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Schiffer, Melanie (2015): Temperature tolerance of different larval stages of the spider crab Hyas araneus exposed to elevated seawater PCO2. PANGAEA, https://doi.org/10.1594/PANGAEA.846762, Supplement to: Schiffer, Melanie; Harms, Lars; Lucassen, Magnus; Mark, Felix Christopher; Pörtner, Hans-Otto; Storch, Daniela (2014): Temperature tolerance of different larval stages of the spider crab Hyas araneus exposed to elevated seawater PCO2. Frontiers in Zoology, 11(1), https://doi.org/10.1186/s12983-014-0087-4

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Abstract:
Exposure to elevated seawater PCO2 limits the thermal tolerance of crustaceans but the underlying mechanisms have not been comprehensively explored. Larval stages of crustaceans are even more sensitive to environmental hypercapnia and possess narrower thermal windows than adults. In a mechanistic approach, we analysed the impact of high seawater CO2 on parameters at different levels of biological organization, from the molecular to the whole animal level. At the whole animal level we measured oxygen consumption, heart rate and activity during acute warming in zoea and megalopa larvae of the spider crab Hyas araneus exposed to different levels of seawater PCO2. Furthermore, the expression of genes responsible for acid-base regulation and mitochondrial energy metabolism, and cellular responses to thermal stress (e.g. the heat shock response) was analysed before and after larvae were heat shocked byrapidly raising the seawater temperature from 10°C rearing temperature to 20°C. Zoea larvae showed a high heat tolerance, which decreased at elevated seawater PCO2, while the already low heat tolerance of megalopa larvae was not limited further by hypercapnic exposure. There was a combined effect of elevated seawater CO2 and heat shock in zoea larvae causing elevated transcript levels of heat shock proteins. In all three larval stages, hypercapnic exposure elicited an up-regulation of genes involved in oxidative phosphorylation, which was, however, not accompanied by increased energetic demands. The combined effect of seawater CO2 and heat shock on the gene expression of heat shock proteins reflects the downward shift in thermal limits seen on the whole animal level and indicates an associated capacity to elicit passive thermal tolerance. The up-regulation of genes involved in oxidative phosphorylation might compensate for enzyme activities being lowered through bicarbonate inhibition and maintain larval standard metabolic rates at high seawater CO2 levels. The present study underlines the necessity to align transcriptomic data with physiological responses when addressing mechanisms affected by an interaction of elevated seawater PCO2 and temperature extremes.
Keyword(s):
Animalia; Arthropoda; Behaviour; Bottles or small containers/Aquaria ( 20 L); Coast and continental shelf; Gene expression (incl. proteomics); Hyas araneus; Laboratory experiment; Mortality/Survival; North Atlantic; Pelagos; Respiration; Single species; Temperate; Temperature; Zooplankton
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloise (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.6. https://cran.r-project.org/package=seacarb
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2015-06-04.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1SpeciesSpeciesSchiffer, Melanie
2TableTabSchiffer, Melanie
3FigureFigSchiffer, Melanie
4Day of experimentDOEdaySchiffer, Melanie
5Temperature, waterTemp°CSchiffer, Melanie
6TreatmentTreatSchiffer, Melanie
7StageStageSchiffer, Melanie
8Oxygen consumptionO2 conmg/kg/hSchiffer, Melanie
9Heart beat rateHeart beat#/minSchiffer, Melanie
10Heart rateheart ratebeat/minSchiffer, MelanieMaxiliped
11MortalityMortality%Schiffer, Melanie
12Gene nameGeneSchiffer, Melanie
13Gene expressionGene expressionSchiffer, Melaniefunction in cellular stress/heat shock response
14Standard deviationStd dev±Schiffer, MelanieGene expresseion function in cellular stress/heat shock response
15Gene expressionGene expressionSchiffer, Melaniefunction in acid-base regulation
16Standard deviationStd dev±Schiffer, MelanieGene expression function in acid-base regulation
17Gene expressionGene expressionSchiffer, Melaniefunction in mitochondrial energy metabolism
18Standard deviationStd dev±Schiffer, MelanieGene expression function in mitochondrial energy metabolism
19Temperature, waterTemp°CSchiffer, Melanieincubation
20Temperature, water, standard deviationTemp std dev±Schiffer, Melanieincubation
21pHpHSchiffer, Melanietotal scale
22pH, standard deviationpH std dev±Schiffer, Melanietotal scale
23Carbon, inorganic, dissolvedDICµmol/kgSchiffer, Melanie
24Carbon, inorganic, dissolved, standard deviationDIC std dev±Schiffer, Melanie
25Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmSchiffer, Melanie
26Partial pressure of carbon dioxide, standard deviationpCO2 std dev±Schiffer, Melanie
27SalinitySalSchiffer, Melanie
28Salinity, standard deviationSal std dev±Schiffer, Melanie
29Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
30Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
31Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
32Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
33Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
34Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
35Alkalinity, totalATµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
36Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
37Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
Size:
24150 data points

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