Citation:

fmicb.2013.00155

Name: The effect of nitrate and phosphate availability on Emiliania huxleyi(NZEH) physiology under different CO2 scenarios
Published: 2013
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Macro-nutrients; Other metabolic rates; Pelagos; Phytoplankton; Single species; South Pacific

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Abstract:

Growth and calcification of the marine coccolithophorid Emiliania huxleyi is affected by ocean acidification and macronutrients limitation and its response varies between strains. Here we investigated the physiological performance of a highly calcified E. huxleyi strain, NZEH, in a multiparametric experiment. Cells were exposed to different CO2 levels (ranging from 250 to 1314 µatm) under three nutrient conditions [nutrient replete (R), nitrate limited (-N), and phosphate limited (-P)]. We focused on calcite and organic carbon quotas and on nitrate and phosphate utilization by analyzing the activity of nitrate reductase (NRase) and alkaline phosphatase (APase), respectively. Particulate inorganic (PIC) and organic (POC) carbon quotas increased with increasing CO2 under R conditions but a different pattern was observed under nutrient limitation. The PIC:POC ratio decreased with increasing CO2 in nutrient limited cultures. Coccolith length increased with CO2 under all nutrient conditions but the coccosphere volume varied depending on the nutrient treatment. Maximum APase activity was found at 561 matm of CO2 (pH 7.92) in -P cultures and in R conditions, NRase activity increased linearly with CO2. These results suggest that E. huxleyi's competitive ability for nutrient uptake might be altered in future high-CO2 oceans. The combined dataset will be useful in model parameterizations of the carbon cycle and ocean acidification.

Keyword(s):

Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Macro-nutrients; Other metabolic rates; Pelagos; Phytoplankton; Single species; South Pacific

Further details:

Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2015-01-29.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Species	Species		Rouco, Mónica
2	Table	Tab		Rouco, Mónica
3	Figure	Fig		Rouco, Mónica
4	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Rouco, Mónica		treatment
5	Treatment	Treat		Rouco, Mónica
6	Nitrate reductase activity, per total protein	NO3 reduct act	UEA/#	Rouco, Mónica
7	Alkaline phosphatase, para-Nitrophenylphosphate per cell	ALKP pNPP/cell	nmol/min/#	Rouco, Mónica
8	Particulate organic carbon, per cell	POC/cell	pmol/#	Rouco, Mónica
9	Particulate organic carbon content per cell, standard deviation	POC cont/cell std dev	±	Rouco, Mónica
10	Particulate organic nitrogen per cell	PON/cell	pmol/#	Rouco, Mónica
11	Particulate organic nitrogen per cell, standard deviation	PON/cell std dev	±	Rouco, Mónica
12	Particulate inorganic carbon per cell	PIC/cell	pmol/#	Rouco, Mónica
13	Particulate inorganic carbon per cell, standard deviation	PIC/cell std dev	±	Rouco, Mónica
14	Particulate organic phosphorus per cell	POP/cell	pmol/#	Rouco, Mónica
15	Particulate organic phosphorus per cell, standard deviation	POP/cell std dev	±	Rouco, Mónica
16	Particulate inorganic carbon/particulate organic carbon ratio	PIC/POC		Rouco, Mónica
17	Particulate inorganic carbon/particulate organic carbon ratio, standard deviation	PIC/POC ratio std dev	±	Rouco, Mónica
18	Coccoliths, volume	Cocco vol	µm³	Rouco, Mónica
19	Coccoliths, volume, standard deviation	Cocco vol std dev	±	Rouco, Mónica
20	Coccosphere, length	Coccosp l	µm	Rouco, Mónica
21	Coccosphere, length, standard deviation	Coccosp l std dev	±	Rouco, Mónica
22	Temperature, water	Temp	°C	Rouco, Mónica
23	Temperature, water, standard deviation	Temp std dev	±	Rouco, Mónica
24	Salinity	Sal		Rouco, Mónica
25	Trientalis-type	Tre-T	#	Rouco, Mónica
26	Irradiance	E	µmol/m²/s	Rouco, Mónica
27	Irradiance, standard deviation	E std dev	±	Rouco, Mónica
28	Nitrate	NITRATE	µmol/kg	Rouco, Mónica		at the end of the experiment
29	Nitrate, standard deviation	NO3 std dev	±	Rouco, Mónica		at the end of the experiment
30	Phosphate	PHSPHT	µmol/kg	Rouco, Mónica		at the end of the experiment
31	Phosphate, standard deviation	[PO4]3- std dev	±	Rouco, Mónica		at the end of the experiment
32	Carbon, inorganic, dissolved	DIC	µmol/kg	Rouco, Mónica	Coulometric titration	at the end of the experiment
33	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Rouco, Mónica	Coulometric titration	at the end of the experiment
34	Alkalinity, total	AT	µmol/kg	Rouco, Mónica	Potentiometric titration	at the end of the experiment
35	Alkalinity, total, standard deviation	AT std dev	±	Rouco, Mónica	Potentiometric titration	at the end of the experiment
36	pH	pH		Rouco, Mónica	Calculated using CO2SYS	total scale, at the end of the experiment
37	pH, standard deviation	pH std dev	±	Rouco, Mónica	Calculated using CO2SYS	total scale, at the end of the experiment
38	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Rouco, Mónica	Calculated using CO2SYS	at the end of the experiment
39	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Rouco, Mónica	Calculated using CO2SYS	at the end of the experiment
40	Bicarbonate ion	[HCO3]-	µmol/kg	Rouco, Mónica	Calculated using CO2SYS	at the end of the experiment
41	Bicarbonate, standard deviation	[HCO3]- std dev	±	Rouco, Mónica	Calculated using CO2SYS	at the end of the experiment
42	Carbonate ion	[CO3]2-	µmol/kg	Rouco, Mónica	Calculated using CO2SYS	at the end of the experiment
43	Carbonate ion, standard deviation	[CO3]2- std dev	±	Rouco, Mónica	Calculated using CO2SYS	at the end of the experiment
44	Carbon dioxide	CO2	µmol/kg	Rouco, Mónica	Calculated using CO2SYS	at the end of the experiment
45	Carbon dioxide, standard deviation	CO2 std dev	±	Rouco, Mónica	Calculated using CO2SYS	at the end of the experiment
46	Calcite saturation state	Omega Cal		Rouco, Mónica	Calculated using CO2SYS	at the end of the experiment
47	Calcite saturation state, standard deviation	Omega Cal std dev	±	Rouco, Mónica	Calculated using CO2SYS	at the end of the experiment
48	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
49	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
50	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
51	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
52	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
53	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
54	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
55	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
56	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1422 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Species	2 Tab	3 Fig	4 pCO2water_SST_wet [µatm] (treatment)	5 Treat	6 NO3 reduct act [UEA/#]	7 ALKP pNPP/cell [nmol/min/#]	8 POC/cell [pmol/#]	9 POC cont/cell std dev [±]	10 PON/cell [pmol/#]	11 PON/cell std dev [±]	12 PIC/cell [pmol/#]	13 PIC/cell std dev [±]	14 POP/cell [pmol/#]	15 POP/cell std dev [±]	16 PIC/POC	17 PIC/POC ratio std dev [±]	18 Cocco vol [µm³]	19 Cocco vol std dev [±]	20 Coccosp l [µm]	21 Coccosp l std dev [±]	22 Temp [°C]	23 Temp std dev [±]	24 Sal	25 Tre-T [#]	26 E [µmol/m²/s]	27 E std dev [±]	28 NITRATE [µmol/kg] (at the end of the experiment)	29 NO3 std dev [±] (at the end of the experiment)	30 PHSPHT [µmol/kg] (at the end of the experiment)	31 [PO4]3- std dev [±] (at the end of the experiment)	32 DIC [µmol/kg] (at the end of the experiment,...)	33 DIC std dev [±] (at the end of the experiment,...)	34 AT [µmol/kg] (at the end of the experiment,...)	35 AT std dev [±] (at the end of the experiment,...)	36 pH (total scale, at the end of th...)	37 pH std dev [±] (total scale, at the end of th...)	38 pCO2water_SST_wet [µatm] (at the end of the experiment,...)	39 pCO2 std dev [±] (at the end of the experiment,...)	40 [HCO3]- [µmol/kg] (at the end of the experiment,...)	41 [HCO3]- std dev [±] (at the end of the experiment,...)	42 [CO3]2- [µmol/kg] (at the end of the experiment,...)	43 [CO3]2- std dev [±] (at the end of the experiment,...)	44 CO2 [µmol/kg] (at the end of the experiment,...)	45 CO2 std dev [±] (at the end of the experiment,...)	46 Omega Cal (at the end of the experiment,...)	47 Omega Cal std dev [±] (at the end of the experiment,...)	48 CSC flag (Calculated using seacarb afte...)	49 pH (total scale, Calculated using...)	50 CO2 [µmol/kg] (Calculated using seacarb afte...)	51 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	52 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	53 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	54 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	55 Omega Arg (Calculated using seacarb afte...)	56 Omega Cal (Calculated using seacarb afte...)
Emiliania huxleyi (phytoplankton)		1	258	nutrient-replete	0.000026498																19	0.5	34	0.4	120	15	149.0	10.40	3.0	0.1	1808	5	2141	4.0	8.21	0.00	225	2	1575	5	225.0	1.0	8	0	5.4	0.0	15	8.23	7.49	223.52	224.30	1575.69	224.82	3.50	5.41
Emiliania huxleyi (phytoplankton)		1	258	nutrient-replete	0.000022861																19	0.5	34	0.4	120	15	149.0	10.40	3.0	0.1	1808	5	2141	4.0	8.21	0.00	225	2	1575	5	225.0	1.0	8	0	5.4	0.0	15	8.23	7.49	223.52	224.30	1575.69	224.82	3.50	5.41
Emiliania huxleyi (phytoplankton)		1	258	nutrient-replete	0.000021444																19	0.5	34	0.4	120	15	149.0	10.40	3.0	0.1	1808	5	2141	4.0	8.21	0.00	225	2	1575	5	225.0	1.0	8	0	5.4	0.0	15	8.23	7.49	223.52	224.30	1575.69	224.82	3.50	5.41
Emiliania huxleyi (phytoplankton)		1	555	nutrient-replete	0.000026294																19	0.5	34	0.4	120	15	147.6	0.90	2.6	0.0	1937	65	2113	7.0	7.91	0.01	519	9	1793	5	127.0	2.0	17	0	3.0	0.0	15	7.92	17.20	513.41	515.18	1793.05	126.75	1.97	3.05
Emiliania huxleyi (phytoplankton)		1	555	nutrient-replete	0.000027614																19	0.5	34	0.4	120	15	147.6	0.90	2.6	0.0	1937	65	2113	7.0	7.91	0.01	519	9	1793	5	127.0	2.0	17	0	3.0	0.0	15	7.92	17.20	513.41	515.18	1793.05	126.75	1.97	3.05
Emiliania huxleyi (phytoplankton)		1	555	nutrient-replete	0.000029825																19	0.5	34	0.4	120	15	147.6	0.90	2.6	0.0	1937	65	2113	7.0	7.91	0.01	519	9	1793	5	127.0	2.0	17	0	3.0	0.0	15	7.92	17.20	513.41	515.18	1793.05	126.75	1.97	3.05
Emiliania huxleyi (phytoplankton)		1	1073	nutrient-replete	0.000032786																19	0.5	34	0.4	120	15	149.4	0.90	1.7	0.1	2102	14	2179	12.0	7.64	0.01	1080	27	1990	14	75.0	1.0	36	1	1.8	0.0	15	7.65	35.91	1071.79	1075.49	1991.21	74.88	1.17	1.80
Emiliania huxleyi (phytoplankton)		1	1073	nutrient-replete	0.000045675																19	0.5	34	0.4	120	15	149.4	0.90	1.7	0.1	2102	14	2179	12.0	7.64	0.01	1080	27	1990	14	75.0	1.0	36	1	1.8	0.0	15	7.65	35.91	1071.79	1075.49	1991.21	74.88	1.17	1.80
Emiliania huxleyi (phytoplankton)		1	1073	nutrient-replete	0.000040630																19	0.5	34	0.4	120	15	149.4	0.90	1.7	0.1	2102	14	2179	12.0	7.64	0.01	1080	27	1990	14	75.0	1.0	36	1	1.8	0.0	15	7.65	35.91	1071.79	1075.49	1991.21	74.88	1.17	1.80
Emiliania huxleyi (phytoplankton)		1	258	phosphate-limited		0.0000016273															19	0.5	34	0.4	120	15	156.7	0.21	0.0	0.0	1790	7	2097	8.0	8.18	0.00	243	3	1573	7	209.0	2.0	8	0	5.0	0.0	15	8.20	8.04	239.88	240.70	1573.14	208.82	3.25	5.03
Emiliania huxleyi (phytoplankton)		1	258	phosphate-limited		0.0000014450															19	0.5	34	0.4	120	15	156.7	0.21	0.0	0.0	1790	7	2097	8.0	8.18	0.00	243	3	1573	7	209.0	2.0	8	0	5.0	0.0	15	8.20	8.04	239.88	240.70	1573.14	208.82	3.25	5.03
Emiliania huxleyi (phytoplankton)		1	258	phosphate-limited		0.0000012087															19	0.5	34	0.4	120	15	156.7	0.21	0.0	0.0	1790	7	2097	8.0	8.18	0.00	243	3	1573	7	209.0	2.0	8	0	5.0	0.0	15	8.20	8.04	239.88	240.70	1573.14	208.82	3.25	5.03
Emiliania huxleyi (phytoplankton)		1	555	phosphate-limited		0.0000068039															19	0.5	34	0.4	120	15	153.3	1.50	0.0	0.0	1890	3	2109	8.0	8.01	0.01	394	14	1723	5	154.0	5.0	13	1	3.7	0.1	15	8.03	13.04	389.16	390.50	1722.62	154.34	2.40	3.72
Emiliania huxleyi (phytoplankton)		1	555	phosphate-limited		0.0000062913															19	0.5	34	0.4	120	15	153.3	1.50	0.0	0.0	1890	3	2109	8.0	8.01	0.01	394	14	1723	5	154.0	5.0	13	1	3.7	0.1	15	8.03	13.04	389.16	390.50	1722.62	154.34	2.40	3.72
Emiliania huxleyi (phytoplankton)		1	555	phosphate-limited		0.0000056754															19	0.5	34	0.4	120	15	153.3	1.50	0.0	0.0	1890	3	2109	8.0	8.01	0.01	394	14	1723	5	154.0	5.0	13	1	3.7	0.1	15	8.03	13.04	389.16	390.50	1722.62	154.34	2.40	3.72
Emiliania huxleyi (phytoplankton)		1	1073	phosphate-limited		0.0000023797															19	0.5	34	0.4	120	15	151.5	0.20	0.0	0.0	2098	3	2153	3.3	7.57	0.00	1253	8	1992	3	64.8	0.4	42	0	1.6	0.0	15	7.59	41.53	1239.27	1243.55	1991.69	64.79	1.01	1.56
Emiliania huxleyi (phytoplankton)		1	1073	phosphate-limited		0.0000022275															19	0.5	34	0.4	120	15	151.5	0.20	0.0	0.0	2098	3	2153	3.3	7.57	0.00	1253	8	1992	3	64.8	0.4	42	0	1.6	0.0	15	7.59	41.53	1239.27	1243.55	1991.69	64.79	1.01	1.56
Emiliania huxleyi (phytoplankton)		1	1073	phosphate-limited		0.0000025785															19	0.5	34	0.4	120	15	151.5	0.20	0.0	0.0	2098	3	2153	3.3	7.57	0.00	1253	8	1992	3	64.8	0.4	42	0	1.6	0.0	15	7.59	41.53	1239.27	1243.55	1991.69	64.79	1.01	1.56
Emiliania huxleyi (phytoplankton)		2		nutrient-replete	0.000026498		0.78		0.11												19	0.5	34	0.4	120	15
Emiliania huxleyi (phytoplankton)		2		nutrient-replete	0.000022861		0.78		0.11												19	0.5	34	0.4	120	15
Emiliania huxleyi (phytoplankton)		2		nutrient-replete	0.000021444		0.69		0.10												19	0.5	34	0.4	120	15
Emiliania huxleyi (phytoplankton)		2		nutrient-replete	0.000026294		0.67		0.09												19	0.5	34	0.4	120	15
Emiliania huxleyi (phytoplankton)		2		nutrient-replete	0.000027614		0.77		0.11												19	0.5	34	0.4	120	15
Emiliania huxleyi (phytoplankton)		2		nutrient-replete	0.000029825		0.78		0.11												19	0.5	34	0.4	120	15
Emiliania huxleyi (phytoplankton)		2		nutrient-replete	0.000032786		1.25		0.16												19	0.5	34	0.4	120	15
Emiliania huxleyi (phytoplankton)		2		nutrient-replete	0.000045675		1.33		0.18												19	0.5	34	0.4	120	15
Emiliania huxleyi (phytoplankton)		2		nutrient-replete	0.000040630		1.70		0.22												19	0.5	34	0.4	120	15
Emiliania huxleyi (phytoplankton)		3		phosphate-limited		0.0000016273	1.25				1.13										19	0.5	34	0.4	120	15
Emiliania huxleyi (phytoplankton)		3		phosphate-limited		0.0000014450	1.35				1.45										19	0.5	34	0.4	120	15
Emiliania huxleyi (phytoplankton)		3		phosphate-limited		0.0000012087	1.43				1.35										19	0.5	34	0.4	120	15
Emiliania huxleyi (phytoplankton)		3		phosphate-limited		0.0000068039	2.05				2.00										19	0.5	34	0.4	120	15
Emiliania huxleyi (phytoplankton)		3		phosphate-limited		0.0000062913	2.00				2.05										19	0.5	34	0.4	120	15
Emiliania huxleyi (phytoplankton)		3		phosphate-limited		0.0000056754	2.23				2.05										19	0.5	34	0.4	120	15
Emiliania huxleyi (phytoplankton)		3		phosphate-limited		0.0000023797	2.02				1.40										19	0.5	34	0.4	120	15
Emiliania huxleyi (phytoplankton)		3		phosphate-limited		0.0000022275	1.97				1.40										19	0.5	34	0.4	120	15
Emiliania huxleyi (phytoplankton)		3		phosphate-limited		0.0000025785	1.97				1.55										19	0.5	34	0.4	120	15
Emiliania huxleyi (phytoplankton)	2	4	258	nutrient-replete			0.75	0.05	0.11	0.01	0.70	0.03	0.0102	0.0006	0.93	0.04	55.06	8.17	2.974	0.463	19	0.5	34	0.4	120	15	149.0	10.40	3.0	0.1	1808	5	2141	4.0	8.21	0.00	225	2	1575	5	225.0	1.0	8	0	5.4	0.0	15	8.23	7.49	223.52	224.30	1575.69	224.82	3.50	5.41
Emiliania huxleyi (phytoplankton)	2	4	555	nutrient-replete			0.74	0.06	0.10	0.01	0.63	0.06	0.0113	0.0019	0.85	0.02	58.68	4.58	2.977	0.429	19	0.5	34	0.4	120	15	147.6	0.90	2.6	0.0	1937	65	2113	7.0	7.91	0.01	519	9	1793	5	127.0	2.0	17	0	3.0	0.0	15	7.92	17.20	513.41	515.18	1793.05	126.75	1.97	3.05
Emiliania huxleyi (phytoplankton)	2	4	1073	nutrient-replete			1.43	0.24	0.19	0.03	1.20	0.08	0.0198	0.0024	0.86	0.11	76.82	2.74	3.292	0.482	19	0.5	34	0.4	120	15	149.4	0.90	1.7	0.1	2102	14	2179	12.0	7.64	0.01	1080	27	1990	14	75.0	1.0	36	1	1.8	0.0	15	7.65	35.91	1071.79	1075.49	1991.21	74.88	1.17	1.80
Emiliania huxleyi (phytoplankton)	2	5	258	nitrate-limited			1.27	0.10	0.06	0.00	1.50	0.07	0.0105	0.0001	1.18	0.05	49.64	2.40	3.042	0.328	19	0.5	34	0.4	120	15	0.3	0.10	2.9	0.4	1734	4	2021	3.0	8.16	0.01	251	5	1534	5	192.0	3.0	8	0	4.6	0.1	15	8.17	8.36	249.62	250.48	1534.66	190.98	2.98	4.60
Emiliania huxleyi (phytoplankton)	2	5	555	nitrate-limited			1.35	0.02	0.06	0.01	1.51	0.08	0.0113	0.0003	1.12	0.04	46.22	3.38	3.055	0.314	19	0.5	34	0.4	120	15	0.1	0.00	2.9	0.0	1917	4	2111	5.0	7.95	0.00	465	4	1765	3	137.0	1.0	16	0	3.3	0.0	15	7.96	15.39	459.26	460.84	1764.41	137.20	2.14	3.30
Emiliania huxleyi (phytoplankton)	2	5	1073	nitrate-limited			1.49	0.09	0.06	0.01	1.54	0.07	0.0141	0.0000	1.04	0.03	49.70	0.33	3.224	0.364	19	0.5	34	0.4	120	15	0.1	0.00	1.6	0.0	1958	30	1994	1.0	7.51	0.01	1358	25	1860	3	52.0	1.0	45	1	1.3	0.0	15	7.52	45.16	1347.63	1352.28	1860.84	52.01	0.81	1.25
Emiliania huxleyi (phytoplankton)	2	6	258	phosphate-limited			1.34	0.09	0.13	0.02	1.31	0.17	0.0039	0.0003	0.97	0.09	87.82	7.04	3.084	0.328	19	0.5	34	0.4	120	15	156.7	0.21	0.0	0.0	1790	7	2097	8.0	8.18	0.00	243	3	1573	7	209.0	2.0	8	0	5.0	0.0	15	8.20	8.04	239.88	240.70	1573.14	208.82	3.25	5.03
Emiliania huxleyi (phytoplankton)	2	6	555	phosphate-limited			2.10	0.12	0.16	0.01	2.03	0.03	0.0043	0.0022	0.97	0.05	118.67	2.03	3.228	0.312	19	0.5	34	0.4	120	15	153.3	1.50	0.0	0.0	1890	3	2109	8.0	8.01	0.01	394	14	1723	5	154.0	5.0	13	1	3.7	0.1	15	8.03	13.04	389.16	390.50	1722.62	154.34	2.40	3.72
Emiliania huxleyi (phytoplankton)	2	6	1073	phosphate-limited			1.99	0.03	0.18	0.01	1.45	0.10	0.0059	0.0008	0.73	0.05	104.46	1.91	3.427	0.403	19	0.5	34	0.4	120	15	151.5	0.20	0.0	0.0	2098	3	2153	3.3	7.57	0.00	1253	8	1992	3	64.8	0.4	42	0	1.6	0.0	15	7.59	41.53	1239.27	1243.55	1991.69	64.79	1.01	1.56