/* DATA DESCRIPTION: Citation: Rivest, Emily B; Hofmann, Gretchen E (2014): Responses of the metabolism of the larvae of Pocillopora damicornis to ocean acidification and warming. PANGAEA, https://doi.org/10.1594/PANGAEA.835576 Abstract: Ocean acidification and warming are expected to threaten the persistence of tropical coral reef ecosystems. As coral reefs face multiple stressors, the distribution and abundance of corals will depend on the successful dispersal and settlement of coral larvae under changing environmental conditions. To explore this scenario, we used metabolic rate, at holobiont and molecular levels, as an index for assessing the physiological plasticity of Pocillopora damicornis larvae from this site to conditions of ocean acidity and warming. Larvae were incubated for 6 hours in seawater containing combinations of CO2 concentration (450 and 950 µatm) and temperature (28 and 30°C). Rates of larval oxygen consumption were higher at elevated temperatures. In contrast, high CO2 levels elicited depressed metabolic rates, especially for larvae released later in the spawning period. Rates of citrate synthase, a rate-limiting enzyme in aerobic metabolism, suggested a biochemical limit for increasing oxidative capacity in coral larvae in a warming, acidifying ocean. Biological responses were also compared between larvae released from adult colonies on the same day (cohorts). The metabolic physiology of Pocillopora damicornis larvae varied significantly by day of release. Additionally, we used environmental data collected on a reef in Moorea, French Polynesia to provide information about what adult corals and larvae may currently experience in the field. An autonomous pH sensor provided a continuous time series of pH on the natal fringing reef. In February/March, 2011, pH values averaged 8.075±0.023. Our results suggest that without adaptation or acclimatization, only a portion of naïve Pocillopora damicornis larvae may have suitable metabolic phenotypes for maintaining function and fitness in an end-of-the century ocean. Keyword(s): Animalia; Cnidaria; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Laboratory experiment; Pelagos; Pocillopora damicornis; Respiration; Single species; South Pacific; Temperature; Tropical; Zooplankton Related to: Rivest, Emily B; Hofmann, Gretchen E (2014): Responses of the Metabolism of the Larvae of Pocillopora damicornis to Ocean Acidification and Warming. PLoS ONE, 9(4), e96172, https://doi.org/10.1371/journal.pone.0096172 Other version: Rivest, Emily B (2014): MCR LTER: Coral Reef: Coral Larval Metabolism in pH and Temperature Treatments. Moorea Coral Reef LTER Further details: Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb Project(s): Ocean Acidification International Coordination Centre (OA-ICC) (URI: https://www.iaea.org/services/oa-icc/science-and-collaboration/data-access-and-management) Coverage: LATITUDE: -17.480300 * LONGITUDE: -149.798900 DATE/TIME START: 2011-03-13T00:00:00 * DATE/TIME END: 2011-03-15T00:00:00 Event(s): Moorea_OA * LATITUDE: -17.480300 * LONGITUDE: -149.798900 * DATE/TIME START: 2011-03-04T00:00:00 * DATE/TIME END: 2011-03-14T00:00:00 * METHOD/DEVICE: Experiment (EXP) Comment: In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-09-03. Parameter(s): Species (Species) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) Duration, number of days [days] (Duration) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) Treatment (Treat) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) Replicate (Repl) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) Oxygen consumption per individual [nmol/#/min] (O2 con/ind) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) Oxygen consumption, per protein [µmol/g/min] (O2 con/protein) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) * COMMENT: per holobiont protein Proteins per individual [µg/#] (Prot/ind) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) * COMMENT: holobiont Citrate synthase activity per individual [µmol/min/#] (Citrate synthase activity/ind) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) Citrate synthase activity, per protein [nmol/min/mg] (CS act/protein) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) * COMMENT: per animal protein Proteins per individual [µg/#] (Prot/ind) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) * COMMENT: animal DATE/TIME (Date/Time) * GEOCODE Temperature, water [°C] (Temp) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) * COMMENT: low Temperature, water [°C] (Temp) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) * COMMENT: high Oxygen consumption per individual [nmol/#/min] (O2 con/ind) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) * COMMENT: at low temperature Oxygen consumption per individual [nmol/#/min] (O2 con/ind) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) * COMMENT: at high temperature Factor quantifying temperature dependent change of rates of processes (Q10) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) * COMMENT: oxygen consumption per larva Difference (Diff) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) * COMMENT: delta Q10, oxygen consumption per larva Oxygen consumption, per protein [µmol/g/min] (O2 con/protein) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) * COMMENT: at low temperature, per holobiont protein Oxygen consumption, per protein [µmol/g/min] (O2 con/protein) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) * COMMENT: at high temperature, per holobiont protein Factor quantifying temperature dependent change of rates of processes (Q10) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) * COMMENT: oxygen consumption per total holobiont protein Difference (Diff) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) * COMMENT: delta Q10, oxygen consumption per total holobiont protein Citrate synthase activity per individual [µmol/min/#] (Citrate synthase activity/ind) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) * COMMENT: at low temperature Citrate synthase activity per individual [µmol/min/#] (Citrate synthase activity/ind) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) * COMMENT: at high temperature Factor quantifying temperature dependent change of rates of processes (Q10) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) * COMMENT: citrate synthase activity per larva Difference (Diff) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) * COMMENT: delta Q10, citrate synthase activity per larva Citrate synthase activity, per protein [nmol/min/mg] (CS act/protein) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) * COMMENT: at low temperature, per animal protein Citrate synthase activity, per protein [nmol/min/mg] (CS act/protein) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) * COMMENT: at high temperature, per animal protein Factor quantifying temperature dependent change of rates of processes (Q10) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) * COMMENT: citrate synthase activity per total animal protein Difference (Diff) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) * COMMENT: delta Q10, citrate synthase activity per total animal protein Temperature, water [°C] (Temp) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) Temperature, water, standard error [±] (T std e) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) Salinity (Sal) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) Salinity, standard error [±] (Sal std e) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) pH (pH) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) * METHOD/DEVICE: Spectrophotometric * COMMENT: total scale Alkalinity, total [µmol/kg] (AT) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) * METHOD/DEVICE: Potentiometric titration Alkalinity, total, standard error [±] (AT std e) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) * METHOD/DEVICE: Potentiometric titration Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) [µatm] (pCO2water_SST_wet) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) * METHOD/DEVICE: Calculated using CO2calc Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error [±] (pCO2water_SST_wet std e) * PI: Rivest, Emily B (https://orcid.org/0000-0002-8570-8379, emily.rivest@lifesci.ucsb.edu) * METHOD/DEVICE: Calculated using CO2calc Carbonate system computation flag (CSC flag) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) Carbon dioxide [µmol/kg] (CO2) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) [µatm] (pCO2water_SST_wet) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) Fugacity of carbon dioxide (water) at sea surface temperature (wet air) [µatm] (fCO2water_SST_wet) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) Bicarbonate ion [µmol/kg] ([HCO3]-) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) Carbonate ion [µmol/kg] ([CO3]2-) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) Carbon, inorganic, dissolved [µmol/kg] (DIC) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) Aragonite saturation state (Omega Arg) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) Calcite saturation state (Omega Cal) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) License: Creative Commons Attribution 3.0 Unported (CC-BY-3.0) (URI: https://creativecommons.org/licenses/by/3.0/) Status: Curation Level: Enhanced curation (URI: https://wiki.pangaea.de/wiki/Curation_levels) Size: 2020 data points */ Species Duration [days] Treat Repl O2 con/ind [nmol/#/min] O2 con/protein [µmol/g/min] (per holobiont protein) Prot/ind [µg/#] (holobiont) Citrate synthase activity/ind [µmol/min/#] CS act/protein [nmol/min/mg] (per animal protein) Prot/ind [µg/#] (animal) Date/Time Temp [°C] (low) Temp [°C] (high) O2 con/ind [nmol/#/min] (at low temperature) O2 con/ind [nmol/#/min] (at high temperature) Q10 (oxygen consumption per larva) Diff (delta Q10, oxygen consumption...) O2 con/protein [µmol/g/min] (at low temperature, per holob...) O2 con/protein [µmol/g/min] (at high temperature, per holo...) Q10 (oxygen consumption per total ...) Diff (delta Q10, oxygen consumption...) Citrate synthase activity/ind [µmol/min/#] (at low temperature) Citrate synthase activity/ind [µmol/min/#] (at high temperature) Q10 (citrate synthase activity per...) Diff (delta Q10, citrate synthase a...) CS act/protein [nmol/min/mg] (at low temperature, per anima...) CS act/protein [nmol/min/mg] (at high temperature, per anim...) Q10 (citrate synthase activity per...) Diff (delta Q10, citrate synthase a...) Temp [°C] T std e [±] Sal Sal std e [±] pH (total scale, Spectrophotometric) AT [µmol/kg] (Potentiometric titration) AT std e [±] (Potentiometric titration) pCO2water_SST_wet [µatm] (Calculated using CO2calc) pCO2water_SST_wet std e [±] (Calculated using CO2calc) CSC flag (Calculated using seacarb afte...) CO2 [µmol/kg] (Calculated using seacarb afte...) pCO2water_SST_wet [µatm] (Calculated using seacarb afte...) fCO2water_SST_wet [µatm] (Calculated using seacarb afte...) [HCO3]- [µmol/kg] (Calculated using seacarb afte...) [CO3]2- [µmol/kg] (Calculated using seacarb afte...) DIC [µmol/kg] (Calculated using seacarb afte...) Omega Arg (Calculated using seacarb afte...) Omega Cal (Calculated using seacarb afte...) Pocillopora damicornis (coral) 9 Ambient CO2, low temperature 1 0.07752415 9.06079326 8.556 7946.109069 0.001656349 4.797364325 27.50 35.33 8.018 2353 13 435.93 6.00 8 11.58 435.81 434.47 1803.77 224.45 2039.81 3.60 5.41 Pocillopora damicornis (coral) 9 Ambient CO2, low temperature 2 0.07083331 11.00750720 6.435 10333.785820 0.001598644 6.464094847 27.50 35.33 8.018 2353 13 435.93 6.00 8 11.58 435.81 434.47 1803.77 224.45 2039.81 3.60 5.41 Pocillopora damicornis (coral) 9 Ambient CO2, low temperature 3 0.09299780 9.31935093 9.979 6427.334996 0.001504078 4.273273417 27.50 35.33 8.018 2353 13 435.93 6.00 8 11.58 435.81 434.47 1803.77 224.45 2039.81 3.60 5.41 Pocillopora damicornis (coral) 9 Ambient CO2, low temperature 4 0.09527837 7.45585531 12.779 9613.475825 0.001584139 6.068581701 27.50 35.33 8.018 2353 13 435.93 6.00 8 11.58 435.81 434.47 1803.77 224.45 2039.81 3.60 5.41 Pocillopora damicornis (coral) 9 Ambient CO2, low temperature 5 0.12389935 9.18998323 13.482 5878.357613 0.001278257 4.598728885 27.50 35.33 8.018 2353 13 435.93 6.00 8 11.58 435.81 434.47 1803.77 224.45 2039.81 3.60 5.41 Pocillopora damicornis (coral) 9 Ambient CO2, low temperature 6 0.09388251 6.36017251 14.761 8424.165591 0.001455823 5.786533077 27.50 35.33 8.018 2353 13 435.93 6.00 8 11.58 435.81 434.47 1803.77 224.45 2039.81 3.60 5.41 Pocillopora damicornis (coral) 9 Ambient CO2, high temperature 1 0.09075055 6.24573669 14.530 7569.222068 0.001520939 4.976676845 30.70 0.1 35.40 7.985 2364 7 476.62 3.60 8 11.74 476.17 474.77 1795.55 232.77 2040.06 3.79 5.64 Pocillopora damicornis (coral) 9 Ambient CO2, high temperature 2 0.09801252 10.58222000 9.262 8601.010101 0.001524012 5.643662392 30.70 0.1 35.40 7.985 2364 7 476.62 3.60 8 11.74 476.17 474.77 1795.55 232.77 2040.06 3.79 5.64 Pocillopora damicornis (coral) 9 Ambient CO2, high temperature 3 0.14052996 14.72442950 9.544 12158.788930 0.001667995 7.289464500 30.70 0.1 35.40 7.985 2364 7 476.62 3.60 8 11.74 476.17 474.77 1795.55 232.77 2040.06 3.79 5.64 Pocillopora damicornis (coral) 9 Ambient CO2, high temperature 4 0.14153346 10.41912990 13.584 9221.875000 0.001531718 6.020607670 30.70 0.1 35.40 7.985 2364 7 476.62 3.60 8 11.74 476.17 474.77 1795.55 232.77 2040.06 3.79 5.64 Pocillopora damicornis (coral) 9 Ambient CO2, high temperature 5 0.14120320 11.03408580 12.797 8616.386994 0.001267229 6.799393813 30.70 0.1 35.40 7.985 2364 7 476.62 3.60 8 11.74 476.17 474.77 1795.55 232.77 2040.06 3.79 5.64 Pocillopora damicornis (coral) 9 Ambient CO2, high temperature 6 0.13006371 12.81037280 10.153 8066.935107 0.001418937 5.685194159 30.70 0.1 35.40 7.985 2364 7 476.62 3.60 8 11.74 476.17 474.77 1795.55 232.77 2040.06 3.79 5.64 Pocillopora damicornis (coral) 9 High CO2, low temperature 1 0.08322557 8.11402612 10.257 9049.910873 0.001413396 6.402956354 28.10 35.43 7.714 2354 16 995.54 11.82 8 26.05 995.16 992.14 2039.19 129.07 2194.31 2.07 3.11 Pocillopora damicornis (coral) 9 High CO2, low temperature 2 0.09589879 9.52227036 10.071 7279.995331 0.001501290 4.849159394 28.10 35.43 7.714 2354 16 995.54 11.82 8 26.05 995.16 992.14 2039.19 129.07 2194.31 2.07 3.11 Pocillopora damicornis (coral) 9 High CO2, low temperature 3 0.07684669 9.79687497 7.844 10549.596310 0.001568249 6.726990366 28.10 35.43 7.714 2354 16 995.54 11.82 8 26.05 995.16 992.14 2039.19 129.07 2194.31 2.07 3.11 Pocillopora damicornis (coral) 9 High CO2, low temperature 4 0.07501439 10.38836610 7.221 8254.307784 0.001513811 5.452667762 28.10 35.43 7.714 2354 16 995.54 11.82 8 26.05 995.16 992.14 2039.19 129.07 2194.31 2.07 3.11 Pocillopora damicornis (coral) 9 High CO2, low temperature 5 0.06859969 6.14747668 11.159 8505.193744 0.001550824 5.484305101 28.10 35.43 7.714 2354 16 995.54 11.82 8 26.05 995.16 992.14 2039.19 129.07 2194.31 2.07 3.11 Pocillopora damicornis (coral) 9 High CO2, low temperature 6 0.12024663 9.02888062 13.318 6749.824930 0.001562545 4.319762380 28.10 35.43 7.714 2354 16 995.54 11.82 8 26.05 995.16 992.14 2039.19 129.07 2194.31 2.07 3.11 Pocillopora damicornis (coral) 9 High CO2, high temperature 1 0.11036325 8.34063255 13.232 8693.935636 0.001477392 5.884651468 30.44 0.1 35.63 0.1 7.736 2383 5 952.10 9.98 8 23.55 950.67 947.86 2024.63 147.33 2195.50 2.39 3.56 Pocillopora damicornis (coral) 9 High CO2, high temperature 2 0.10447439 8.93325304 11.695 10139.227290 0.001458864 6.950084550 30.44 0.1 35.63 0.1 7.736 2383 5 952.10 9.98 8 23.55 950.67 947.86 2024.63 147.33 2195.50 2.39 3.56 Pocillopora damicornis (coral) 9 High CO2, high temperature 3 0.11449978 8.48146522 13.500 8804.668465 0.001389836 6.335039089 30.44 0.1 35.63 0.1 7.736 2383 5 952.10 9.98 8 23.55 950.67 947.86 2024.63 147.33 2195.50 2.39 3.56 Pocillopora damicornis (coral) 9 High CO2, high temperature 4 0.10723783 12.73911010 8.418 8669.139401 0.001374397 6.307596116 30.44 0.1 35.63 0.1 7.736 2383 5 952.10 9.98 8 23.55 950.67 947.86 2024.63 147.33 2195.50 2.39 3.56 Pocillopora damicornis (coral) 9 High CO2, high temperature 5 0.13458553 10.10250200 13.322 10685.016770 0.001311694 8.145965536 30.44 0.1 35.63 0.1 7.736 2383 5 952.10 9.98 8 23.55 950.67 947.86 2024.63 147.33 2195.50 2.39 3.56 Pocillopora damicornis (coral) 9 High CO2, high temperature 6 10596.986740 0.001487613 7.123482864 30.44 0.1 35.63 0.1 7.736 2383 5 952.10 9.98 8 23.55 950.67 947.86 2024.63 147.33 2195.50 2.39 3.56 Pocillopora damicornis (coral) 10 Ambient CO2, low temperature 1 0.10626348 8.31873145 12.774 11273.126810 0.001375466 8.195857348 27.50 35.33 8.018 2353 13 435.93 6.00 8 11.58 435.81 434.47 1803.77 224.45 2039.81 3.60 5.41 Pocillopora damicornis (coral) 10 Ambient CO2, low temperature 2 0.14936939 8.89421155 16.794 10068.138690 0.001391937 7.233185317 27.50 35.33 8.018 2353 13 435.93 6.00 8 11.58 435.81 434.47 1803.77 224.45 2039.81 3.60 5.41 Pocillopora damicornis (coral) 10 Ambient CO2, low temperature 3 0.15858846 10.95829590 14.472 10155.822940 0.001387128 7.321476431 27.50 35.33 8.018 2353 13 435.93 6.00 8 11.58 435.81 434.47 1803.77 224.45 2039.81 3.60 5.41 Pocillopora damicornis (coral) 10 Ambient CO2, low temperature 4 0.11095452 8.98417162 12.350 9423.947094 0.001336365 7.051923694 27.50 35.33 8.018 2353 13 435.93 6.00 8 11.58 435.81 434.47 1803.77 224.45 2039.81 3.60 5.41 Pocillopora damicornis (coral) 10 Ambient CO2, low temperature 5 0.11804202 7.95753101 14.834 12803.399470 0.001553538 8.241446236 27.50 35.33 8.018 2353 13 435.93 6.00 8 11.58 435.81 434.47 1803.77 224.45 2039.81 3.60 5.41 Pocillopora damicornis (coral) 10 Ambient CO2, low temperature 10619.138680 0.001449090 7.328142412 27.50 35.33 8.018 2353 13 435.93 6.00 8 11.58 435.81 434.47 1803.77 224.45 2039.81 3.60 5.41 Pocillopora damicornis (coral) 10 Ambient CO2, high temperature 1 0.11750116 10.65963510 11.023 11754.751130 0.001493379 7.871245470 30.70 0.1 35.40 7.985 2364 7 476.62 3.60 8 11.74 476.17 474.77 1795.55 232.77 2040.06 3.79 5.64 Pocillopora damicornis (coral) 10 Ambient CO2, high temperature 2 0.15595055 11.41909270 13.657 12496.871010 0.001694750 7.373872680 30.70 0.1 35.40 7.985 2364 7 476.62 3.60 8 11.74 476.17 474.77 1795.55 232.77 2040.06 3.79 5.64 Pocillopora damicornis (coral) 10 Ambient CO2, high temperature 3 0.12121995 9.35339118 12.960 9458.446456 0.001774629 5.329815250 30.70 0.1 35.40 7.985 2364 7 476.62 3.60 8 11.74 476.17 474.77 1795.55 232.77 2040.06 3.79 5.64 Pocillopora damicornis (coral) 10 Ambient CO2, high temperature 4 0.14086970 8.83971534 15.936 10774.159660 0.001653589 6.515620825 30.70 0.1 35.40 7.985 2364 7 476.62 3.60 8 11.74 476.17 474.77 1795.55 232.77 2040.06 3.79 5.64 Pocillopora damicornis (coral) 10 Ambient CO2, high temperature 5 0.16126853 9.87620397 16.329 10853.009980 0.001634360 6.640525607 30.70 0.1 35.40 7.985 2364 7 476.62 3.60 8 11.74 476.17 474.77 1795.55 232.77 2040.06 3.79 5.64 Pocillopora damicornis (coral) 10 Ambient CO2, high temperature 10410.976890 0.001514803 6.872826455 30.70 0.1 35.40 7.985 2364 7 476.62 3.60 8 11.74 476.17 474.77 1795.55 232.77 2040.06 3.79 5.64 Pocillopora damicornis (coral) 10 High CO2, low temperature 1 0.11198684 9.64323112 11.613 13577.422150 0.001358958 9.991055929 28.10 35.43 7.714 2354 16 995.54 11.82 8 26.05 995.16 992.14 2039.19 129.07 2194.31 2.07 3.11 Pocillopora damicornis (coral) 10 High CO2, low temperature 2 0.10154497 8.31245643 12.216 13756.476530 0.001385106 9.931714958 28.10 35.43 7.714 2354 16 995.54 11.82 8 26.05 995.16 992.14 2039.19 129.07 2194.31 2.07 3.11 Pocillopora damicornis (coral) 10 High CO2, low temperature 3 0.13895893 14.02350650 9.909 8016.748366 0.001472739 5.443426670 28.10 35.43 7.714 2354 16 995.54 11.82 8 26.05 995.16 992.14 2039.19 129.07 2194.31 2.07 3.11 Pocillopora damicornis (coral) 10 High CO2, low temperature 4 0.11529898 9.92758581 11.614 10845.328280 0.001507364 7.194897105 28.10 35.43 7.714 2354 16 995.54 11.82 8 26.05 995.16 992.14 2039.19 129.07 2194.31 2.07 3.11 Pocillopora damicornis (coral) 10 High CO2, low temperature 5 0.11211227 8.75740284 12.802 10639.443280 0.001278912 8.319138107 28.10 35.43 7.714 2354 16 995.54 11.82 8 26.05 995.16 992.14 2039.19 129.07 2194.31 2.07 3.11 Pocillopora damicornis (coral) 10 High CO2, low temperature 6 0.10035825 7.77790048 12.903 9672.577855 0.001403748 6.890536105 28.10 35.43 7.714 2354 16 995.54 11.82 8 26.05 995.16 992.14 2039.19 129.07 2194.31 2.07 3.11 Pocillopora damicornis (coral) 10 High CO2, high temperature 1 0.17233546 15.20383440 11.335 8983.062240 0.001375606 6.530260400 30.44 0.1 35.63 0.1 7.736 2383 5 952.10 9.98 8 23.55 950.67 947.86 2024.63 147.33 2195.50 2.39 3.56 Pocillopora damicornis (coral) 10 High CO2, high temperature 2 0.11289479 8.96630878 12.591 10969.834090 0.001567434 6.998595067 30.44 0.1 35.63 0.1 7.736 2383 5 952.10 9.98 8 23.55 950.67 947.86 2024.63 147.33 2195.50 2.39 3.56 Pocillopora damicornis (coral) 10 High CO2, high temperature 3 0.12638956 11.21071150 11.274 11537.351190 0.001777763 6.489815352 30.44 0.1 35.63 0.1 7.736 2383 5 952.10 9.98 8 23.55 950.67 947.86 2024.63 147.33 2195.50 2.39 3.56 Pocillopora damicornis (coral) 10 High CO2, high temperature 4 0.13907546 9.01974591 15.419 10933.051920 0.001542655 7.087165949 30.44 0.1 35.63 0.1 7.736 2383 5 952.10 9.98 8 23.55 950.67 947.86 2024.63 147.33 2195.50 2.39 3.56 Pocillopora damicornis (coral) 10 High CO2, high temperature 5 0.15267863 8.20808713 18.601 12839.940320 0.001669274 7.691931190 30.44 0.1 35.63 0.1 7.736 2383 5 952.10 9.98 8 23.55 950.67 947.86 2024.63 147.33 2195.50 2.39 3.56 Pocillopora damicornis (coral) 10 High CO2, high temperature 6 0.13155422 7.05573742 18.645 8467.046715 0.001357449 6.237468900 30.44 0.1 35.63 0.1 7.736 2383 5 952.10 9.98 8 23.55 950.67 947.86 2024.63 147.33 2195.50 2.39 3.56 Pocillopora damicornis (coral) 11 Ambient CO2, low temperature 1 0.09145904 5.86501495 15.594 13346.429130 0.001438927 9.275265657 27.50 35.33 8.018 2353 13 435.93 6.00 8 11.58 435.81 434.47 1803.77 224.45 2039.81 3.60 5.41 Pocillopora damicornis (coral) 11 Ambient CO2, low temperature 2 0.07320015 5.63034799 13.001 9916.270856 0.001493310 6.640464253 27.50 35.33 8.018 2353 13 435.93 6.00 8 11.58 435.81 434.47 1803.77 224.45 2039.81 3.60 5.41 Pocillopora damicornis (coral) 11 Ambient CO2, low temperature 3 0.09546587 6.58249114 14.503 11346.405230 0.001434718 7.908458354 27.50 35.33 8.018 2353 13 435.93 6.00 8 11.58 435.81 434.47 1803.77 224.45 2039.81 3.60 5.41 Pocillopora damicornis (coral) 11 Ambient CO2, low temperature 4 0.09217825 6.82195456 13.512 10137.196890 0.001500099 6.757684260 27.50 35.33 8.018 2353 13 435.93 6.00 8 11.58 435.81 434.47 1803.77 224.45 2039.81 3.60 5.41 Pocillopora damicornis (coral) 11 Ambient CO2, low temperature 5 0.09582060 6.87329465 13.941 10034.806820 0.001564828 6.412722524 27.50 35.33 8.018 2353 13 435.93 6.00 8 11.58 435.81 434.47 1803.77 224.45 2039.81 3.60 5.41 Pocillopora damicornis (coral) 11 Ambient CO2, low temperature 6 0.08943953 7.75576950 11.532 9270.260695 0.001571054 5.900663832 27.50 35.33 8.018 2353 13 435.93 6.00 8 11.58 435.81 434.47 1803.77 224.45 2039.81 3.60 5.41 Pocillopora damicornis (coral) 11 Ambient CO2, high temperature 1 0.12071194 9.99601999 12.076 10392.769610 0.001107735 9.382000893 30.70 0.1 35.40 7.985 2364 7 476.62 3.60 8 11.74 476.17 474.77 1795.55 232.77 2040.06 3.79 5.64 Pocillopora damicornis (coral) 11 Ambient CO2, high temperature 2 0.11974069 10.21852650 11.718 8123.747108 0.001046061 7.766039066 30.70 0.1 35.40 7.985 2364 7 476.62 3.60 8 11.74 476.17 474.77 1795.55 232.77 2040.06 3.79 5.64 Pocillopora damicornis (coral) 11 Ambient CO2, high temperature 3 0.13108718 12.16247680 10.778 6406.287824 0.001058948 6.049673377 30.70 0.1 35.40 7.985 2364 7 476.62 3.60 8 11.74 476.17 474.77 1795.55 232.77 2040.06 3.79 5.64 Pocillopora damicornis (coral) 11 Ambient CO2, high temperature 4 0.14707591 8.73839380 16.831 7394.228525 0.001143486 6.466392898 30.70 0.1 35.40 7.985 2364 7 476.62 3.60 8 11.74 476.17 474.77 1795.55 232.77 2040.06 3.79 5.64 Pocillopora damicornis (coral) 11 Ambient CO2, high temperature 5 0.13102958 7.65583291 17.115 9989.058123 0.001337922 7.466101232 30.70 0.1 35.40 7.985 2364 7 476.62 3.60 8 11.74 476.17 474.77 1795.55 232.77 2040.06 3.79 5.64 Pocillopora damicornis (coral) 11 Ambient CO2, high temperature 6 7902.111614 0.001061304 7.445661941 30.70 0.1 35.40 7.985 2364 7 476.62 3.60 8 11.74 476.17 474.77 1795.55 232.77 2040.06 3.79 5.64 Pocillopora damicornis (coral) 11 High CO2, low temperature 1 0.07758783 6.58081722 11.790 8786.041014 0.001435312 6.121347077 28.10 35.43 7.714 2354 16 995.54 11.82 8 26.05 995.16 992.14 2039.19 129.07 2194.31 2.07 3.11 Pocillopora damicornis (coral) 11 High CO2, low temperature 2 0.07065848 6.16619943 11.459 7569.141854 0.001373254 5.511830900 28.10 35.43 7.714 2354 16 995.54 11.82 8 26.05 995.16 992.14 2039.19 129.07 2194.31 2.07 3.11 Pocillopora damicornis (coral) 11 High CO2, low temperature 3 0.06475454 5.74880544 11.264 10166.365010 0.001306179 7.783284132 28.10 35.43 7.714 2354 16 995.54 11.82 8 26.05 995.16 992.14 2039.19 129.07 2194.31 2.07 3.11 Pocillopora damicornis (coral) 11 High CO2, low temperature 4 0.10315916 7.70304367 13.392 10147.488820 0.001283975 7.903181463 28.10 35.43 7.714 2354 16 995.54 11.82 8 26.05 995.16 992.14 2039.19 129.07 2194.31 2.07 3.11 Pocillopora damicornis (coral) 11 High CO2, low temperature 5 0.09804036 6.69766119 14.638 8698.385236 0.001323633 6.571597662 28.10 35.43 7.714 2354 16 995.54 11.82 8 26.05 995.16 992.14 2039.19 129.07 2194.31 2.07 3.11 Pocillopora damicornis (coral) 11 High CO2, low temperature 6 0.08169673 7.62096368 10.720 8087.226067 0.001419547 5.697047097 28.10 35.43 7.714 2354 16 995.54 11.82 8 26.05 995.16 992.14 2039.19 129.07 2194.31 2.07 3.11 Pocillopora damicornis (coral) 11 High CO2, high temperature 1 0.10674453 7.44538823 14.337 9504.977376 0.001218070 7.803312008 30.44 0.1 35.63 0.1 7.736 2383 5 952.10 9.98 8 23.55 950.67 947.86 2024.63 147.33 2195.50 2.39 3.56 Pocillopora damicornis (coral) 11 High CO2, high temperature 2 0.06874210 7.23753395 9.498 8145.127748 0.001270422 6.411354373 30.44 0.1 35.63 0.1 7.736 2383 5 952.10 9.98 8 23.55 950.67 947.86 2024.63 147.33 2195.50 2.39 3.56 Pocillopora damicornis (coral) 11 High CO2, high temperature 3 0.13892130 11.52300060 12.056 8045.222041 0.001294110 6.216797655 30.44 0.1 35.63 0.1 7.736 2383 5 952.10 9.98 8 23.55 950.67 947.86 2024.63 147.33 2195.50 2.39 3.56 Pocillopora damicornis (coral) 11 High CO2, high temperature 4 0.11256562 9.32838456 12.067 9154.703548 0.001226705 7.462838123 30.44 0.1 35.63 0.1 7.736 2383 5 952.10 9.98 8 23.55 950.67 947.86 2024.63 147.33 2195.50 2.39 3.56 Pocillopora damicornis (coral) 11 High CO2, high temperature 5 0.10453038 11.77276500 8.879 9161.414566 0.001272897 7.197296604 30.44 0.1 35.63 0.1 7.736 2383 5 952.10 9.98 8 23.55 950.67 947.86 2024.63 147.33 2195.50 2.39 3.56 Pocillopora damicornis (coral) 11 High CO2, high temperature 6 0.12160812 10.45371950 11.633 8993.166964 0.001249520 7.197296604 30.44 0.1 35.63 0.1 7.736 2383 5 952.10 9.98 8 23.55 950.67 947.86 2024.63 147.33 2195.50 2.39 3.56 Pocillopora damicornis (coral) Ambient CO2 2011-03-13 27.500 30.8 0.092402582 0.123682234 2.387699870 0.983699554 8.732277074 10.969329120 1.976 -0.453 8103.871486 9039.036366 1.385418112 0.413317676 0.001512881 0.001488472 0.953 -0.215 Pocillopora damicornis (coral) High CO2 2011-03-13 28.200 30.5 0.086638626 0.114232157 3.371399424 8.832982475 9.719392567 1.522 8398.138162 9598.162383 1.798735788 0.001518353 0.001416633 0.737 Pocillopora damicornis (coral) Ambient CO2 2011-03-14 27.400 30.8 0.128643571 0.139361979 1.265389322 1.327790260 9.022588306 10.029607660 1.365 -0.264 10723.928940 10958.035850 1.065576599 -0.245558794 0.001415587 0.001627585 1.507 0.084 Pocillopora damicornis (coral) High CO2 2011-03-14 28.200 30.3 0.113376707 0.139154689 2.593179582 9.740347199 9.944070858 1.101 11084.666080 10621.714410 0.820017806 0.001401138 0.001548363 1.592 Pocillopora damicornis (coral) Ambient CO2 2011-03-15 27.500 30.5 0.089593908 0.134694248 3.892559154 -1.065687639 6.588145464 9.754250004 3.699 0.112 10675.228270 8368.033800 0.444106897 0.524492300 0.001500489 0.001125909 0.384 0.361 Pocillopora damicornis (coral) High CO2 2011-03-15 28.000 30.6 0.082649519 0.108852007 2.826871514 6.752915104 9.626798641 3.812 8909.108000 8834.102040 0.968599197 0.001356983 0.001255287 0.745