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Fernández, Pamela A; Hurd, Catriona L; Roleda, Michael Y (2014): Bicarbonate uptake via an anion exchange protein is the main mechanism of inorganic carbon acquisition by the giant kelp Macrocystis pyrifera (Laminariales, Phaeophyceae) under variable pH [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.839919, Supplement to: Fernández, PA et al. (2014): Bicarbonate uptake via an anion exchange protein is the main mechanism of inorganic carbon acquisition by the giant kelp Macrocystis pyrifera (Laminariales, Phaeophyceae) under variable pH. Journal of Phycology, 50(6), 998-1008, https://doi.org/10.1111/jpy.12247

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Abstract:
Macrocystis pyrifera is a widely distributed, highly productive, seaweed. It is known to use bicarbonate (HCO3-) from seawater in photosynthesis and the main mechanism of utilization is attributed to the external catalyzed dehydration of HCO3- by the surface-bound enzyme carbonic anhydrase (CAext). Here, we examined other putative HCO3- uptake mechanisms in M. pyrifera under pHT 9.00 (HCO3-: CO2 = 940:1) and pHT 7.65 (HCO3-: CO2 = 51:1). Rates of photosynthesis, and internal CA (CAint) and CAext activity were measured following the application of AZ which inhibits CAext, and DIDS which inhibits a different HCO3- uptake system, via an anion exchange (AE) protein. We found that the main mechanism of HCO3- uptake by M. pyrifera is via an AE protein, regardless of the HCO3-: CO2 ratio, with CAext making little contribution. Inhibiting the AE protein led to a 55%-65% decrease in photosynthetic rates. Inhibiting both the AE protein and CAext at pHT 9.00 led to 80%-100% inhibition of photosynthesis, whereas at pHT 7.65, passive CO2 diffusion supported 33% of photosynthesis. CAint was active at pHT 7.65 and 9.00, and activity was always higher than CAext, because of its role in dehydrating HCO3- to supply CO2 to RuBisCO. Interestingly, the main mechanism of HCO3- uptake in M. pyrifera was different than that in other Laminariales studied (CAext-catalyzed reaction) and we suggest that species-specific knowledge of carbon uptake mechanisms is required in order to elucidate how seaweeds might respond to future changes in HCO3-:CO2 due to ocean acidification.
Keyword(s):
Benthos; Bottles or small containers/Aquaria (<20 L); Chromista; Coast and continental shelf; Laboratory experiment; Macroalgae; Macrocystis pyrifera; Ochrophyta; Other metabolic rates; Primary production/Photosynthesis; Single species; South Pacific; Temperate
Further details:
Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb
Project(s):
Ocean Acidification International Coordination Centre (OA-ICC)
Coverage:
Latitude: -45.783330 * Longitude: 170.716670
Date/Time Start: 2013-02-01T00:00:00 * Date/Time End: 2013-02-28T00:00:00
Event(s):
Aromoana * Latitude: -45.783330 * Longitude: 170.716670 * Date/Time Start: 2013-02-01T00:00:00 * Date/Time End: 2013-02-28T00:00:00 * Method/Device: Experiment (EXP)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-12-02.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1 SpeciesSpeciesFernández, Pamela A
2 pHpHFernández, Pamela Ameasurement, total scale
3 pHpHFernández, Pamela Aacclimated, total scale
4 Net photosynthesis rate, oxygenPN O2µmol/g/hFernández, Pamela A
5 Net photosynthesis rate, oxygen, standard errorPN O2 std e±Fernández, Pamela A
6 Inhibition of net photosynthesisInhib NP%Fernández, Pamela Aeffect of DIDS
7 Inhibition of net photosynthesis, standard errorInhib NP std e±Fernández, Pamela Aeffect of DIDS
8 Inhibition of net photosynthesisInhib NP%Fernández, Pamela Aeffect of AZ
9 Inhibition of net photosynthesis, standard errorInhib NP std e±Fernández, Pamela Aeffect of AZ
10 Carbonic anhydrase activityCA activityREA/gFernández, Pamela Aexternal
11 Carbonic anhydrase activity, standard errorCA act std e±Fernández, Pamela Aexternal
12 Carbonic anhydrase activityCA activityREA/gFernández, Pamela Ainternal
13 Carbonic anhydrase activity, standard errorCA act std e±Fernández, Pamela Ainternal
14 Carbonic anhydrase activityCA activityREA/gFernández, Pamela Ainternal, control
15 Carbonic anhydrase activity, standard errorCA act std e±Fernández, Pamela Ainternal, control
16 Carbonic anhydrase activityCA activityREA/gFernández, Pamela Ainternal, DIDS+AZ
17 Carbonic anhydrase activity, standard errorCA act std e±Fernández, Pamela Ainternal, DIDS+AZ
18 pHpHFernández, Pamela ASpectrophotometrictotal scale
19 Alkalinity, totalATµmol/kgFernández, Pamela APotentiometric titration
20 Carbon, inorganic, dissolvedDICµmol/kgFernández, Pamela ACoulometric titration
21 Temperature, waterTemp°CFernández, Pamela A
22 SalinitySalFernández, Pamela A
23 Bicarbonate ion[HCO3]-µmol/kgFernández, Pamela ACalculated using SWCO2 (Hunter, 2007)
24 Carbon dioxideCO2µmol/kgFernández, Pamela ACalculated using SWCO2 (Hunter, 2007)
25 Carbonate ion[CO3]2-µmol/kgFernández, Pamela ACalculated using SWCO2 (Hunter, 2007)
26 Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmFernández, Pamela ACalculated using SWCO2 (Hunter, 2007)
27 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmFernández, Pamela ACalculated using SWCO2 (Hunter, 2007)
28 Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
29 pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
30 Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
31 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
32 Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
33 Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
34 Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
35 Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
36 Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
465 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 
Species		2 
pH
(measurement, total scale)		3 
pH
(acclimated, total scale)		4 
PN O2 [µmol/g/h]		5 
PN O2 std e [±]		6 
Inhib NP [%]
(effect of DIDS)		7 
Inhib NP std e [±]
(effect of DIDS)		8 
Inhib NP [%]
(effect of AZ)		9 
Inhib NP std e [±]
(effect of AZ)		10 
CA activity [REA/g]
(external)		11 
CA act std e [±]
(external)		12 
CA activity [REA/g]
(internal)		13 
CA act std e [±]
(internal)		14 
CA activity [REA/g]
(internal, control)		15 
CA act std e [±]
(internal, control)		16 
CA activity [REA/g]
(internal, DIDS+AZ)		17 
CA act std e [±]
(internal, DIDS+AZ)		18 
pH
(total scale, Spectrophotometric)		19 
AT [µmol/kg]
(Potentiometric titration)		20 
DIC [µmol/kg]
(Coulometric titration)		21 
Temp [°C]		22 
Sal		23 
[HCO3]- [µmol/kg]
(Calculated using SWCO2 (Hunte...)		24 
CO2 [µmol/kg]
(Calculated using SWCO2 (Hunte...)		25 
[CO3]2- [µmol/kg]
(Calculated using SWCO2 (Hunte...)		26 
fCO2water_SST_wet [µatm]
(Calculated using SWCO2 (Hunte...)		27 
pCO2water_SST_wet [µatm]
(Calculated using SWCO2 (Hunte...)		28 
CSC flag
(Calculated using seacarb afte...)		29 
pH
(total scale, Calculated using...)		30 
CO2 [µmol/kg]
(Calculated using seacarb afte...)		31 
pCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)		32 
fCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)		33 
[HCO3]- [µmol/kg]
(Calculated using seacarb afte...)		34 
[CO3]2- [µmol/kg]
(Calculated using seacarb afte...)		35 
Omega Arg
(Calculated using seacarb afte...)		36 
Omega Cal
(Calculated using seacarb afte...)
Macrocystis pyrifera (macroalga)7.658.1066.324.088.052233.302011.8315.3035.001841.4013.63156.81342.14343.42158.0613.97377.78376.441840.32157.542.413.75
Macrocystis pyrifera (macroalga)9.008.1032.999.298.052233.302011.8315.3035.001841.4013.63156.81342.14343.42158.0613.97377.78376.441840.32157.542.413.75
Macrocystis pyrifera (macroalga)7.657.6580.4110.717.612203.002145.3916.7335.002040.0340.3565.011012.831016.60157.6141.581172.831168.742037.4866.331.021.58
Macrocystis pyrifera (macroalga)9.007.6546.812.887.612203.002145.3916.7335.002040.0340.3565.011012.831016.60157.6141.581172.831168.742037.4866.331.021.58
Macrocystis pyrifera (macroalga)7.659.0091.966.669.022609.151590.5615.9035.00876.790.68713.0917.0717.14159.020.7119.6019.53878.36711.4910.9116.96
Macrocystis pyrifera (macroalga)9.009.0058.7011.719.022609.151590.5615.9035.00876.790.68713.0917.0717.14159.020.7119.6019.53878.36711.4910.9116.96
Macrocystis pyrifera (macroalga)7.657.6555.862.3011.096.187.612203.002145.3916.7335.002040.0340.3565.011012.831016.60157.6141.581172.831168.742037.4866.331.021.58
Macrocystis pyrifera (macroalga)9.007.6565.152.1033.652.457.612203.002145.3916.7335.002040.0340.3565.011012.831016.60157.6141.581172.831168.742037.4866.331.021.58
Macrocystis pyrifera (macroalga)7.659.0055.080.4317.055.809.022609.151590.5615.9035.00876.790.68713.0917.0717.14159.020.7119.6019.53878.36711.4910.9116.96
Macrocystis pyrifera (macroalga)9.009.0054.369.9120.7510.149.022609.151590.5615.9035.00876.790.68713.0917.0717.14159.020.7119.6019.53878.36711.4910.9116.96
Macrocystis pyrifera (macroalga)8.103.840.328.420.998.052233.302011.8315.3035.001841.4013.63156.81342.14343.42158.0613.97377.78376.441840.32157.542.413.75
Macrocystis pyrifera (macroalga)7.651.730.467.671.627.612203.002145.3916.7335.002040.0340.3565.011012.831016.60157.6141.581172.831168.742037.4866.331.021.58
Macrocystis pyrifera (macroalga)9.002.010.458.791.809.022609.151590.5615.9035.00876.790.68713.0917.0717.14159.020.7119.6019.53878.36711.4910.9116.96
Macrocystis pyrifera (macroalga)7.657.671.6239261977.612203.002145.3916.7335.002040.0340.3565.011012.831016.60157.6141.581172.831168.742037.4866.331.021.58
Macrocystis pyrifera (macroalga)9.008.791.7977993579.022609.151590.5615.9035.00876.790.68713.0917.0717.14159.020.7119.6019.53878.36711.4910.9116.96
Macrocystis pyrifera (macroalga)7.657.652.880.7475975187.612203.002145.3916.7335.002040.0340.3565.011012.831016.60157.6141.581172.831168.742037.4866.331.021.58
Macrocystis pyrifera (macroalga)7.659.009.131.4908850149.022609.151590.5615.9035.00876.790.68713.0917.0717.14159.020.7119.6019.53878.36711.4910.9116.96
Macrocystis pyrifera (macroalga)9.007.653.270.6316936237.612203.002145.3916.7335.002040.0340.3565.011012.831016.60157.6141.581172.831168.742037.4866.331.021.58
Macrocystis pyrifera (macroalga)9.009.003.320.4348382099.022609.151590.5615.9035.00876.790.68713.0917.0717.14159.020.7119.6019.53878.36711.4910.9116.96