Citation:

es501611u

Name: Interactive effects of ocean acidification, elevated temperature, and reduced salinity on early-life stages of the Pacific oyster
Published: 2014-10-22
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Animalia; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Crassostrea gigas; Development; Growth/Morphology; Laboratory experiment; Mollusca; North Pacific; Pelagos; Salinity; Single species; Temperate; Temperature; Zooplankton

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Abstract:

Ocean acidification (OA) effects on larvae are partially attributed for the rapidly declining oyster production in the Pacific Northwest region of the United States. This OA effect is a serious concern in SE Asia, which produces >80% of the world's oysters. Because climate-related stressors rarely act alone, we need to consider OA effects on oysters in combination with warming and reduced salinity. Here, the interactive effects of these three climate-related stressors on the larval growth of the Pacific oyster, Crassostrea gigas, were examined. Larvae were cultured in combinations of temperature (24 and 30 °C), pH (8.1 and 7.4), and salinity (15 psu and 25 psu) for 58 days to the early juvenile stage. Decreased pH (pH 7.4), elevated temperature (30 °C), and reduced salinity (15 psu) significantly delayed pre- and post-settlement growth. Elevated temperature lowered the larval lipid index, a proxy for physiological quality, and negated the negative effects of decreased pH on attachment and metamorphosis only in a salinity of 25 psu. The negative effects of multiple stressors on larval metamorphosis were not due to reduced size or depleted lipid reserves at the time of metamorphosis. Our results supported the hypothesis that the C. gigas larvae are vulnerable to the interactions of OA with reduced salinity and warming in Yellow Sea coastal waters now and in the future.

Keyword(s):

Animalia; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Crassostrea gigas; Development; Growth/Morphology; Laboratory experiment; Mollusca; North Pacific; Pelagos; Salinity; Single species; Temperate; Temperature; Zooplankton

Further details:

Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: 36.066670 * Longitude: 120.366670

Date/Time Start: 2012-07-01T00:00:00 * Date/Time End: 2012-07-30T00:00:00

Event(s):

Tsingdao * Latitude: 36.066670 * Longitude: 120.366670 * Date/Time Start: 2012-07-01T00:00:00 * Date/Time End: 2012-07-30T00:00:00 * Method/Device: Experiment (EXP) * Comment: Adult Pacific oysters (Crassostrea gigas) were collected from the coastal area of Tsingdao (China) and transported in emersed condition to
Hong Kong by air via Guangzhou (China).

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-10-21.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Species	Species		Thiyagarajan, Vengatesen
2	Treatment	Treat		Thiyagarajan, Vengatesen		C=control, P=decreased pH, S=reduced salinity, T=elevated temperature
3	Replicate	Repl		Thiyagarajan, Vengatesen
4	pH	pH		Thiyagarajan, Vengatesen		average over the whole larval culture period, NBS scale
5	Temperature, water	Temp	°C	Thiyagarajan, Vengatesen		average over the whole larval culture period
6	Salinity	Sal		Thiyagarajan, Vengatesen		average over the whole larval culture period
7	pH	pH		Thiyagarajan, Vengatesen		average over the whole juvenile culture period, NBS scale
8	Temperature, water	Temp	°C	Thiyagarajan, Vengatesen		average over the whole juvenile culture period
9	Salinity	Sal		Thiyagarajan, Vengatesen		average over the whole juvenile culture period
10	Settlement	Settlem	%	Thiyagarajan, Vengatesen
11	Larvae	Larvae	%	Thiyagarajan, Vengatesen		day 15 developmental stage: D shaped
12	Larvae	Larvae	%	Thiyagarajan, Vengatesen		day 15 developmental stage: early umbo
13	Larvae	Larvae	%	Thiyagarajan, Vengatesen		day 15 developmental stage: umbo
14	Larvae	Larvae	%	Thiyagarajan, Vengatesen		day 15 developmental stage: pedivelieger
15	Growth rate	µ	mm²/day	Thiyagarajan, Vengatesen		day 1-10, larval
16	Growth rate	µ	mm²/day	Thiyagarajan, Vengatesen		day 1-15, larval
17	Growth rate	µ	mm²/day	Thiyagarajan, Vengatesen		day 10-15, larval
18	Growth rate	µ	mm²/day	Thiyagarajan, Vengatesen		juvenile
19	Lipid index	Lipid index		Thiyagarajan, Vengatesen
20	pH	pH		Thiyagarajan, Vengatesen		larval, NBS scale
21	Temperature, water	Temp	°C	Thiyagarajan, Vengatesen		larval
22	Alkalinity, total	AT	µmol/kg	Thiyagarajan, Vengatesen		larval
23	Salinity	Sal		Thiyagarajan, Vengatesen		larval
24	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Thiyagarajan, Vengatesen		larval
25	Carbonate ion	[CO3]2-	µmol/kg	Thiyagarajan, Vengatesen		larval
26	Calcite saturation state	Omega Cal		Thiyagarajan, Vengatesen		larval
27	Aragonite saturation state	Omega Arg		Thiyagarajan, Vengatesen		larval
28	pH	pH		Thiyagarajan, Vengatesen		juvenile, NBS scale
29	Temperature, water	Temp	°C	Thiyagarajan, Vengatesen		juvenile
30	Alkalinity, total	AT	µmol/kg	Thiyagarajan, Vengatesen		juvenile
31	Salinity	Sal		Thiyagarajan, Vengatesen		juvenile
32	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Thiyagarajan, Vengatesen		juvenile
33	Carbonate ion	[CO3]2-	µmol/kg	Thiyagarajan, Vengatesen		juvenile
34	Calcite saturation state	Omega Cal		Thiyagarajan, Vengatesen		juvenile
35	Aragonite saturation state	Omega Arg		Thiyagarajan, Vengatesen		juvenile
36	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
37	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	larval, total scale
38	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	larval
39	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	larval
40	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	larval
41	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	larval
42	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	larval
43	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	larval
44	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	larval
45	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	larval
46	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	juvenile, total scale
47	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	juvenile
48	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	juvenile
49	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	juvenile
50	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	juvenile
51	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	juvenile
52	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	juvenile
53	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	juvenile
54	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	juvenile

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1563 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Species	2 Treat (C=control, P=decreased pH, S=...)	3 Repl	4 pH (average over the whole larval...)	5 Temp [°C] (average over the whole larval...)	6 Sal (average over the whole larval...)	7 pH (average over the whole juveni...)	8 Temp [°C] (average over the whole juveni...)	9 Sal (average over the whole juveni...)	10 Settlem [%]	11 Larvae [%] (day 15 developmental stage: D...)	12 Larvae [%] (day 15 developmental stage: e...)	13 Larvae [%] (day 15 developmental stage: umbo)	14 Larvae [%] (day 15 developmental stage: p...)	15 µ [mm²/day] (day 1-10, larval)	16 µ [mm²/day] (day 1-15, larval)	17 µ [mm²/day] (day 10-15, larval)	18 µ [mm²/day] (juvenile)	19 Lipid index	20 pH (larval, NBS scale)	21 Temp [°C] (larval)	22 AT [µmol/kg] (larval)	23 Sal (larval)	24 pCO2water_SST_wet [µatm] (larval)	25 [CO3]2- [µmol/kg] (larval)	26 Omega Cal (larval)	27 Omega Arg (larval)	28 pH (juvenile, NBS scale)	29 Temp [°C] (juvenile)	30 AT [µmol/kg] (juvenile)	31 Sal (juvenile)	32 pCO2water_SST_wet [µatm] (juvenile)	33 [CO3]2- [µmol/kg] (juvenile)	34 Omega Cal (juvenile)	35 Omega Arg (juvenile)	36 CSC flag	37 pH (larval, total scale)	38 CO2 [µmol/kg] (larval)	39 pCO2water_SST_wet [µatm] (larval)	40 fCO2water_SST_wet [µatm] (larval)	41 [HCO3]- [µmol/kg] (larval)	42 [CO3]2- [µmol/kg] (larval)	43 DIC [µmol/kg] (larval)	44 Omega Arg (larval)	45 Omega Cal (larval)	46 pH (juvenile, total scale)	47 CO2 [µmol/kg] (juvenile)	48 pCO2water_SST_wet [µatm] (juvenile)	49 fCO2water_SST_wet [µatm] (juvenile)	50 [HCO3]- [µmol/kg] (juvenile)	51 [CO3]2- [µmol/kg] (juvenile)	52 DIC [µmol/kg] (juvenile)	53 Omega Arg (juvenile)	54 Omega Cal (juvenile)
Crassostrea gigas (mollusk)	Control	1	8.13	23.70	25	8.15	24.635	25	33.33	1.02	8.16	90.82	0.000	0.00000504	0.00000868	0.00000060	0.00235	0.0993	8.03	21.9	1850.6	25.0	538.176	93.974	2.421	1.532									26	7.89	17.38	537.27	535.51	1619.95	93.95	1731.28	1.53	2.42
Crassostrea gigas (mollusk)	Control	2	8.13	23.74	25	8.15	24.660	25	28.22	0.00	5.62	94.38	0.000	0.00000442	0.00001053	0.00000113	0.01275	0.1182	8.04	22.1	1790.7	25.0	507.683	93.239	2.404	1.522	8.07	23.8	1587.4	24	429.51	88.91	2.32	1.47	26	7.90	16.31	506.83	505.18	1560.43	93.22	1669.96	1.52	2.40	7.92	13.25	428.40	427.03	1363.99	88.93	1466.17	1.47	2.32
Crassostrea gigas (mollusk)	Control	3	8.14	23.76	25	8.16	24.630	25	18.64	0.00	1.32	98.68	0.000	0.00000381	0.00000924	0.00000113	0.01119	0.0909	8.03	22.0	1909.4	25.0	556.054	97.310	2.508	1.587	8.07	23.9	1636.2	24	439.33	92.42	2.41	1.53	26	7.89	17.91	555.12	553.31	1671.97	97.29	1787.17	1.59	2.51	7.92	13.55	439.39	437.99	1405.42	92.45	1511.42	1.53	2.41
Crassostrea gigas (mollusk)	Control	4	8.13	23.61	25	8.16	24.560	25	30.77	0.79	0.79	97.62	0.000	0.00000537	0.00001112	0.00000090	0.00397	0.0909	8.06	22.0	1767.9	25.0	475.315	95.505	2.461	1.558	8.06	23.8	1600.5	24	440.81	88.49	2.31	1.46	26	7.92	15.31	474.50	472.95	1531.42	95.48	1642.21	1.56	2.46	7.92	13.59	439.70	438.30	1378.55	88.51	1480.65	1.46	2.31
Crassostrea gigas (mollusk)	S	1	8.08	23.73	25	8.09	24.600	15	28.91	0.00	2.48	97.52	0.000	0.00000446	0.00000949	0.00000092	0.02357	0.0822	7.97	22.2	1491.5	18.0	564.179	55.246	1.501	0.914	8.03	23.8	1084.5	15	371.57	43.69	1.23	0.74	26	7.82	18.78	563.86	562.02	1356.55	55.29	1430.62	0.91	1.50	7.88	12.03	371.15	369.97	974.14	43.76	1029.93	0.74	1.23
Crassostrea gigas (mollusk)	S	2	8.09	23.75	25	8.11	24.650	15	52.55	2.14	4.29	90.71	0.710	0.00000419	0.00000892	0.00000091	0.00610	0.1130	7.96	22.4	1427.6	18.5	549.402	52.844	1.432	0.875	8.03	23.9	1096.3	15	378.98	43.91	1.23	0.74	26	7.81	18.14	549.03	547.24	1296.95	52.88	1367.97	0.88	1.43	7.88	12.27	379.60	378.39	985.61	43.99	1041.88	0.74	1.24
Crassostrea gigas (mollusk)	S	3	8.08	23.75	25	8.10	24.590	15	25.50	0.00	3.15	95.05	0.900	0.00000349	0.00001065	0.00000159	0.00000	0.0774	7.97	22.0	1388.0	19.5	481.124	53.155	1.388	0.860	8.03	23.8	1090.0	15	373.50	43.91	1.23	0.74	26	7.82	16.94	509.84	508.18	1254.22	53.39	1324.55	0.88	1.43	7.88	12.09	373.10	371.91	979.19	43.99	1035.27	0.74	1.24
Crassostrea gigas (mollusk)	S	4	8.07	23.72	15	8.09	24.660	15	45.57	0.96	4.33	90.87	1.440	0.00000386	0.00000932	0.00000112	0.01901	0.1365	7.97	22.0	1424.3	17.5	542.255	51.611	1.407	0.853	8.03	23.8	1067.2	15	365.52	42.97	1.21	0.72	26	7.82	18.20	541.93	540.16	1297.63	51.65	1367.48	0.85	1.41	7.88	11.84	365.12	363.95	958.27	43.05	1013.15	0.72	1.21
Crassostrea gigas (mollusk)	P	1	7.46	23.71	25	7.44	24.540	25	29.89	0.00	3.54	93.94	1.010	0.00000591	0.00000716	0.00000018	0.04912	0.1122	7.48	22.0	1882.3	24.0	2161.011	28.824	0.748	0.471	7.49	23.9	1612.4	24	1851.51	26.54	0.69	0.44	26	7.34	70.04	2159.00	2151.95	1812.23	28.80	1911.07	0.47	0.75	7.34	57.10	1851.78	1845.87	1546.04	26.55	1629.69	0.44	0.69
Crassostrea gigas (mollusk)	P	2	7.47	23.73	25	7.43	24.610	25	16.13	0.00	6.31	87.39	1.800	0.00000697	0.00000816	0.00000015	0.02968		7.46	22.2		24.0					7.49	23.9	1613.3	24	1837.81	26.75	0.70	0.44	26										7.34	56.68	1838.13	1832.26	1546.42	26.76	1629.86	0.44	0.70
Crassostrea gigas (mollusk)	P	3	7.41	23.73	25	7.42	24.620	25	19.59	0.00	5.49	90.11	1.100	0.00000705	0.00000770	0.00000008		0.0672	7.39	22.3	1811.7	25.0	2553.881	23.611	0.609	0.386	7.47	23.9	1558.2	24	1861.65	24.71	0.65	0.41	26	7.25	81.66	2551.51	2543.21	1753.64	23.59	1858.90	0.39	0.61	7.32	57.42	1862.02	1856.08	1495.98	24.72	1578.12	0.41	0.65
Crassostrea gigas (mollusk)	P	4	7.44	23.69	25	7.43	24.620	25	11.88	0.00	4.96	93.39	0.000	0.00000530	0.00000676	0.00000023	0.07748	0.0888	7.54	22.0	1816.2	25.0	1783.903	32.690	0.842	0.533	7.47	23.9	1552.4	24	1839.91	24.80	0.65	0.41	26	7.40	57.50	1781.91	1776.10	1735.77	32.66	1825.93	0.53	0.84	7.33	56.75	1840.19	1834.32	1489.90	24.81	1571.46	0.41	0.65
Crassostrea gigas (mollusk)	SP	1	7.43	23.73	15	7.39	24.580	15	34.62	0.00	3.08	93.85	0.000	0.00000386	0.00000783	0.00000082	0.06952	0.0778	7.34	21.8	1968.1	18.5	3378.125	18.443	0.498	0.304	7.38	23.8	1598.7	15	2681.80	15.56	0.44	0.26	26	7.19	113.41	3376.59	3365.54	1924.95	18.45	2056.81	0.30	0.50	7.23	86.83	2678.68	2670.12	1562.08	15.59	1664.50	0.26	0.44
Crassostrea gigas (mollusk)	SP	2	7.41	23.65	15	7.38	24.530	15	10.96	0.00	6.49	91.56	0.000	0.00000425	0.00000636	0.00000040	0.04659	0.0932	7.37	22.0	1368.5	19.0	2174.082	14.014	0.377	0.231	7.40	23.9	1099.2	15	1770.55	11.10	0.31	0.19	26	7.22	72.38	2172.60	2165.51	1333.47	14.02	1419.87	0.23	0.38	7.24	57.28	1771.65	1766.00	1071.27	11.14	1139.68	0.19	0.31
Crassostrea gigas (mollusk)	SP	3	7.40	23.76	15	7.37	24.570	15	17.14	0.00	6.92	93.08	0.000	0.00000399	0.00000629	0.00000046	0.04492	0.0990	7.35	22.0	1523.1	19.0	2537.938	14.920	0.402	0.246	7.41	23.9	1099.5	15	1702.66	11.53	0.32	0.19	26	7.20	84.50	2536.42	2528.13	1486.57	14.93	1586.00	0.25	0.40	7.26	55.07	1703.60	1698.16	1070.49	11.56	1137.13	0.19	0.33
Crassostrea gigas (mollusk)	SP	4	7.44	24.22	15	7.37	24.580	15	18.52	0.00	3.85	94.87	0.000	0.00000391	0.00000653	0.00000053	0.03740	0.0611	7.42	21.8	1447.4	19.0	2039.735	16.477	0.443	0.271	7.40	23.8	1074.2	15	1729.53	10.84	0.30	0.18	26	7.27	68.28	2038.31	2031.63	1406.64	16.48	1491.40	0.27	0.44	7.25	55.98	1727.06	1721.54	1046.83	10.86	1113.67	0.18	0.31
Crassostrea gigas (mollusk)	T	1	8.15	29.60	25	8.17	30.030	25		0.00	6.67	47.78	43.330	0.00000577	0.00001031	0.00000067			8.04	30.2	1856.3	25.0	552.643	120.568	3.199	2.073	8.12	29.7	1659.7	24	398.68	121.39	3.24	2.09	26	7.88	14.53	553.44	551.81	1561.64	120.81	1696.98	2.08	3.20	7.97	10.66	399.15	397.96	1358.00	121.63	1490.29	2.09	3.25
Crassostrea gigas (mollusk)	T	2	8.14	29.55	25	8.16	30.000	25	61.57	0.00	0.00	60.66	34.430	0.00000870	0.00001579	0.00000073	0.01101	0.0462	8.08	30.1	1817.1	25.0	485.342	127.040	3.369	2.183	8.12	29.9	2015.4	24	487.71	149.12	3.99	2.57	26	7.92	12.79	486.02	484.58	1505.28	127.29	1645.36	2.19	3.37	7.97	12.98	488.49	487.04	1657.94	149.38	1820.30	2.57	3.99
Crassostrea gigas (mollusk)	T	3	8.13	29.53	25	8.17	29.860	25	37.31	0.00	1.00	55.00	43.000	0.00001229	0.00001512	0.00000021	0.01143	0.0532	8.04	30.0	1816.3	25.0	540.055	117.340	3.111	2.015	8.13	30.0	1321.5	24	309.11	97.59	2.61	1.68	26	7.88	14.26	540.51	538.91	1528.48	117.52	1660.25	2.02	3.11	7.97	8.21	309.80	308.88	1066.26	97.83	1172.30	1.69	2.61
Crassostrea gigas (mollusk)	T	4	8.13	29.54	25	8.17	30.020	25	49.38	0.00	1.53	58.02	37.400	0.00000954	0.00001639	0.00000065	0.00397	0.0809	8.04	30.2	1818.4	24.0	549.546	115.183	3.083	1.988	8.13	30.2	1659.4	24	388.68	125.12	3.35	2.16	26	7.88	14.52	550.39	548.76	1537.32	115.43	1667.27	1.99	3.09	7.98	10.28	389.74	388.59	1348.37	125.40	1484.05	2.16	3.35
Crassostrea gigas (mollusk)	TS	1	8.12	29.59	15	8.14	29.950	15	35.48	0.00	6.47	64.75	20.860	0.00000640	0.00000984	0.00000047	0.01822	0.0911	8.02	30.2	1317.9	15.0	488.946	62.294	1.819	1.108	8.05	29.7	1080.9	15	371.62	52.99	1.54	0.94	26	7.86	13.53	490.46	489.01	1166.65	62.52	1242.70	1.11	1.82	7.89	10.39	372.06	370.95	947.17	53.19	1010.74	0.94	1.55
Crassostrea gigas (mollusk)	TS	2	8.12	29.59	15	8.13	30.050	15	26.88	0.00	3.68	60.74	25.770	0.00000738	0.00001284	0.00000065	0.01401	0.1291	8.02	30.2	1475.5	15.0	548.610	69.895	2.041	1.244	8.04	30.0	1094.5	15	380.21	53.66	1.56	0.95	26	7.86	15.19	550.46	548.84	1309.04	70.13	1394.35	1.25	2.05	7.88	10.58	381.71	380.58	959.42	53.87	1023.87	0.96	1.57
Crassostrea gigas (mollusk)	TS	3	8.13	29.61	15	8.14	30.030	15	48.70	0.00	2.05	56.85	30.820	0.00000687	0.00001198	0.00000065	0.01231	0.0506	8.01	30.2	1307.8	15.0	497.723	60.558	1.768	1.078	8.04	29.6	1229.1	15	427.22	59.82	1.74	1.06	26	7.85	13.77	499.27	497.79	1160.57	60.78	1235.12	1.08	1.77	7.88	11.99	428.37	427.10	1081.98	60.02	1153.99	1.06	1.75
Crassostrea gigas (mollusk)	TS	4	8.12	29.61	15				36.84	0.00	17.50	65.00	5.000	0.00000559	0.00001032	0.00000072		0.0749	8.02	30.2	1431.4	15.0	531.916	67.768	1.979	1.206	8.05	29.6	1083.4	15	372.14	52.87	1.54	0.94	26	7.86	14.72	533.64	532.07	1269.19	68.00	1351.91	1.21	1.98	7.88	10.45	373.52	372.41	950.02	53.07	1013.54	0.94	1.54
Crassostrea gigas (mollusk)	TP	1	7.60	29.62	25	7.49	30.070	25		0.00	0.00	85.71	14.290	0.00000553	0.00001507	0.00000146	0.04308		7.65	30.0	1902.0	25.0	1532.407	55.256	1.465	0.949	7.51	29.6	1631.8	24	1849.39	33.88	0.90	0.58	26	7.49	40.45	1533.63	1529.08	1767.74	55.40	1863.59	0.95	1.47	7.36	49.44	1847.82	1842.32	1547.56	33.98	1630.98	0.58	0.91
Crassostrea gigas (mollusk)	TP	2	7.58	29.57	25	7.48	30.080	25	23.26	0.00	0.00	52.78	44.440	0.00000858	0.00001252	0.00000041	0.02353	0.0673	7.65	30.0	1830.2	25.0	1473.833	53.144	1.409	0.913	7.52	29.9	1626.3	24	1832.94	34.31	0.92	0.59	26	7.49	38.90	1474.85	1470.48	1700.16	53.29	1792.35	0.91	1.41	7.36	48.67	1831.65	1826.21	1540.94	34.41	1624.02	0.59	0.92
Crassostrea gigas (mollusk)	TP	3	7.55	29.52	25	7.48	29.900	25	18.18	0.00	0.00	33.33	52.380	0.00000617	0.00001533	0.00000128	0.04950	0.0606	7.59	30.1	1812.7	25.0	1692.726	46.396	1.231	0.797	7.52	29.7	2134.5	24	2388.18	45.21	1.21	0.78	26	7.43	44.57	1693.70	1688.69	1698.99	46.53	1790.09	0.80	1.23	7.36	63.72	2386.83	2379.73	2027.27	45.33	2136.32	0.78	1.21
Crassostrea gigas (mollusk)	TP	4	7.51	29.62	25					0.00	8.57	80.95	6.670	0.00000967	0.00001284	0.00000030
Crassostrea gigas (mollusk)	TSP	1	7.46	29.61	15					5.45	32.73	47.27	5.450	0.00000411	0.00000720	0.00000060
Crassostrea gigas (mollusk)	TSP	2	7.46	29.52	15	7.45	30.070	15	13.75	2.74	30.14	56.16	2.740	0.00000818	0.00000980	0.00000018	0.00093	0.0955	7.46	29.8	1680.3	15.0	2463.022	23.598	0.687	0.418	7.49	30.3	1139.5	15	1570.22	17.18	0.50	0.31	26	7.30	68.66	2465.12	2457.79	1625.15	23.73	1717.54	0.42	0.69	7.32	43.29	1572.98	1568.34	1096.63	17.30	1157.22	0.31	0.51
Crassostrea gigas (mollusk)	TSP	3	7.46	29.44	15	7.45	30.000	15	13.60	0.00	23.53	51.47	19.120	0.00000947	0.00001154	0.00000020	0.00000	0.0755	7.50	29.0	1730.7	14.0	2328.835	25.072	0.734	0.443	7.46	29.9	1138.8	15	1667.53	16.02	0.47	0.28	26	7.34	66.52	2330.95	2323.96	1672.76	25.20	1764.49	0.45	0.74	7.30	46.31	1666.74	1661.79	1098.85	16.12	1161.28	0.29	0.47
Crassostrea gigas (mollusk)	TSP	4	7.45	29.47	15					0.00	14.52	22.58	0.000	0.00000684	0.00000396	-0.00000037