ion | [HCO3]- | µmol/kg | Bach, Lennart Thomas | ||
| 11 | Carbonate ion | [CO3]2- | µmol/kg | Bach, Lennart Thomas | ||
| 12 | Carbon dioxide | CO2 | µmol/kg | Bach, Lennart Thomas | ||
| 13 | Calcite saturation state | Omega Cal | Bach, Lennart Thomas | |||
| 14 | Coccoliths | Cocco | % | Bach, Lennart Thomas | Percentage of 200 cells possessing coccoliths | |
| 15 | Coccoliths | Cocco | Bach, Lennart Thomas | Presence of coccoliths confirmed by means of SEM |
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Size:
771 data points
Data
| 1 Exp treat | 2 Temp [°C] | 3 PAR [µmol quanta/m2/s] | 4 L:D [hh:hh] | 5 AT [µmol/kg] | 6 DIC [µmol/kg] | 7 pH | 8 H+ [µmol/kg] | 9 fCO2water_SST_wet [µatm] | 10 [HCO3]- [µmol/kg] | 11 [CO3]2- [µmol/kg] | 12 CO2 [µmol/kg] | 13 Omega Cal | 14 Cocco [%] | 15 Cocco |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 469.5 | 488.7 | 7.13 | 0.0749 | 957 | 449 | 4 | 35.8 | 0.10 | 0.4 | no |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 485.6 | 494.5 | 7.23 | 0.0593 | 777 | 460 | 5 | 29.1 | 0.12 | 0.0 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 497.6 | 494.9 | 7.34 | 0.0455 | 602 | 465 | 7 | 22.6 | 0.16 | 0.5 | no |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 509.4 | 490.4 | 7.50 | 0.0315 | 417 | 465 | 10 | 15.6 | 0.23 | 0.0 | no |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 522.9 | 490.4 | 7.62 | 0.0238 | 315 | 466 | 13 | 11.8 | 0.31 | 0.0 | no |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 636.0 | 668.6 | 7.11 | 0.0771 | 1344 | 613 | 5 | 50.3 | 0.13 | 0.0 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 678.1 | 679.4 | 7.36 | 0.0440 | 801 | 640 | 10 | 30.0 | 0.23 | 0.5 | no |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 690.9 | 686.6 | 7.40 | 0.0395 | 728 | 648 | 11 | 27.3 | 0.26 | 2.4 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 707.1 | 685.4 | 7.54 | 0.0289 | 534 | 650 | 15 | 20.0 | 0.36 | 9.3 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 723.1 | 688.6 | 7.63 | 0.0232 | 432 | 654 | 19 | 16.2 | 0.45 | 32.9 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 572.9 | 585.4 | 7.23 | 0.0584 | 905 | 545 | 6 | 33.9 | 0.15 | 0.0 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 580.2 | 592.8 | 7.24 | 0.0581 | 913 | 552 | 6 | 34.2 | 0.15 | 0.0 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 583.1 | 589.5 | 7.29 | 0.0515 | 809 | 552 | 7 | 30.3 | 0.17 | 0.0 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 590.9 | 589.2 | 7.36 | 0.0437 | 689 | 555 | 8 | 25.8 | 0.20 | 0.0 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 594.8 | 589.8 | 7.39 | 0.0408 | 646 | 556 | 9 | 24.2 | 0.22 | 0.0 | yes |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 877.8 | 538.8 | 8.71 | 0.0020 | 22 | 401 | 137 | 0.8 | 3.26 | 97.6 | yes |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 463.6 | 491.7 | 7.05 | 0.0895 | 1135 | 446 | 3 | 42.5 | 0.08 | 0.0 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 501.1 | 498.4 | 7.34 | 0.0455 | 606 | 469 | 7 | 22.7 | 0.16 | 0.0 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 539.5 | 501.3 | 7.67 | 0.0213 | 289 | 476 | 15 | 10.8 | 0.36 | 0.0 | no |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 600.2 | 509.8 | 8.00 | 0.0100 | 135 | 473 | 32 | 5.0 | 0.75 | 12.6 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 658.1 | 506.2 | 8.25 | 0.0056 | 72 | 450 | 54 | 2.7 | 1.28 | 9.6 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 586.1 | 595.3 | 7.27 | 0.0543 | 859 | 556 | 7 | 32.2 | 0.16 | 0.0 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 597.4 | 598.9 | 7.33 | 0.0464 | 743 | 563 | 8 | 27.8 | 0.19 | 0.5 | no |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 612.6 | 604.1 | 7.42 | 0.0378 | 614 | 571 | 10 | 23.0 | 0.24 | 7.8 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 628.4 | 604.0 | 7.56 | 0.0277 | 452 | 573 | 14 | 16.9 | 0.33 | 31.8 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 640.5 | 606.1 | 7.64 | 0.0231 | 378 | 575 | 17 | 14.2 | 0.40 | 30.7 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 724.9 | 462.5 | 8.58 | 0.0026 | 28 | 368 | 93 | 1.0 | 2.22 | 93.7 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 521.8 | 488.3 | 7.63 | 0.0233 | 307 | 464 | 13 | 11.5 | 0.32 | 31.9 | yes |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 892.1 | 951.7 | 7.07 | 0.0846 | 2085 | 867 | 7 | 78.1 | 0.16 | 11.0 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 913.5 | 951.2 | 7.19 | 0.0648 | 1625 | 881 | 9 | 60.9 | 0.22 | 21.2 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 926.2 | 959.4 | 7.22 | 0.0608 | 1542 | 892 | 10 | 57.8 | 0.23 | 39.6 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 939.8 | 949.5 | 7.35 | 0.0442 | 1124 | 894 | 14 | 42.1 | 0.32 | 61.4 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 954.1 | 946.0 | 7.47 | 0.0342 | 871 | 896 | 18 | 32.6 | 0.42 | 79.7 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 317.0 | 326.8 | 7.11 | 0.0774 | 659 | 300 | 3 | 24.7 | 0.06 | 0.0 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 737.9 | 380.2 | 8.84 | 0.0014 | 11 | 260 | 120 | 0.4 | 2.86 | 0.0 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 650.5 | 442.7 | 8.45 | 0.0036 | 38 | 372 | 70 | 1.4 | 1.66 | 0.0 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 977.8 | 550.2 | 8.87 | 0.0014 | 14 | 368 | 182 | 0.5 | 4.34 | 0.0 | no |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 1663.5 | 1028.5 | 8.93 | 0.0012 | 22 | 655 | 373 | 0.8 | 8.89 | 95.4 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 421.0 | 428.7 | 7.21 | 0.0623 | 705 | 398 | 4 | 26.4 | 0.10 | 0.0 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 519.2 | 538.2 | 7.16 | 0.0700 | 988 | 496 | 5 | 37.0 | 0.11 | 0.0 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 543.9 | 537.5 | 7.39 | 0.0408 | 588 | 507 | 8 | 22.0 | 0.20 | 0.0 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 590.2 | 582.6 | 7.41 | 0.0389 | 608 | 550 | 9 | 22.8 | 0.23 | 0.0 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 737.2 | 691.4 | 7.71 | 0.0194 | 362 | 655 | 23 | 13.6 | 0.54 | 48.5 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 450.8 | 471.9 | 7.10 | 0.0799 | 981 | 432 | 4 | 36.7 | 0.09 | 0.0 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 485.8 | 480.5 | 7.36 | 0.0433 | 558 | 453 | 7 | 20.9 | 0.17 | 0.0 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 531.9 | 482.2 | 7.76 | 0.0174 | 225 | 456 | 18 | 8.4 | 0.42 | 0.0 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 628.6 | 489.3 | 8.21 | 0.0061 | 76 | 439 | 48 | 2.9 | 1.14 | 1.0 | yes |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 772.0 | 485.9 | 8.62 | 0.0024 | 26 | 379 | 106 | 1.0 | 2.53 | 1.0 | yes |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 526.3 | 548.3 | 7.13 | 0.0734 | 1053 | 504 | 5 | 39.5 | 0.11 | 0.0 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 558.3 | 562.9 | 7.29 | 0.0507 | 761 | 527 | 7 | 28.5 | 0.17 | 0.0 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 715.1 | 776.6 | 6.99 | 0.1026 | 2031 | 696 | 5 | 76.1 | 0.11 | 0.0 | |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 746.4 | 790.8 | 7.09 | 0.0809 | 1662 | 723 | 6 | 62.3 | 0.14 | 0.5 | no |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 785.9 | 800.4 | 7.29 | 0.0513 | 1094 | 750 | 10 | 41.0 | 0.23 | 2.0 | yes |
| substate-inhibitor interaction test | 15 | 150 | 16:08 | 208.9 | 233.1 | 6.81 | 0.1541 | 874 | 200 | 1 | 32.8 | 0.02 | 0.0 |