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Knittel, Katrin; Boetius, Antje; Lemke, Andreas; Eilers, Heike; Lochte, Karin; Pfannkuche, Olaf; Linke, Peter; Amann, Rudolf (2016): Microbial community composition in sediments of the Hydrate Ridge (Cascadian Margin) collected during RV SONNE cruises SO143 (1999) and SO148-1 (2000). PANGAEA,, Supplement to: Knittel, K et al. (2003): Activity, distribution, and diversity of sulfate reducers and other bacteria in sediments above gas hydrate (Cascadia Margin, Oregon). Geomicrobiology Journal, 20(4), 269-294,

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Cold seep environments such as sediments above outcropping hydrate at Hydrate Ridge (Cascadia margin off Oregon) are characterized by methane venting, high sulfide fluxes caused by the anaerobic oxidation of methane, and the presence of chemosynthetic communities. This investigation deals with the diversity and distribution of sulfate-reducing bacteria, some of which are directly involved in the anaerobic oxidation of methane as syntrophic partners of the methanotrophic archaea. The composition and activity of the microbial communities at methane vented and nonvented sediments are compared by quantitative methods including total cell counts, fluorescence in situ hybridization (FISH). Bacteria involved in the degradation of particulate organic carbon (POC) are as active and diverse as at other productive margin sites of similar water depths. The availability of methane supports a two orders of magnitude higher microbial biomass (up to 9.6×10**10cells/cm**3). Sediment samples were obtained during RV SONNE cruises SO143-2 and SO148-1 at the crest of southern Hydrate Ridge at the Cascadia convergent margin off the coast of Oregon. Sediment cores of 20 - 40 cm length were obtained using a video-guided multiple corer from gas hydrate bearing sediments and from reference sites not enriched in methane in the surface sediments. Samples for total cell counts were obtained from 1 cm core slices, fixed with 2% formaldehyde and stored cold (4°C) and the quantification of aggregates was done via epifluorescence microscopy after staining the sediments with Acridine Orange Direct Counts (AODC) according to the method of Meyer- Reil (1983, doi:10.1007/BF00395813). Total cell counts were defined as the sum of single cells plus the aggregated cells in the syntrophic consortia. DAPI staining was used to measure ANME2/DSS aggregate sizes via epifluorescence microscopy of FISH-treated samples. For FISH, subsamples of sediment cores were sliced into 1 cm intervals and fixed for 2-3 h with 3% formaldehyde (final concentration), washed twice with 1×PBS (10 mM sodium phosphate; 130 mM NaCl), and finally stored in 1×PBS/EtOH (1:1) at -20°C.
Median Latitude: 44.569588 * Median Longitude: -125.146493 * South-bound Latitude: 44.568330 * West-bound Longitude: -125.147750 * North-bound Latitude: 44.570300 * East-bound Longitude: -125.139670
Date/Time Start: 1999-08-06T02:23:00 * Date/Time End: 2000-08-01T05:00:00
Minimum DEPTH, sediment/rock: 0.005 m * Maximum DEPTH, sediment/rock: 0.145 m
SO143_105-1 * Latitude Start: 44.569000 * Longitude Start: -125.146833 * Latitude End: 44.570300 * Longitude End: -125.147050 * Date/Time Start: 1999-08-06T02:23:00 * Date/Time End: 1999-08-06T03:27:00 * Elevation: -787.0 m * Campaign: SO143/2 (TECFLUX I) * Basis: Sonne * Method/Device: Multicorer with television (TVMUC)
SO143_139 * Latitude: 44.568330 * Longitude: -125.139670 * Date/Time: 1999-08-12T00:00:00 * Elevation: -830.0 m * Location: Cascadia Margin * Method/Device: Multicorer with television (TVMUC)
SO143_185-1 * Latitude Start: 44.569833 * Longitude Start: -125.147167 * Latitude End: 44.570033 * Longitude End: -125.147233 * Date/Time Start: 1999-08-18T19:43:00 * Date/Time End: 1999-08-18T20:35:00 * Elevation: -787.0 m * Campaign: SO143/2 (TECFLUX I) * Basis: Sonne * Method/Device: Multicorer with television (TVMUC)
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Event labelEventKnittel, Katrin
2Date/Time of eventDate/TimeKnittel, Katrin
3Latitude of eventLatitudeKnittel, Katrin
4Longitude of eventLongitudeKnittel, Katrin
5DEPTH, sediment/rockDepthmKnittel, KatrinGeocode
6Anaerobic methanotrophic archaea-2/DSS aggregates, targeted with ANME-2-538 and DSS658 oligonucleotide FISH-probeANME-2-538/DSS658 agg#/cm3Knittel, KatrinFluorescence in situ hybridization (FISH)
7Anaerobic methanotrophic archaea-2/DSS single cell, targeted with ANME-2-538 and DSS658 oligonucleotide FISH-probeANME-2-538/DSS658 cell s#/cm3Knittel, KatrinFluorescence in situ hybridization (FISH)
8Anaerobic methanotrophic archaea-2/DSS total cells, targeted with ANME-2-538 and DSS658 oligonucleotide FISH-probeANME-2-538/DSS658 cell tot#/cm3Knittel, KatrinFluorescence in situ hybridization (FISH)
9Anaerobic methanotrophic archaea-2/DSS, cells in aggregates, targeted with ANME-2-538 and DSS658 oligonucleotide FISH-probeANME-2-538/DSS658 cell in agg%Knittel, KatrinFluorescence in situ hybridization (FISH)
10Bacteria, targed with EUB338(I-III) oligonucleotide FISH-probeEUB338 I-III%Knittel, KatrinFluorescence in situ hybridization (FISH)
11Archaea, targed with ARCH915 oligonucleotide FISH-probeARCH915%Knittel, KatrinFluorescence in situ hybridization (FISH)
12Desulfusarcina/Desulfococcus, targeted with DSS658 oligonucleotide FISH-probeDSS658%Knittel, KatrinFluorescence in situ hybridization (FISH)
13Desulforhopalus spp., targeted with DSR651 oligonucleotides FISH-probeDSR651%Knittel, KatrinFluorescence in situ hybridization (FISH)
14Desulfovibrio spp., targeted with DSV698 oligonucleotides FISH-probeDSV698%Knittel, KatrinFluorescence in situ hybridization (FISH)
15Desulfobacterium spp., targeted with 221 oligonucleotides FISH-probeDSFB221%Knittel, KatrinFluorescence in situ hybridization (FISH)
16Desulfotalea spp., targeted with Sval428 oligonucleotides FISH-probeSval428%Knittel, KatrinFluorescence in situ hybridization (FISH)
17Desulfobulbus spp., targeted with 660 oligonucleotides FISH-probeDSFB660%Knittel, KatrinFluorescence in situ hybridization (FISH)
18Cytophaga-Flavobacterium cluster, targeted with CF319a oligonucleotide FISH-probeCF319a%Knittel, KatrinFluorescence in situ hybridization (FISH)
19HabitatHabitatKnittel, KatrinVisual description
665 data points

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