Citation:

j.jembe.2014.04.007

Name: Impact of elevated pCO2 on acid-base regulation of the sea urchin Echinometra mathaei and its relation to resistance to ocean acidification: A study in mesocosms
Published: 2014-09-19
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Acid-base regulation; Animalia; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Echinodermata; Echinometra mathaei; Growth/Morphology; Indian Ocean; Laboratory experiment; Respiration; Single species; Tropical

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Abstract:

Due to their low metabolism and apparent poor ion regulation ability, sea urchins could be particularly sensitive to ocean acidification resulting from increased dissolution of atmospheric carbon dioxide. Therefore, we evaluated the acid-base regulation ability of the coral reef sea urchin Echinometra mathaei and the impact of decreased pH on its growth and respiration activity. The study was conducted in two identical artificial reef mesocosms during seven weeks. Experimental tanks were maintained respectively at mean pHT 7.7 and 8.05 (with field-like night and day variations). The major physico-chemical parameters were identical, only pCO2 and pHT differed. Results indicate that E. mathaei can regulate the pH of its coelomic fluid in the considered range of pH, allowing a sustainable growth and ensuring an unaffected respiratory metabolism, at least at short term.

Keyword(s):

Acid-base regulation; Animalia; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Echinodermata; Echinometra mathaei; Growth/Morphology; Indian Ocean; Laboratory experiment; Respiration; Single species; Tropical

Further details:

Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-09-16.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Figure	Fig		Moulin, Laure
2	Species	Species		Moulin, Laure
3	Identification	ID		Moulin, Laure		mesocosm
4	pH	pH		Moulin, Laure	Potentiometric	seawater, total scale
5	Aquarium number	Aquarium		Moulin, Laure
6	Individual code	Individual code		Moulin, Laure
7	Growth	Growth	%	Moulin, Laure		as percentage of the initial skeletonweight
8	Respiration rate, oxygen	Resp O2	µmol/g/h	Moulin, Laure		per wet weight
9	Identification	ID		Moulin, Laure		respirometer
10	Date	Date		Moulin, Laure
11	Incubation duration	Inc dur	days	Moulin, Laure
12	Coelomic fluid, pH	pH (cf)		Moulin, Laure	Potentiometric	total scale
13	Coelomic fluid, alkalinity	AT (cf)	µmol/kg	Moulin, Laure	Potentiometric titration
14	Coelomic fluid, partial pressure of carbon dioxide	pCO2 (cf)	µatm	Moulin, Laure	Infrared spectrometric
15	Alkalinity, total	AT	µmol/kg	Moulin, Laure	Potentiometric titration	seawater
16	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Moulin, Laure	Calculated using seacarb	seawater
17	Salinity	Sal		Moulin, Laure		seawater
18	Temperature, water	Temp	°C	Moulin, Laure		seawater
19	Coelomic fluid, alkalinity	AT (cf)	µmol/kg	Moulin, Laure	Calculated
20	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
21	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
22	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
23	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
24	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
25	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
26	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
27	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
28	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

3152 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Fig	2 Species	3 ID (mesocosm)	4 pH (seawater, total scale, Potent...)	5 Aquarium	6 Individual code	7 Growth [%] (as percentage of the initial ...)	8 Resp O2 [µmol/g/h] (per wet weight)	9 ID (respirometer)	10 Date	11 Inc dur [days]	12 pH (cf) (total scale, Potentiometric)	13 AT (cf) [µmol/kg] (Potentiometric titration)	14 pCO2 (cf) [µatm] (Infrared spectrometric)	15 AT [µmol/kg] (seawater, Potentiometric titr...)	16 pCO2water_SST_wet [µatm] (seawater, Calculated using se...)	17 Sal (seawater)	18 Temp [°C] (seawater)	19 AT (cf) [µmol/kg] (Calculated)	20 CSC flag (Calculated using seacarb afte...)	21 CO2 [µmol/kg] (Calculated using seacarb afte...)	22 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	23 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	24 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	25 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	26 DIC [µmol/kg] (Calculated using seacarb afte...)	27 Omega Arg (Calculated using seacarb afte...)	28 Omega Cal (Calculated using seacarb afte...)
2	Echinometra mathaei (echinoderm)	A	7.67	A_acidified	1									2656	1217	34.6	25.1		8	35.70	1262.05	1258.09	2371.29	119.30	2526.29	1.90	2.88
2	Echinometra mathaei (echinoderm)	A	7.67	A_acidified	2	9.589								2656	1217	34.6	25.1		8	35.70	1262.05	1258.09	2371.29	119.30	2526.29	1.90	2.88
2	Echinometra mathaei (echinoderm)	A	7.67	A_acidified	3	20.101								2656	1217	34.6	25.1		8	35.70	1262.05	1258.09	2371.29	119.30	2526.29	1.90	2.88
2	Echinometra mathaei (echinoderm)	A	7.67	A_acidified	4	7.042								2656	1217	34.6	25.1		8	35.70	1262.05	1258.09	2371.29	119.30	2526.29	1.90	2.88
2	Echinometra mathaei (echinoderm)	A	7.67	A_acidified	5	19.138								2656	1217	34.6	25.1		8	35.70	1262.05	1258.09	2371.29	119.30	2526.29	1.90	2.88
2	Echinometra mathaei (echinoderm)	A	7.67	A_acidified	6	4.237								2656	1217	34.6	25.1		8	35.70	1262.05	1258.09	2371.29	119.30	2526.29	1.90	2.88
2	Echinometra mathaei (echinoderm)	A	7.67	A_acidified	7	4.713								2656	1217	34.6	25.1		8	35.70	1262.05	1258.09	2371.29	119.30	2526.29	1.90	2.88
2	Echinometra mathaei (echinoderm)	A	7.67	A_acidified	8	7.922								2656	1217	34.6	25.1		8	35.70	1262.05	1258.09	2371.29	119.30	2526.29	1.90	2.88
2	Echinometra mathaei (echinoderm)	A	7.67	A_acidified	9	31.619								2656	1217	34.6	25.1		8	35.70	1262.05	1258.09	2371.29	119.30	2526.29	1.90	2.88
2	Echinometra mathaei (echinoderm)	A	7.67	A_acidified	10	10.186								2656	1217	34.6	25.1		8	35.70	1262.05	1258.09	2371.29	119.30	2526.29	1.90	2.88
2	Echinometra mathaei (echinoderm)	A	7.67	A_acidified	11	19.929								2656	1217	34.6	25.1		8	35.70	1262.05	1258.09	2371.29	119.30	2526.29	1.90	2.88
2	Echinometra mathaei (echinoderm)	A	7.67	A_acidified	12	-8.119								2656	1217	34.6	25.1		8	35.70	1262.05	1258.09	2371.29	119.30	2526.29	1.90	2.88
2	Echinometra mathaei (echinoderm)	A	8.06	A_control	13	8.264								2612	475	34.6	25.5		8	12.22	436.48	435.12	2008.47	251.69	2272.39	4.01	6.08
2	Echinometra mathaei (echinoderm)	A	8.06	A_control	14	11.196								2612	475	34.6	25.5		8	12.22	436.48	435.12	2008.47	251.69	2272.39	4.01	6.08
2	Echinometra mathaei (echinoderm)	A	8.06	A_control	15	12.463								2612	475	34.6	25.5		8	12.22	436.48	435.12	2008.47	251.69	2272.39	4.01	6.08
2	Echinometra mathaei (echinoderm)	A	8.06	A_control	16	4.479								2612	475	34.6	25.5		8	12.22	436.48	435.12	2008.47	251.69	2272.39	4.01	6.08
2	Echinometra mathaei (echinoderm)	A	8.06	A_control	17	3.657								2612	475	34.6	25.5		8	12.22	436.48	435.12	2008.47	251.69	2272.39	4.01	6.08
2	Echinometra mathaei (echinoderm)	A	8.06	A_control	18	2.139								2612	475	34.6	25.5		8	12.22	436.48	435.12	2008.47	251.69	2272.39	4.01	6.08
2	Echinometra mathaei (echinoderm)	A	8.06	A_control	19	3.449								2612	475	34.6	25.5		8	12.22	436.48	435.12	2008.47	251.69	2272.39	4.01	6.08
2	Echinometra mathaei (echinoderm)	A	8.06	A_control	20	2.685								2612	475	34.6	25.5		8	12.22	436.48	435.12	2008.47	251.69	2272.39	4.01	6.08
2	Echinometra mathaei (echinoderm)	A	8.06	A_control	21	1.060								2612	475	34.6	25.5		8	12.22	436.48	435.12	2008.47	251.69	2272.39	4.01	6.08
2	Echinometra mathaei (echinoderm)	A	8.06	A_control	22	0.796								2612	475	34.6	25.5		8	12.22	436.48	435.12	2008.47	251.69	2272.39	4.01	6.08
2	Echinometra mathaei (echinoderm)	A	8.06	A_control	23	8.767								2612	475	34.6	25.5		8	12.22	436.48	435.12	2008.47	251.69	2272.39	4.01	6.08
2	Echinometra mathaei (echinoderm)	A	8.06	A_control	24	2.840								2612	475	34.6	25.5		8	12.22	436.48	435.12	2008.47	251.69	2272.39	4.01	6.08
2	Echinometra mathaei (echinoderm)	B	7.68	B_acidified	25	2.273								2611	1156	34.7	24.8		8	34.41	1207.62	1203.81	2326.65	118.74	2479.79	1.89	2.86
2	Echinometra mathaei (echinoderm)	B	7.68	B_acidified	26									2611	1156	34.7	24.8		8	34.41	1207.62	1203.81	2326.65	118.74	2479.79	1.89	2.86
2	Echinometra mathaei (echinoderm)	B	7.68	B_acidified	27	1.825								2611	1156	34.7	24.8		8	34.41	1207.62	1203.81	2326.65	118.74	2479.79	1.89	2.86
2	Echinometra mathaei (echinoderm)	B	7.68	B_acidified	28	7.288								2611	1156	34.7	24.8		8	34.41	1207.62	1203.81	2326.65	118.74	2479.79	1.89	2.86
2	Echinometra mathaei (echinoderm)	B	7.68	B_acidified	29	3.630								2611	1156	34.7	24.8		8	34.41	1207.62	1203.81	2326.65	118.74	2479.79	1.89	2.86
2	Echinometra mathaei (echinoderm)	B	7.68	B_acidified	30	23.495								2611	1156	34.7	24.8		8	34.41	1207.62	1203.81	2326.65	118.74	2479.79	1.89	2.86
2	Echinometra mathaei (echinoderm)	B	7.68	B_acidified	31	3.010								2611	1156	34.7	24.8		8	34.41	1207.62	1203.81	2326.65	118.74	2479.79	1.89	2.86
2	Echinometra mathaei (echinoderm)	B	7.68	B_acidified	32	-0.874								2611	1156	34.7	24.8		8	34.41	1207.62	1203.81	2326.65	118.74	2479.79	1.89	2.86
2	Echinometra mathaei (echinoderm)	B	7.68	B_acidified	33	3.134								2611	1156	34.7	24.8		8	34.41	1207.62	1203.81	2326.65	118.74	2479.79	1.89	2.86
2	Echinometra mathaei (echinoderm)	B	7.68	B_acidified	34									2611	1156	34.7	24.8		8	34.41	1207.62	1203.81	2326.65	118.74	2479.79	1.89	2.86
2	Echinometra mathaei (echinoderm)	B	7.68	B_acidified	35	3.083								2611	1156	34.7	24.8		8	34.41	1207.62	1203.81	2326.65	118.74	2479.79	1.89	2.86
2	Echinometra mathaei (echinoderm)	B	7.68	B_acidified	36	1.199								2611	1156	34.7	24.8		8	34.41	1207.62	1203.81	2326.65	118.74	2479.79	1.89	2.86
2	Echinometra mathaei (echinoderm)	B	8.07	B_control	37									2632	470	34.7	25.1		8	12.09	427.70	426.35	2019.17	255.75	2287.02	4.07	6.17
2	Echinometra mathaei (echinoderm)	B	8.07	B_control	38	15.832								2632	470	34.7	25.1		8	12.09	427.70	426.35	2019.17	255.75	2287.02	4.07	6.17
2	Echinometra mathaei (echinoderm)	B	8.07	B_control	39	1.017								2632	470	34.7	25.1		8	12.09	427.70	426.35	2019.17	255.75	2287.02	4.07	6.17
2	Echinometra mathaei (echinoderm)	B	8.07	B_control	40	28.959								2632	470	34.7	25.1		8	12.09	427.70	426.35	2019.17	255.75	2287.02	4.07	6.17
2	Echinometra mathaei (echinoderm)	B	8.07	B_control	41	3.085								2632	470	34.7	25.1		8	12.09	427.70	426.35	2019.17	255.75	2287.02	4.07	6.17
2	Echinometra mathaei (echinoderm)	B	8.07	B_control	42	13.727								2632	470	34.7	25.1		8	12.09	427.70	426.35	2019.17	255.75	2287.02	4.07	6.17
2	Echinometra mathaei (echinoderm)	B	8.07	B_control	43									2632	470	34.7	25.1		8	12.09	427.70	426.35	2019.17	255.75	2287.02	4.07	6.17
2	Echinometra mathaei (echinoderm)	B	8.07	B_control	44	-20.476								2632	470	34.7	25.1		8	12.09	427.70	426.35	2019.17	255.75	2287.02	4.07	6.17
2	Echinometra mathaei (echinoderm)	B	8.07	B_control	45	7.266								2632	470	34.7	25.1		8	12.09	427.70	426.35	2019.17	255.75	2287.02	4.07	6.17
2	Echinometra mathaei (echinoderm)	B	8.07	B_control	46	2.617								2632	470	34.7	25.1		8	12.09	427.70	426.35	2019.17	255.75	2287.02	4.07	6.17
2	Echinometra mathaei (echinoderm)	B	8.07	B_control	47	5.971								2632	470	34.7	25.1		8	12.09	427.70	426.35	2019.17	255.75	2287.02	4.07	6.17
2	Echinometra mathaei (echinoderm)	B	8.07	B_control	48	6.640								2632	470	34.7	25.1		8	12.09	427.70	426.35	2019.17	255.75	2287.02	4.07	6.17
3		A	7.67	A_acidified			0.790	1	2011-07-25					2910	1586	35.1	24.9		8	39.15	1380.41	1376.06	2600.49	131.37	2771.01	2.08	3.16
3		A	7.67	A_acidified			0.714	1	2011-08-01					2468	1425	34.9	24.8		8	33.25	1168.42	1164.73	2201.16	110.28	2344.70	1.75	2.65
3		A	7.67	A_acidified			1.238	1	2011-08-08					2217	1025	34.5	25.2		8	29.66	1050.60	1047.30	1972.23	99.35	2101.25	1.58	2.40
3		A	7.67	A_acidified			0.881	2	2011-07-25					2910	1586	35.1	24.9		8	39.15	1380.41	1376.06	2600.49	131.37	2771.01	2.08	3.16
3		A	7.67	A_acidified			0.790	2	2011-08-01					2468	1425	34.9	24.8		8	33.25	1168.42	1164.73	2201.16	110.28	2344.70	1.75	2.65
3		A	7.67	A_acidified			1.347	2	2011-08-08					2217	1025	34.5	25.2		8	29.66	1050.60	1047.30	1972.23	99.35	2101.25	1.58	2.40
3		A	8.06	A_control			0.848	3	2011-07-25					2874	526	35.1	25.0		8	13.59	480.46	478.95	2220.51	276.37	2510.48	4.38	6.64
3		A	8.06	A_control			0.718	3	2011-08-01					2456	465	34.8	25.0		8	11.59	409.15	407.86	1889.31	233.54	2134.44	3.71	5.63
3		A	8.06	A_control			0.836	3	2011-08-08					2198	446	34.5	25.8		8	10.14	364.59	363.45	1674.21	211.63	1895.98	3.38	5.12
3		A	8.06	A_control			0.810	4	2011-07-25					2874	526	35.1	25.0		8	13.59	480.46	478.95	2220.51	276.37	2510.48	4.38	6.64
3		A	8.06	A_control			0.846	4	2011-08-01					2456	465	34.8	25.0		8	11.59	409.15	407.86	1889.31	233.54	2134.44	3.71	5.63
3		A	8.06	A_control			1.011	4	2011-08-08					2198	446	34.5	25.8		8	10.14	364.59	363.45	1674.21	211.63	1895.98	3.38	5.12
3		B	7.68	B_acidified			0.451	5	2011-07-25					2760	1509	35.2	24.6		8	36.38	1273.69	1269.66	2460.31	126.08	2622.77	1.99	3.03
3		B	7.68	B_acidified			0.488	5	2011-08-01					2614	1449	35.0	24.5		8	34.57	1206.25	1202.43	2330.11	118.43	2483.12	1.87	2.85
3		B	7.68	B_acidified			0.592	5	2011-08-08					2773	1315	34.8	24.7		8	36.62	1282.93	1278.87	2473.91	126.08	2636.61	2.00	3.04
3		B	7.68	B_acidified			0.444	6	2011-07-25					2760	1509	35.2	24.6		8	36.38	1273.69	1269.66	2460.31	126.08	2622.77	1.99	3.03
3		B	7.68	B_acidified			0.758	6	2011-08-01					2614	1449	35.0	24.5		8	34.57	1206.25	1202.43	2330.11	118.43	2483.12	1.87	2.85
3		B	7.68	B_acidified			0.897	6	2011-08-08					2773	1315	34.8	24.7		8	36.62	1282.93	1278.87	2473.91	126.08	2636.61	2.00	3.04
3		B	8.07	B_control			0.461	7	2011-07-25					2736	492	35.1	24.7		8	12.66	444.13	442.73	2104.10	265.05	2381.80	4.20	6.37
3		B	8.07	B_control			0.657	7	2011-08-01					2602	523	34.9	24.6		8	12.08	422.40	421.06	2001.18	250.01	2263.27	3.96	6.02
3		B	8.07	B_control			0.756	7	2011-08-08					2747	429	34.7	25.2		8	12.61	447.02	445.61	2109.16	268.13	2389.89	4.26	6.47
3		B	8.07	B_control			0.838	8	2011-07-25					2736	492	35.1	24.7		8	12.66	444.13	442.73	2104.10	265.05	2381.80	4.20	6.37
3		B	8.07	B_control			0.885	8	2011-08-01					2602	523	34.9	24.6		8	12.08	422.40	421.06	2001.18	250.01	2263.27	3.96	6.02
3		B	8.07	B_control			0.449	8	2011-08-08					2747	429	34.7	25.2		8	12.61	447.02	445.61	2109.16	268.13	2389.89	4.26	6.47
4, 5		A	7.67	A_acidified	1				2011-07-26	26	7.432	4964.085	3745.597	2807	1338	35.0	25.2	4340.165	8	37.51	1332.07	1327.89	2504.35	127.62	2669.48	2.03	3.07
4, 5		A	7.67	A_acidified	2				2011-07-26	26	7.449	6034.012	4059.751	2807	1338	35.0	25.2	4900.134	8	37.51	1332.07	1327.89	2504.35	127.62	2669.48	2.03	3.07
4, 5		A	7.67	A_acidified	3				2011-07-26	26	7.412	5232.922	4259.984	2807	1338	35.0	25.2	4698.528	8	37.51	1332.07	1327.89	2504.35	127.62	2669.48	2.03	3.07
4, 5		A	7.67	A_acidified	4				2011-07-26	26	7.421	5191.171	3766.955	2807	1338	35.0	25.2	4249.742	8	37.51	1332.07	1327.89	2504.35	127.62	2669.48	2.03	3.07
4, 5		A	7.67	A_acidified	5				2011-07-26	26	7.400	4564.269	3937.470	2807	1338	35.0	25.2	3861.278	8	37.51	1332.07	1327.89	2504.35	127.62	2669.48	2.03	3.07
4, 5		A	7.67	A_acidified	6				2011-07-26	26	7.410	5433.228	4339.741	2807	1338	35.0	25.2	4762.826	8	37.51	1332.07	1327.89	2504.35	127.62	2669.48	2.03	3.07
4, 5		A	8.05	A_control	7				2011-07-26	26	7.338	2880.772	3405.387	2787	472	35.0	25.7	3140.171	8	13.30	478.27	476.78	2150.75	267.78	2431.83	4.26	6.45
4, 5		A	8.05	A_control	8				2011-07-26	26	7.359		3675.029	2787	472	35.0	25.7	3562.684	8	13.30	478.27	476.78	2150.75	267.78	2431.83	4.26	6.45
4, 5		A	8.05	A_control	9				2011-07-26	26	7.314	3577.640	3320.692	2787	472	35.0	25.7	2890.949	8	13.30	478.27	476.78	2150.75	267.78	2431.83	4.26	6.45
4, 5		A	8.05	A_control	10				2011-07-26	26	7.361	3441.372	2945.430	2787	472	35.0	25.7	2874.276	8	13.30	478.27	476.78	2150.75	267.78	2431.83	4.26	6.45
4, 5		A	8.05	A_control	11				2011-07-26	26	7.373	4332.273	3256.180	2787	472	35.0	25.7	3268.129	8	13.30	478.27	476.78	2150.75	267.78	2431.83	4.26	6.45
4, 5		A	8.05	A_control	12				2011-07-26	26	7.370	3861.633	3170.731	2787	472	35.0	25.7	3160.078	8	13.30	478.27	476.78	2150.75	267.78	2431.83	4.26	6.45
4, 5		B	7.67	B_acidified	13				2011-07-26	26	7.402	4782.976	3889.009	2770	1303	35.3	24.7	4202.122	8	37.32	1310.75	1306.61	2473.48	124.61	2635.42	1.97	2.99
4, 5		B	7.67	B_acidified	14				2011-07-26	26	7.318	4167.339	3783.284	2770	1303	35.3	24.7	3339.464	8	37.32	1310.75	1306.61	2473.48	124.61	2635.42	1.97	2.99
4, 5		B	7.67	B_acidified	15				2011-07-26	26	7.407	5453.453	4731.284	2770	1303	35.3	24.7	5168.432	8	37.32	1310.75	1306.61	2473.48	124.61	2635.42	1.97	2.99
4, 5		B	7.67	B_acidified	16				2011-07-26	26	7.379	5026.103	4556.743	2770	1303	35.3	24.7	4652.906	8	37.32	1310.75	1306.61	2473.48	124.61	2635.42	1.97	2.99
4, 5		B	7.67	B_acidified	17				2011-07-26	26	7.381	5062.368	3915.797	2770	1303	35.3	24.7	4021.483	8	37.32	1310.75	1306.61	2473.48	124.61	2635.42	1.97	2.99
4, 5		B	7.67	B_acidified	18				2011-07-26	26	7.413	5082.011	3891.918	2770	1303	35.3	24.7	4318.745	8	37.32	1310.75	1306.61	2473.48	124.61	2635.42	1.97	2.99
4, 5		B	8.08	B_control	19				2011-07-26	26	7.308	2847.879	2738.472	2749	438	35.2	25.4	2358.653	8	12.11	432.60	431.25	2089.28	276.95	2378.34	4.39	6.66
4, 5		B	8.08	B_control	20				2011-07-26	26	7.403	4437.965	3600.161	2749	438	35.2	25.4	4251.737	8	12.11	432.60	431.25	2089.28	276.95	2378.34	4.39	6.66
4, 5		B	8.08	B_control	21				2011-07-26	26	7.401	4188.500	3338.794	2749	438	35.2	25.4	3592.094	8	12.11	432.60	431.25	2089.28	276.95	2378.34	4.39	6.66
4, 5		B	8.08	B_control	22				2011-07-26	26	7.359	3642.698	3018.349	2749	438	35.2	25.4	2936.374	8	12.11	432.60	431.25	2089.28	276.95	2378.34	4.39	6.66
4, 5		B	8.08	B_control	23				2011-07-26	26	7.350	3363.028	2906.028	2749	438	35.2	25.4	2767.371	8	12.11	432.60	431.25	2089.28	276.95	2378.34	4.39	6.66
4, 5		B	8.08	B_control	24				2011-07-26	26	7.334	3933.996	3593.800	2749	438	35.2	25.4	3287.326	8	12.11	432.60	431.25	2089.28	276.95	2378.34	4.39	6.66
4, 5		A	7.65	A_acidified	1				2011-08-02	33	7.227	3956.668	4852.329	2441	1233	34.7	25.3	3423.511	8	34.29	1218.68	1214.86	2184.86	105.99	2325.13	1.69	2.56
4, 5		A	7.65	A_acidified	2				2011-08-02	33	7.382	5378.082	4123.148	2441	1233	34.7	25.3	4222.973	8	34.29	1218.68	1214.86	2184.86	105.99	2325.13	1.69	2.56
4, 5		A	7.65	A_acidified	3				2011-08-02	33	7.366	5070.458	4661.491	2441	1233	34.7	25.3	4590.184	8	34.29	1218.68	1214.86	2184.86	105.99	2325.13	1.69	2.56
4, 5		A	7.65	A_acidified	4				2011-08-02	33	7.382	4916.108	3906.988	2441	1233	34.7	25.3	4002.951	8	34.29	1218.68	1214.86	2184.86	105.99	2325.13	1.69	2.56
4, 5		A	7.65	A_acidified	5				2011-08-02	33	7.408	5248.831	4024.665	2441	1233	34.7	25.3	4390.344	8	34.29	1218.68	1214.86	2184.86	105.99	2325.13	1.69	2.56
4, 5		A	7.65	A_acidified	6				2011-08-02	33	7.368	4887.927	3899.036	2441	1233	34.7	25.3	3862.109	8	34.29	1218.68	1214.86	2184.86	105.99	2325.13	1.69	2.56
4, 5		A	8.05	A_control	7				2011-08-02	33	7.268	4365.147	3811.812	2412	414	34.8	25.7	2966.578	8	11.48	412.39	411.10	1853.38	229.70	2094.56	3.66	5.54
4, 5		A	8.05	A_control	8				2011-08-02	33	7.345	3927.268	3504.651	2412	414	34.8	25.7	3283.117	8	11.48	412.39	411.10	1853.38	229.70	2094.56	3.66	5.54
4, 5		A	8.05	A_control	9				2011-08-02	33	7.354	3209.012	2862.849	2412	414	34.8	25.7	2745.250	8	11.48	412.39	411.10	1853.38	229.70	2094.56	3.66	5.54
4, 5		A	8.05	A_control	10				2011-08-02	33	7.310	3118.103	2874.791	2412	414	34.8	25.7	2479.795	8	11.48	412.39	411.10	1853.38	229.70	2094.56	3.66	5.54
4, 5		A	8.05	A_control	11				2011-08-02	33	7.321	3818.180	3604.693	2412	414	34.8	25.7	3186.736	8	11.48	412.39	411.10	1853.38	229.70	2094.56	3.66	5.54
4, 5		A	8.05	A_control	12				2011-08-02	33	7.303	3322.800	3302.103	2412	414	34.8	25.7	2797.632	8	11.48	412.39	411.10	1853.38	229.70	2094.56	3.66	5.54
4, 5		B	7.68	B_acidified	13				2011-08-02	33	7.402	4811.791	4146.094	2612	1217	35.0	24.8	4470.855	8	34.30	1205.83	1202.02	2325.22	119.49	2479.00	1.89	2.87
4, 5		B	7.68	B_acidified	14				2011-08-02	33	7.315	3801.770		2612	1217	35.0	24.8		8	34.30	1205.83	1202.02	2325.22	119.49	2479.00	1.89	2.87
4, 5		B	7.68	B_acidified	15				2011-08-02	33	7.363	5126.752	4663.531	2612	1217	35.0	24.8	4573.747	8	34.30	1205.83	1202.02	2325.22	119.49	2479.00	1.89	2.87
4, 5		B	7.68	B_acidified	16				2011-08-02	33	7.382	4322.295	4045.073	2612	1217	35.0	24.8	4156.721	8	34.30	1205.83	1202.02	2325.22	119.49	2479.00	1.89	2.87
4, 5		B	7.68	B_acidified	17				2011-08-02	33	7.368	4640.324	3855.139	2612	1217	35.0	24.8	3831.153	8	34.30	1205.83	1202.02	2325.22	119.49	2479.00	1.89	2.87
4, 5		B	7.68	B_acidified	18				2011-08-02	33	7.349	4138.576	4005.271	2612	1217	35.0	24.8	3801.345	8	34.30	1205.83	1202.02	2325.22	119.49	2479.00	1.89	2.87
4, 5		B	8.06	B_control	19				2011-08-02	33	7.303	2762.318	2876.702	2607	434	34.9	25.4	2444.008	8	12.18	434.51	433.15	2002.35	251.75	2266.28	4.00	6.07
4, 5		B	8.06	B_control	20				2011-08-02	33	7.262	3480.130	3635.336	2607	434	34.9	25.4	2881.682	8	12.18	434.51	433.15	2002.35	251.75	2266.28	4.00	6.07
4, 5		B	8.06	B_control	21				2011-08-02	33	7.389	3325.284	2915.710	2607	434	34.9	25.4	3046.617	8	12.18	434.51	433.15	2002.35	251.75	2266.28	4.00	6.07
4, 5		B	8.06	B_control	22				2011-08-02	33	7.341	3502.649	2817.249	2607	434	34.9	25.4	2622.745	8	12.18	434.51	433.15	2002.35	251.75	2266.28	4.00	6.07
4, 5		B	8.06	B_control	23				2011-08-02	33	7.392	4097.423	3416.630	2607	434	34.9	25.4	3591.376	8	12.18	434.51	433.15	2002.35	251.75	2266.28	4.00	6.07
4, 5		B	8.06	B_control	24				2011-08-02	33	7.390	4126.944	3586.315	2607	434	34.9	25.4	3750.213	8	12.18	434.51	433.15	2002.35	251.75	2266.28	4.00	6.07
4, 5		A	7.70	A_acidified	1				2011-08-09	40	7.352	4176.335	3749.627	2293	998	34.2	25.4	2512.862	8	28.39	1009.05	1005.89	2025.41	109.37	2163.17	1.75	2.65
4, 5		A	7.70	A_acidified	2				2011-08-09	40	7.370	4501.693	3775.394	2293	998	34.2	25.4	3748.641	8	28.39	1009.05	1005.89	2025.41	109.37	2163.17	1.75	2.65
4, 5		A	7.70	A_acidified	3				2011-08-09	40	7.437	4859.411	3944.177	2293	998	34.2	25.4	4604.352	8	28.39	1009.05	1005.89	2025.41	109.37	2163.17	1.75	2.65
4, 5		A	7.70	A_acidified	4				2011-08-09	40	7.372	4510.088	4047.275	2293	998	34.2	25.4	4036.217	8	28.39	1009.05	1005.89	2025.41	109.37	2163.17	1.75	2.65
4, 5		A	7.70	A_acidified	5				2011-08-09	40	7.410	4396.758	3900.625	2293	998	34.2	25.4	4265.170	8	28.39	1009.05	1005.89	2025.41	109.37	2163.17	1.75	2.65
4, 5		A	7.70	A_acidified	6				2011-08-09	40	7.439	4470.598	3562.887	2293	998	34.2	25.4	4182.313	8	28.39	1009.05	1005.89	2025.41	109.37	2163.17	1.75	2.65
4, 5		A	8.07	A_control	7				2011-08-09	40	7.439	3687.634	3515.579	2217	358	34.2	26.2	4115.702	8	9.88	358.17	357.06	1677.55	218.66	1906.09	3.51	5.31
4, 5		A	8.07	A_control	8				2011-08-09	40	7.333	3160.332	3056.120	2217	358	34.2	26.2	2771.681	8	9.88	358.17	357.06	1677.55	218.66	1906.09	3.51	5.31
4, 5		A	8.07	A_control	9				2011-08-09	40	7.367	3214.919	2952.881	2217	358	34.2	26.2	2907.563	8	9.88	358.17	357.06	1677.55	218.66	1906.09	3.51	5.31
4, 5		A	8.07	A_control	10				2011-08-09	40	7.378	3228.051	2677.414	2217	358	34.2	26.2	2709.730	8	9.88	358.17	357.06	1677.55	218.66	1906.09	3.51	5.31
4, 5		A	8.07	A_control	11				2011-08-09	40	7.308	3056.847	3204.363	2217	358	34.2	26.2	2735.598	8	9.88	358.17	357.06	1677.55	218.66	1906.09	3.51	5.31
4, 5		A	8.07	A_control	12				2011-08-09	40	7.344	2912.822	2806.604	2217	358	34.2	26.2	2615.660	8	9.88	358.17	357.06	1677.55	218.66	1906.09	3.51	5.31
4, 5		B	7.70	B_acidified	13				2011-08-09	40	7.412	5632.487	4693.792	2793	1240	34.8	24.8	5177.364	8	34.98	1228.37	1224.49	2478.88	132.78	2646.63	2.11	3.20
4, 5		B	7.70	B_acidified	14				2011-08-09	40				2793	1240	34.8	24.8		8	34.98	1228.37	1224.49	2478.88	132.78	2646.63	2.11	3.20
4, 5		B	7.70	B_acidified	15				2011-08-09	40	7.335	5583.514	5597.194	2793	1240	34.8	24.8	5122.811	8	34.98	1228.37	1224.49	2478.88	132.78	2646.63	2.11	3.20
4, 5		B	7.70	B_acidified	16				2011-08-09	40	7.351	4838.045	4785.847	2793	1240	34.8	24.8	4555.584	8	34.98	1228.37	1224.49	2478.88	132.78	2646.63	2.11	3.20
4, 5		B	7.70	B_acidified	17				2011-08-09	40	7.419	5636.904	4595.649	2793	1240	34.8	24.8	5156.394	8	34.98	1228.37	1224.49	2478.88	132.78	2646.63	2.11	3.20
4, 5		B	7.70	B_acidified	18				2011-08-09	40	7.339	4317.801	4215.442	2793	1240	34.8	24.8	3901.260	8	34.98	1228.37	1224.49	2478.88	132.78	2646.63	2.11	3.20
4, 5		B	8.09	B_control	19				2011-08-09	40				2772	428	34.6	25.7		8	11.87	425.95	424.62	2097.77	283.76	2393.39	4.53	6.86
4, 5		B	8.09	B_control	20				2011-08-09	40	7.344	3399.564	3441.841	2772	428	34.6	25.7	3214.150	8	11.87	425.95	424.62	2097.77	283.76	2393.39	4.53	6.86
4, 5		B	8.09	B_control	21				2011-08-09	40	7.387	3560.490	3130.871	2772	428	34.6	25.7	3245.780	8	11.87	425.95	424.62	2097.77	283.76	2393.39	4.53	6.86
4, 5		B	8.09	B_control	22				2011-08-09	40	7.378	3586.518	3240.522	2772	428	34.6	25.7	3286.261	8	11.87	425.95	424.62	2097.77	283.76	2393.39	4.53	6.86
4, 5		B	8.09	B_control	23				2011-08-09	40	7.393	3001.870	2602.794	2772	428	34.6	25.7	2742.316	8	11.87	425.95	424.62	2097.77	283.76	2393.39	4.53	6.86
4, 5		B	8.09	B_control	24				2011-08-09	40	7.438	4030.269	3391.734	2772	428	34.6	25.7	3976.527	8	11.87	425.95	424.62	2097.77	283.76	2393.39	4.53	6.86