Citation:

ece3.709

Name: Elevated CO2 affects embryonic development and larval phototaxis in a temperate marine fish
Published: 2013
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Animalia; Behaviour; Chordata; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Development; Gobiusculus flavescens; Laboratory experiment; Nekton; North Atlantic; Pelagos; Reproduction; Single species; Temperate

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Abstract:

As an effect of anthropogenic CO2 emissions, the chemistry of the world's oceans is changing. Understanding how this will affect marine organisms and ecosystems are critical in predicting the impacts of this ongoing ocean acidification. Work on coral reef fishes has revealed dramatic effects of elevated oceanic CO2 on sensory responses and behavior. Such effects may be widespread but have almost exclusively been tested on tropical reef fishes. Here we test the effects elevated CO2 has on the reproduction and early life history stages of a temperate coastal goby with paternal care by allowing goby pairs to reproduce naturally in an aquarium with either elevated (ca 1400 µatm) CO2 or control seawater (ca 370 µatm CO2). Elevated CO2 did not affect the occurrence of spawning nor clutch size, but increased embryonic abnormalities and egg loss. Moreover, we found that elevated CO2 significantly affected the phototactic response of newly hatched larvae. Phototaxis is a vision-related fundamental behavior of many marine fishes, but has never before been tested in the context of ocean acidification. Our findings suggest that ocean acidification affects embryonic development and sensory responses in temperate fishes, with potentially important implications for fish recruitment.

Keyword(s):

Animalia; Behaviour; Chordata; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Development; Gobiusculus flavescens; Laboratory experiment; Nekton; North Atlantic; Pelagos; Reproduction; Single species; Temperate

Further details:

Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: 58.250000 * Longitude: 11.450000

Date/Time Start: 2010-07-20T00:00:00 * Date/Time End: 2010-08-24T00:00:00

Event(s):

Kristineberg * Latitude: 58.250000 * Longitude: 11.450000 * Date/Time Start: 2010-07-20T00:00:00 * Date/Time End: 2010-08-24T00:00:00 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-11-21.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Species	Species		Forsgren, Elisabet
2	Treatment	Treat		Forsgren, Elisabet
3	Identification	ID		Forsgren, Elisabet		aquarium
4	Incubation duration	Inc dur	days	Forsgren, Elisabet
5	Embryos	Embryos	#	Forsgren, Elisabet		total
6	Embryos	Embryos	%	Forsgren, Elisabet		normal
7	Embryos	Embryos	#	Forsgren, Elisabet		all abnormal and unfertilised
8	Embryos	Embryos	#	Forsgren, Elisabet		all abnormal incl upside down
9	Embryos	Embryos	%	Forsgren, Elisabet		all abnormal incl upside down
10	Embryos	Embryos	#	Forsgren, Elisabet		unfertilised
11	Embryos	Embryos	%	Forsgren, Elisabet		unfertilised
12	Embryos	Embryos	#	Forsgren, Elisabet		deformed w eyes, all
13	Embryos	Embryos	%	Forsgren, Elisabet		w eyes
14	Embryos	Embryos	#	Forsgren, Elisabet		developed eyes
15	Embryos	Embryos	%	Forsgren, Elisabet		large eyes
16	Embryos	Embryos	#	Forsgren, Elisabet		other (stopped early
17	Embryos	Embryos	%	Forsgren, Elisabet		stopped in between
18	Embryos	Embryos	#	Forsgren, Elisabet		upside down (not abnormal)
19	Embryos	Embryos	%	Forsgren, Elisabet		upside down
20	Duration, number of days	Duration	days	Forsgren, Elisabet		days past fertilisation, min
21	Duration, number of days	Duration	days	Forsgren, Elisabet		days past fertilisation, max
22	Abnormality, cumulative	Abn cum		Forsgren, Elisabet
23	Eggs	Eggs	#	Forsgren, Elisabet		day 1-2
24	Eggs	Eggs	#	Forsgren, Elisabet		day 9
25	Eggs	Eggs	#	Forsgren, Elisabet		lost
26	Percentage	Perc	%	Forsgren, Elisabet		lost
27	Eggs survived to hatching	Eggs survived hatch	%	Forsgren, Elisabet		remaining at d9
28	Phototactic response	Phototactic resp	%	Forsgren, Elisabet
29	Swimming duration	Swim dur	s	Forsgren, Elisabet		mean batch 1-3
30	Salinity	Sal		Forsgren, Elisabet
31	Temperature, water	Temp	°C	Forsgren, Elisabet
32	pH	pH		Forsgren, Elisabet	Potentiometric	total scale
33	pH, standard error	pH std e	±	Forsgren, Elisabet	Potentiometric	total scale
34	Alkalinity, total	AT	µmol/kg	Forsgren, Elisabet	Potentiometric titration
35	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Forsgren, Elisabet	Calculated using CO2SYS
36	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error	pCO2water_SST_wet std e	±	Forsgren, Elisabet	Calculated using CO2SYS
37	Bicarbonate ion	[HCO3]-	µmol/kg	Forsgren, Elisabet	Calculated using CO2SYS
38	Bicarbonate ion, standard error	[HCO3]- std e	±	Forsgren, Elisabet	Calculated using CO2SYS
39	Carbonate ion	[CO3]2-	µmol/kg	Forsgren, Elisabet	Calculated using CO2SYS
40	Carbonate ion, standard error	[CO3]2- std e	±	Forsgren, Elisabet	Calculated using CO2SYS
41	Calcite saturation state	Omega Cal		Forsgren, Elisabet	Calculated using CO2SYS
42	Calcite saturation state, standard error	Omega Cal std e	±	Forsgren, Elisabet	Calculated using CO2SYS
43	Aragonite saturation state	Omega Arg		Forsgren, Elisabet	Calculated using CO2SYS
44	Aragonite saturation state, standard error	Omega Arg std e	±	Forsgren, Elisabet	Calculated using CO2SYS
45	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
46	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
47	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
48	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
49	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
50	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
51	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
52	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
53	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

5589 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Species	2 Treat	3 ID (aquarium)	4 Inc dur [days]	5 Embryos [#] (total)	6 Embryos [%] (normal)	7 Embryos [#] (all abnormal and unfertilised)	8 Embryos [#] (all abnormal incl upside down)	9 Embryos [%] (all abnormal incl upside down)	10 Embryos [#] (unfertilised)	11 Embryos [%] (unfertilised)	12 Embryos [#] (deformed w eyes, all)	13 Embryos [%] (w eyes)	14 Embryos [#] (developed eyes)	15 Embryos [%] (large eyes)	16 Embryos [#] (other (stopped early)	17 Embryos [%] (stopped in between)	18 Embryos [#] (upside down (not abnormal))	19 Embryos [%] (upside down)	20 Duration [days] (days past fertilisation, min)	21 Duration [days] (days past fertilisation, max)	22 Abn cum	23 Eggs [#] (day 1-2)	24 Eggs [#] (day 9)	25 Eggs [#] (lost)	26 Perc [%] (lost)	27 Eggs survived hatch [%] (remaining at d9)	28 Phototactic resp [%]	29 Swim dur [s] (mean batch 1-3)	30 Sal	31 Temp [°C]	32 pH (total scale, Potentiometric)	33 pH std e [±] (total scale, Potentiometric)	34 AT [µmol/kg] (Potentiometric titration)	35 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	36 pCO2water_SST_wet std e [±] (Calculated using CO2SYS)	37 [HCO3]- [µmol/kg] (Calculated using CO2SYS)	38 [HCO3]- std e [±] (Calculated using CO2SYS)	39 [CO3]2- [µmol/kg] (Calculated using CO2SYS)	40 [CO3]2- std e [±] (Calculated using CO2SYS)	41 Omega Cal (Calculated using CO2SYS)	42 Omega Cal std e [±] (Calculated using CO2SYS)	43 Omega Arg (Calculated using CO2SYS)	44 Omega Arg std e [±] (Calculated using CO2SYS)	45 CSC flag (Calculated using seacarb afte...)	46 CO2 [µmol/kg] (Calculated using seacarb afte...)	47 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	48 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	49 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	50 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	51 DIC [µmol/kg] (Calculated using seacarb afte...)	52 Omega Arg (Calculated using seacarb afte...)	53 Omega Cal (Calculated using seacarb afte...)
Gobiusculus flavescens (fish)	Elevated CO2	1	9	2997	98.0	59	60	2.00	6	0.20	30	1.00	2	0.07	21	0.70	1	0.03											32	14.9	7.61	0.01	2300	1268	38	2152	4	60.3	1.5	1.47	0.04	0.94	0.02	8	47.35	1243.57	1239.13	2151.73	60.43	2259.51	0.94	1.47
Gobiusculus flavescens (fish)	Control	2	9	1570	97.0	46	47	2.99	5	0.32	13	0.83	0	0.00	28	1.78	1	0.06											32	14.9	8.10	0.01	2300	357	8	1895	6	165.2	2.5	4.01	0.06	2.56	0.04	8	13.50	354.67	353.41	1896.47	164.59	2074.56	2.55	4.00
Gobiusculus flavescens (fish)	Elevated CO2	3	9	1465	98.4	23	23	1.57	1	0.07	15	1.02	0	0.00	7	0.48	0	0.00											32	14.9	7.59	0.01	2300	1315	19	2157	2	58.1	0.7	1.41	0.02	0.90	0.01	8	49.72	1305.97	1301.31	2158.00	57.88	2265.59	0.90	1.41
Gobiusculus flavescens (fish)	Control	4	9	1403	97.9	29	30	2.14	2	0.14	11	0.78	0	0.00	16	1.14	1	0.07											32	14.9	8.09	0.01	2300	373	8	1907	6	160.2	2.4	3.89	0.06	2.49	0.04	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Elevated CO2	5	9	1617	89.4	170	172	10.64	24	1.48	69	4.27	3	0.19	77	4.76	2	0.12											32	14.9	7.58	0.01	2300	1365	26	2161	2	56.3	1.0	1.37	0.02	0.87	0.01	8	50.95	1338.27	1333.49	2161.03	56.64	2268.62	0.88	1.38
Gobiusculus flavescens (fish)	Control	6	9	1607	97.1	47	47	2.92	2	0.12	19	1.18	0	0.00	26	1.62	0	0.00											32	14.9	8.10	0.01	2300	363	7	1899	5	163.3	2.1	4.00	0.05	2.53	0.03	8	13.50	354.67	353.41	1896.47	164.59	2074.56	2.55	4.00
Gobiusculus flavescens (fish)	Elevated CO2	7	9	1291	89.9	50	131	10.15	11	0.85	17	1.32	0	0.00	22	1.70	81	6.27											32	14.9	7.42	0.02	2300	2012	109	2201	5	40.4	1.8	0.98	0.04	0.63	0.03	8	75.05	1971.10	1964.06	2202.04	39.93	2317.02	0.62	0.97
Gobiusculus flavescens (fish)	Control	8	9	1593	89.5	167	167	10.48	0	0.00	58	3.64	3	0.19	109	6.84	0	0.00											32	14.9	8.08	0.01	2300	376	4	1909	3	159.2	1.3	3.87	0.03	2.47	0.02	8	14.25	374.34	373.01	1911.55	158.43	2084.23	2.46	3.85
Gobiusculus flavescens (fish)	Control	9	9	2413	99.7	8	8	0.33	0	0.00	3	0.12	1	0.04	5	0.21	0	0.00											32	14.9	8.09	0.01	2300	371	7	1905	6	160.8	2.7	3.91	0.06	2.50	0.04	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Control	10	9	2839	97.7	56	64	2.25	2	0.07	24	0.85	3	0.11	30	1.06	8	0.28											32	14.9	8.10	0.01	2300	358	6	1896	5	164.8	2.0	4.01	0.05	2.56	0.03	8	13.50	354.67	353.41	1896.47	164.59	2074.56	2.55	4.00
Gobiusculus flavescens (fish)	Elevated CO2	11	9	631	99.0	6	6	0.95	0	0.00	3	0.48	1	0.16	3	0.48	0	0.00											32	14.9	7.59	0.01	2300	1324	46	2157	5	58.2	1.8	1.41	0.04	0.90	0.03	8	49.72	1305.97	1301.31	2158.00	57.88	2265.59	0.90	1.41
Gobiusculus flavescens (fish)	Elevated CO2	12	9	986	98.7	13	13	1.32	0	0.00	10	1.01	3	0.30	3	0.30	0	0.00											32	14.9	7.59	0.01	2300	1316	41	2157	4	58.4	1.6	1.42	0.04	0.91	0.02	8	49.72	1305.97	1301.31	2158.00	57.88	2265.59	0.90	1.41
Gobiusculus flavescens (fish)	Control	13	9	1415	99.1	12	13	0.92	1	0.07	6	0.42	3	0.21	5	0.35	1	0.07											32	14.9	8.09	0.01	2300	366	8	1902	6	162.4	2.5	3.95	0.06	2.52	0.04	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Control	14	9	1138	97.8	25	25	2.20	10	0.88	4	0.35	1	0.09	11	0.97	0	0.00											32	14.9	8.09	0.91	2300	373	7	1907	5	160.2	2.2	3.89	0.05	2.48	0.03	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Elevated CO2	15	9	1232	97.2	32	35	2.84	4	0.32	17	1.38	7	0.57	11	0.89	3	0.24											32	14.9	7.59	0.01	2300	1319	39	2157	4	58.2	1.5	1.42	0.04	0.90	0.02	8	49.72	1305.97	1301.31	2158.00	57.88	2265.59	0.90	1.41
Gobiusculus flavescens (fish)	Control	17	9	2350	99.1	15	20	0.85	0	0.00	5	0.21	1	0.04	10	0.43	5	0.21											32	14.9	8.09	0.01	2300	365	8	1901	6	162.7	2.5	3.95	0.06	2.52	0.04	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Elevated CO2	18	9	1146	97.8	24	25	2.18	6	0.52	4	0.35	1	0.09	14	1.22	1	0.09											32	14.9	7.60	0.01	2300	1294	38	2155	4	59.2	1.5	1.44	0.04	0.92	0.02	8	48.52	1274.41	1269.86	2154.90	59.14	2262.56	0.92	1.44
Gobiusculus flavescens (fish)	Control	19	9	1474	99.1	13	13	0.88	0	0.00	8	0.54	3	0.20	5	0.34	0	0.00											32	14.9	8.11	0.01	2300	354	7	1892	5	166.1	2.2	4.04	0.05	2.58	0.03	8	13.14	345.19	343.96	1888.76	167.74	2069.64	2.60	4.07
Gobiusculus flavescens (fish)	Control	21	9	1316	99.0	11	13	0.99	1	0.08	9	0.68	2	0.15	1	0.08	2	0.15											32	14.9	8.09	0.01	2300	372	7	1906	5	160.5	2.2	3.90	0.05	2.49	0.03	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Elevated CO2	22	9	1192	98.1	22	23	1.93	7	0.59	6	0.50	2	0.17	9	0.76	1	0.08											32	14.9	7.58	0.01	2300	1375	41	2162	4	56.1	1.5	1.36	0.04	0.87	0.02	8	50.95	1338.27	1333.49	2161.03	56.64	2268.62	0.88	1.38
Gobiusculus flavescens (fish)	Control	23	9	858	94.5	46	47	5.48	25	2.91	11	1.28	2	0.23	10	1.17	1	0.12											32	14.9	8.09	0.01	2300	369	7	1904	5	161.4	2.1	3.92	0.05	2.50	0.03	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Control	25	9	766	99.3	5	5	0.65	0	0.00	3	0.39	1	0.13	2	0.26	0	0.00											32	14.9	8.10	0.01	2300	360	8	1897	7	164.3	2.6	3.99	0.06	2.55	0.04	8	13.50	354.67	353.41	1896.47	164.59	2074.56	2.55	4.00
Gobiusculus flavescens (fish)	Elevated CO2	26	9	1492	88.0	172	179	12.00	64	4.29	5	0.34	4	0.27	103	6.90	7	0.47											32	14.9	7.42	0.03	2300	2063	161	2201	6	40.3	2.6	0.98	0.06	0.63	0.04	8	75.05	1971.10	1964.06	2202.04	39.93	2317.02	0.62	0.97
Gobiusculus flavescens (fish)	Control	27	9	1038	99.4	6	6	0.58	1	0.10	3	0.29	1	0.10	2	0.19	0	0.00											32	14.9	8.08	0.01	2300	378	7	1911	5	158.7	2.1	3.87	0.05	2.46	0.03	8	14.25	374.34	373.01	1911.55	158.43	2084.23	2.46	3.85
Gobiusculus flavescens (fish)	Elevated CO2	28	9	1464	78.1	320	320	21.86	4	0.27	5	0.34	1	0.07	311	21.24	0	0.00											32	14.9	7.58	0.01	2300	1350	56	2159	5	57.4	2.1	1.40	0.05	0.89	0.03	8	50.95	1338.27	1333.49	2161.03	56.64	2268.62	0.88	1.38
Gobiusculus flavescens (fish)	Elevated CO2	1																	0	1	0.20								32	14.9	7.61	0.01	2300	1268	38	2152	4	60.3	1.5	1.47	0.04	0.94	0.02	8	47.35	1243.57	1239.13	2151.73	60.43	2259.51	0.94	1.47
Gobiusculus flavescens (fish)	Control	2																	0	1	0.32								32	14.9	8.10	0.01	2300	357	8	1895	6	165.2	2.5	4.01	0.06	2.56	0.04	8	13.50	354.67	353.41	1896.47	164.59	2074.56	2.55	4.00
Gobiusculus flavescens (fish)	Elevated CO2	3																	0	1	0.07								32	14.9	7.59	0.01	2300	1315	19	2157	2	58.1	0.7	1.41	0.02	0.90	0.01	8	49.72	1305.97	1301.31	2158.00	57.88	2265.59	0.90	1.41
Gobiusculus flavescens (fish)	Control	4																	0	1	0.14								32	14.9	8.09	0.01	2300	373	8	1907	6	160.2	2.4	3.89	0.06	2.49	0.04	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Elevated CO2	5																	0	1	1.48								32	14.9	7.58	0.01	2300	1365	26	2161	2	56.3	1.0	1.37	0.02	0.87	0.01	8	50.95	1338.27	1333.49	2161.03	56.64	2268.62	0.88	1.38
Gobiusculus flavescens (fish)	Control	6																	0	1	0.12								32	14.9	8.10	0.01	2300	363	7	1899	5	163.3	2.1	4.00	0.05	2.53	0.03	8	13.50	354.67	353.41	1896.47	164.59	2074.56	2.55	4.00
Gobiusculus flavescens (fish)	Elevated CO2	7																	0	1	0.85								32	14.9	7.42	0.02	2300	2012	109	2201	5	40.4	1.8	0.98	0.04	0.63	0.03	8	75.05	1971.10	1964.06	2202.04	39.93	2317.02	0.62	0.97
Gobiusculus flavescens (fish)	Control	8																	0	1	0.00								32	14.9	8.08	0.01	2300	376	4	1909	3	159.2	1.3	3.87	0.03	2.47	0.02	8	14.25	374.34	373.01	1911.55	158.43	2084.23	2.46	3.85
Gobiusculus flavescens (fish)	Control	9																	0	1	0.00								32	14.9	8.09	0.01	2300	371	7	1905	6	160.8	2.7	3.91	0.06	2.50	0.04	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Control	10																	0	1	0.07								32	14.9	8.10	0.01	2300	358	6	1896	5	164.8	2.0	4.01	0.05	2.56	0.03	8	13.50	354.67	353.41	1896.47	164.59	2074.56	2.55	4.00
Gobiusculus flavescens (fish)	Elevated CO2	11																	0	1	0.00								32	14.9	7.59	0.01	2300	1324	46	2157	5	58.2	1.8	1.41	0.04	0.90	0.03	8	49.72	1305.97	1301.31	2158.00	57.88	2265.59	0.90	1.41
Gobiusculus flavescens (fish)	Elevated CO2	12																	0	1	0.00								32	14.9	7.59	0.01	2300	1316	41	2157	4	58.4	1.6	1.42	0.04	0.91	0.02	8	49.72	1305.97	1301.31	2158.00	57.88	2265.59	0.90	1.41
Gobiusculus flavescens (fish)	Control	13																	0	1	0.07								32	14.9	8.09	0.01	2300	366	8	1902	6	162.4	2.5	3.95	0.06	2.52	0.04	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Control	14																	0	1	0.88								32	14.9	8.09	0.91	2300	373	7	1907	5	160.2	2.2	3.89	0.05	2.48	0.03	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Elevated CO2	15																	0	1	0.32								32	14.9	7.59	0.01	2300	1319	39	2157	4	58.2	1.5	1.42	0.04	0.90	0.02	8	49.72	1305.97	1301.31	2158.00	57.88	2265.59	0.90	1.41
Gobiusculus flavescens (fish)	Control	17																	0	1	0.00								32	14.9	8.09	0.01	2300	365	8	1901	6	162.7	2.5	3.95	0.06	2.52	0.04	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Elevated CO2	18																	0	1	0.52								32	14.9	7.60	0.01	2300	1294	38	2155	4	59.2	1.5	1.44	0.04	0.92	0.02	8	48.52	1274.41	1269.86	2154.90	59.14	2262.56	0.92	1.44
Gobiusculus flavescens (fish)	Control	19																	0	1	0.00								32	14.9	8.11	0.01	2300	354	7	1892	5	166.1	2.2	4.04	0.05	2.58	0.03	8	13.14	345.19	343.96	1888.76	167.74	2069.64	2.60	4.07
Gobiusculus flavescens (fish)	Control	21																	0	1	0.08								32	14.9	8.09	0.01	2300	372	7	1906	5	160.5	2.2	3.90	0.05	2.49	0.03	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Elevated CO2	22																	0	1	0.59								32	14.9	7.58	0.01	2300	1375	41	2162	4	56.1	1.5	1.36	0.04	0.87	0.02	8	50.95	1338.27	1333.49	2161.03	56.64	2268.62	0.88	1.38
Gobiusculus flavescens (fish)	Control	23																	0	1	2.91								32	14.9	8.09	0.01	2300	369	7	1904	5	161.4	2.1	3.92	0.05	2.50	0.03	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Control	25																	0	1	0.00								32	14.9	8.10	0.01	2300	360	8	1897	7	164.3	2.6	3.99	0.06	2.55	0.04	8	13.50	354.67	353.41	1896.47	164.59	2074.56	2.55	4.00
Gobiusculus flavescens (fish)	Elevated CO2	26																	0	1	4.29								32	14.9	7.42	0.03	2300	2063	161	2201	6	40.3	2.6	0.98	0.06	0.63	0.04	8	75.05	1971.10	1964.06	2202.04	39.93	2317.02	0.62	0.97
Gobiusculus flavescens (fish)	Control	27																	0	1	0.10								32	14.9	8.08	0.01	2300	378	7	1911	5	158.7	2.1	3.87	0.05	2.46	0.03	8	14.25	374.34	373.01	1911.55	158.43	2084.23	2.46	3.85
Gobiusculus flavescens (fish)	Elevated CO2	28																	0	1	0.27								32	14.9	7.58	0.01	2300	1350	56	2159	5	57.4	2.1	1.40	0.05	0.89	0.03	8	50.95	1338.27	1333.49	2161.03	56.64	2268.62	0.88	1.38
Gobiusculus flavescens (fish)	Elevated CO2	1																	2	3	0.90								32	14.9	7.61	0.01	2300	1268	38	2152	4	60.3	1.5	1.47	0.04	0.94	0.02	8	47.35	1243.57	1239.13	2151.73	60.43	2259.51	0.94	1.47
Gobiusculus flavescens (fish)	Control	2																	2	3	2.10								32	14.9	8.10	0.01	2300	357	8	1895	6	165.2	2.5	4.01	0.06	2.56	0.04	8	13.50	354.67	353.41	1896.47	164.59	2074.56	2.55	4.00
Gobiusculus flavescens (fish)	Elevated CO2	3																	2	3	0.55								32	14.9	7.59	0.01	2300	1315	19	2157	2	58.1	0.7	1.41	0.02	0.90	0.01	8	49.72	1305.97	1301.31	2158.00	57.88	2265.59	0.90	1.41
Gobiusculus flavescens (fish)	Control	4																	2	3	1.28								32	14.9	8.09	0.01	2300	373	8	1907	6	160.2	2.4	3.89	0.06	2.49	0.04	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Elevated CO2	5																	2	3	6.25								32	14.9	7.58	0.01	2300	1365	26	2161	2	56.3	1.0	1.37	0.02	0.87	0.01	8	50.95	1338.27	1333.49	2161.03	56.64	2268.62	0.88	1.38
Gobiusculus flavescens (fish)	Control	6																	2	3	1.74								32	14.9	8.10	0.01	2300	363	7	1899	5	163.3	2.1	4.00	0.05	2.53	0.03	8	13.50	354.67	353.41	1896.47	164.59	2074.56	2.55	4.00
Gobiusculus flavescens (fish)	Elevated CO2	7																	2	3	2.56								32	14.9	7.42	0.02	2300	2012	109	2201	5	40.4	1.8	0.98	0.04	0.63	0.03	8	75.05	1971.10	1964.06	2202.04	39.93	2317.02	0.62	0.97
Gobiusculus flavescens (fish)	Control	8																	2	3	6.84								32	14.9	8.08	0.01	2300	376	4	1909	3	159.2	1.3	3.87	0.03	2.47	0.02	8	14.25	374.34	373.01	1911.55	158.43	2084.23	2.46	3.85
Gobiusculus flavescens (fish)	Control	9																	2	3	0.21								32	14.9	8.09	0.01	2300	371	7	1905	6	160.8	2.7	3.91	0.06	2.50	0.04	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Control	10																	2	3	1.13								32	14.9	8.10	0.01	2300	358	6	1896	5	164.8	2.0	4.01	0.05	2.56	0.03	8	13.50	354.67	353.41	1896.47	164.59	2074.56	2.55	4.00
Gobiusculus flavescens (fish)	Elevated CO2	11																	2	3	0.48								32	14.9	7.59	0.01	2300	1324	46	2157	5	58.2	1.8	1.41	0.04	0.90	0.03	8	49.72	1305.97	1301.31	2158.00	57.88	2265.59	0.90	1.41
Gobiusculus flavescens (fish)	Elevated CO2	12																	2	3	0.30								32	14.9	7.59	0.01	2300	1316	41	2157	4	58.4	1.6	1.42	0.04	0.91	0.02	8	49.72	1305.97	1301.31	2158.00	57.88	2265.59	0.90	1.41
Gobiusculus flavescens (fish)	Control	13																	2	3	0.42								32	14.9	8.09	0.01	2300	366	8	1902	6	162.4	2.5	3.95	0.06	2.52	0.04	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Control	14																	2	3	1.85								32	14.9	8.09	0.91	2300	373	7	1907	5	160.2	2.2	3.89	0.05	2.48	0.03	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Elevated CO2	15																	2	3	1.22								32	14.9	7.59	0.01	2300	1319	39	2157	4	58.2	1.5	1.42	0.04	0.90	0.02	8	49.72	1305.97	1301.31	2158.00	57.88	2265.59	0.90	1.41
Gobiusculus flavescens (fish)	Control	17																	2	3	0.43								32	14.9	8.09	0.01	2300	365	8	1901	6	162.7	2.5	3.95	0.06	2.52	0.04	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Elevated CO2	18																	2	3	1.75								32	14.9	7.60	0.01	2300	1294	38	2155	4	59.2	1.5	1.44	0.04	0.92	0.02	8	48.52	1274.41	1269.86	2154.90	59.14	2262.56	0.92	1.44
Gobiusculus flavescens (fish)	Control	19																	2	3	0.34								32	14.9	8.11	0.01	2300	354	7	1892	5	166.1	2.2	4.04	0.05	2.58	0.03	8	13.14	345.19	343.96	1888.76	167.74	2069.64	2.60	4.07
Gobiusculus flavescens (fish)	Control	21																	2	3	0.15								32	14.9	8.09	0.01	2300	372	7	1906	5	160.5	2.2	3.90	0.05	2.49	0.03	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Elevated CO2	22																	2	3	1.34								32	14.9	7.58	0.01	2300	1375	41	2162	4	56.1	1.5	1.36	0.04	0.87	0.02	8	50.95	1338.27	1333.49	2161.03	56.64	2268.62	0.88	1.38
Gobiusculus flavescens (fish)	Control	23																	2	3	4.08								32	14.9	8.09	0.01	2300	369	7	1904	5	161.4	2.1	3.92	0.05	2.50	0.03	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Control	25																	2	3	0.26								32	14.9	8.10	0.01	2300	360	8	1897	7	164.3	2.6	3.99	0.06	2.55	0.04	8	13.50	354.67	353.41	1896.47	164.59	2074.56	2.55	4.00
Gobiusculus flavescens (fish)	Elevated CO2	26																	2	3	11.19								32	14.9	7.42	0.03	2300	2063	161	2201	6	40.3	2.6	0.98	0.06	0.63	0.04	8	75.05	1971.10	1964.06	2202.04	39.93	2317.02	0.62	0.97
Gobiusculus flavescens (fish)	Control	27																	2	3	0.29								32	14.9	8.08	0.01	2300	378	7	1911	5	158.7	2.1	3.87	0.05	2.46	0.03	8	14.25	374.34	373.01	1911.55	158.43	2084.23	2.46	3.85
Gobiusculus flavescens (fish)	Elevated CO2	28																	2	3	21.52								32	14.9	7.58	0.01	2300	1350	56	2159	5	57.4	2.1	1.40	0.05	0.89	0.03	8	50.95	1338.27	1333.49	2161.03	56.64	2268.62	0.88	1.38
Gobiusculus flavescens (fish)	Elevated CO2	1																	4	5	1.84								32	14.9	7.61	0.01	2300	1268	38	2152	4	60.3	1.5	1.47	0.04	0.94	0.02	8	47.35	1243.57	1239.13	2151.73	60.43	2259.51	0.94	1.47
Gobiusculus flavescens (fish)	Control	2																	4	5	2.93								32	14.9	8.10	0.01	2300	357	8	1895	6	165.2	2.5	4.01	0.06	2.56	0.04	8	13.50	354.67	353.41	1896.47	164.59	2074.56	2.55	4.00
Gobiusculus flavescens (fish)	Elevated CO2	3																	4	5	1.57								32	14.9	7.59	0.01	2300	1315	19	2157	2	58.1	0.7	1.41	0.02	0.90	0.01	8	49.72	1305.97	1301.31	2158.00	57.88	2265.59	0.90	1.41
Gobiusculus flavescens (fish)	Control	4																	4	5	2.07								32	14.9	8.09	0.01	2300	373	8	1907	6	160.2	2.4	3.89	0.06	2.49	0.04	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Elevated CO2	5																	4	5	10.33								32	14.9	7.58	0.01	2300	1365	26	2161	2	56.3	1.0	1.37	0.02	0.87	0.01	8	50.95	1338.27	1333.49	2161.03	56.64	2268.62	0.88	1.38
Gobiusculus flavescens (fish)	Control	6																	4	5	2.92								32	14.9	8.10	0.01	2300	363	7	1899	5	163.3	2.1	4.00	0.05	2.53	0.03	8	13.50	354.67	353.41	1896.47	164.59	2074.56	2.55	4.00
Gobiusculus flavescens (fish)	Elevated CO2	7																	4	5	3.87								32	14.9	7.42	0.02	2300	2012	109	2201	5	40.4	1.8	0.98	0.04	0.63	0.03	8	75.05	1971.10	1964.06	2202.04	39.93	2317.02	0.62	0.97
Gobiusculus flavescens (fish)	Control	8																	4	5	10.30								32	14.9	8.08	0.01	2300	376	4	1909	3	159.2	1.3	3.87	0.03	2.47	0.02	8	14.25	374.34	373.01	1911.55	158.43	2084.23	2.46	3.85
Gobiusculus flavescens (fish)	Control	9																	4	5	0.29								32	14.9	8.09	0.01	2300	371	7	1905	6	160.8	2.7	3.91	0.06	2.50	0.04	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Control	10																	4	5	1.87								32	14.9	8.10	0.01	2300	358	6	1896	5	164.8	2.0	4.01	0.05	2.56	0.03	8	13.50	354.67	353.41	1896.47	164.59	2074.56	2.55	4.00
Gobiusculus flavescens (fish)	Elevated CO2	11																	4	5	0.79								32	14.9	7.59	0.01	2300	1324	46	2157	5	58.2	1.8	1.41	0.04	0.90	0.03	8	49.72	1305.97	1301.31	2158.00	57.88	2265.59	0.90	1.41
Gobiusculus flavescens (fish)	Elevated CO2	12																	4	5	1.01								32	14.9	7.59	0.01	2300	1316	41	2157	4	58.4	1.6	1.42	0.04	0.91	0.02	8	49.72	1305.97	1301.31	2158.00	57.88	2265.59	0.90	1.41
Gobiusculus flavescens (fish)	Control	13																	4	5	0.64								32	14.9	8.09	0.01	2300	366	8	1902	6	162.4	2.5	3.95	0.06	2.52	0.04	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Control	14																	4	5	2.11								32	14.9	8.09	0.91	2300	373	7	1907	5	160.2	2.2	3.89	0.05	2.48	0.03	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Elevated CO2	15																	4	5	2.03								32	14.9	7.59	0.01	2300	1319	39	2157	4	58.2	1.5	1.42	0.04	0.90	0.02	8	49.72	1305.97	1301.31	2158.00	57.88	2265.59	0.90	1.41
Gobiusculus flavescens (fish)	Control	17																	4	5	0.60								32	14.9	8.09	0.01	2300	365	8	1901	6	162.7	2.5	3.95	0.06	2.52	0.04	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Elevated CO2	18																	4	5	2.01								32	14.9	7.60	0.01	2300	1294	38	2155	4	59.2	1.5	1.44	0.04	0.92	0.02	8	48.52	1274.41	1269.86	2154.90	59.14	2262.56	0.92	1.44
Gobiusculus flavescens (fish)	Control	19																	4	5	0.68								32	14.9	8.11	0.01	2300	354	7	1892	5	166.1	2.2	4.04	0.05	2.58	0.03	8	13.14	345.19	343.96	1888.76	167.74	2069.64	2.60	4.07
Gobiusculus flavescens (fish)	Control	21																	4	5	0.68								32	14.9	8.09	0.01	2300	372	7	1906	5	160.5	2.2	3.90	0.05	2.49	0.03	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Elevated CO2	22																	4	5	1.68								32	14.9	7.58	0.01	2300	1375	41	2162	4	56.1	1.5	1.36	0.04	0.87	0.02	8	50.95	1338.27	1333.49	2161.03	56.64	2268.62	0.88	1.38
Gobiusculus flavescens (fish)	Control	23																	4	5	5.13								32	14.9	8.09	0.01	2300	369	7	1904	5	161.4	2.1	3.92	0.05	2.50	0.03	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Control	25																	4	5	0.52								32	14.9	8.10	0.01	2300	360	8	1897	7	164.3	2.6	3.99	0.06	2.55	0.04	8	13.50	354.67	353.41	1896.47	164.59	2074.56	2.55	4.00
Gobiusculus flavescens (fish)	Elevated CO2	26																	4	5	11.26								32	14.9	7.42	0.03	2300	2063	161	2201	6	40.3	2.6	0.98	0.06	0.63	0.04	8	75.05	1971.10	1964.06	2202.04	39.93	2317.02	0.62	0.97
Gobiusculus flavescens (fish)	Control	27																	4	5	0.48								32	14.9	8.08	0.01	2300	378	7	1911	5	158.7	2.1	3.87	0.05	2.46	0.03	8	14.25	374.34	373.01	1911.55	158.43	2084.23	2.46	3.85
Gobiusculus flavescens (fish)	Elevated CO2	28																	4	5	21.79								32	14.9	7.58	0.01	2300	1350	56	2159	5	57.4	2.1	1.40	0.05	0.89	0.03	8	50.95	1338.27	1333.49	2161.03	56.64	2268.62	0.88	1.38
Gobiusculus flavescens (fish)	Elevated CO2	1																	6	8	1.90								32	14.9	7.61	0.01	2300	1268	38	2152	4	60.3	1.5	1.47	0.04	0.94	0.02	8	47.35	1243.57	1239.13	2151.73	60.43	2259.51	0.94	1.47
Gobiusculus flavescens (fish)	Control	2																	6	8	2.93								32	14.9	8.10	0.01	2300	357	8	1895	6	165.2	2.5	4.01	0.06	2.56	0.04	8	13.50	354.67	353.41	1896.47	164.59	2074.56	2.55	4.00
Gobiusculus flavescens (fish)	Elevated CO2	3																	6	8	1.57								32	14.9	7.59	0.01	2300	1315	19	2157	2	58.1	0.7	1.41	0.02	0.90	0.01	8	49.72	1305.97	1301.31	2158.00	57.88	2265.59	0.90	1.41
Gobiusculus flavescens (fish)	Control	4																	6	8	2.07								32	14.9	8.09	0.01	2300	373	8	1907	6	160.2	2.4	3.89	0.06	2.49	0.04	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Elevated CO2	5																	6	8	10.51								32	14.9	7.58	0.01	2300	1365	26	2161	2	56.3	1.0	1.37	0.02	0.87	0.01	8	50.95	1338.27	1333.49	2161.03	56.64	2268.62	0.88	1.38
Gobiusculus flavescens (fish)	Control	6																	6	8	2.92								32	14.9	8.10	0.01	2300	363	7	1899	5	163.3	2.1	4.00	0.05	2.53	0.03	8	13.50	354.67	353.41	1896.47	164.59	2074.56	2.55	4.00
Gobiusculus flavescens (fish)	Elevated CO2	7																	6	8	3.87								32	14.9	7.42	0.02	2300	2012	109	2201	5	40.4	1.8	0.98	0.04	0.63	0.03	8	75.05	1971.10	1964.06	2202.04	39.93	2317.02	0.62	0.97
Gobiusculus flavescens (fish)	Control	8																	6	8	10.48								32	14.9	8.08	0.01	2300	376	4	1909	3	159.2	1.3	3.87	0.03	2.47	0.02	8	14.25	374.34	373.01	1911.55	158.43	2084.23	2.46	3.85
Gobiusculus flavescens (fish)	Control	9																	6	8	0.33								32	14.9	8.09	0.01	2300	371	7	1905	6	160.8	2.7	3.91	0.06	2.50	0.04	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Control	10																	6	8	1.97								32	14.9	8.10	0.01	2300	358	6	1896	5	164.8	2.0	4.01	0.05	2.56	0.03	8	13.50	354.67	353.41	1896.47	164.59	2074.56	2.55	4.00
Gobiusculus flavescens (fish)	Elevated CO2	11																	6	8	0.95								32	14.9	7.59	0.01	2300	1324	46	2157	5	58.2	1.8	1.41	0.04	0.90	0.03	8	49.72	1305.97	1301.31	2158.00	57.88	2265.59	0.90	1.41
Gobiusculus flavescens (fish)	Elevated CO2	12																	6	8	1.32								32	14.9	7.59	0.01	2300	1316	41	2157	4	58.4	1.6	1.42	0.04	0.91	0.02	8	49.72	1305.97	1301.31	2158.00	57.88	2265.59	0.90	1.41
Gobiusculus flavescens (fish)	Control	13																	6	8	0.85								32	14.9	8.09	0.01	2300	366	8	1902	6	162.4	2.5	3.95	0.06	2.52	0.04	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Control	14																	6	8	2.20								32	14.9	8.09	0.91	2300	373	7	1907	5	160.2	2.2	3.89	0.05	2.48	0.03	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Elevated CO2	15																	6	8	2.60								32	14.9	7.59	0.01	2300	1319	39	2157	4	58.2	1.5	1.42	0.04	0.90	0.02	8	49.72	1305.97	1301.31	2158.00	57.88	2265.59	0.90	1.41
Gobiusculus flavescens (fish)	Control	17																	6	8	0.64								32	14.9	8.09	0.01	2300	365	8	1901	6	162.7	2.5	3.95	0.06	2.52	0.04	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Elevated CO2	18																	6	8	2.09								32	14.9	7.60	0.01	2300	1294	38	2155	4	59.2	1.5	1.44	0.04	0.92	0.02	8	48.52	1274.41	1269.86	2154.90	59.14	2262.56	0.92	1.44
Gobiusculus flavescens (fish)	Control	19																	6	8	0.88								32	14.9	8.11	0.01	2300	354	7	1892	5	166.1	2.2	4.04	0.05	2.58	0.03	8	13.14	345.19	343.96	1888.76	167.74	2069.64	2.60	4.07
Gobiusculus flavescens (fish)	Control	21																	6	8	0.84								32	14.9	8.09	0.01	2300	372	7	1906	5	160.5	2.2	3.90	0.05	2.49	0.03	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Elevated CO2	22																	6	8	1.85								32	14.9	7.58	0.01	2300	1375	41	2162	4	56.1	1.5	1.36	0.04	0.87	0.02	8	50.95	1338.27	1333.49	2161.03	56.64	2268.62	0.88	1.38
Gobiusculus flavescens (fish)	Control	23																	6	8	5.36								32	14.9	8.09	0.01	2300	369	7	1904	5	161.4	2.1	3.92	0.05	2.50	0.03	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Control	25																	6	8	0.65								32	14.9	8.10	0.01	2300	360	8	1897	7	164.3	2.6	3.99	0.06	2.55	0.04	8	13.50	354.67	353.41	1896.47	164.59	2074.56	2.55	4.00
Gobiusculus flavescens (fish)	Elevated CO2	26																	6	8	11.53								32	14.9	7.42	0.03	2300	2063	161	2201	6	40.3	2.6	0.98	0.06	0.63	0.04	8	75.05	1971.10	1964.06	2202.04	39.93	2317.02	0.62	0.97
Gobiusculus flavescens (fish)	Control	27																	6	8	0.58								32	14.9	8.08	0.01	2300	378	7	1911	5	158.7	2.1	3.87	0.05	2.46	0.03	8	14.25	374.34	373.01	1911.55	158.43	2084.23	2.46	3.85
Gobiusculus flavescens (fish)	Elevated CO2	28																	6	8	21.86								32	14.9	7.58	0.01	2300	1350	56	2159	5	57.4	2.1	1.40	0.05	0.89	0.03	8	50.95	1338.27	1333.49	2161.03	56.64	2268.62	0.88	1.38
Gobiusculus flavescens (fish)	Elevated CO2	1																				3048	2997	51	1.67	98.33			32	14.9	7.61	0.01	2300	1268	38	2152	4	60.3	1.5	1.47	0.04	0.94	0.02	8	47.35	1243.57	1239.13	2151.73	60.43	2259.51	0.94	1.47
Gobiusculus flavescens (fish)	Control	2																				1719	1570	149	8.67	91.33			32	14.9	8.10	0.01	2300	357	8	1895	6	165.2	2.5	4.01	0.06	2.56	0.04	8	13.50	354.67	353.41	1896.47	164.59	2074.56	2.55	4.00
Gobiusculus flavescens (fish)	Elevated CO2	3																				1545	1465	80	5.18	94.82			32	14.9	7.59	0.01	2300	1315	19	2157	2	58.1	0.7	1.41	0.02	0.90	0.01	8	49.72	1305.97	1301.31	2158.00	57.88	2265.59	0.90	1.41
Gobiusculus flavescens (fish)	Control	4																				1569	1403	166	10.58	89.42			32	14.9	8.09	0.01	2300	373	8	1907	6	160.2	2.4	3.89	0.06	2.49	0.04	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Elevated CO2	5																				1877	1617	260	13.85	86.15			32	14.9	7.58	0.01	2300	1365	26	2161	2	56.3	1.0	1.37	0.02	0.87	0.01	8	50.95	1338.27	1333.49	2161.03	56.64	2268.62	0.88	1.38
Gobiusculus flavescens (fish)	Control	6																				1758	1607	151	8.59	91.41			32	14.9	8.10	0.01	2300	363	7	1899	5	163.3	2.1	4.00	0.05	2.53	0.03	8	13.50	354.67	353.41	1896.47	164.59	2074.56	2.55	4.00
Gobiusculus flavescens (fish)	Elevated CO2	7																				1407	1291	116	8.24	91.76			32	14.9	7.42	0.02	2300	2012	109	2201	5	40.4	1.8	0.98	0.04	0.63	0.03	8	75.05	1971.10	1964.06	2202.04	39.93	2317.02	0.62	0.97
Gobiusculus flavescens (fish)	Control	8																				1684	1593	91	5.40	94.60			32	14.9	8.08	0.01	2300	376	4	1909	3	159.2	1.3	3.87	0.03	2.47	0.02	8	14.25	374.34	373.01	1911.55	158.43	2084.23	2.46	3.85
Gobiusculus flavescens (fish)	Control	9																				2504	2413	91	3.63	96.37			32	14.9	8.09	0.01	2300	371	7	1905	6	160.8	2.7	3.91	0.06	2.50	0.04	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Control	10																				2861	2839	22	0.77	99.23			32	14.9	8.10	0.01	2300	358	6	1896	5	164.8	2.0	4.01	0.05	2.56	0.03	8	13.50	354.67	353.41	1896.47	164.59	2074.56	2.55	4.00
Gobiusculus flavescens (fish)	Elevated CO2	11																				1266	631	635	50.16	49.84			32	14.9	7.59	0.01	2300	1324	46	2157	5	58.2	1.8	1.41	0.04	0.90	0.03	8	49.72	1305.97	1301.31	2158.00	57.88	2265.59	0.90	1.41
Gobiusculus flavescens (fish)	Elevated CO2	12																				1060	986	74	6.98	93.02			32	14.9	7.59	0.01	2300	1316	41	2157	4	58.4	1.6	1.42	0.04	0.91	0.02	8	49.72	1305.97	1301.31	2158.00	57.88	2265.59	0.90	1.41
Gobiusculus flavescens (fish)	Control	13																				1484	1415	69	4.65	95.35			32	14.9	8.09	0.01	2300	366	8	1902	6	162.4	2.5	3.95	0.06	2.52	0.04	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Control	14																				1203	1138	65	5.40	94.60			32	14.9	8.09	0.91	2300	373	7	1907	5	160.2	2.2	3.89	0.05	2.48	0.03	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Elevated CO2	15																				1397	1232	165	11.81	88.19			32	14.9	7.59	0.01	2300	1319	39	2157	4	58.2	1.5	1.42	0.04	0.90	0.02	8	49.72	1305.97	1301.31	2158.00	57.88	2265.59	0.90	1.41
Gobiusculus flavescens (fish)	Control	17																				2385	2350	35	1.47	98.53			32	14.9	8.09	0.01	2300	365	8	1901	6	162.7	2.5	3.95	0.06	2.52	0.04	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Elevated CO2	18																				1259	1146	113	8.98	91.02			32	14.9	7.60	0.01	2300	1294	38	2155	4	59.2	1.5	1.44	0.04	0.92	0.02	8	48.52	1274.41	1269.86	2154.90	59.14	2262.56	0.92	1.44
Gobiusculus flavescens (fish)	Control	19																				1484	1474	10	0.67	99.33			32	14.9	8.11	0.01	2300	354	7	1892	5	166.1	2.2	4.04	0.05	2.58	0.03	8	13.14	345.19	343.96	1888.76	167.74	2069.64	2.60	4.07
Gobiusculus flavescens (fish)	Control	21																				1418	1316	102	7.19	92.81			32	14.9	8.09	0.01	2300	372	7	1906	5	160.5	2.2	3.90	0.05	2.49	0.03	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Elevated CO2	22																				1341	1192	149	11.11	88.89			32	14.9	7.58	0.01	2300	1375	41	2162	4	56.1	1.5	1.36	0.04	0.87	0.02	8	50.95	1338.27	1333.49	2161.03	56.64	2268.62	0.88	1.38
Gobiusculus flavescens (fish)	Control	23																				1091	858	233	21.36	78.64			32	14.9	8.09	0.01	2300	369	7	1904	5	161.4	2.1	3.92	0.05	2.50	0.03	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Control	25																				845	766	79	9.35	90.65			32	14.9	8.10	0.01	2300	360	8	1897	7	164.3	2.6	3.99	0.06	2.55	0.04	8	13.50	354.67	353.41	1896.47	164.59	2074.56	2.55	4.00
Gobiusculus flavescens (fish)	Elevated CO2	26																				1988	1492	496	24.95	75.05			32	14.9	7.42	0.03	2300	2063	161	2201	6	40.3	2.6	0.98	0.06	0.63	0.04	8	75.05	1971.10	1964.06	2202.04	39.93	2317.02	0.62	0.97
Gobiusculus flavescens (fish)	Control	27																				1055	1038	17	1.61	98.39			32	14.9	8.08	0.01	2300	378	7	1911	5	158.7	2.1	3.87	0.05	2.46	0.03	8	14.25	374.34	373.01	1911.55	158.43	2084.23	2.46	3.85
Gobiusculus flavescens (fish)	Elevated CO2	28																				1755	1464	291	16.58	83.42			32	14.9	7.58	0.01	2300	1350	56	2159	5	57.4	2.1	1.40	0.05	0.89	0.03	8	50.95	1338.27	1333.49	2161.03	56.64	2268.62	0.88	1.38
Gobiusculus flavescens (fish)	Elevated CO2	1																									86.67	28	32	14.9	7.61	0.01	2300	1268	38	2152	4	60.3	1.5	1.47	0.04	0.94	0.02	8	47.35	1243.57	1239.13	2151.73	60.43	2259.51	0.94	1.47
Gobiusculus flavescens (fish)	Control	2																									10.00	82	32	14.9	8.10	0.01	2300	357	8	1895	6	165.2	2.5	4.01	0.06	2.56	0.04	8	13.50	354.67	353.41	1896.47	164.59	2074.56	2.55	4.00
Gobiusculus flavescens (fish)	Elevated CO2	3																									80.00	27	32	14.9	7.59	0.01	2300	1315	19	2157	2	58.1	0.7	1.41	0.02	0.90	0.01	8	49.72	1305.97	1301.31	2158.00	57.88	2265.59	0.90	1.41
Gobiusculus flavescens (fish)	Control	4																									50.00		32	14.9	8.09	0.01	2300	373	8	1907	6	160.2	2.4	3.89	0.06	2.49	0.04	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Elevated CO2	5																									80.00	24	32	14.9	7.58	0.01	2300	1365	26	2161	2	56.3	1.0	1.37	0.02	0.87	0.01	8	50.95	1338.27	1333.49	2161.03	56.64	2268.62	0.88	1.38
Gobiusculus flavescens (fish)	Control	6																									26.67	54	32	14.9	8.10	0.01	2300	363	7	1899	5	163.3	2.1	4.00	0.05	2.53	0.03	8	13.50	354.67	353.41	1896.47	164.59	2074.56	2.55	4.00
Gobiusculus flavescens (fish)	Elevated CO2	7																									13.33		32	14.9	7.42	0.02	2300	2012	109	2201	5	40.4	1.8	0.98	0.04	0.63	0.03	8	75.05	1971.10	1964.06	2202.04	39.93	2317.02	0.62	0.97
Gobiusculus flavescens (fish)	Control	8																									40.00	44	32	14.9	8.08	0.01	2300	376	4	1909	3	159.2	1.3	3.87	0.03	2.47	0.02	8	14.25	374.34	373.01	1911.55	158.43	2084.23	2.46	3.85
Gobiusculus flavescens (fish)	Control	9																									56.67	70	32	14.9	8.09	0.01	2300	371	7	1905	6	160.8	2.7	3.91	0.06	2.50	0.04	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Control	10																									60.00	39	32	14.9	8.10	0.01	2300	358	6	1896	5	164.8	2.0	4.01	0.05	2.56	0.03	8	13.50	354.67	353.41	1896.47	164.59	2074.56	2.55	4.00
Gobiusculus flavescens (fish)	Elevated CO2	11																									73.33	43	32	14.9	7.59	0.01	2300	1324	46	2157	5	58.2	1.8	1.41	0.04	0.90	0.03	8	49.72	1305.97	1301.31	2158.00	57.88	2265.59	0.90	1.41
Gobiusculus flavescens (fish)	Elevated CO2	12																									53.33		32	14.9	7.59	0.01	2300	1316	41	2157	4	58.4	1.6	1.42	0.04	0.91	0.02	8	49.72	1305.97	1301.31	2158.00	57.88	2265.59	0.90	1.41
Gobiusculus flavescens (fish)	Control	13																									36.67		32	14.9	8.09	0.01	2300	366	8	1902	6	162.4	2.5	3.95	0.06	2.52	0.04	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Control	14																									36.67	102	32	14.9	8.09	0.91	2300	373	7	1907	5	160.2	2.2	3.89	0.05	2.48	0.03	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Elevated CO2	15																									50.00		32	14.9	7.59	0.01	2300	1319	39	2157	4	58.2	1.5	1.42	0.04	0.90	0.02	8	49.72	1305.97	1301.31	2158.00	57.88	2265.59	0.90	1.41
Gobiusculus flavescens (fish)	Control	17																									23.33		32	14.9	8.09	0.01	2300	365	8	1901	6	162.7	2.5	3.95	0.06	2.52	0.04	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Elevated CO2	18																									56.67	54	32	14.9	7.60	0.01	2300	1294	38	2155	4	59.2	1.5	1.44	0.04	0.92	0.02	8	48.52	1274.41	1269.86	2154.90	59.14	2262.56	0.92	1.44
Gobiusculus flavescens (fish)	Control	19																									16.67		32	14.9	8.11	0.01	2300	354	7	1892	5	166.1	2.2	4.04	0.05	2.58	0.03	8	13.14	345.19	343.96	1888.76	167.74	2069.64	2.60	4.07
Gobiusculus flavescens (fish)	Control	21																									86.67		32	14.9	8.09	0.01	2300	372	7	1906	5	160.5	2.2	3.90	0.05	2.49	0.03	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Elevated CO2	22																									90.00		32	14.9	7.58	0.01	2300	1375	41	2162	4	56.1	1.5	1.36	0.04	0.87	0.02	8	50.95	1338.27	1333.49	2161.03	56.64	2268.62	0.88	1.38
Gobiusculus flavescens (fish)	Control	23																									23.33		32	14.9	8.09	0.01	2300	369	7	1904	5	161.4	2.1	3.92	0.05	2.50	0.03	8	13.87	364.39	363.09	1904.07	161.49	2079.43	2.50	3.92
Gobiusculus flavescens (fish)	Control	25																									30.00	57	32	14.9	8.10	0.01	2300	360	8	1897	7	164.3	2.6	3.99	0.06	2.55	0.04	8	13.50	354.67	353.41	1896.47	164.59	2074.56	2.55	4.00
Gobiusculus flavescens (fish)	Elevated CO2	26																									53.33	57	32	14.9	7.42	0.03	2300	2063	161	2201	6	40.3	2.6	0.98	0.06	0.63	0.04	8	75.05	1971.10	1964.06	2202.04	39.93	2317.02	0.62	0.97
Gobiusculus flavescens (fish)	Control	27																									6.67		32	14.9	8.08	0.01	2300	378	7	1911	5	158.7	2.1	3.87	0.05	2.46	0.03	8	14.25	374.34	373.01	1911.55	158.43	2084.23	2.46	3.85
Gobiusculus flavescens (fish)	Elevated CO2	28																									50.00	45	32	14.9	7.58	0.01	2300	1350	56	2159	5	57.4	2.1	1.40	0.05	0.89	0.03	8	50.95	1338.27	1333.49	2161.03	56.64	2268.62	0.88	1.38