Citation:

eva.12370

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Abstract:

Transgenerational effects can buffer populations against environmental change, yet little is known about underlying mechanisms, their persistence, or the influence of environmental cue timing. We investigated mitochondrial respiratory capacity (MRC) and gene expression of marine sticklebacks that experienced acute or developmental acclimation to simulated ocean warming (21°C) across three generations. Previous work showed that acute acclimation of grandmothers to 21°C led to lower (optimised) offspring MRCs. Here, developmental acclimation of mothers to 21°C led to higher, but more efficient offspring MRCs. Offspring with a 21°Cx17°C grandmother-mother environment mismatch showed metabolic compensation: their MRCs were as low as offspring with a 17°C thermal history across generations. Transcriptional analyses showed primarily maternal but also grandmaternal environment effects: genes involved in metabolism and mitochondrial protein biosynthesis were differentially expressed when mothers developed at 21°C, whereas 21°C grandmothers influenced genes involved in hemostasis and apoptosis. Genes involved in mitochondrial respiration all showed higher expression when mothers developed at 21° and lower expression in the 21°Cx17°C group, matching the phenotypic pattern for MRCs. Our study links transcriptomics to physiology under climate change, and demonstrates that mechanisms underlying transgenerational effects persist across multiple generations with specific outcomes depending on acclimation type and environmental mismatch between generations.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Comment
1	Group	Group		Shama, Lisa N S
2	Gasterosteus aculeatus, acclimation temperature, maternal	G. aculeatus accl temp maternal	°C	Shama, Lisa N S	granddam
3	Gasterosteus aculeatus, acclimation temperature, maternal	G. aculeatus accl temp maternal	°C	Shama, Lisa N S	dam
4	Temperature, rearing	T rear	°C	Shama, Lisa N S	offspring rearing temperature
5	Temperature, rearing	T rear	°C	Shama, Lisa N S	mitochondrial respiration assay temperature
6	Replicates	Repl	#	Shama, Lisa N S	respiration assay replicate
7	Phosphorylation system capacity, maximum	OXPHOS max	pmol/s/mg	Shama, Lisa N S
8	Electron transport system capacity, maximum	ETS max	pmol/s/mg	Shama, Lisa N S
9	Phosphorylation inefficiency	LEAK	pmol/s/mg	Shama, Lisa N S
10	Net phosphorylation efficiency	P_Eff	pmol/s/mg	Shama, Lisa N S	calculated as 1-(leak/oxphos)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Size:

380 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Group	2 G. aculeatus accl temp maternal [°C] (granddam)	3 G. aculeatus accl temp maternal [°C] (dam)	4 T rear [°C] (offspring rearing temperature)	5 T rear [°C] (mitochondrial respiration ass...)	6 Repl [#] (respiration assay replicate)	7 OXPHOS max [pmol/s/mg]	8 ETS max [pmol/s/mg]	9 LEAK [pmol/s/mg]	10 P_Eff [pmol/s/mg] (calculated as 1-(leak/oxphos))
R1	17	17	17	17	1	56.7819	50.2135	12.5996	0.7781
R1	17	17	17	21	1	49.8619	60.3736	13.8275	0.7227
R1	17	17	17	17	2	33.6794	54.3661	4.4678	0.8673
R1	17	17	17	21	2	33.8406	65.7426	8.1647	0.7587
R1	17	17	17	17	3	47.2177	59.2026	9.2862	0.8033
R1	17	17	17	21	3	58.0904	74.3406	12.1372	0.7911
R2	17	17	21	17	1	55.6575	56.9555	15.1082	0.7286
R2	17	17	21	21	1	74.7894	74.8835	13.6418	0.8176
R2	17	17	21	17	2	40.2059	62.1796	7.5753	0.8116
R2	17	17	21	21	2	38.9525	70.9694	9.8873	0.7462
R2	17	17	21	17	3	41.6777	55.7878	9.0692	0.7824
R2	17	17	21	21	3	59.8566	100.1187	14.7523	0.7535
R3	21	17	17	17	1	39.0596	36.5405	11.8790	0.6959
R3	21	17	17	21	1	32.4104	23.0509	8.8551	0.7268
R3	21	17	17	17	2	47.0215	69.0179	10.3376	0.7802
R3	21	17	17	21	2	40.2242	71.8352	10.2539	0.7451
R3	21	17	17	17	3	41.7857	80.5646	9.4991	0.7727
R3	21	17	17	21	3	47.8944	69.9300	9.8085	0.7952
R4	21	17	21	17	1	46.8441	44.2815	8.5822	0.8168
R4	21	17	21	21	1	53.2009	63.9310	16.6047	0.6879
R4	21	17	21	17	2	46.7727	68.6130	10.3058	0.7797
R4	21	17	21	21	2	40.2350	87.0085	15.8094	0.6071
R4	21	17	21	17	3	40.0628	58.5057	8.7261	0.7822
R4	21	17	21	21	3	59.7814	99.4212	16.7966	0.7190
R5	17	21	17	17	1	58.2450	82.2432	14.5047	0.7510
R5	17	21	17	21	1	57.2962	82.0489	13.7977	0.7592
R5	17	21	17	17	2	44.1025	73.4135	9.7365	0.7792
R5	17	21	17	21	2	49.6384	86.8283	11.0026	0.7783
R5	17	21	17	17	3	46.4059	70.6093	11.8793	0.7440
R5	17	21	17	21	3	45.7281	73.9352	13.1578	0.7123
R6	17	21	21	17	1	74.2480	104.6834	11.6203	0.8435
R6	17	21	21	21	1	51.2962	69.5178	11.3466	0.7788
R6	17	21	21	17	2	56.3875	86.0742	13.0929	0.7678
R6	17	21	21	21	2	58.9212	105.0776	9.8921	0.8321
R6	17	21	21	17	3	43.6067	58.1547	7.8414	0.8202
R6	17	21	21	21	3	64.0374	112.7057	16.3300	0.7450
R7	21	21	17	17	1	59.0314	104.8367	13.9631	0.7635
R7	21	21	17	21	1	59.2945	145.7882	19.5313	0.6706