Citation:

srep06218

Name: Ocean acidification impacts mussel control on biomineralisation
Published: 2014-10-31
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Growth/Morphology; Laboratory experiment; Mollusca; Mytilus edulis; North Atlantic; Other metabolic rates; Single species; Temperate

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Abstract:

Ocean acidification is altering the oceanic carbonate saturation state and threatening the survival of marine calcifying organisms. Production of their calcium carbonate exoskeletons is dependent not only on the environmental seawater carbonate chemistry but also the ability to produce biominerals through proteins. We present shell growth and structural responses by the economically important marine calcifier Mytilus edulis to ocean acidification scenarios (380, 550, 750, 1000 µatm pCO2). After six months of incubation at 750 µatm pCO2, reduced carbonic anhydrase protein activity and shell growth occurs in M. edulis. Beyond that, at 1000 µatm pCO2, biomineralisation continued but with compensated metabolism of proteins and increased calcite growth. Mussel growth occurs at a cost to the structural integrity of the shell due to structural disorientation of calcite crystals. This loss of structural integrity could impact mussel shell strength and reduce protection from predators and changing environments.

Keyword(s):

Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Growth/Morphology; Laboratory experiment; Mollusca; Mytilus edulis; North Atlantic; Other metabolic rates; Single species; Temperate

Further details:

Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-10-30.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Species	Species		Fitzer, Susan C
2	Image number/name	Image no/name		Fitzer, Susan C		shell
3	Sample ID	Sample ID		Fitzer, Susan C		shell
4	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Fitzer, Susan C		treatment
5	Temperature, water	Temp	°C	Fitzer, Susan C		treatment
6	Growth rate	µ	mm/month	Fitzer, Susan C		shell
7	Growth rate	µ	mm/month	Fitzer, Susan C		calcite
8	Growth rate	µ	mm/month	Fitzer, Susan C		aragonite
9	Date	Date		Fitzer, Susan C		analysed
10	Sample ID	Sample ID		Fitzer, Susan C		test
11	Carbonic anhydrase activity	CA activity	EU/ml	Fitzer, Susan C		eExtrapallial fluid
12	Wet mass	Wet m	g	Fitzer, Susan C		mantle tissue
13	Carbonic anhydrase activity, per tissue weight	CA act	EU/mg	Fitzer, Susan C		mantle tissue
14	Salinity	Sal		Fitzer, Susan C
15	Salinity, standard deviation	Sal std dev	±	Fitzer, Susan C
16	Oxygen saturation	O2 sat	%	Fitzer, Susan C
17	Oxygen, standard deviation	O2 std dev	±	Fitzer, Susan C
18	Temperature, water	Temp	°C	Fitzer, Susan C
19	Temperature, water, standard deviation	Temp std dev	±	Fitzer, Susan C
20	pH	pH		Fitzer, Susan C	Calculated using CO2SYS	total scale
21	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Fitzer, Susan C
22	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Fitzer, Susan C
23	Alkalinity, total	AT	µmol/kg	Fitzer, Susan C	Potentiometric titration
24	Alkalinity, total, standard deviation	AT std dev	±	Fitzer, Susan C	Potentiometric titration
25	Bicarbonate ion	[HCO3]-	µmol/kg	Fitzer, Susan C	Calculated using CO2SYS
26	Carbonate ion	[CO3]2-	µmol/kg	Fitzer, Susan C	Calculated using CO2SYS
27	Calcite saturation state	Omega Cal		Fitzer, Susan C	Calculated using CO2SYS
28	Aragonite saturation state	Omega Arg		Fitzer, Susan C	Calculated using CO2SYS
29	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
30	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
31	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
32	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
33	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
34	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
35	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
36	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
37	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

2516 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Species	2 Image no/name (shell)	3 Sample ID (shell)	4 pCO2water_SST_wet [µatm] (treatment)	5 Temp [°C] (treatment)	6 µ [mm/month] (shell)	7 µ [mm/month] (calcite)	8 µ [mm/month] (aragonite)	9 Date (analysed)	10 Sample ID (test)	11 CA activity [EU/ml] (eExtrapallial fluid)	12 Wet m [g] (mantle tissue)	13 CA act [EU/mg] (mantle tissue)	14 Sal	15 Sal std dev [±]	16 O2 sat [%]	17 O2 std dev [±]	18 Temp [°C]	19 Temp std dev [±]	20 pH (total scale, Calculated using...)	21 pCO2water_SST_wet [µatm]	22 pCO2 std dev [±]	23 AT [µmol/kg] (Potentiometric titration)	24 AT std dev [±] (Potentiometric titration)	25 [HCO3]- [µmol/kg] (Calculated using CO2SYS)	26 [CO3]2- [µmol/kg] (Calculated using CO2SYS)	27 Omega Cal (Calculated using CO2SYS)	28 Omega Arg (Calculated using CO2SYS)	29 CSC flag (Calculated using seacarb afte...)	30 pH (total scale, Calculated using...)	31 CO2 [µmol/kg] (Calculated using seacarb afte...)	32 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	33 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	34 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	35 DIC [µmol/kg] (Calculated using seacarb afte...)	36 Omega Arg (Calculated using seacarb afte...)	37 Omega Cal (Calculated using seacarb afte...)
Mytilus edulis (molllusk)	6	6	380	10	1.62	0.26	0.79						33	1.4	96	1.8	9.40	0.36	7.7	376	10	635	29	590	12	0.29	0.18	24	7.55	16.96	374.56	589.86	11.90	618.72	0.18	0.29
Mytilus edulis (molllusk)	14	7	380	10	0.81	0.30	0.40						33	1.4	96	1.8	9.40	0.36	7.7	376	10	635	29	590	12	0.29	0.18	24	7.55	16.96	374.56	589.86	11.90	618.72	0.18	0.29
Mytilus edulis (molllusk)	23	5	380	10	0.66	0.31	0.25						33	1.4	96	1.8	9.40	0.36	7.7	376	10	635	29	590	12	0.29	0.18	24	7.55	16.96	374.56	589.86	11.90	618.72	0.18	0.29
Mytilus edulis (molllusk)	24	8	380	10	1.35	0.25	0.35						33	1.4	96	1.8	9.40	0.36	7.7	376	10	635	29	590	12	0.29	0.18	24	7.55	16.96	374.56	589.86	11.90	618.72	0.18	0.29
Mytilus edulis (molllusk)	9	3	380	12	1.62	0.31	0.14						36	1.5	98	2.4	11.58	0.69	7.7	376	10	703	79	642	71	0.39	0.25	24	7.59	15.52	374.60	641.83	16.58	673.94	0.25	0.39
Mytilus edulis (molllusk)	16	4	380	12	1.19	0.27	0.90						36	1.5	98	2.4	11.58	0.69	7.7	376	10	703	79	642	71	0.39	0.25	24	7.59	15.52	374.60	641.83	16.58	673.94	0.25	0.39
Mytilus edulis (molllusk)	26	2	380	12	1.89	0.24	0.27						36	1.5	98	2.4	11.58	0.69	7.7	376	10	703	79	642	71	0.39	0.25	24	7.59	15.52	374.60	641.83	16.58	673.94	0.25	0.39
Mytilus edulis (molllusk)	28	1	380	12	1.03	0.31	0.36						36	1.5	98	2.4	11.58	0.69	7.7	376	10	703	79	642	71	0.39	0.25	24	7.59	15.52	374.60	641.83	16.58	673.94	0.25	0.39
Mytilus edulis (molllusk)	15	10	550	10	0.78	0.45	0.13						33	1.6	99	2.2	10.01	0.56	7.7	554	63	971	186	909	20	0.47	0.30	24	7.57	24.49	551.90	908.79	19.72	953.00	0.30	0.48
Mytilus edulis (molllusk)	19	9	550	10	1.00	0.27	0.19						33	1.6	99	2.2	10.01	0.56	7.7	554	63	971	186	909	20	0.47	0.30	24	7.57	24.49	551.90	908.79	19.72	953.00	0.30	0.48
Mytilus edulis (molllusk)	20	11	550	10	0.86	0.25	0.22						33	1.6	99	2.2	10.01	0.56	7.7	554	63	971	186	909	20	0.47	0.30	24	7.57	24.49	551.90	908.79	19.72	953.00	0.30	0.48
Mytilus edulis (molllusk)	22	12	550	10	0.32	0.24	0.21						33	1.6	99	2.2	10.01	0.56	7.7	554	63	971	186	909	20	0.47	0.30	24	7.57	24.49	551.90	908.79	19.72	953.00	0.30	0.48
Mytilus edulis (molllusk)	1	26	750	10	1.05	0.25	0.27						28	4.1	99	4.8	10.28	0.34	7.5	769	42	754	55	726	8	0.21	0.13	24	7.34	34.67	766.09	726.42	8.29	769.38	0.13	0.21
Mytilus edulis (molllusk)	2	28	750	10	0.37	0.26	0.33						28	4.1	99	4.8	10.28	0.34	7.5	769	42	754	55	726	8	0.21	0.13	24	7.34	34.67	766.09	726.42	8.29	769.38	0.13	0.21
Mytilus edulis (molllusk)	3	27	750	10	1.16	0.28	0.22						28	4.1	99	4.8	10.28	0.34	7.5	769	42	754	55	726	8	0.21	0.13	24	7.34	34.67	766.09	726.42	8.29	769.38	0.13	0.21
Mytilus edulis (molllusk)	4	25	750	10	0.49	0.23	0.24						28	4.1	99	4.8	10.28	0.34	7.5	769	42	754	55	726	8	0.21	0.13	24	7.34	34.67	766.09	726.42	8.29	769.38	0.13	0.21
Mytilus edulis (molllusk)	7	17	750	12	1.38	0.20	0.66						37	3.8	98	2.6	12.34	0.43	7.4	769	42	681	42	649	9	0.21	0.13	24	7.28	30.81	766.16	648.33	8.75	687.89	0.13	0.21
Mytilus edulis (molllusk)	8	18	750	12	1.16	0.32	0.53						37	3.8	98	2.6	12.34	0.43	7.4	769	42	681	42	649	9	0.21	0.13	24	7.28	30.81	766.16	648.33	8.75	687.89	0.13	0.21
Mytilus edulis (molllusk)	12	19	750	12	0.71	0.29	0.24						37	3.8	98	2.6	12.34	0.43	7.4	769	42	681	42	649	9	0.21	0.13	24	7.28	30.81	766.16	648.33	8.75	687.89	0.13	0.21
Mytilus edulis (molllusk)	21	20	750	12	0.90	0.49	0.17						37	3.8	98	2.6	12.34	0.43	7.4	769	42	681	42	649	9	0.21	0.13	24	7.28	30.81	766.16	648.33	8.75	687.89	0.13	0.21
Mytilus edulis (molllusk)	5	24	1000	10	0.87	0.41	0.27						34	4.6	99	2.0	10.23	0.40	7.3	1133	32	698	6	678	6	0.13	0.08	24	7.13	49.44	1128.71	677.99	5.54	732.96	0.08	0.13
Mytilus edulis (molllusk)	10	23	1000	10	0.83	0.34	0.14						34	4.6	99	2.0	10.23	0.40	7.3	1133	32	698	6	678	6	0.13	0.08	24	7.13	49.44	1128.71	677.99	5.54	732.96	0.08	0.13
Mytilus edulis (molllusk)	11	22	1000	10	1.51	0.41	0.14						34	4.6	99	2.0	10.23	0.40	7.3	1133	32	698	6	678	6	0.13	0.08	24	7.13	49.44	1128.71	677.99	5.54	732.96	0.08	0.13
Mytilus edulis (molllusk)	13	21	1000	10	1.69	0.32	0.38						34	4.6	99	2.0	10.23	0.40	7.3	1133	32	698	6	678	6	0.13	0.08	24	7.13	49.44	1128.71	677.99	5.54	732.96	0.08	0.13
Mytilus edulis (molllusk)	17	15	1000	12	0.95	0.40	0.19						37	2.1	98	2.0	12.04	0.35	7.2	1133	32	642	28	621	5	0.13	0.08	24	7.10	45.83	1128.81	621.42	5.38	672.64	0.08	0.13
Mytilus edulis (molllusk)	18	16	1000	12	0.73	0.38	0.36						37	2.1	98	2.0	12.04	0.35	7.2	1133	32	642	28	621	5	0.13	0.08	24	7.10	45.83	1128.81	621.42	5.38	672.64	0.08	0.13
Mytilus edulis (molllusk)	25	13	1000	12	0.49	0.22	0.13						37	2.1	98	2.0	12.04	0.35	7.2	1133	32	642	28	621	5	0.13	0.08	24	7.10	45.83	1128.81	621.42	5.38	672.64	0.08	0.13
Mytilus edulis (molllusk)	27	14	1000	12	0.87	0.41	0.21						37	2.1	98	2.0	12.04	0.35	7.2	1133	32	642	28	621	5	0.13	0.08	24	7.10	45.83	1128.81	621.42	5.38	672.64	0.08	0.13
Mytilus edulis (molllusk)			380	10				2013-10-10	6	4.285714286			33	1.4	96	1.8	9.40	0.36	7.7	376	10	635	29	590	12	0.29	0.18	24	7.55	16.96	374.56	589.86	11.90	618.72	0.18	0.29
Mytilus edulis (molllusk)			380	10				2013-10-07	2	2.860000000			33	1.4	96	1.8	9.40	0.36	7.7	376	10	635	29	590	12	0.29	0.18	24	7.55	16.96	374.56	589.86	11.90	618.72	0.18	0.29
Mytilus edulis (molllusk)			380	10				2013-10-07	4	24.060000000			33	1.4	96	1.8	9.40	0.36	7.7	376	10	635	29	590	12	0.29	0.18	24	7.55	16.96	374.56	589.86	11.90	618.72	0.18	0.29
Mytilus edulis (molllusk)			380	10				2013-10-07	10	7.140000000			33	1.4	96	1.8	9.40	0.36	7.7	376	10	635	29	590	12	0.29	0.18	24	7.55	16.96	374.56	589.86	11.90	618.72	0.18	0.29
Mytilus edulis (molllusk)			380	12				2013-10-15	6	4.571428571			36	1.5	98	2.4	11.58	0.69	7.7	376	10	703	79	642	71	0.39	0.25	24	7.59	15.52	374.60	641.83	16.58	673.94	0.25	0.39
Mytilus edulis (molllusk)			380	12				2013-10-15	10	0.000000000			36	1.5	98	2.4	11.58	0.69	7.7	376	10	703	79	642	71	0.39	0.25	24	7.59	15.52	374.60	641.83	16.58	673.94	0.25	0.39
Mytilus edulis (molllusk)			380	12				2013-10-10	2	0.000000000			36	1.5	98	2.4	11.58	0.69	7.7	376	10	703	79	642	71	0.39	0.25	24	7.59	15.52	374.60	641.83	16.58	673.94	0.25	0.39
Mytilus edulis (molllusk)			380	12				2013-10-10	4	0.000000000			36	1.5	98	2.4	11.58	0.69	7.7	376	10	703	79	642	71	0.39	0.25	24	7.59	15.52	374.60	641.83	16.58	673.94	0.25	0.39
Mytilus edulis (molllusk)			550	10				2013-10-10	8	-1.970443350			33	1.6	99	2.2	10.01	0.56	7.7	554	63	971	186	909	20	0.47	0.30	24	7.57	24.49	551.90	908.79	19.72	953.00	0.30	0.48
Mytilus edulis (molllusk)			550	10				2013-10-10	10	8.791208791			33	1.6	99	2.2	10.01	0.56	7.7	554	63	971	186	909	20	0.47	0.30	24	7.57	24.49	551.90	908.79	19.72	953.00	0.30	0.48
Mytilus edulis (molllusk)			550	10				2013-10-07	6	30.770000000			33	1.6	99	2.2	10.01	0.56	7.7	554	63	971	186	909	20	0.47	0.30	24	7.57	24.49	551.90	908.79	19.72	953.00	0.30	0.48
Mytilus edulis (molllusk)			550	10				2013-10-07	8	-8.570000000			33	1.6	99	2.2	10.01	0.56	7.7	554	63	971	186	909	20	0.47	0.30	24	7.57	24.49	551.90	908.79	19.72	953.00	0.30	0.48
Mytilus edulis (molllusk)			750	10				2013-10-15	2	3.986710963			28	4.1	99	4.8	10.28	0.34	7.5	769	42	754	55	726	8	0.21	0.13	24	7.34	34.67	766.09	726.42	8.29	769.38	0.13	0.21
Mytilus edulis (molllusk)			750	10				2013-10-14	8	2.597402597			28	4.1	99	4.8	10.28	0.34	7.5	769	42	754	55	726	8	0.21	0.13	24	7.34	34.67	766.09	726.42	8.29	769.38	0.13	0.21
Mytilus edulis (molllusk)			750	10				2013-10-14	10	7.792207792			28	4.1	99	4.8	10.28	0.34	7.5	769	42	754	55	726	8	0.21	0.13	24	7.34	34.67	766.09	726.42	8.29	769.38	0.13	0.21
Mytilus edulis (molllusk)			750	10				2013-10-09	6	2.380952381			28	4.1	99	4.8	10.28	0.34	7.5	769	42	754	55	726	8	0.21	0.13	24	7.34	34.67	766.09	726.42	8.29	769.38	0.13	0.21
Mytilus edulis (molllusk)			750	12				2013-10-15	4	13.533834590			37	3.8	98	2.6	12.34	0.43	7.4	769	42	681	42	649	9	0.21	0.13	24	7.28	30.81	766.16	648.33	8.75	687.89	0.13	0.21
Mytilus edulis (molllusk)			750	12				2013-10-14	4	10.000000000			37	3.8	98	2.6	12.34	0.43	7.4	769	42	681	42	649	9	0.21	0.13	24	7.28	30.81	766.16	648.33	8.75	687.89	0.13	0.21
Mytilus edulis (molllusk)			750	12				2013-10-09	2	14.285714290			37	3.8	98	2.6	12.34	0.43	7.4	769	42	681	42	649	9	0.21	0.13	24	7.28	30.81	766.16	648.33	8.75	687.89	0.13	0.21
Mytilus edulis (molllusk)			750	12				2013-10-09	4	-1.785714286			37	3.8	98	2.6	12.34	0.43	7.4	769	42	681	42	649	9	0.21	0.13	24	7.28	30.81	766.16	648.33	8.75	687.89	0.13	0.21
Mytilus edulis (molllusk)			1000	10				2013-10-14	2	2.857142857			34	4.6	99	2.0	10.23	0.40	7.3	1133	32	698	6	678	6	0.13	0.08	24	7.13	49.44	1128.71	677.99	5.54	732.96	0.08	0.13
Mytilus edulis (molllusk)			1000	10				2013-10-14	6	1.904761905			34	4.6	99	2.0	10.23	0.40	7.3	1133	32	698	6	678	6	0.13	0.08	24	7.13	49.44	1128.71	677.99	5.54	732.96	0.08	0.13
Mytilus edulis (molllusk)			1000	10				2013-10-08	2	-1.904761905			34	4.6	99	2.0	10.23	0.40	7.3	1133	32	698	6	678	6	0.13	0.08	24	7.13	49.44	1128.71	677.99	5.54	732.96	0.08	0.13
Mytilus edulis (molllusk)			1000	10				2013-10-08	6	6.015037594			34	4.6	99	2.0	10.23	0.40	7.3	1133	32	698	6	678	6	0.13	0.08	24	7.13	49.44	1128.71	677.99	5.54	732.96	0.08	0.13
Mytilus edulis (molllusk)			1000	12				2013-10-15	8	8.791208791			37	2.1	98	2.0	12.04	0.35	7.2	1133	32	642	28	621	5	0.13	0.08	24	7.10	45.83	1128.81	621.42	5.38	672.64	0.08	0.13
Mytilus edulis (molllusk)			1000	12				2013-10-10	12	3.174603175			37	2.1	98	2.0	12.04	0.35	7.2	1133	32	642	28	621	5	0.13	0.08	24	7.10	45.83	1128.81	621.42	5.38	672.64	0.08	0.13
Mytilus edulis (molllusk)			1000	12				2013-10-08	4	-3.361344538			37	2.1	98	2.0	12.04	0.35	7.2	1133	32	642	28	621	5	0.13	0.08	24	7.10	45.83	1128.81	621.42	5.38	672.64	0.08	0.13
Mytilus edulis (molllusk)			380	10					6		0.1105	-0.030	33	1.4	96	1.8	9.40	0.36	7.7	376	10	635	29	590	12	0.29	0.18	24	7.55	16.96	374.56	589.86	11.90	618.72	0.18	0.29
Mytilus edulis (molllusk)			380	10					2		0.0518	0.840	33	1.4	96	1.8	9.40	0.36	7.7	376	10	635	29	590	12	0.29	0.18	24	7.55	16.96	374.56	589.86	11.90	618.72	0.18	0.29
Mytilus edulis (molllusk)			380	10					4		0.1135	0.108	33	1.4	96	1.8	9.40	0.36	7.7	376	10	635	29	590	12	0.29	0.18	24	7.55	16.96	374.56	589.86	11.90	618.72	0.18	0.29
Mytilus edulis (molllusk)			380	10					10		0.0159	2.960	33	1.4	96	1.8	9.40	0.36	7.7	376	10	635	29	590	12	0.29	0.18	24	7.55	16.96	374.56	589.86	11.90	618.72	0.18	0.29
Mytilus edulis (molllusk)			380	12					6		0.1516	-0.219	36	1.5	98	2.4	11.58	0.69	7.7	376	10	703	79	642	71	0.39	0.25	24	7.59	15.52	374.60	641.83	16.58	673.94	0.25	0.39
Mytilus edulis (molllusk)			380	12					10		0.1284	0.414	36	1.5	98	2.4	11.58	0.69	7.7	376	10	703	79	642	71	0.39	0.25	24	7.59	15.52	374.60	641.83	16.58	673.94	0.25	0.39
Mytilus edulis (molllusk)			380	12					2		0.1449	0.217	36	1.5	98	2.4	11.58	0.69	7.7	376	10	703	79	642	71	0.39	0.25	24	7.59	15.52	374.60	641.83	16.58	673.94	0.25	0.39
Mytilus edulis (molllusk)			380	12					4		0.1754	0.081	36	1.5	98	2.4	11.58	0.69	7.7	376	10	703	79	642	71	0.39	0.25	24	7.59	15.52	374.60	641.83	16.58	673.94	0.25	0.39
Mytilus edulis (molllusk)			550	10					8		0.0488	0.323	33	1.6	99	2.2	10.01	0.56	7.7	554	63	971	186	909	20	0.47	0.30	24	7.57	24.49	551.90	908.79	19.72	953.00	0.30	0.48
Mytilus edulis (molllusk)			550	10					10		0.0346	1.143	33	1.6	99	2.2	10.01	0.56	7.7	554	63	971	186	909	20	0.47	0.30	24	7.57	24.49	551.90	908.79	19.72	953.00	0.30	0.48
Mytilus edulis (molllusk)			550	10					6		0.0951	-0.060	33	1.6	99	2.2	10.01	0.56	7.7	554	63	971	186	909	20	0.47	0.30	24	7.57	24.49	551.90	908.79	19.72	953.00	0.30	0.48
Mytilus edulis (molllusk)			550	10					8		0.0763	0.200	33	1.6	99	2.2	10.01	0.56	7.7	554	63	971	186	909	20	0.47	0.30	24	7.57	24.49	551.90	908.79	19.72	953.00	0.30	0.48
Mytilus edulis (molllusk)			750	10					2		0.1429	0.074	28	4.1	99	4.8	10.28	0.34	7.5	769	42	754	55	726	8	0.21	0.13	24	7.34	34.67	766.09	726.42	8.29	769.38	0.13	0.21
Mytilus edulis (molllusk)			750	10					8		0.1340	-0.109	28	4.1	99	4.8	10.28	0.34	7.5	769	42	754	55	726	8	0.21	0.13	24	7.34	34.67	766.09	726.42	8.29	769.38	0.13	0.21
Mytilus edulis (molllusk)			750	10					10		0.0474	0.336	28	4.1	99	4.8	10.28	0.34	7.5	769	42	754	55	726	8	0.21	0.13	24	7.34	34.67	766.09	726.42	8.29	769.38	0.13	0.21
Mytilus edulis (molllusk)			750	10					6		0.1349	0.212	28	4.1	99	4.8	10.28	0.34	7.5	769	42	754	55	726	8	0.21	0.13	24	7.34	34.67	766.09	726.42	8.29	769.38	0.13	0.21
Mytilus edulis (molllusk)			750	12					4		0.2208	0.144	37	3.8	98	2.6	12.34	0.43	7.4	769	42	681	42	649	9	0.21	0.13	24	7.28	30.81	766.16	648.33	8.75	687.89	0.13	0.21
Mytilus edulis (molllusk)			750	12					4		0.0775	0.233	37	3.8	98	2.6	12.34	0.43	7.4	769	42	681	42	649	9	0.21	0.13	24	7.28	30.81	766.16	648.33	8.75	687.89	0.13	0.21
Mytilus edulis (molllusk)			750	12					2		0.1287	-0.127	37	3.8	98	2.6	12.34	0.43	7.4	769	42	681	42	649	9	0.21	0.13	24	7.28	30.81	766.16	648.33	8.75	687.89	0.13	0.21
Mytilus edulis (molllusk)			750	12					4		0.1427	0.032	37	3.8	98	2.6	12.34	0.43	7.4	769	42	681	42	649	9	0.21	0.13	24	7.28	30.81	766.16	648.33	8.75	687.89	0.13	0.21
Mytilus edulis (molllusk)			1000	10					2		0.1969	0.224	34	4.6	99	2.0	10.23	0.40	7.3	1133	32	698	6	678	6	0.13	0.08	24	7.13	49.44	1128.71	677.99	5.54	732.96	0.08	0.13
Mytilus edulis (molllusk)			1000	10					6		0.0219	1.472	34	4.6	99	2.0	10.23	0.40	7.3	1133	32	698	6	678	6	0.13	0.08	24	7.13	49.44	1128.71	677.99	5.54	732.96	0.08	0.13
Mytilus edulis (molllusk)			1000	10					2		0.0559	0.175	34	4.6	99	2.0	10.23	0.40	7.3	1133	32	698	6	678	6	0.13	0.08	24	7.13	49.44	1128.71	677.99	5.54	732.96	0.08	0.13
Mytilus edulis (molllusk)			1000	10					6		0.0909	-0.039	34	4.6	99	2.0	10.23	0.40	7.3	1133	32	698	6	678	6	0.13	0.08	24	7.13	49.44	1128.71	677.99	5.54	732.96	0.08	0.13
Mytilus edulis (molllusk)			1000	12					8		0.1281	0.320	37	2.1	98	2.0	12.04	0.35	7.2	1133	32	642	28	621	5	0.13	0.08	24	7.10	45.83	1128.81	621.42	5.38	672.64	0.08	0.13
Mytilus edulis (molllusk)			1000	12					12		0.0411	0.363	37	2.1	98	2.0	12.04	0.35	7.2	1133	32	642	28	621	5	0.13	0.08	24	7.10	45.83	1128.81	621.42	5.38	672.64	0.08	0.13
Mytilus edulis (molllusk)			1000	12					4		0.0318	0.317	37	2.1	98	2.0	12.04	0.35	7.2	1133	32	642	28	621	5	0.13	0.08	24	7.10	45.83	1128.81	621.42	5.38	672.64	0.08	0.13