Citation:

ece3.1382

Name: Saturating light and not increased carbon dioxide under ocean acidification drives photosynthesis and growth in Ulva rigida (Chlorophyta)
Published: 2015-05-27
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Benthos; Bottles or small containers/Aquaria (<20 L); Chlorophyta; Coast and continental shelf; Growth/Morphology; Laboratory experiment; Light; Macroalgae; Other metabolic rates; Plantae; Primary production/Photosynthesis; Single species; South Pacific; Temperate; Ulva rigida

Always quote citation above when using data! You can download the citation in several formats below.

RIS Citation BibTeX Citation Text Citation Facebook TwitterShow Map Google Earth

Abstract:

Carbon physiology of a genetically identified Ulva rigida was investigated under different CO2(aq) and light levels. The study was designed to answer whether (1) light or exogenous inorganic carbon (Ci) pool is driving growth; and (2) elevated CO2(aq) concentration under ocean acidification (OA) will downregulate CAext-mediated inline image dehydration and alter the stable carbon isotope (delta13C) signatures toward more CO2 use to support higher growth rate. At pHT 9.0 where CO2(aq) is <1 ?mol/L, inhibition of the known inline image use mechanisms, that is, direct inline image uptake through the AE port and CAext-mediated inline image dehydration decreased net photosynthesis (NPS) by only 56-83%, leaving the carbon uptake mechanism for the remaining 17-44% of the NPS unaccounted. An in silico search for carbon-concentrating mechanism elements in expressed sequence tag libraries of Ulva found putative light-dependent inline image transporters to which the remaining NPS can be attributed. The shift in delta13C signatures from -22 per mil toward -10 per mil under saturating light but not under elevated CO2(aq) suggest preference and substantial inline image use to support photosynthesis and growth. U. rigida is Ci saturated, and growth was primarily controlled by light. Therefore, increased levels of CO2(aq) predicted for the future will not, in isolation, stimulate Ulva blooms.

Keyword(s):

Benthos; Bottles or small containers/Aquaria (<20 L); Chlorophyta; Coast and continental shelf; Growth/Morphology; Laboratory experiment; Light; Macroalgae; Other metabolic rates; Plantae; Primary production/Photosynthesis; Single species; South Pacific; Temperate; Ulva rigida

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.6. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: -45.800000 * Longitude: 170.700000

Date/Time Start: 2011-10-05T00:00:00 * Date/Time End: 2011-10-05T00:00:00

Event(s):

Otago_Harbour * Latitude: -45.800000 * Longitude: 170.700000 * Date/Time: 2011-10-05T00:00:00 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2015-05-21.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Species	Species		Roleda, Michael Y
2	Figure	Fig		Roleda, Michael Y
3	Treatment	Treat		Roleda, Michael Y		LL=limiting light, SL=saturating light, HC=high CO2/low pH, LC=low CO2/high pH
4	Replicate	Repl		Roleda, Michael Y
5	Inhibition of net photosynthesis	Inhib NP	%	Roleda, Michael Y
6	Growth rate	µ	%/day	Roleda, Michael Y
7	Carbonic anhydrase activity	CA activity	REA/g	Roleda, Michael Y		external
8	Carbonic anhydrase activity	CA activity	REA/g	Roleda, Michael Y		internal
9	δ13C	δ13C	‰ PDB	Roleda, Michael Y
10	Salinity	Sal		Roleda, Michael Y
11	Temperature, water	Temp	°C	Roleda, Michael Y
12	Temperature, water, standard deviation	Temp std dev	±	Roleda, Michael Y
13	Alkalinity, total	AT	µmol/kg	Roleda, Michael Y	Potentiometric titration
14	Alkalinity, total, standard deviation	AT std dev	±	Roleda, Michael Y	Potentiometric titration
15	Carbon, inorganic, dissolved	DIC	µmol/kg	Roleda, Michael Y	Calculated using SWCO2 (Hunter, 2007)
16	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Roleda, Michael Y	Calculated using SWCO2 (Hunter, 2007)
17	Carbon dioxide	CO2	µmol/kg	Roleda, Michael Y	Calculated using SWCO2 (Hunter, 2007)
18	Carbon dioxide, standard deviation	CO2 std dev	±	Roleda, Michael Y	Calculated using SWCO2 (Hunter, 2007)
19	Bicarbonate ion	[HCO3]-	µmol/kg	Roleda, Michael Y	Calculated using SWCO2 (Hunter, 2007)
20	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Roleda, Michael Y	Calculated using SWCO2 (Hunter, 2007)
21	Carbonate ion	[CO3]2-	µmol/kg	Roleda, Michael Y	Calculated using SWCO2 (Hunter, 2007)
22	Carbonate ion, standard deviation	[CO3]2- std dev	±	Roleda, Michael Y	Calculated using SWCO2 (Hunter, 2007)
23	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Roleda, Michael Y	Calculated using SWCO2 (Hunter, 2007)
24	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Roleda, Michael Y	Calculated using SWCO2 (Hunter, 2007)
25	pH	pH		Roleda, Michael Y	Potentiometric	total scale
26	pH, standard deviation	pH std dev	±	Roleda, Michael Y	Potentiometric	total scale
27	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
28	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
29	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
30	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
31	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
32	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
33	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
34	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
35	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

2344 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Species	2 Fig	3 Treat (LL=limiting light, SL=saturat...)	4 Repl	5 Inhib NP [%]	6 µ [%/day]	7 CA activity [REA/g] (external)	8 CA activity [REA/g] (internal)	9 δ13C [‰ PDB]	10 Sal	11 Temp [°C]	12 Temp std dev [±]	13 AT [µmol/kg] (Potentiometric titration)	14 AT std dev [±] (Potentiometric titration)	15 DIC [µmol/kg] (Calculated using SWCO2 (Hunte...)	16 DIC std dev [±] (Calculated using SWCO2 (Hunte...)	17 CO2 [µmol/kg] (Calculated using SWCO2 (Hunte...)	18 CO2 std dev [±] (Calculated using SWCO2 (Hunte...)	19 [HCO3]- [µmol/kg] (Calculated using SWCO2 (Hunte...)	20 [HCO3]- std dev [±] (Calculated using SWCO2 (Hunte...)	21 [CO3]2- [µmol/kg] (Calculated using SWCO2 (Hunte...)	22 [CO3]2- std dev [±] (Calculated using SWCO2 (Hunte...)	23 pCO2water_SST_wet [µatm] (Calculated using SWCO2 (Hunte...)	24 pCO2 std dev [±] (Calculated using SWCO2 (Hunte...)	25 pH (total scale, Potentiometric)	26 pH std dev [±] (total scale, Potentiometric)	27 CSC flag (Calculated using seacarb afte...)	28 CO2 [µmol/kg] (Calculated using seacarb afte...)	29 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	30 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	31 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	32 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	33 DIC [µmol/kg] (Calculated using seacarb afte...)	34 Omega Arg (Calculated using seacarb afte...)	35 Omega Cal (Calculated using seacarb afte...)
Ulva rigida (macroalga)	2	1st DIDS	1	33.33
Ulva rigida (macroalga)	2	1st DIDS	2	53.85
Ulva rigida (macroalga)	2	1st DIDS	3	45.71
Ulva rigida (macroalga)	2	2nd AZ	1	45.83
Ulva rigida (macroalga)	2	2nd AZ	2	35.00
Ulva rigida (macroalga)	2	2nd AZ	3	36.00
Ulva rigida (macroalga)	2	Total DIDS+AZ	1	79.17
Ulva rigida (macroalga)	2	Total DIDS+AZ	2	88.85
Ulva rigida (macroalga)	2	Total DIDS+AZ	3	81.71
Ulva rigida (macroalga)	2	1st AZ	1	37.14
Ulva rigida (macroalga)	2	1st AZ	2	32.00
Ulva rigida (macroalga)	2	1st AZ	3	18.18
Ulva rigida (macroalga)	2	2nd DIDS	1	30.43
Ulva rigida (macroalga)	2	2nd DIDS	2	23.81
Ulva rigida (macroalga)	2	2nd DIDS	3	25.00
Ulva rigida (macroalga)	2	Total AZ+DIDS	1	67.58
Ulva rigida (macroalga)	2	Total AZ+DIDS	2	55.81
Ulva rigida (macroalga)	2	Total AZ+DIDS	3	43.18
Ulva rigida (macroalga)	3	LL/LC	1		8.7				34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	3	LL/LC	2		7.6				34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	3	LL/LC	3		6.0				34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	3	LL/LC	4		13.6				34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	3	LL/LC	5		8.5				34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	3	LL/LC	6		6.5				34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	3	LL/HC	1		9.1				34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30
Ulva rigida (macroalga)	3	LL/HC	2		8.9				34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30
Ulva rigida (macroalga)	3	LL/HC	3		16.0				34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30
Ulva rigida (macroalga)	3	LL/HC	4		5.9				34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30
Ulva rigida (macroalga)	3	LL/HC	5		6.8				34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30
Ulva rigida (macroalga)	3	LL/HC	6		7.7				34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30
Ulva rigida (macroalga)	3	SL/LC	1		19.5				34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	3	SL/LC	2		15.4				34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	3	SL/LC	3		21.4				34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	3	SL/LC	4		20.9				34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	3	SL/LC	5		20.9				34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	3	SL/LC	6		17.5				34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	3	SL/HC	1		24.5				34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30
Ulva rigida (macroalga)	3	SL/HC	2		22.0				34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30
Ulva rigida (macroalga)	3	SL/HC	3		21.0				34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30
Ulva rigida (macroalga)	3	SL/HC	4		22.3				34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30
Ulva rigida (macroalga)	3	SL/HC	5		21.2				34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30
Ulva rigida (macroalga)	3	SL/HC	6		17.3				34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30
Ulva rigida (macroalga)	4	LL/LC	1			3.974	3.974		34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	4	LL/LC	2			1.720	3.408		34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	4	LL/LC	3			6.080	6.775		34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	4	LL/LC	4			4.046	6.706		34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	4	LL/LC	5			5.468	7.278		34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	4	LL/LC	6			0.337	8.724		34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	4	LL/HC	1			4.450	4.450		34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30
Ulva rigida (macroalga)	4	LL/HC	2			4.360	4.360		34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30
Ulva rigida (macroalga)	4	LL/HC	3			0.700	2.716		34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30
Ulva rigida (macroalga)	4	LL/HC	4			4.653	6.627		34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30
Ulva rigida (macroalga)	4	LL/HC	5			5.672	8.938		34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30
Ulva rigida (macroalga)	4	LL/HC	6			2.537	5.262		34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30
Ulva rigida (macroalga)	4	SL/LC	1			1.817	6.060		34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	4	SL/LC	2			2.341	7.811		34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	4	SL/LC	3			6.260	9.253		34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	4	SL/LC	4			5.639	7.632		34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	4	SL/LC	5			1.553	10.755		34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	4	SL/LC	6			4.452	9.996		34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	4	SL/HC	1			3.986	6.450		34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30
Ulva rigida (macroalga)	4	SL/HC	2			4.204	8.342		34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30
Ulva rigida (macroalga)	4	SL/HC	3			4.038	11.942		34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30
Ulva rigida (macroalga)	4	SL/HC	4			5.666	13.689		34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30
Ulva rigida (macroalga)	4	SL/HC	5			5.164	10.035		34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30
Ulva rigida (macroalga)	4	SL/HC	6			2.613	6.376		34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30
Ulva rigida (macroalga)	5	LL/LC	1					-23.665	34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	5	LL/LC	2					-22.050	34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	5	LL/LC	3					-19.813	34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	5	LL/LC	4					-22.271	34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	5	LL/LC	5					-22.991	34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	5	LL/LC	6					-21.939	34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	5	LL/HC	1					-22.558	34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30
Ulva rigida (macroalga)	5	LL/HC	2					-22.756	34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30
Ulva rigida (macroalga)	5	LL/HC	3					-24.062	34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30
Ulva rigida (macroalga)	5	LL/HC	4					-19.843	34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30
Ulva rigida (macroalga)	5	LL/HC	5					-22.365	34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30
Ulva rigida (macroalga)	5	LL/HC	6						34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30
Ulva rigida (macroalga)	5	SL/LC	1					-7.999	34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	5	SL/LC	2					-9.467	34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	5	SL/LC	3					-13.200	34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	5	SL/LC	4					-10.505	34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	5	SL/LC	5					-7.635	34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	5	SL/LC	6						34	13	1	2197.44	11.99	2031.41	11.23	18.49	0.11	1890.43	10.37	122.48	0.88	471.38	2.87	7.965	0.002	8	18.99	474.08	475.84	1902.48	117.96	2039.43	1.81	2.83
Ulva rigida (macroalga)	5	SL/HC	1					-10.110	34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30
Ulva rigida (macroalga)	5	SL/HC	2					-9.147	34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30
Ulva rigida (macroalga)	5	SL/HC	3					-9.277	34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30
Ulva rigida (macroalga)	5	SL/HC	4					-9.772	34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30
Ulva rigida (macroalga)	5	SL/HC	5					-11.605	34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30
Ulva rigida (macroalga)	5	SL/HC	6					-8.763	34	13	1	2207.55	15.97	2170.76	15.74	48.02	0.51	2066.36	14.97	56.38	0.66	1223.91	13.02	7.590	0.004	8	49.06	1224.48	1229.04	2072.16	54.18	2175.40	0.83	1.30