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Ellis, Robert P; Spicer, John I; Byrne, Jonathan J; Sommer, Ulf; Viant, Mark R; White, Daniel; Widdicombe, Steve (2014): 1H NMR Metabolomics Reveals Contrasting Response by Male and Female Mussels Exposed to Reduced Seawater pH, Increased Temperature, and a Pathogen [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.838938, Supplement to: Ellis, RP et al. (2014): 1H NMR Metabolomics Reveals Contrasting Response by Male and Female Mussels Exposed to Reduced Seawater pH, Increased Temperature, and a Pathogen. Environmental Science & Technology, 48(12), 7044-7052, https://doi.org/10.1021/es501601w

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Abstract:
Human activities are fundamentally altering the chemistry of the world's oceans. Ocean acidification (OA) is occurring against a background of warming and an increasing occurrence of disease outbreaks, posing a significant threat to marine organisms, communities, and ecosystems. In the current study, 1H NMR spectroscopy was used to investigate the response of the blue mussel, Mytilus edulis, to a 90-day exposure to reduced seawater pH and increased temperature, followed by a subsequent pathogenic challenge. Analysis of the metabolome revealed significant differences between male and female organisms. Furthermore, males and females are shown to respond differently to environmental stress. While males were significantly affected by reduced seawater pH, increased temperature, and a bacterial challenge, it was only a reduction in seawater pH that impacted females. Despite impacting males and females differently, stressors seem to act via a generalized stress response impacting both energy metabolism and osmotic balance in both sexes. This study therefore has important implications for the interpretation of metabolomic data in mussels, as well as the impact of environmental stress in marine invertebrates in general.
Keyword(s):
Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Laboratory experiment; Mollusca; Mortality/Survival; Mytilus edulis; North Atlantic; Single species; Temperate; Temperature
Further details:
Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0 [webpage]. https://cran.r-project.org/package=seacarb
Project(s):
Ocean Acidification International Coordination Centre (OA-ICC)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-11-17.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1SpeciesSpeciesEllis, Robert P
2pHpHEllis, Robert P
3Temperature, waterTemp°CEllis, Robert P
4IndividualsInd#Ellis, Robert Pdead
5MortalityMortality%Ellis, Robert P
6pHpHEllis, Robert PPotentiometricNBS scale
7pH, standard errorpH std e±Ellis, Robert PPotentiometricNBS scale
8Temperature, waterTemp°CEllis, Robert P
9Temperature, water, standard errorT std e±Ellis, Robert P
10SalinitySalEllis, Robert P
11Salinity, standard errorSal std e±Ellis, Robert P
12Alkalinity, totalATµmol/kgEllis, Robert PPotentiometric titration
13Alkalinity, total, standard errorAT std e±Ellis, Robert PPotentiometric titration
14Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmEllis, Robert PCalculated using CO2SYS
15Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard errorpCO2water_SST_wet std e±Ellis, Robert PCalculated using CO2SYS
16Calcite saturation stateOmega CalEllis, Robert PCalculated using CO2SYS
17Calcite saturation state, standard errorOmega Cal std e±Ellis, Robert PCalculated using CO2SYS
18Aragonite saturation stateOmega ArgEllis, Robert PCalculated using CO2SYS
19Aragonite saturation state, standard errorOmega Arg std e±Ellis, Robert PCalculated using CO2SYS
20Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
21pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
22Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
23Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
24Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
25Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
26Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
27Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
28Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
29Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
290 data points

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