Citation:

PANGAEA.846494

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Abstract:

This work aimed to explore evaluated the effects of the increased of hydrostatic pressure on a defined bacterial community on aggregates formed from an axenic culture of marine diatoms by simulating sedimentation to the deep sea by increase of hydrostatic pressure up to 30 bar (equivalent to 3000 m water depth) against control at ambient surface pressure. Our hypothesis was that microbial colonization and community composition and thus microbial OM turnover is greatly affected by changes in hydrostatic pressure during sinking to the deep ocean.

Project(s):

Biological Impacts of Ocean Acidification (BIOACID)

Comment:

Determination of Organic and Inorganic Carbon Content and Nitrogen content

Aggregate samples were concentrated onto precombusted Whatman GF/F glass filters and analyzed for organic carbon. The filters were dried at 60°C overnight. After weighting, a section of each filter was placed into a silver cup and decarbonated with 1 M HCl to determine the organic carbon content. The samples were dried at 60°C and the process repeated until bubbling stopped. The cups were closed and compacted into spheres. These samples were analyzed with a Euro-EA Elemental analyzer (Hekatech) standardized with acetanilide. The inorganic carbon content was determined from the difference between the total carbon content and the organic carbon content.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Comment
1	Experimental treatment	Exp treat		Mendes, Pedro André	Pressure
2	Time in hours	Time	h	Mendes, Pedro André
3	pH	pH		Mendes, Pedro André
4	Particle concentration	Part conc	mg/l	Mendes, Pedro André
5	Nitrogen, total	TN	%	Mendes, Pedro André
6	Carbon, total	TC	%	Mendes, Pedro André
7	Carbon, organic, particulate	POC	%	Mendes, Pedro André
8	Carbon, organic, particulate	POC	mg/l	Mendes, Pedro André
9	Carbon, inorganic, particulate	PIC	%	Mendes, Pedro André
10	Carbon/Nitrogen ratio	C/N		Mendes, Pedro André
11	Size	Size	µm	Mendes, Pedro André	median size
12	Sinking velocity	Sinking V	m/day	Mendes, Pedro André
13	Ratio	Ratio		Mendes, Pedro André	relative percentage of the sum of beta-alanin and gama-aminobutiric acid
14	Aspartic acid/beta-Alanine ratio	Asp/b-Ala		Mendes, Pedro André
15	Glutamic acid/gamma-Aminobutyric acid ratio	Glu/g-Aba		Mendes, Pedro André
16	Dauwe index	Dauwe		Mendes, Pedro André
17	Aspartic acid	Asp	µmol/g	Mendes, Pedro André
18	Glutamic acid	Glu	µmol/g	Mendes, Pedro André
19	Serine	Ser	µmol/g	Mendes, Pedro André
20	Histidine	His	µmol/g	Mendes, Pedro André
21	Glycine	Gly	µmol/g	Mendes, Pedro André
22	Threonine	Thr	µmol/g	Mendes, Pedro André
23	Arginine	Arg	µmol/g	Mendes, Pedro André
24	Alanine	Ala	µmol/g	Mendes, Pedro André
25	Tyrosine	Tyr	µmol/g	Mendes, Pedro André
26	Methionine	Met	µmol/g	Mendes, Pedro André
27	Valine	Val	µmol/g	Mendes, Pedro André
28	Phenylalanine	Phe	µmol/g	Mendes, Pedro André
29	Isoleucine	Ile	µmol/g	Mendes, Pedro André
30	Leucine	Leu	µmol/g	Mendes, Pedro André
31	Lysine	Lys	µmol/g	Mendes, Pedro André
32	beta-Alanine	b-ala	µmol/g	Mendes, Pedro André
33	gamma-Aminobutyric acid	g-ABA	µmol/g	Mendes, Pedro André
34	Amino acids	AA	µmol/g	Mendes, Pedro André	alpha-Aminobutyric acid
35	Ornithine	Orn	µmol/g	Mendes, Pedro André
36	Amino acid, total hydrolysable	THAA	µmol/g	Mendes, Pedro André

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Size:

552 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Exp treat	2 Time [h]	3 pH	4 Part conc [mg/l]	5 TN [%]	6 TC [%]	7 POC [%]	8 POC [mg/l]	9 PIC [%]	10 C/N	11 Size [µm]	12 Sinking V [m/day]	13 Ratio	14 Asp/b-Ala	15 Glu/g-Aba	16 Dauwe	17 Asp [µmol/g]	18 Glu [µmol/g]	19 Ser [µmol/g]	20 His [µmol/g]	21 Gly [µmol/g]	22 Thr [µmol/g]	23 Arg [µmol/g]	24 Ala [µmol/g]	25 Tyr [µmol/g]	26 Met [µmol/g]	27 Val [µmol/g]	28 Phe [µmol/g]	29 Ile [µmol/g]	30 Leu [µmol/g]	31 Lys [µmol/g]	32 b-ala [µmol/g]	33 g-ABA [µmol/g]	34 AA [µmol/g]	35 Orn [µmol/g]	36 THAA [µmol/g]
ambient	0									104	116
ambient	2	7.85	43.40	0.73	6.60	5.61	2.43	1.00	7.67			0.182	59.262		0.710	55.961	59.499	35.642	10.956	62.001	48.654	17.882	62.599	7.599		31.321	23.443	25.699	45.979	28.224	0.944			1.387	517.789
ambient	6	7.58	50.80	5.59	25.25	18.89	9.60	6.36	3.38			2.430	48.634	4.780	1.450	111.659	143.233	78.105	39.799	136.571	115.250	45.664	158.395	35.565	0.000	77.498	55.123	72.406	123.189	98.936	2.296	29.965	1.464	2.645	1327.762
ambient	9									75	64
ambient	11									63	31
ambient	13	7.49	45.20	4.80	28.67	15.91	7.19	12.76	3.31			0.119	75.975		1.768	163.584	200.432	99.312	66.681	174.213	150.802	66.855	218.846	55.284	9.936	102.063	78.409	100.575	167.619	139.743	2.153		1.450	4.241	1802.199
ambient	16	7.45	37.00	6.47	28.68	27.58	10.20	1.10	4.27			0.136	77.713		1.490	205.973	238.105	98.985	92.119	196.694	157.037	72.559	222.212	16.371		121.490	81.875	107.546	175.782	157.357	2.650		1.517	4.657	1952.931
ambient	18	7.65	36.80	8.26	37.59	28.92	10.64	8.67	3.50			0.864	68.978	13.632	1.596	127.506	160.692	97.068	27.831	142.243	141.816	68.393	189.218	52.032	13.509	80.277	79.072	87.314	157.691	136.238	1.848	11.788	1.748	2.914	1579.198
ambient	20	7.60	30.13	7.32	33.30	32.18	9.70	1.13	4.40			0.622	73.178	18.102	2.018	136.689	169.601	108.417	73.895	150.369	169.036	96.795	203.833	59.194	19.138	86.757	91.791	96.060	172.687	156.858	1.868	9.369	2.233	2.836	1807.425
ambient	23	7.22	21.28	11.12	45.08	42.32	9.01	2.75	3.81			0.087	79.825		2.684	135.154	168.515	111.802	122.581	146.698	188.310	122.841	202.879	68.403	49.352	85.775	110.955	97.703	176.826	159.919	1.693		2.696	3.338	1955.442
high	0									104	116
high	2	7.77	60.00	3.44	18.09	12.64	7.58	5.45	3.67	75	72	3.032	61.368	3.710	1.001	85.258	100.208	64.494	21.053	107.215	87.881	31.183	109.329	21.457	0.000	56.325	41.456	47.197	83.711	48.658	1.389	27.013	0.849	1.955	936.631
high	6	7.77	70.00	7.05	35.65	28.07	19.65	7.57	3.98			5.188	54.264	1.759	1.631	94.802	125.984	94.490	36.231	131.159	124.155	49.434	171.126	37.398	2.006	75.314	62.879	79.630	140.064	109.474	1.747	71.604	1.961	4.344	1413.802
high	9	7.24	57.40	4.81	24.50	23.40	13.43	1.10	4.87			2.388	41.339	2.788	4.568	84.225	114.465	85.717	33.208	120.130	120.091	47.917	159.574	32.522	0.126	70.810	584.497	74.973	131.240	97.161	2.037	41.063	1.294	3.836	1804.889
high	11	7.07	59.00	3.52	17.33	5.84	3.45	11.49	1.66			3.189	55.061	3.252	1.337	107.824	143.112	93.519	33.590	147.832	126.131	51.339	176.544	34.251	0.000	82.675	60.054	79.915	139.970	112.860	1.958	44.012	1.337	4.756	1441.681
high	13	7.24	61.20	4.59	26.02	22.65	13.86	3.37	4.94	75	64	2.682	49.095	4.300	1.181	92.708	121.018	68.107	25.910	111.768	94.802	41.921	132.100	25.125	0.000	60.698	43.684	57.176	100.832	110.138	1.888	28.146		3.922	1119.943
high	16	7.25	86.07	12.25	61.05	13.51	11.63		1.10			3.028	55.734	3.127	1.614	51.892	67.515	49.066	19.047	63.488	73.478	27.514	84.422	16.750	4.664	37.475	37.883	40.640	72.388	70.058	0.931	21.594	1.266	3.937	744.007
high	18	7.19	23.33	6.46	32.81	27.13	6.33	5.68	4.20			2.270	46.481	4.932	1.227	96.521	125.050	83.215	26.304	116.985	111.154	41.099	151.716	25.378	0.000	63.043	53.511	64.211	118.021	98.725	2.077	25.357	1.746	4.276	1208.389
high	20	7.20	24.40	5.62	27.42	27.32	6.67	0.10	4.86	104	97	2.381	45.132	5.551	0.956	127.314	170.387	89.318	9.095	156.456	115.185	60.350	183.389	32.743	4.985	91.419	62.184	80.120	141.920	45.523	2.821	30.696	2.127	1.441	1407.475
high	23		14.68	4.50	22.53	20.84	3.06	1.69	4.63			2.323	53.397	4.942	0.975	104.218	136.153	76.477	8.321	132.750	107.461	49.217	157.871	29.831	3.043	76.504	52.881	71.402	123.196	106.288	1.952	27.552	1.710	3.254	1270.085