Citation:

cov018

Name: Altered neurotransmitter function in CO2-exposed stickleback (Gasterosteus aculeatus): a temperate model species for ocean acidification research
Published: 2015-09-15
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Animalia; Behaviour; Chordata; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Gasterosteus aculeatus; Growth/Morphology; Laboratory experiment; Nekton; North Atlantic; Pelagos; Single species; Temperate

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Abstract:

Studies on the consequences of ocean acidification for the marine ecosystem have revealed behavioural changes in coral reef fishes exposed to sustained near-future CO2 levels. The changes have been linked to altered function of GABAergic neurotransmitter systems, because the behavioural alterations can be reversed rapidly by treatment with the GABAA receptor antagonist gabazine. Characterization of the molecular mechanisms involved would be greatly aided if these can be examined in a well-characterized model organism with a sequenced genome. It was recently shown that CO2-induced behavioural alterations are not confined to tropical species, but also affect the three-spined stickleback, although an involvement of the GABAA receptor was not examined. Here, we show that loss of lateralization in the stickleback can be restored rapidly and completely by gabazine treatment. This points towards a worrying universality of disturbed GABAA function after high-CO2 exposure in fishes from tropical to temperate marine habitats. Importantly, the stickleback is a model species with a sequenced and annotated genome, which greatly facilitates future studies on underlying molecular mechanisms.

Keyword(s):

Animalia; Behaviour; Chordata; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Gasterosteus aculeatus; Growth/Morphology; Laboratory experiment; Nekton; North Atlantic; Pelagos; Single species; Temperate

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.8. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: 58.263020 * Longitude: 11.496920

Event(s):

Gullmars_Fjord * Latitude: 58.263020 * Longitude: 11.496920 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2015-09-15.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Species	Species		Lai, Floriana
2	Figure	Fig		Lai, Floriana
3	Treatment	Treat		Lai, Floriana
4	Duration, number of days	Duration	days	Lai, Floriana
5	Length	l	mm	Lai, Floriana		mean
6	Length, standard error	l std e	±	Lai, Floriana
7	Replicates	Repl	#	Lai, Floriana		length
8	Mass	Mass	g	Lai, Floriana		mean
9	Mass, standard error	Mass std e	±	Lai, Floriana
10	Replicates	Repl	#	Lai, Floriana		mass
11	Lateralization	Lat		Lai, Floriana		absolute lateralization index after 40 days of treatment
12	Lateralization, standard error	Lat std e	±	Lai, Floriana		absolute lateralization index after 40 days of treatment
13	Replicates	Repl	#	Lai, Floriana		absolute lateralization index after 40 days of treatment
14	Lateralization	Lat		Lai, Floriana		absolute lateralization index after 50 days of treatment
15	Lateralization, standard error	Lat std e	±	Lai, Floriana		absolute lateralization index after 50 days of treatment
16	Replicates	Repl	#	Lai, Floriana		absolute lateralization index after 50 days of treatment
17	Lateralization	Lat		Lai, Floriana		relative lateralization index after 40 days of treatment
18	Lateralization	Lat		Lai, Floriana		relative lateralization index after 50 days of treatment
19	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Lai, Floriana
20	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Lai, Floriana
21	Alkalinity, total	AT	µmol/kg	Lai, Floriana	Potentiometric titration
22	Alkalinity, total, standard deviation	AT std dev	±	Lai, Floriana	Potentiometric titration
23	Salinity	Sal		Lai, Floriana
24	Salinity, standard deviation	Sal std dev	±	Lai, Floriana
25	Temperature, water	Temp	°C	Lai, Floriana
26	Temperature, water, standard deviation	Temp std dev	±	Lai, Floriana
27	pH	pH		Lai, Floriana	Calculated using CO2calc	total scale
28	pH, standard deviation	pH std dev	±	Lai, Floriana	Calculated using CO2calc	total scale
29	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
30	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
31	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
32	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
33	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
34	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
35	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
36	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
37	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

2147 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Species	2 Fig	3 Treat	4 Duration [days]	5 l [mm] (mean)	6 l std e [±]	7 Repl [#] (length)	8 Mass [g] (mean)	9 Mass std e [±]	10 Repl [#] (mass)	11 Lat (absolute lateralization index...)	12 Lat std e [±] (absolute lateralization index...)	13 Repl [#] (absolute lateralization index...)	14 Lat (absolute lateralization index...)	15 Lat std e [±] (absolute lateralization index...)	16 Repl [#] (absolute lateralization index...)	17 Lat (relative lateralization index...)	18 Lat (relative lateralization index...)	19 pCO2water_SST_wet [µatm]	20 pCO2 std dev [±]	21 AT [µmol/kg] (Potentiometric titration)	22 AT std dev [±] (Potentiometric titration)	23 Sal	24 Sal std dev [±]	25 Temp [°C]	26 Temp std dev [±]	27 pH (total scale, Calculated using...)	28 pH std dev [±] (total scale, Calculated using...)	29 CSC flag (Calculated using seacarb afte...)	30 pH (total scale, Calculated using...)	31 CO2 [µmol/kg] (Calculated using seacarb afte...)	32 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	33 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	34 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	35 DIC [µmol/kg] (Calculated using seacarb afte...)	36 Omega Arg (Calculated using seacarb afte...)	37 Omega Cal (Calculated using seacarb afte...)
Gasterosteus aculeatus (fish)	S1, S2	Control	0	46.50000000	2.219234	16	1.304375000	0.2297280	16									442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	S1, S2	Control	10	47.33333333	2.185813	15	1.566000000	0.2501138	15									442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	S1, S2	Control	20	47.21428571	2.117895	14	1.447142857	0.2066117	14									442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	S1, S2	Control	50	52.18181818	1.016394	11	1.156363636	0.0815450	11									442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	S1, S2	CO2	0	46.18750000	1.686265	16	1.142500000	0.1497456	16									992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	S1, S2	CO2	10	47.00000000	1.783522	15	1.408666667	0.1892790	15									992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	S1, S2	CO2	20	47.92307692	1.913051	13	1.580000000	0.2520735	13									992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	S1, S2	CO2	50	52.08333333	1.322637	12	1.390833333	0.2251345	12									992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	2	Control								33.33	7.213	12						442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	2	CO2								13.85	5.005	13						992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	3	Control											36.36	5.270	11			442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	3	Control after gabazine											34.55	5.619	11			442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	3	CO2											16.67	3.761	12			992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	3	CO2 after gabazine											34.17	6.332	12			992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	2	Control														-70		442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	2	Control														-60		442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	2	Control														40		442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	2	Control														-10		442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	2	Control														-20		442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	2	Control														-30		442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	2	Control														-50		442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	2	Control														-60		442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	2	Control														10		442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	2	Control														0		442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	2	Control														0		442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	2	Control														50		442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	2	CO2														-30		992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	2	CO2														-10		992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	2	CO2														-20		992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	2	CO2														0		992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	2	CO2														10		992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	2	CO2														-60		992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	2	CO2														-20		992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	2	CO2														-30		992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	2	CO2														0		992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	2	CO2														0		992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	2	CO2														0		992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	2	CO2														0		992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	2	CO2														0		992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	3	Control															-60	442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	3	Control															-10	442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	3	Control															-50	442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	3	Control															-20	442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	3	Control															40	442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	3	Control															-20	442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	3	Control															-40	442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	3	Control																442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	3	Control															60	442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	3	Control															20	442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	3	Control															-50	442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	3	Control															-30	442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	3	Control																442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	3	Control after gabazine															-30	442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	3	Control after gabazine															-70	442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	3	Control after gabazine															0	442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	3	Control after gabazine															40	442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	3	Control after gabazine															40	442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	3	Control after gabazine															30	442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	3	Control after gabazine															30	442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	3	Control after gabazine																442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	3	Control after gabazine															20	442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	3	Control after gabazine															-50	442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	3	Control after gabazine															-50	442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	3	Control after gabazine															-20	442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	3	Control after gabazine																442.4	71.0	2205	239	25.9	2.3	13.1	2.4	8.02	0.052	24	8.02	18.43	440.76	1935.45	111.75	2065.63	1.75	2.81
Gasterosteus aculeatus (fish)	3	CO2															-10	992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	3	CO2															20	992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	3	CO2															40	992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	3	CO2																992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	3	CO2															-10	992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	3	CO2															0	992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	3	CO2															-20	992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	3	CO2															-40	992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	3	CO2															20	992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	3	CO2															-20	992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	3	CO2															40	992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	3	CO2															-10	992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	3	CO2															0	992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	3	CO2 after gabazine															50	992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	3	CO2 after gabazine															-60	992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	3	CO2 after gabazine															60	992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	3	CO2 after gabazine																992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	3	CO2 after gabazine															-30	992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	3	CO2 after gabazine															-40	992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	3	CO2 after gabazine															-20	992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	3	CO2 after gabazine															-10	992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	3	CO2 after gabazine															0	992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	3	CO2 after gabazine															-20	992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	3	CO2 after gabazine															-30	992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	3	CO2 after gabazine															-20	992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36
Gasterosteus aculeatus (fish)	3	CO2 after gabazine															-70	992.1	119.3	2149	195	25.9	2.3	13.1	2.4	7.69	0.057	24	7.69	41.33	988.43	2017.59	54.15	2113.08	0.85	1.36