Osterholz, Helena; Singer, Gabriel; Wemheuer, Bernd; Daniel, Rolf; Simon, Meinhard; Niggemann, Jutta; Dittmar, Thorsten (2016): Dissolved organic matter composition derived from FT-ICR-MS analyses during HEINCKE cruise HE361 [dataset publication series]. PANGAEA, https://doi.org/10.1594/PANGAEA.848886, Supplement to: Osterholz, H et al. (2016): Deciphering associations between dissolved organic molecules and bacterial communities in a pelagic marine system. The ISME Journal, https://doi.org/10.1038/ismej.2015.231
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Abstract:
Dissolved organic matter (DOM) is the main substrate and energy source for heterotrophic bacterioplankton. To understand the interactions between DOM and the bacterial community (BC), it is important to identify the key factors on both sides in detail, chemically distinct moieties in DOM and the various bacterial taxa. Next-generation sequencing facilitates the classification of millions of reads of environmental DNA and RNA amplicons and ultrahigh-resolution mass spectrometry yields up to 10,000 DOM molecular formulae in a marine water sample. Linking this detailed biological and chemical information is a crucial first step toward a mechanistic understanding of the role of microorganisms in the marine carbon cycle. In this study, we interpreted the complex microbiological and molecular information via a novel combination of multivariate statistics. We were able to reveal distinct relationships between the key factors of organic matter cycling along a latitudinal transect across the North Sea. Total BC and DOM composition were mainly driven by mixing of distinct water masses and presumably retain their respective terrigenous imprint on similar timescales on their way through the North Sea. The active microbial community, however, was rather influenced by local events and correlated with specific DOM molecular formulae indicative of compounds that are easily degradable. These trends were most pronounced on the highest resolved level, that is, operationally defined 'species', reflecting the functional diversity of microorganisms at high taxonomic resolution.
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Coverage:
Median Latitude: 57.334637 * Median Longitude: 5.751835 * South-bound Latitude: 54.089300 * West-bound Longitude: 3.036000 * North-bound Latitude: 59.984830 * East-bound Longitude: 9.000830
Date/Time Start: 2011-07-12T12:00:00 * Date/Time End: 2011-07-27T14:00:00
Comment:
Sequence data were deposited in the Sequence Read Archive (SRA) of the National Center for Biotechnology Information (NCBI) under the accession number SRA082674.
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
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2 datasets
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Datasets listed in this publication series
- Osterholz, H; Singer, G; Wemheuer, B et al. (2016): Dissolved organic matter composition derived from FT-ICR-MS analyses. https://doi.org/10.1594/PANGAEA.848881
- Osterholz, H; Singer, G; Wemheuer, B et al. (2016): Dissolved organic matter composition given as FT-ICR-MS peak intensities measured on water bottle samples during HEINCKE cruise HE361. https://doi.org/10.1594/PANGAEA.848880