Citation:

1901.pdf

Name: Response of a natural phytoplankton community from the Qingdao coast (Yellow Sea, China) to variable CO2 levels over a short-term incubation experiment
Published: 2015
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Community composition and diversity; Entire community; Laboratory experiment; North Pacific; Pelagos; Primary production/Photosynthesis; Temperate

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Abstract:

Since marine phytoplankton play a vital role in stabilizing earth's climate by removing significant amount of atmospheric CO2, their responses to increasing CO2 levels are indeed vital to address. The responses of a natural phytoplankton community from the Qingdao coast (NW Yellow Sea, China) was studied under different CO2 levels in microcosms. HPLC pigment analysis revealed the presence of diatoms as a dominant microalgal group; however, members of chlorophytes, prasinophytes, cryptophytes and cyanophytes were also present. delta 13CPOM values indicated that the phytoplankton community probably utilized bicarbonate ions as dissolved inorganic carbon source through a carbon concentration mechanism (CCM) under low CO2 levels, and diffusive CO2 uptake increased upon the increase of external CO2 levels. Although, considerable increase in phytoplankton biomass was noticed in all CO2 treatments, CO2-induced effects were absent. Higher net nitrogen uptake under low CO2 levels could be related to the synthesis of CCM components. Flow cytometry analysis showed slight reduction in the abundance of Synechococcus and pico-eukaryotes under the high CO2 treatments. Diatoms did not show any negative impact in response to increasing CO2 levels; however, chlorophytes revealed a reverse tend. Heterotrophic bacterial count enhanced with increasing CO2 levels and indicated higher abundance of labile organic carbon. Thus, the present study indicates that any change in dissolved CO2 concentrations in this area may affect phytoplankton physiology and community structure and needs further long-term study.

Keyword(s):

Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Community composition and diversity; Entire community; Laboratory experiment; North Pacific; Pelagos; Primary production/Photosynthesis; Temperate

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.8. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

The value of biological parameters under 3 pCO2 levels are the final day value. In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2016-05-13.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Biswas, Haimanti		study
2	Treatment	Treat		Biswas, Haimanti
3	Chlorophyll a	Chl a	µg/l	Biswas, Haimanti
4	Chlorophyll a, standard deviation	Chl a std dev	±	Biswas, Haimanti
5	Carbon, organic, particulate	POC	µmol/l	Biswas, Haimanti
6	Carbon, organic, particulate, standard deviation	POC std dev	±	Biswas, Haimanti
7	Chlorophyll a/particulate organic carbon ratio	Chl a/POC		Biswas, Haimanti
8	Chlorophyll a/particulate organic carbon ratio, standard deviation	Chl a/POC std dev	±	Biswas, Haimanti
9	Nitrogen, organic, particulate	PON	µmol/l	Biswas, Haimanti
10	Nitrogen, organic, particulate, standard deviation	PON std dev	±	Biswas, Haimanti
11	Phosphorus, organic, particulate	POP	µmol/l	Biswas, Haimanti
12	Particulate organic phosphorus, standard deviation	POP std dev	±	Biswas, Haimanti
13	Carbon/Nitrogen ratio	C/N		Biswas, Haimanti
14	Carbon/Nitrogen ratio, standard deviation	C/N std dev	±	Biswas, Haimanti
15	Carbon/Phosphorus ratio	C/P		Biswas, Haimanti
16	Carbon/Phosphorus ratio, standard deviation	C/P std dev	±	Biswas, Haimanti
17	Nitrogen/Phosphorus ratio	N/P		Biswas, Haimanti
18	Nitrogen/Phosphorus ratio, standard deviation	N/P std dev	±	Biswas, Haimanti
19	Nitrogen, inorganic, dissolved	DIN	µmol/l	Biswas, Haimanti
20	Nitrogen, inorganic, dissolved, standard deviation	DIN std dev	±	Biswas, Haimanti
21	Silicate	Si(OH)4	µmol/l	Biswas, Haimanti
22	Silicate, standard deviation	Si(OH)4 std dev	±	Biswas, Haimanti
23	Phosphorus, inorganic, dissolved	DIP	µmol/l	Biswas, Haimanti
24	Phosphorus, inorganic, dissolved, standard deviation	DIP std dev	±	Biswas, Haimanti
25	Nitrogen, inorganic, dissolved/Phosphorus, inorganic, dissolved ratio	DIN/DIP		Biswas, Haimanti
26	Nitrogen, inorganic, dissolved/Phosphorus, inorganic, dissolved ratio, standard deviation	DIN/DIP std dev	±	Biswas, Haimanti
27	Nitrogen/Silicon ratio	N/Si		Biswas, Haimanti
28	Nitrogen/Silicon ratio, standard deviation	N/Si std dev	±	Biswas, Haimanti
29	Carbon, organic, dissolved	DOC	µmol/l	Biswas, Haimanti
30	Carbon, organic, dissolved, standard deviation	DOC std dev	±	Biswas, Haimanti
31	Dissolved inorganic nitrogen, uptake	DIN upt	µmol/l	Biswas, Haimanti
32	Dissolved inorganic nitrogen, uptake, standard deviation	DIN upt std dev	±	Biswas, Haimanti
33	Silicate uptake	Si(OH)4 upt	µmol/l	Biswas, Haimanti
34	Silicon uptake, standard deviation	Si upt std dev	±	Biswas, Haimanti
35	Phosphorus uptake	P upt	µmol/l	Biswas, Haimanti
36	Phosphorus uptake, standard deviation	P upt std dev	±	Biswas, Haimanti
37	Silicon/Nitrogen uptake ratio	Si/N upt		Biswas, Haimanti
38	Silicon/Nitrogen uptake ratio, standard deviation	Si/N upt std dev	±	Biswas, Haimanti
39	Nitrogen/Phosphorus uptake ratio	N/P upt		Biswas, Haimanti
40	Nitrogen/Phosphorus uptake ratio, standard deviation	N/P upt std dev	±	Biswas, Haimanti
41	Silicon/phosphorus uptake ratio	Si/P upt		Biswas, Haimanti
42	Silicon/phosphorus uptake ratio, standard deviation	Si/P upt std dev	±	Biswas, Haimanti
43	Consumption of carbon, inorganic, dissolved	DIC consumpt	µmol/l	Biswas, Haimanti		net
44	Consumption of carbon, inorganic, dissolved, standard deviation	DIC consumpt std dev	±	Biswas, Haimanti		net
45	Carbon, organic, dissolved + particulate, net production	NP DOC+POC	µmol/l	Biswas, Haimanti
46	Carbon, organic, dissolved + particulate, net production, standard deviation	NP DOC+POC std dev	±	Biswas, Haimanti
47	δ13C	δ13C	‰ PDB	Biswas, Haimanti
48	δ13C, standard deviation	δ13C std dev	±	Biswas, Haimanti
49	δ15N	δ15N	‰ air	Biswas, Haimanti
50	δ15N, standard deviation	δ15N std dev	±	Biswas, Haimanti
51	Fucoxanthin/chlorophyll a ratio	Fuco/Chl a		Biswas, Haimanti
52	Fucoxanthin/chlorophyll a ratio, standard devitation	Fuco/Chl a std dev	±	Biswas, Haimanti
53	Diatoxanthin index	DT index		Biswas, Haimanti
54	Diatoxanthin index, standard deviation	DT index std dev	±	Biswas, Haimanti
55	Chlorophyll a/Chlorophyll b ratio	Chl a/Chl b		Biswas, Haimanti
56	Violaxanthin/antheraxanthin ratio	Viola/Anthx		Biswas, Haimanti
57	Violaxanthin/antheraxanthin ratio, standard deviation	Viola/Anthx std dev	±	Biswas, Haimanti
58	Lutein/chlorophyll a ratio	Lut/Chl a		Biswas, Haimanti
59	Lutein/chlorophyll a ratio, standard deviation	Lut/Chl a std dev	±	Biswas, Haimanti
60	Violaxanthin/Zeaxanthin ratio	Viola/Zea		Biswas, Haimanti
61	Violaxanthin/Zeaxanthin ratio, standard deviation	Viola/Zea std dev	±	Biswas, Haimanti
62	Neoxanthin/chlorophyll a ratio	Neo/chl a		Biswas, Haimanti
63	Neoxanthin/chlorophyll a ratio, standard deviation	Neo/Chl a std dev	±	Biswas, Haimanti
64	Violaxanthin/chlorophyll a ratio	Viola/chl a		Biswas, Haimanti
65	Violaxanthin/chlorophyll a ratio, standard deviation	Viola/Chl a std dev	±	Biswas, Haimanti
66	Bacteria, heterotrophic	HBA	#/ml	Biswas, Haimanti
67	Bacteria, heterotrophic, standard deviation	Bact het std dev	±	Biswas, Haimanti
68	Picoeukaryotes	PEuk	#/ml	Biswas, Haimanti
69	Picoeukaryotes, standard deviation	PEuk std dev	±	Biswas, Haimanti
70	Synechococcus	Synechococcus	#/ml	Biswas, Haimanti
71	Synechococcus spp., standard deviation	Synechococcus spp. std dev	±	Biswas, Haimanti
72	Temperature, water	Temp	°C	Biswas, Haimanti
73	Salinity	Sal		Biswas, Haimanti
74	Alkalinity, total	AT	µmol/kg	Biswas, Haimanti		initial
75	Carbon, inorganic, dissolved	DIC	µmol/kg	Biswas, Haimanti		initial
76	Alkalinity, total	AT	µmol/kg	Biswas, Haimanti		final
77	Carbon, inorganic, dissolved	DIC	µmol/kg	Biswas, Haimanti		final
78	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
79	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial,total scale
80	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
81	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
82	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
83	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
84	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
85	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
86	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
87	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	final,total scale
88	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	final
89	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	final
90	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	final
91	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	final
92	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	final
93	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	final
94	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	final

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

679 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Treat	3 Chl a [µg/l]	4 Chl a std dev [±]	5 POC [µmol/l]	6 POC std dev [±]	7 Chl a/POC	8 Chl a/POC std dev [±]	9 PON [µmol/l]	10 PON std dev [±]	11 POP [µmol/l]	12 POP std dev [±]	13 C/N	14 C/N std dev [±]	15 C/P	16 C/P std dev [±]	17 N/P	18 N/P std dev [±]	19 DIN [µmol/l]	20 DIN std dev [±]	21 Si(OH)4 [µmol/l]	22 Si(OH)4 std dev [±]	23 DIP [µmol/l]	24 DIP std dev [±]	25 DIN/DIP	26 DIN/DIP std dev [±]	27 N/Si	28 N/Si std dev [±]	29 DOC [µmol/l]	30 DOC std dev [±]	31 DIN upt [µmol/l]	32 DIN upt std dev [±]	33 Si(OH)4 upt [µmol/l]	34 Si upt std dev [±]	35 P upt [µmol/l]	36 P upt std dev [±]	37 Si/N upt	38 Si/N upt std dev [±]	39 N/P upt	40 N/P upt std dev [±]	41 Si/P upt	42 Si/P upt std dev [±]	43 DIC consumpt [µmol/l] (net)	44 DIC consumpt std dev [±] (net)	45 NP DOC+POC [µmol/l]	46 NP DOC+POC std dev [±]	47 δ13C [‰ PDB]	48 δ13C std dev [±]	49 δ15N [‰ air]	50 δ15N std dev [±]	51 Fuco/Chl a	52 Fuco/Chl a std dev [±]	53 DT index	54 DT index std dev [±]	55 Chl a/Chl b	56 Viola/Anthx	57 Viola/Anthx std dev [±]	58 Lut/Chl a	59 Lut/Chl a std dev [±]	60 Viola/Zea	61 Viola/Zea std dev [±]	62 Neo/chl a	63 Neo/Chl a std dev [±]	64 Viola/chl a	65 Viola/Chl a std dev [±]	66 HBA [#/ml]	67 Bact het std dev [±]	68 PEuk [#/ml]	69 PEuk std dev [±]	70 Synechococcus [#/ml]	71 Synechococcus spp. std dev [±]	72 Temp [°C]	73 Sal	74 AT [µmol/kg] (initial)	75 DIC [µmol/kg] (initial)	76 AT [µmol/kg] (final)	77 DIC [µmol/kg] (final)	78 CSC flag	79 pH (initial,total scale)	80 CO2 [µmol/kg] (initial)	81 fCO2water_SST_wet [µatm] (initial)	82 pCO2water_SST_wet [µatm] (initial)	83 [HCO3]- [µmol/kg] (initial)	84 [CO3]2- [µmol/kg] (initial)	85 Omega Arg (initial)	86 Omega Cal (initial)	87 pH (final,total scale)	88 CO2 [µmol/kg] (final)	89 fCO2water_SST_wet [µatm] (final)	90 pCO2water_SST_wet [µatm] (final)	91 [HCO3]- [µmol/kg] (final)	92 [CO3]2- [µmol/kg] (final)	93 Omega Arg (final)	94 Omega Cal (final)
laboratory	Initial day	0.28	0.04	29.55	7.03	0.00081	0.00030	3.82	0.926	0.332	0.080	7.74	0.04	89.140	0.208	11.52	0.03	17.20	2.07	5.92	0.41	0.52	0.13	33.7	5.5	2.90	0.15	125	2.6
laboratory	pCO2=460 µatm	7.75	1.57	130.07	21.07	0.00495	0.00036	10.53	0.959	0.189	0.033	12.46	2.50	691.619	65.608	56.69	9.61	4.46	1.40	2.23	0.07	0.02	0.01	318.0	222.0	2.01	0.67	127	4.0																																										20	32	2243	2018	2241	1924	15	8.03	13.76	417.71	419.13	1839.62	164.62	2.60	4.09	8.20	8.53	259.01	259.89	1690.80	224.34	3.54	5.48
laboratory	pCO2=640 µatm	8.02	1.40	138.16	10.18	0.00483	0.00076	10.48	0.481	0.216	0.051	13.20	1.24	671.981	213.692	50.38	12.07	6.45	1.65	2.04	0.17	0.01	0.01	542.0	142.0	3.71	0.49	142	26.0																																										20	32	2329	2153	2367	2084	15	7.91	19.55	593.61	595.63	1996.96	136.49	2.16	3.38	8.12	11.39	345.82	346.99	1867.01	205.93	3.25	5.03
laboratory	pCO2=730 µatm	5.48	0.99	115.71	12.71	0.00401	0.00108	8.91	0.354	0.146	0.031	13.00	1.50	820.054	210.922	62.49	10.82	5.71	0.46	2.14	0.23	0.02	0.01	342.0	236.0	2.68	0.20	130	1.7																																										20	32	2306	2153	2304	2004	15	7.86	22.20	674.01	676.30	2009.12	121.68	1.92	3.04	8.06	13.17	399.89	401.25	1882.44	181.39	2.86	4.43
laboratory	pCO2=460 µatm																			2.23	0.07	0.02	0.01							12.74	1.41	3.7	0.1	0.507	0.006	0.292	0.035	25.16	3.00	7.29	0.07	99.87	3.80	102	18.02																										20	32	2243	2018	2241	1924	15	8.03	13.76	417.71	419.13	1839.62	164.62	2.60	4.09	8.20	8.53	259.01	259.89	1690.80	224.34	3.54	5.48
laboratory	pCO2=640 µatm																			2.04	0.17	0.01	0.01							9.55	1.65	3.9	0.2	0.513	0.010	0.413	0.059	18.65	3.47	7.56	0.47	83.88	7.90	115	17.66																										20	32	2329	2153	2367	2084	15	7.91	19.55	593.61	595.63	1996.96	136.49	2.16	3.38	8.12	11.39	345.82	346.99	1867.01	205.93	3.25	5.03
laboratory	pCO2=730 µatm																			2.14	0.23	0.02	0.01							11.49	0.46	3.8	0.2	0.510	0.015	0.329	0.013	22.53	0.32	7.40	0.26	70.77	2.91	91	12.00																										20	32	2306	2153	2304	2004	15	7.86	22.20	674.01	676.30	2009.12	121.68	1.92	3.04	8.06	13.17	399.89	401.25	1882.44	181.39	2.86	4.43
laboratory	Initial day																			5.92	0.41	0.52	0.13																							-22.3	0.08	0.20	0.10
laboratory	pCO2=460 µatm																			2.23	0.07	0.02	0.01																							-22.7	0.20	3.20	0.35																						20	32	2243	2018	2241	1924	15	8.03	13.76	417.71	419.13	1839.62	164.62	2.60	4.09	8.20	8.53	259.01	259.89	1690.80	224.34	3.54	5.48
laboratory	pCO2=640 µatm																			2.04	0.17	0.01	0.01																							-23.4	0.03	2.95	0.78																						20	32	2329	2153	2367	2084	15	7.91	19.55	593.61	595.63	1996.96	136.49	2.16	3.38	8.12	11.39	345.82	346.99	1867.01	205.93	3.25	5.03
laboratory	pCO2=730 µatm																			2.14	0.23	0.02	0.01																							-24.4	0.15	3.10	0.70																						20	32	2306	2153	2304	2004	15	7.86	22.20	674.01	676.30	2009.12	121.68	1.92	3.04	8.06	13.17	399.89	401.25	1882.44	181.39	2.86	4.43
laboratory	Initial day																			5.92	0.41	0.52	0.13																											1.004	0.218			12.046
laboratory	pCO2=460 µatm																			2.23	0.07	0.02	0.01																											0.996	0.167	0.119	0.005	8.414	2.614	1.067	1.382	0.127	1.382	0.127	0.047	0.005	0.015	0.003							20	32	2243	2018	2241	1924	15	8.03	13.76	417.71	419.13	1839.62	164.62	2.60	4.09	8.20	8.53	259.01	259.89	1690.80	224.34	3.54	5.48
laboratory	pCO2=640 µatm																			2.04	0.17	0.01	0.01																											1.293	0.186	0.096	0.018	13.992	1.273	0.436	1.133	0.292	1.133	0.292	0.028	0.009	0.011	0.002							20	32	2329	2153	2367	2084	15	7.91	19.55	593.61	595.63	1996.96	136.49	2.16	3.38	8.12	11.39	345.82	346.99	1867.01	205.93	3.25	5.03
laboratory	pCO2=730 µatm																			2.14	0.23	0.02	0.01																											1.214	0.164	0.108	0.011	11.226	1.444	0.195	0.871	0.119	0.871	0.119	0.036	0.005	0.011	0.001							20	32	2306	2153	2304	2004	15	7.86	22.20	674.01	676.30	2009.12	121.68	1.92	3.04	8.06	13.17	399.89	401.25	1882.44	181.39	2.86	4.43
laboratory	pCO2=460 µatm																			2.23	0.07	0.02	0.01																																										384521	31258	26590	4470	36651	6601	20	32	2243	2018	2241	1924	15	8.03	13.76	417.71	419.13	1839.62	164.62	2.60	4.09	8.20	8.53	259.01	259.89	1690.80	224.34	3.54	5.48
laboratory	pCO2=640 µatm																			2.04	0.17	0.01	0.01																																										454917	29456	26360	3360	25206	2662	20	32	2329	2153	2367	2084	15	7.91	19.55	593.61	595.63	1996.96	136.49	2.16	3.38	8.12	11.39	345.82	346.99	1867.01	205.93	3.25	5.03
laboratory	pCO2=730 µatm																			2.14	0.23	0.02	0.01																																										504535	23785	21280	1550	30696	4841	20	32	2306	2153	2304	2004	15	7.86	22.20	674.01	676.30	2009.12	121.68	1.92	3.04	8.06	13.17	399.89	401.25	1882.44	181.39	2.86	4.43