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Li, Wei; Gao, Kunshan; Beardall, John (2015): Nitrate limitation and ocean acidification interact with UV-B to reduce photosynthetic performance in the diatom [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.851339, Supplement to: Li, W et al. (2015): Nitrate limitation and ocean acidification interact with UV-B to reduce photosynthetic performance in the diatom Phaeodactylum tricornutum. Biogeosciences, 12(8), 2383-2393, https://doi.org/10.5194/bg-12-2383-2015

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Abstract:
It has been proposed that ocean acidification (OA) will interact with other environmental factors to influence the overall impact of global change on biological systems. Accordingly we investigated the influence of nitrogen limitation and OA on the physiology of diatoms by growing the diatom Phaeodactylum tricornutum Bohlin under elevated (1000 µatm; high CO2- HC) or ambient (390 µatm; low CO2-LC) levels of CO2 with replete (110 µmol/L; high nitrate-HN) or reduced (10 ?mol/L; low nitrate-LN) levels of NO3- and subjecting the cells to solar radiation with or without UV irradiance to determine their susceptibility to UV radiation (UVR, 280-400 nm). Our results indicate that OA and UVB induced significantly higher inhibition of both the photosynthetic rate and quantum yield under LN than under HN conditions. UVA or/and UVB increased the cells' non-photochemical quenching (NPQ) regardless of the CO2 levels. Under LN and OA conditions, activity of superoxide dismutase and catalase activities were enhanced, along with the highest sensitivity to UVB and the lowest ratio of repair to damage of PSII. HC-grown cells showed a faster recovery rate of yield under HN but not under LN conditions. We conclude therefore that nutrient limitation makes cells more prone to the deleterious effects of UV radiation and that HC conditions (ocean acidification) exacerbate this effect. The finding that nitrate limitation and ocean acidification interact with UV-B to reduce photosynthetic performance of the diatom P. tricornutum implies that ocean primary production and the marine biological C pump will be affected by OA under multiple stressors.
Keyword(s):
Bottles or small containers/Aquaria (<20 L); Charophyta; Chromista; Laboratory experiment; Laboratory strains; Light; Macro-nutrients; North Atlantic; Ochrophyta; Pelagos; Phaeodactylum tricornutum; Phytoplankton; Primary production/Photosynthesis; Single species
Related to:
Li, Wei; Gao, Kunshan; Beardall, John (2012): Interactive effects of ocean acidification and nitrogen limitation on the diatom Phaeodactylum tricornutum. PANGAEA, https://doi.org/10.1594/PANGAEA.823110
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.8. https://cran.r-project.org/package=seacarb
Project(s):
Ocean Acidification International Coordination Centre (OA-ICC)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2013-11-20.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1SpeciesSpeciesGao, Kunshan
2FigureFigGao, Kunshan
3TableTabGao, Kunshan
4TreatmentTreatGao, KunshanLC-HN=low pCO2 high NO3-, HC-HN=high CO2 high NO3-, LC-LN=low pCO2 low NO3-, LC-HN=low pCO2 high NO3-
5TreatmentTreatGao, Kunshan
6Photosynthetic carbon fixation rate, per cellC fix/cellpg/#/hGao, Kunshan
7Photosynthetic carbon fixation rate, standard deviationC fix std dev±Gao, Kunshan
8Photosynthetic carbon fixation rate, per chlorophyll aC fix/Chl aµg/µg/hGao, Kunshan
9Photosynthetic carbon fixation rate, standard deviationC fix std dev±Gao, Kunshan
10Ultraviolet-a radiation-induced inhibition of carbon fixationUV-a inhib C fix%Gao, Kunshan
11Ultraviolet-a radiation-induced inhibition of carbon fixation, standard deviationUV-a inhib C fix std dev±Gao, Kunshan
12Ultraviolet-b radiation-induced inhibition of carbon fixationUV-b inhib C fix%Gao, Kunshan
13Ultraviolet-b radiation-induced inhibition of carbon fixation, standard deviationUV-b inhib C fix std dev±Gao, Kunshan
14Ultraviolet-a radiation-induced inhibition of effective photochemical quantum yieldUV-a inhib Fv/Fm%Gao, Kunshan
15Ultraviolet-a radiation-induced inhibition of effective photochemical quantum yield, standard deviationUV-a inhib Fv/Fm std dev±Gao, Kunshan
16Ultraviolet-b radiation-induced inhibition of effective photochemical quantum yieldUV-b inhib Fv/Fm%Gao, Kunshan
17Ultraviolet-b radiation-induced inhibition of effective photochemical quantum yield, standard deviationUV-b inhib Fv/Fm std dev±Gao, Kunshan
18Time in minutesTimeminGao, Kunshan
19Non photochemical quenchingNPQGao, Kunshan
20Non photochemical quenching, standard deviationNPQ std dev±Gao, Kunshan
21Protein per cellProtein/cellpg/#Gao, Kunshan
22Proteins, standard deviationProtein std dev±Gao, Kunshan
23Superoxide dismutase activity, unit per protein massSOD/protU/mgGao, Kunshan
24Superoxide dismutase activity, standard deviationSOD std dev±Gao, Kunshan
25Superoxide dismutase activity, unit per cellSOD/cellU/#Gao, Kunshan
26Superoxide dismutase activity, standard deviationSOD std dev±Gao, Kunshan
27Catalase activity, unit per protein massCAT/protU/mgGao, Kunshan
28Catalase activity, standard deviationCAT std dev±Gao, Kunshan
29Catalase activity, unit per cellCAT/cellU/#Gao, Kunshan
30Catalase activity, standard deviationCAT std dev±Gao, Kunshan
31Time in minutesTimeminGao, Kunshanexposure
32Effective quantum yieldYGao, Kunshan
33Effective quantum yield, standard deviationY std dev±Gao, Kunshan
34Time in minutesTimeminGao, Kunshanrecovery
35Time in minutesTimeminGao, Kunshanthe recovery time to half maximal yield values
36Time, standard deviationTime std devGao, Kunshanthe recovery time to half maximal yield values
37Exponential rate constant for recoveryExponential rate const for rec1/minGao, Kunshan
38Exponential rate constant for recovery, standard deviationExponential rate const for rec std dev±Gao, Kunshan
39Repair rater1/minGao, Kunshan
40Repair rate, standard deviationr std dev±Gao, Kunshan
41Damage ratek1/minGao, Kunshan
42Damage rate, standard deviationk std dev±Gao, Kunshan
43Damage/repair ratiok/rGao, Kunshan
44Damage/repair ratio, standard deviationk/r standard deviation±Gao, Kunshan
45Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmGao, KunshanCalculated using CO2SYS
46Partial pressure of carbon dioxide, standard deviationpCO2 std dev±Gao, KunshanCalculated using CO2SYS
47pHpHGao, KunshanPotentiometricNBS scale
48pH, standard deviationpH std dev±Gao, KunshanPotentiometricNBS scale
49Carbon, inorganic, dissolvedDICµmol/kgGao, KunshanCoulometric titration
50Carbon, inorganic, dissolved, standard deviationDIC std dev±Gao, KunshanCoulometric titration
51Bicarbonate ion[HCO3]-µmol/kgGao, KunshanCalculated using CO2SYS
52Bicarbonate ion, standard deviation[HCO3]- std dev±Gao, KunshanCalculated using CO2SYS
53Carbonate ion[CO3]2-µmol/kgGao, KunshanCalculated using CO2SYS
54Carbonate ion, standard deviation[CO3]2- std dev±Gao, KunshanCalculated using CO2SYS
55Carbon dioxideCO2µmol/kgGao, KunshanCalculated using CO2SYS
56Carbon dioxide, standard deviationCO2 std dev±Gao, KunshanCalculated using CO2SYS
57Alkalinity, totalATµmol/kgGao, KunshanCalculated using CO2SYS
58Alkalinity, total, standard deviationAT std dev±Gao, KunshanCalculated using CO2SYS
59Temperature, waterTemp°CGao, Kunshan
60SalinitySalGao, Kunshan
61Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
62pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
63Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
64Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
65Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
66Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
67Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
68Alkalinity, total, standard deviationAT std dev±Yang, YanCalculated using seacarb after Nisumaa et al. (2010)
69Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
70Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
7864 data points

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