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Hofmann, Laurie C; Fink, Artur; Bischof, Kai; de Beer, Dirk (2016): Microsensor studies on Padina from a natural CO2 seep: implications of morphology on acclimation to low pH. PANGAEA, https://doi.org/10.1594/PANGAEA.860218, Supplement to: Hofmann, LC et al. (2015): Microsensor studies on Padina from a natural CO2 seep: implications of morphology on acclimation to low pH. Journal of Phycology, 51(6), 1106-1115, https://doi.org/10.1111/jpy.12347

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Abstract:
Low seawater pH can be harmful to many calcifying marine organisms, but the calcifying macroalgae Padina spp. flourish at natural submarine carbon dioxide seeps where seawater pH is low. We show that the microenvironment created by the rolled thallus margin of Padina australis facilitates supersaturation of CaCO3 and calcifi-cation via photosynthesis-induced elevated pH. Using microsensors to investigate oxygen and pH dynamics in the microenvironment of P. australis at a shallow CO2 seep, we found that, under saturating light, the pH inside the microenvironment (pHME) was higher than the external seawater (pHSW) at all pHSW levels investigated, and the difference (i.e., pHME-pHSW) increased with decreasing pHSW (0.9 units at pHSW 7.0). Gross photosynthesis (Pg) inside the microenvironment increased with decreasing pHSW, but algae from the control site reached a threshold at pH 6.5. Seep algae showed no pH threshold with respect to Pg within the pHSW range investigated. The external carbonic anhydrase (CA) inhibitor, acetazolamide, strongly inhibited Pg of P. australis at pHSW 8.2, but the effect was diminished under low pHSW (6.4-7.5), suggesting a greater dependence on membrane-bound CA for the dehydration of HCO3- ions during dissolved inorganic carbon uptake at the higher pHSW. In comparison, a calcifying green alga, Halimeda cuneata f. digitata, was not inhibited by AZ, suggesting efficient bicarbonate transport. The ability of P. australis to elevate pHME at the site of calcification and its strong dependence on CA may explain why it can thrive at low pHSW.
Keyword(s):
Benthos; Bottles or small containers/Aquaria ( 20 L); Chlorophyta; Chromista; Coast and continental shelf; Field experiment; Macroalgae; Ochrophyta; Padina australis; Primary production/Photosynthesis; Single species; South Pacific; Temperate
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloise (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.8. https://cran.r-project.org/package=seacarb
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2016-05-05.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1TypeTypeHofmann, Laurie Cstudy
2SpeciesSpeciesHofmann, Laurie C
3Registration number of speciesReg spec noHofmann, Laurie C
4Uniform resource locator/link to referenceURL refHofmann, Laurie CWoRMS Aphia ID
5FigureFigHofmann, Laurie C
6SiteSiteHofmann, Laurie C
7TreatmentTreatHofmann, Laurie C
8IdentificationIDHofmann, Laurie C
9Run Date/TimeRun Date/TimeHofmann, Laurie C
10DistanceDistanceµmHofmann, Laurie Cdepth from inside microenvironment
11OxygenO2µmol/lHofmann, Laurie C
12Oxygen, standard errorO2 std e±Hofmann, Laurie C
13DistanceDistanceµmHofmann, Laurie Cdepth from thallus surface
14OxygenO2µmol/lHofmann, Laurie Ccorrected for drift
15LocationLocationHofmann, Laurie C
16IdentificationIDHofmann, Laurie Cindividual
17ReplicateReplicateHofmann, Laurie C
18Gross photosynthesis rate, oxygenPG O2nmol/cm3/sHofmann, Laurie C
19Gross photosynthesis rate, oxygenPG O2nmol/cm3/sHofmann, Laurie Caverage
20SalinitySalHofmann, Laurie C
21Temperature, waterTemp°CHofmann, Laurie C
22pHpHHofmann, Laurie CPotentiometricfree scale
23Carbon, inorganic, dissolvedDICµmol/kgHofmann, Laurie C
24Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
25pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
26Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
27Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
28Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
29Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
30Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
31Alkalinity, totalATµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
32Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
33Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
Size:
40403 data points

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