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Mu, J; Jin, F; Wang, J Y; Zheng, N; Cong, Y (2015): Effects of CO2-driven ocean acidification on early life stages of marine medaka (Oryzias melastigma) [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.848420, Supplement to: Mu, J et al. (2015): Effects of CO2-driven ocean acidification on early life stages of marine medaka (Oryzias melastigma). Biogeosciences, 12(12), 3861-3868, https://doi.org/10.5194/bg-12-3861-2015

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Abstract:
The potential effects of elevated CO2 level and reduced carbonate saturation state in marine environment on fishes and other non-calcified organisms are still poorly known. In present study, we investigated the effects of ocean acidification on embryogenesis and organogenesis of newly hatched larvae of marine medaka (Oryzias melastigma) after 21 d exposure of eggs to different artificially acidified seawater (pH 7.6 and 7.2, respectively), and compared with those in control group (pH 8.2). Results showed that CO2-driven seawater acidification (pH 7.6 and 7.2) had no detectable effect on hatching time, hatching rate, and heart rate of embryos. However, the deformity rate of larvae in pH 7.2 treatment was significantly higher than that in control treatment. The left and right sagitta areas did not differ significantly from each other in each treatment. However, the mean sagitta area of larvae in pH 7.6 treatment was significantly smaller than that in the control (p = 0.024). These results suggest that although marine medaka might be more tolerant of elevated CO2 than some other fishes, the effect of elevated CO2 level on the calcification of otolith is likely to be the most susceptibly physiological process of pH regulation in early life stage of marine medaka.
Keyword(s):
Animalia; Behaviour; Bottles or small containers/Aquaria (<20 L); Chordata; Development; Growth/Morphology; Laboratory experiment; Laboratory strains; Mortality/Survival; Nekton; Not applicable; Oryzias melastigma; Pelagos; Reproduction; Single species
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.6. https://cran.r-project.org/package=seacarb
Project(s):
Ocean Acidification International Coordination Centre (OA-ICC)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2015-07-16.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1 FigureFigWang, J Y
2 SpeciesSpeciesWang, J Y
3 TreatmentTreatWang, J Y
4 Hatching timeHatching timedaysWang, J Yembryonic
5 Hatching time, standard deviationHatching time std dev±Wang, J Yembryonic
6 Hatching rateHatching%Wang, J Yembryonic
7 Hatching rate, standard deviationHatching std dev±Wang, J Yembryonic
8 Heart rateheart ratebeat/minWang, J Yembryonic
9 Heart rate, standard deviationHeart rate std dev±Wang, J Yembryonic
10 Larval deformity rateLarval def rate%Wang, J Y
11 Larval deformity rate, standard deviationLarval def rate std dev±Wang, J Y
12 SurvivalSurvival%Wang, J Ylarval
13 Survival rate, standard deviationSurvival rate std dev±Wang, J Ylarval
14 Otolith areaOtolith areamm2Wang, J Yleft
15 Otolith area, standard deviationOtolith area std dev±Wang, J Yleft
16 Otolith areaOtolith areamm2Wang, J Yright
17 Otolith area, standard deviationOtolith area std dev±Wang, J Yright
18 Otolith areaOtolith areamm2Wang, J Yavreage
19 Otolith area, standard deviationOtolith area std dev±Wang, J Yavreage
20 pHpHWang, J YPotentiometricNBS scale
21 pH, standard deviationpH std dev±Wang, J YPotentiometricNBS scale
22 Carbon, inorganic, dissolvedDICµmol/kgWang, J YCoulometric titration
23 Carbon, inorganic, dissolved, standard deviationDIC std dev±Wang, J YCoulometric titration
24 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmWang, J YCalculated using CO2SYS
25 Partial pressure of carbon dioxide, standard deviationpCO2 std dev±Wang, J YCalculated using CO2SYS
26 Carbon dioxideCO2µmol/kgWang, J YCalculated using CO2SYS
27 Carbon dioxide, standard deviationCO2 std dev±Wang, J YCalculated using CO2SYS
28 Bicarbonate ion[HCO3]-µmol/kgWang, J YCalculated using CO2SYS
29 Bicarbonate ion, standard deviation[HCO3]- std dev±Wang, J YCalculated using CO2SYS
30 Carbonate ion[CO3]2-µmol/kgWang, J YCalculated using CO2SYS
31 Carbonate ion, standard deviation[CO3]2- std dev±Wang, J YCalculated using CO2SYS
32 Aragonite saturation stateOmega ArgWang, J YCalculated using CO2SYS
33 Aragonite saturation state, standard deviationOmega Arg std dev±Wang, J YCalculated using CO2SYS
34 Temperature, waterTemp°CWang, J Y
35 Temperature, water, standard deviationTemp std dev±Wang, J Y
36 SalinitySalWang, J Y
37 Salinity, standard deviationSal std dev±Wang, J Y
38 OxygenO2µmol/kgWang, J Ydissolved
39 Oxygen, standard deviationO2 std dev±Wang, J Ydissolved
40 Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
41 pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
42 Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
43 Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
44 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
45 Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
46 Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
47 Alkalinity, totalATµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
48 Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
49 Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
345 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 
Fig		2 
Species		3 
Treat		4 
Hatching time [days]
(embryonic)		5 
Hatching time std dev [±]
(embryonic)		6 
Hatching [%]
(embryonic)		7 
Hatching std dev [±]
(embryonic)		8 
heart rate [beat/min]
(embryonic)		9 
Heart rate std dev [±]
(embryonic)		10 
Larval def rate [%]		11 
Larval def rate std dev [±]		12 
Survival [%]
(larval)		13 
Survival rate std dev [±]
(larval)		14 
Otolith area [mm2]
(left)		15 
Otolith area std dev [±]
(left)		16 
Otolith area [mm2]
(right)		17 
Otolith area std dev [±]
(right)		18 
Otolith area [mm2]
(avreage)		19 
Otolith area std dev [±]
(avreage)		20 
pH
(NBS scale, Potentiometric)		21 
pH std dev [±]
(NBS scale, Potentiometric)		22 
DIC [µmol/kg]
(Coulometric titration)		23 
DIC std dev [±]
(Coulometric titration)		24 
pCO2water_SST_wet [µatm]
(Calculated using CO2SYS)		25 
pCO2 std dev [±]
(Calculated using CO2SYS)		26 
CO2 [µmol/kg]
(Calculated using CO2SYS)		27 
CO2 std dev [±]
(Calculated using CO2SYS)		28 
[HCO3]- [µmol/kg]
(Calculated using CO2SYS)		29 
[HCO3]- std dev [±]
(Calculated using CO2SYS)		30 
[CO3]2- [µmol/kg]
(Calculated using CO2SYS)		31 
[CO3]2- std dev [±]
(Calculated using CO2SYS)		32 
Omega Arg
(Calculated using CO2SYS)		33 
Omega Arg std dev [±]
(Calculated using CO2SYS)		34 
Temp [°C]		35 
Temp std dev [±]		36 
Sal		37 
Sal std dev [±]		38 
O2 [µmol/kg]
(dissolved)		39 
O2 std dev [±]
(dissolved)		40 
CSC flag
(Calculated using seacarb afte...)		41 
pH
(total scale, Calculated using...)		42 
CO2 [µmol/kg]
(Calculated using seacarb afte...)		43 
fCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)		44 
pCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)		45 
[HCO3]- [µmol/kg]
(Calculated using seacarb afte...)		46 
[CO3]2- [µmol/kg]
(Calculated using seacarb afte...)		47 
AT [µmol/kg]
(Calculated using seacarb afte...)		48 
Omega Arg
(Calculated using seacarb afte...)		49 
Omega Cal
(Calculated using seacarb afte...)
3Oryzias melastigma (fish)pH=8.212.31.684.65.683.24.28.220.0042645.128.5495.92.214.40.12380.310.1280.43.94.50.06027.30.1030.80.07175.0000.9375278.0713.79503.03504.602336.60294.713018.554.857.38
3Oryzias melastigma (fish)pH=7.613.82.480.44.779.85.87.630.0073014.274.32372.652.368.71.42861.020.784.50.31.40.00627.50.0731.60.80171.8752.1875277.4864.702382.242389.662854.3495.153074.081.562.37
3Oryzias melastigma (fish)pH=7.215.23.485.112.484.69.97.220.0023202.718.56165.756.4178.41.82988.89.335.50.60.60.01027.10.2031.70.10175.0001.5625277.08174.876379.506399.542989.4238.403077.990.630.95
4Oryzias melastigma (fish)pH=8.210.22.675.79.28.220.0042645.128.5495.92.214.40.12380.310.1280.43.94.50.06027.30.1030.80.07175.0000.9375278.0713.79503.03504.602336.60294.713018.554.857.38
4Oryzias melastigma (fish)pH=7.612.12.966.224.17.630.0073014.274.32372.652.368.71.42861.020.784.50.31.40.00627.50.0731.60.80171.8752.1875277.4864.702382.242389.662854.3495.153074.081.562.37
4Oryzias melastigma (fish)pH=7.223.31.766.420.97.220.0023202.718.56165.756.4178.41.82988.89.335.50.60.60.01027.10.2031.70.10175.0001.5625277.08174.876379.506399.542989.4238.403077.990.630.95
6Oryzias melastigma (fish)pH=8.20.00751590.00099050.00733120.00093070.00742380.00095968.220.0042645.128.5495.92.214.40.12380.310.1280.43.94.50.06027.30.1030.80.07175.0000.9375278.0713.79503.03504.602336.60294.713018.554.857.38
6Oryzias melastigma (fish)pH=7.60.00691890.00106530.00689290.00107290.00690830.00109437.630.0073014.274.32372.652.368.71.42861.020.784.50.31.40.00627.50.0731.60.80171.8752.1875277.4864.702382.242389.662854.3495.153074.081.562.37
6Oryzias melastigma (fish)pH=7.20.00684560.00126420.00690480.00109430.00703270.00120317.220.0023202.718.56165.756.4178.41.82988.89.335.50.60.60.01027.10.2031.70.10175.0001.5625277.08174.876379.506399.542989.4238.403077.990.630.95