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Gutowska, Magdalena A; Melzner, Frank; Pörtner, Hans-Otto; Meier, Sebastian (2015): Cuttlebone morphometry measurements. PANGAEA,, Supplement to: Gutowska, MA et al. (2010): Cuttlebone calcification increases during exposure to elevated seawater pCO2 in the cephalopod Sepia officinalis. Marine Biology, 157(7), 1653-1663,

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Changes in seawater carbonate chemistry that accompany ongoing ocean acidification have been found to affect calcification processes in many marine invertebrates. In contrast to the response of most invertebrates, calcification rates increase in the cephalopod Sepia officials during long-term exposure to elevated seawater pCO2. The present trial investigated structural changes in the cuttlebones of S. officinalis calcified during 6 weeks of exposure to 615 Pa CO2. Cuttlebone mass increased sevenfold over the course of the growth trail, reaching a mean value of 0.71 ± 0.15 g. Depending on cuttlefish size (mantle lengths 44-56 mm), cuttlebones of CO2-incubated individuals accreted 22-55% more CaCO3 compared to controls at 64 Pa CO2. However, the height of the CO2- exposed cuttlebones was reduced. A decrease in spacing of the cuttlebone lamellae, from 384 ± 26 to 195 ± 38 lm, accounted for the height reduction The greater CaCO3 content of the CO2-incubated cuttlebones can be attributed to an increase in thickness of the lamellar and pillar walls. Particularly, pillar thickness increased from 2.6 ± 0.6 to 4.9 ± 2.2 lm. Interestingly, the incorporation of non-acidsoluble organic matrix (chitin) in the cuttlebones of CO2- exposed individuals was reduced by 30% on average. The apparent robustness of calcification processes in S. officials, and other powerful ion regulators such as decapod cructaceans, during exposure to elevated pCO2 is predicated to be closely connected to the increased extracellular [HCO3 -] maintained by these organisms to compensate extracellular pH. The potential negative impact of increased calcification in the cuttlebone of S. officials is discussed with regard to its function as a lightweight and highly porous buoyancy regulation device. Further studies working with lower seawater pCO2 values are necessary to evaluate if the observed phenomenon is of ecological relevance.
Further details:
Lavigne, Héloise; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0.
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2015-04-27.
#NameShort NameUnitPrincipal InvestigatorMethodComment
1SpeciesSpeciesGutowska, Magdalena A
2FigureFigGutowska, Magdalena A
3Experimental treatmentExp treatGutowska, Magdalena A
4LengthlmmGutowska, Magdalena A
5WidthwmmGutowska, Magdalena A
6MassMassgGutowska, Magdalena A
7HeighthmmGutowska, Magdalena A
8Calcium carbonate, massCaCO3gGutowska, Magdalena Acuttlebone
9MassMassgGutowska, Magdalena ANASOM
10Temperature, waterTemp°CGutowska, Magdalena A
11Temperature, water, standard deviationTemp std dev±Gutowska, Magdalena A
12SalinitySalGutowska, Magdalena A
13Salinity, standard deviationSal std dev±Gutowska, Magdalena A
14pHpHGutowska, Magdalena ANBS scale
15pH, standard deviationpH std dev±Gutowska, Magdalena ANBS scale
16Carbon, inorganic, dissolvedDICµmol/kgGutowska, Magdalena A
17Carbon, inorganic, dissolved, standard deviationDIC std dev±Gutowska, Magdalena A
18Carbon dioxide, partial pressurepCO2PaGutowska, Magdalena A
19Partial pressure of carbon dioxide, standard deviationpCO2 std dev±Gutowska, Magdalena A
20Aragonite saturation stateOmega ArgGutowska, Magdalena A
21Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
22pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
23Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
24Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
25Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
26Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
27Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
28Alkalinity, totalATµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
29Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
30Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
1680 data points

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