Citation:

fsv259

Name: Effects of high CO2 levels on the ecophysiology of the diatom Thalassiosira weissflogii differ depending on the iron nutritional status
Published: 2016-04-06
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Micro-nutrients; North Pacific; Ochrophyta; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Thalassiosira weissflogii

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Abstract:

Iron availability in seawater, namely the concentration of dissolved inorganic iron ([Fe']), is affected by changes in pH. Such changes in the availability of iron should be taken into account when investigating the effects of ocean acidification on phytoplankton ecophysiology because iron plays a key role in phytoplankton metabolism. However, changes in iron availability in response to changes in ocean acidity are difficult to quantify specifically using natural seawater because these factors change simultaneously. In the present study, the availability of iron and carbonate chemistry were manipulated individually and simultaneously in the laboratory to examine the effect of each factor on phytoplankton ecophysiology. The effects of various pCO2 conditions (390, 600, and 800 µatm) on the growth, cell size, and elemental stoichiometry (carbon [C], nitrogen [N], phosphorus [P], and silicon [Si]) of the diatom Thalassiosira weissflogii under high iron ([Fe'] = 240 pmol/l) and low iron ([Fe'] = 24 pmol/l) conditions were investigated. Cell volume decreased with increasing pCO2, whereas intracellular C, N, and P concentrations increased with increasing pCO2 only under high iron conditions. Si:C, Si:N, and Si:P ratios decreased with increasing pCO2. It reflects higher production of net C, N, and P with no corresponding change in net Si production under high pCO2 and high iron conditions. In contrast, significant linear relationships between measured parameters and pCO2 were rarely detected under low iron conditions. We conclude that the increasing CO2 levels could affect on the biogeochemical cycling of bioelements selectively under the iron-replete conditions in the coastal ecosystems.

Keyword(s):

Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Micro-nutrients; North Pacific; Ochrophyta; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Thalassiosira weissflogii

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.8. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2016-04-01.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Sugie, Koji		study
2	Species	Species		Sugie, Koji
3	Registration number of species	Reg spec no		Sugie, Koji
4	Uniform resource locator/link to reference	URL ref		Sugie, Koji		WoRMS Aphia ID
5	Treatment	Treat		Sugie, Koji
6	Iron, dissolved, inorganic	Fe´ diss	pmol/l	Sugie, Koji
7	Growth rate	µ	1/day	Sugie, Koji		specific
8	Time in days	Time	days	Sugie, Koji
9	Alkalinity, total	AT	µmol/kg	Sugie, Koji
10	Carbon, inorganic, dissolved	DIC	µmol/kg	Sugie, Koji
11	Cell density	Cells	#/ml	Sugie, Koji
12	Cell biovolume	Cell biovol	µm³	Sugie, Koji
13	Surface area	SA	µm²	Sugie, Koji
14	Carbon, organic, particulate	POC	µmol/l	Sugie, Koji
15	Nitrogen, particulate	PN	µmol/l	Sugie, Koji
16	Phosphorus, particulate	PP	µmol/l	Sugie, Koji
17	Biogenic silica	bSiO2	µmol/l	Sugie, Koji
18	Chlorophyll a	Chl a	pmol/l	Sugie, Koji
19	Carbon, organic, particulate, per cell	POC/cell	pmol/#	Sugie, Koji
20	Nitrogen, particulate, per cell	PN/cell	pmol/#	Sugie, Koji
21	Phosphorus, organic, particulate, per cell	POP/cell	pmol/#	Sugie, Koji
22	Biogenic silica, per cell	bSiO2/cell	fmol/#	Sugie, Koji
23	Chlorophyll a per cell	Chl a/cell	pg/#	Sugie, Koji
24	Carbon, intracellular	C in	mol/l	Sugie, Koji
25	Nitrogen, intracellular	N in	mol/l	Sugie, Koji
26	Phosphorus, intracellular	P in	mol/l	Sugie, Koji
27	Silicon per surface area	Si in	mmol/m²	Sugie, Koji
28	Chlorophyll a, intracellular	Chl a in	g/l	Sugie, Koji
29	Particulate organic carbon production per cell	POC prod/cell	µmol/#/day	Sugie, Koji		net
30	Net nitrogen production rate	Net N prod	pmol/#/day	Sugie, Koji
31	Net phosphorus production	Net P prod	fmol/#/day	Sugie, Koji
32	Net silicon production	Net Si prod	pmol/#/day	Sugie, Koji
33	Chlorophyll a production per cell	Chl a prod/cell	pg/#/day	Sugie, Koji
34	Carbon/Nitrogen ratio	C/N		Sugie, Koji
35	Carbon/Phosphorus ratio	C/P		Sugie, Koji
36	Nitrogen/Phosphorus ratio	N/P		Sugie, Koji
37	Silicon/Nitrogen, molar ratio	Si/N		Sugie, Koji
38	Silicon/Carbon, molar ratio	Si/C		Sugie, Koji
39	Silicon/Phosphorus ratio	Si/P		Sugie, Koji
40	Salinity	Sal		Sugie, Koji
41	Temperature, water	Temp	°C	Sugie, Koji
42	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
43	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
44	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
45	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
46	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
47	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
48	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
49	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
50	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1179 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type	2 Species	3 Reg spec no	4 URL ref	5 Treat	6 Fe´ diss [pmol/l]	7 µ [1/day]	8 Time [days]	9 AT [µmol/kg]	10 DIC [µmol/kg]	11 Cells [#/ml]	12 Cell biovol [µm³]	13 SA [µm²]	14 POC [µmol/l]	15 PN [µmol/l]	16 PP [µmol/l]	17 bSiO2 [µmol/l]	18 Chl a [pmol/l]	19 POC/cell [pmol/#]	20 PN/cell [pmol/#]	21 POP/cell [pmol/#]	22 bSiO2/cell [fmol/#]	23 Chl a/cell [pg/#]	24 C in [mol/l]	25 N in [mol/l]	26 P in [mol/l]	27 Si in [mmol/m²]	28 Chl a in [g/l]	29 POC prod/cell [µmol/#/day]	30 Net N prod [pmol/#/day]	31 Net P prod [fmol/#/day]	32 Net Si prod [pmol/#/day]	33 Chl a prod/cell [pg/#/day]	34 C/N	35 C/P	36 N/P	37 Si/N	38 Si/C	39 Si/P	40 Sal	41 Temp [°C]	42 CSC flag	43 pH	44 CO2 [µmol/kg]	45 fCO2water_SST_wet [µatm]	46 pCO2water_SST_wet [µatm]	47 [HCO3]- [µmol/kg]	48 [CO3]2- [µmol/kg]	49 Omega Arg	50 Omega Cal
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	Fe1-1	4.36	0.13																																	34.2	20
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	Fe1-2	4.36	0.19																																	34.2	20
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	Fe2-1	8.33	0.23																																	34.2	20
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	Fe3-1	20.24	0.49																																	34.2	20
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	Fe3-2	20.24	0.48																																	34.2	20
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	Fe4-1	40.09	0.67																																	34.2	20
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	Fe4-2	40.09	0.79																																	34.2	20
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	Fe5-1	79.82	0.96																																	34.2	20
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	Fe5-2	79.82	1.02																																	34.2	20
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	Fe6-1	801.84	1.16																																	34.2	20
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	Fe6-2	801.84	1.16																																	34.2	20
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	LFe-386-1	22.68		0	2343.77	2088.62																														34.2	20	15	8.05	13.35	410.07	411.47	1890.22	185.05	2.89	4.45
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	LFe-386-2	23.32		0	2344.91	2086.32																														34.2	20	15	8.05	13.13	403.32	404.69	1885.90	187.29	2.93	4.51
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	LFe-614-1	24.84		0	2353.92	2160.79																														34.2	20	15	7.92	18.81	577.86	579.83	1995.61	146.37	2.29	3.52
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	LFe-614-2	24.42		0	2352.56	2161.50																														34.2	20	15	7.92	19.01	584.15	586.14	1997.43	145.06	2.27	3.49
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	LFe-795-1	25.03		0	2349.49	2194.78																														34.2	20	15	7.83	23.56	723.81	726.27	2048.13	123.09	1.92	2.96
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	LFe-795-2	24.82		0	2351.08	2197.32																														34.2	20	15	7.83	23.72	728.90	731.38	2051.02	122.57	1.91	2.95
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	LFe-386-1	26.17		3	2346.39	2073.61																														34.2	20	15	8.08	12.24	375.97	377.25	1864.91	196.46	3.07	4.73
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	LFe-386-2	26.03		3	2352.51	2079.81																														34.2	20	15	8.08	12.31	378.08	379.36	1870.88	196.62	3.07	4.73
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	LFe-614-1	26.25		3	2358.28	2158.53																														34.2	20	15	7.93	18.18	558.41	560.31	1989.77	150.58	2.35	3.62
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	LFe-614-2	25.55		3	2358.84	2162.21																														34.2	20	15	7.93	18.51	568.58	570.52	1995.03	148.67	2.32	3.58
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	LFe-795-1	27.02		3	2358.75	2194.96																														34.2	20	15	7.85	22.43	689.17	691.51	2043.80	128.73	2.01	3.10
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	LFe-795-2	26.51		3	2359.88	2198.22																														34.2	20	15	7.85	22.74	698.70	701.08	2047.99	127.49	1.99	3.07
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	LFe-386-1	30.26	0.43	10	2353.12	2062.52	8119	665.88	368.77	44.62	8.00	0.54	4.74	20854628.8	5.50	0.99	0.0665	584.35	2.57	8.25	1.48	0.10	1.58	3.86	0.00238	0.43	28.75	0.25	1.11	5.57	82.65	14.83	0.59	0.11	8.79	34.2	20	15	8.11	11.28	346.44	347.62	1843.01	208.23	3.25	5.01
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	LFe-386-2	31.20	0.44	10	2358.07	2063.28	9225	673.98	371.75	48.36	8.92	0.62	5.40	24069682.6	5.24	0.97	0.0672	585.87	2.61	7.78	1.43	0.10	1.58	3.87	0.00233	0.43	29.86	0.26	1.16	5.42	78.03	14.39	0.61	0.11	8.72	34.2	20	15	8.11	11.10	340.95	342.11	1841.05	211.13	3.30	5.08
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	LFe-614-1	27.35	0.46	10	2364.86	2160.87	9648	650.88	363.21	49.88	8.55	0.60	5.33	24822142.0	5.17	0.89	0.0621	552.28	2.57	7.94	1.36	0.10	1.52	3.95	0.00239	0.41	28.70	0.26	1.19	5.84	83.22	14.26	0.62	0.11	8.89	34.2	20	15	7.94	17.84	548.04	549.91	1989.62	153.41	2.40	3.69
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	LFe-614-2	26.24	0.42	10	2363.25	2164.01	7896	648.56	362.34	44.10	7.80	0.49	4.54	20489800.0	5.58	0.99	0.0616	575.33	2.60	8.61	1.52	0.10	1.59	4.00	0.00236	0.42	26.03	0.24	1.10	5.65	90.62	16.03	0.58	0.10	9.34	34.2	20	15	7.93	18.30	562.14	564.05	1995.30	150.42	2.35	3.62
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	LFe-795-1	28.86	0.48	10	2369.58	2199.07	14808	667.96	369.53	73.25	13.56	0.86	7.06	35310970.0	4.95	0.92	0.0580	477.00	2.38	7.41	1.37	0.09	1.29	3.57	0.00236	0.44	27.71	0.23	1.14	5.40	85.35	15.79	0.52	0.10	8.23	34.2	20	15	7.87	21.71	667.05	669.32	2044.30	133.06	2.08	3.20
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	LFe-795-2	28.13	0.48	10	2367.33	2199.78	14219	675.25	372.22	67.04	12.03	0.86	7.68	35516186.2	4.72	0.85	0.0606	540.46	2.50	7.41	1.37	0.09	1.45	3.70	0.00224	0.40	28.81	0.26	1.19	5.57	77.86	13.97	0.64	0.11	8.92	34.2	20	15	7.86	22.06	677.87	680.18	2046.50	131.22	2.05	3.16
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	HFe-386-1	172.82		0	2351.61	2110.70																														34.2	20	15	8.02	14.46	444.30	445.81	1920.02	176.22	2.75	4.24
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	HFe-386-2	163.96		0	2349.75	2115.34																														34.2	20	15	8.01	14.94	459.07	460.64	1928.36	172.03	2.69	4.14
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	HFe-614-1	226.46		0	2357.22	2162.35																														34.2	20	15	7.92	18.67	573.59	575.54	1996.14	147.54	2.30	3.55
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	HFe-614-2	218.45		0	2356.57	2165.98																														34.2	20	15	7.91	19.12	587.50	589.50	2001.97	144.89	2.26	3.49
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	HFe-795-1	219.16		0	2353.31	2201.20																														34.2	20	15	7.83	24.01	737.55	740.07	2055.53	121.67	1.90	2.93
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	HFe-795-2	222.61		0	2353.06	2199.12																														34.2	20	15	7.83	23.73	729.10	731.58	2052.65	122.73	1.92	2.95
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	HFe-386-1	216.74		2	2353.14	2084.50																														34.2	20	15	8.07	12.56	386.02	387.33	1877.91	194.03	3.03	4.67
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	HFe-386-2	224.14		2	2351.26	2078.56																														34.2	20	15	8.08	12.29	377.68	378.96	1869.69	196.58	3.07	4.73
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	HFe-614-1	235.35		2	2354.36	2155.13																														34.2	20	15	7.93	18.17	558.38	560.28	1986.81	150.14	2.35	3.61
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	HFe-614-2	231.39		2	2356.54	2159.26																														34.2	20	15	7.93	18.41	565.51	567.43	1991.85	149.00	2.33	3.59
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	HFe-795-1	241.12		2	2354.82	2191.62																														34.2	20	15	7.85	22.45	689.70	692.05	2040.91	128.26	2.00	3.09
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	HFe-795-2	232.75		2	2352.57	2193.58																														34.2	20	15	7.84	23.00	706.51	708.92	2044.88	125.70	1.96	3.03
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	HFe-386-1	315.24	0.93	6	2365.52	2049.81	11108	809.71	420.12	80.14	13.93	0.87	8.09	57565526.8	7.21	1.25	0.0783	728.09	5.18	8.91	1.55	0.10	1.73	6.40	0.00668	1.16	72.45	0.67	4.80	5.75	92.16	16.02	0.58	0.10	9.30	34.2	20	15	8.15	10.11	310.73	311.79	1814.63	225.07	3.52	5.42
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	HFe-386-2	328.17	0.92	6	2364.63	2043.61	10823	827.85	426.37	75.68	13.35	0.76	7.60	56721860.2	6.99	1.23	0.0701	702.18	5.24	8.45	1.49	0.08	1.65	6.33	0.00646	1.14	64.80	0.65	4.84	5.67	99.70	17.59	0.57	0.10	10.01	34.2	20	15	8.16	9.86	302.88	303.91	1805.24	228.51	3.57	5.50
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	HFe-614-1	289.56	0.89	6	2365.25	2141.94	8850	807.62	419.39	63.94	11.59	0.71	6.33	47806356.4	7.22	1.31	0.0801	714.71	5.40	8.95	1.62	0.10	1.70	6.69	0.00239	0.41	28.70	0.26	1.19	5.52	90.15	16.34	0.55	0.10	8.92	34.2	20	15	7.98	16.05	493.14	494.82	1960.38	165.51	2.59	3.98
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	HFe-614-2	270.57	0.91	6	2363.35	2148.21	8297	806.74	419.09	61.07	10.98	0.67	5.99	44933329.6	7.36	1.32	0.0802	721.66	5.42	9.12	1.64	0.10	1.72	6.71	0.00236	0.42	26.03	0.24	1.10	5.56	91.74	16.50	0.55	0.10	9.00	34.2	20	15	7.96	16.75	514.72	516.47	1971.14	160.32	2.50	3.86
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	HFe-795-1	269.74	0.93	6	2363.36	2187.68	8082	784.82	411.46	59.52	10.51	0.68	5.68	42493537.0	7.36	1.30	0.0840	703.22	5.26	9.38	1.66	0.11	1.71	6.70	0.00236	0.44	27.71	0.23	1.14	5.66	87.70	15.49	0.54	0.10	8.37	34.2	20	15	7.88	20.96	643.99	646.19	2030.72	136.00	2.12	3.27
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	HFe-795-2	281.72	0.94	6	2362.70	2182.29	10230	771.86	406.92	73.68	13.72	0.83	7.26	52366716.4	7.20	1.34	0.0810	709.77	5.12	9.33	1.74	0.10	1.74	6.63	0.00224	0.40	28.81	0.26	1.19	5.37	88.90	16.56	0.53	0.10	8.76	34.2	20	15	7.89	20.38	626.04	628.17	2023.07	138.85	2.17	3.34