Not logged in
PANGAEA.
Data Publisher for Earth & Environmental Science

Wei, Lei; Wang, Qing; Ning, Xuanxuan; Mu, Changkao; Wang, Chunlin; Cao, Ruiwen; Wu, Huifeng; Cong, Ming; Li, Fei; Ji, Chenglong; Zhao, Jianmin (2015): Combined metabolome and proteome analysis of the mantle tissue from Pacific oyster Crassostrea gigas exposed to elevated pCO2 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.846883, Supplement to: Wei, L et al. (2015): Combined metabolome and proteome analysis of the mantle tissue from Pacific oyster Crassostrea gigas exposed to elevated pCO2. Comparative Biochemistry and Physiology Part D: Genomics & Proteomics, 13, 16-23, https://doi.org/10.1016/j.cbd.2014.12.001

Always quote citation above when using data! You can download the citation in several formats below.

RIS CitationBibTeX Citation

Abstract:
Ocean acidification (OA) has been found to affect an array of normal physiological processes in mollusks, especially posing a significant threat to the fabrication process of mollusk shell. In the current study, the impact of exposure to elevated pCO2 condition was investigated in mantle tissue of Crassostrea gigas by an integrated metabolomic and proteomic approach. Analysis of metabolome and proteome revealed that elevated pCO2 could affect energy metabolism in oyster C. gigas, marked by differentially altered ATP, succinate, MDH, PEPCK and ALDH levels. Moreover, the up-regulated calponin-2, tropomyosins and myosin light chains indicated that elevated pCO2 probably caused disturbances in cytoskeleton structure in mantle tissue of oyster C. gigas. This work demonstrated that a combination of proteomics and metabolomics could provide important insights into the effects of OA at molecular levels.
Keyword(s):
Animalia; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Crassostrea gigas; Gene expression (incl. proteomics); Laboratory experiment; Mollusca; Single species; South Pacific; Temperate
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.6. https://cran.r-project.org/package=seacarb
Project(s):
Ocean Acidification International Coordination Centre (OA-ICC)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2015-06-01.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1SpeciesSpeciesWu, Huifeng
2TreatmentTreatWu, Huifeng
3Gene nameGeneWu, Huifeng
4mRNA gene expression, relativemRNA expressWu, Huifeng
5mRNA gene expression, relative, standard deviationmRNA express std dev±Wu, Huifeng
6Temperature, waterTemp°CWu, Huifeng
7Temperature, water, standard deviationTemp std dev±Wu, Huifeng
8SalinitySalWu, Huifeng
9Salinity, standard deviationSal std dev±Wu, Huifeng
10pHpHWu, HuifengNBS scale
11pH, standard deviationpH std dev±Wu, HuifengNBS scale
12Carbon, inorganic, dissolvedDICµmol/kgWu, Huifeng
13Carbon, inorganic, dissolved, standard deviationDIC std dev±Wu, Huifeng
14Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetppmvWu, HuifengCalculated using CO2SYS
15Partial pressure of carbon dioxide, standard deviationpCO2 std dev±Wu, HuifengCalculated using CO2SYS
16Alkalinity, totalATµmol/kgWu, HuifengCalculated using CO2SYS
17Alkalinity, total, standard deviationAT std dev±Wu, HuifengCalculated using CO2SYS
18Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
19pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
20Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
21Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
22Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
23Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
24Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
25Alkalinity, totalATµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
26Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
27Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
540 data points

Download Data

Download dataset as tab-delimited text — use the following character encoding:

View dataset as HTML