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King, Andrew L; Sañudo-Wilhelmy, Sergio A; Leblanc, Karine; Hutchins, David A; Fu, Feixue (2011): CO2 and vitamin B12 interactions determine bioactive trace metal requirements of a subarctic Pacific diatom [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.835475

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Abstract:
Phytoplankton growth can be limited by numerous inorganic nutrients and organic growth factors. Using the subarctic diatom Attheya sp. in culture studies, we examined how the availability of vitamin B(12) and carbon dioxide partial pressure (pCO(2)) influences growth rate, primary productivity, cellular iron (Fe), cobalt (Co), zinc (Zn) and cadmium (Cd) quotas, and the net use efficiencies (NUEs) of these bioactive trace metals (mol C fixed per mol cellular trace metal per day). Under B(12)-replete conditions, cells grown at high pCO(2) had lower Fe, Zn and Cd quotas, and used those trace metals more efficiently in comparison with cells grown at low pCO(2). At high pCO(2), B(12)-limited cells had ~50% lower specific growth and carbon fixation rates, and used Fe ~15-fold less efficiently, and Zn and Cd ~3-fold less efficiently, in comparison with B(12)-replete cells. The observed higher Fe, Zn and Cd NUE under high pCO(2)/B(12)-replete conditions are consistent with predicted downregulation of carbon-concentrating mechanisms. Co quotas of B(12)-replete cells were 5- to 14-fold higher in comparison with B(12)-limited cells, suggesting that >80% of cellular Co of B(12)-limited cells was likely from B(12). Our results demonstrate that CO(2) and vitamin B(12) interactively influence growth, carbon fixation, trace metal requirements and trace metal NUE of this diatom. This suggests the need to consider complex feedback interactions between multiple environmental factors for this biogeochemically critical group of phytoplankton in the last glacial maximum as well as the current and future changing ocean.
Keyword(s):
Attheya sp.; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Micro-nutrients; North Pacific; Ochrophyta; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species
Related to:
King, Andrew L; Sañudo-Wilhelmy, Sergio A; Leblanc, Karine; Hutchins, David A; Fu, Feixue (2011): CO2 and vitamin B12 interactions determine bioactive trace metal requirements of a subarctic Pacific diatom. The ISME Journal, 5(8), 1388-1396, https://doi.org/10.1038/ismej.2010.211
Original version:
Fu, Feixue; Hutchins, David A; Sañudo-Wilhelmy, Sergio A (2012): Dataset: Attheya sp. experiments. Biological and Chemical Oceanography Data Management Office, https://www.bco-dmo.org/dataset/3770
Further details:
Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb
Project(s):
Ocean Acidification International Coordination Centre (OA-ICC)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-09-01.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1 SpeciesSpeciesKing, Andrew L
2 TreatmentTreatKing, Andrew L
3 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetppmvKing, Andrew Ltreatment
4 Growth rateµ1/dayKing, Andrew L
5 Growth rate, standard deviationµ std dev±King, Andrew L
6 Primary productionPPµg/µg/hKing, Andrew L
7 Primary production of carbon, standard deviationPP C std dev±King, Andrew L
8 Carbon/Phosphorus ratioC/PKing, Andrew L
9 Carbon/Phosphorus ratio, standard deviationC/P std dev±King, Andrew L
10 Nitrogen/Phosphorus ratioN/PKing, Andrew L
11 Nitrogen/Phosphorus ratio, standard deviationN/P std dev±King, Andrew L
12 Iron/Phosphorus ratioFe/PKing, Andrew L
13 Iron/Phosphorus ratio, standard deviationFe/P std dev±King, Andrew L
14 Cobalt/Phosphorus ratioCo/PKing, Andrew L
15 Cobalt/Phosphorus ratio, standard deviationCo/P std dev±King, Andrew L
16 Zinc/Phosphorus ratioZn/PKing, Andrew L
17 Zinc/Phosphorus ratio, standard deviationZn/P std dev±King, Andrew L
18 Cadmium/Phosphorus ratioCd/PKing, Andrew L
19 Cadmium/Phosphorus ratio, standard deviationCd/P std dev±King, Andrew L
20 Net use efficiency, IronNUE Femol/mol/dayKing, Andrew Lmol C fixed per mol Fe per day
21 Net use efficiency, Iron, standard deviationNUE Fe std dev±King, Andrew L
22 Net use efficiency, CobaltNUE Comol/mol/dayKing, Andrew Lmol C fixed per mol Co per day
23 Net use efficiency, Cobalt, standard deviationNUE Co std dev±King, Andrew L
24 Net use efficiency, ZincNUE Znmol/mol/dayKing, Andrew Lmol C fixed per mol Zn per day
25 Net use efficiency, Zinc, standard deviationNUE Zn std dev±King, Andrew L
26 Net use efficiency, CadmiumNUE Cdmol/mol/dayKing, Andrew Lmol C fixed per mol Cd per day
27 Net use efficiency, Cadmium, standard deviationNUE Cd std dev±King, Andrew L
28 SalinitySalKing, Andrew L
29 Temperature, waterTemp°CKing, Andrew L
30 pHpHKing, Andrew LPotentiometricNBS scale
31 pH, standard deviationpH std dev±King, Andrew LPotentiometricNBS scale
32 Carbon, inorganic, dissolvedDICµmol/kgKing, Andrew LCoulometric titration
33 Carbon, inorganic, dissolved, standard deviationDIC std dev±King, Andrew LCoulometric titration
34 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetppmvKing, Andrew LCalculated using CO2SYS
35 Partial pressure of carbon dioxide, standard deviationpCO2 std dev±King, Andrew LCalculated using CO2SYS
36 Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
37 pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
38 Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
39 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
40 Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
41 Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
42 Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
43 Alkalinity, totalATµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
44 Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
45 Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
270 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 
Species		2 
Treat		3 
pCO2water_SST_wet [ppmv]
(treatment)		4 
µ [1/day]		5 
µ std dev [±]		6 
PP [µg/µg/h]		7 
PP C std dev [±]		8 
C/P		9 
C/P std dev [±]		10 
N/P		11 
N/P std dev [±]		12 
Fe/P		13 
Fe/P std dev [±]		14 
Co/P		15 
Co/P std dev [±]		16 
Zn/P		17 
Zn/P std dev [±]		18 
Cd/P		19 
Cd/P std dev [±]		20 
NUE Fe [mol/mol/day]
(mol C fixed per mol Fe per day)		21 
NUE Fe std dev [±]		22 
NUE Co [mol/mol/day]
(mol C fixed per mol Co per day)		23 
NUE Co std dev [±]		24 
NUE Zn [mol/mol/day]
(mol C fixed per mol Zn per day)		25 
NUE Zn std dev [±]		26 
NUE Cd [mol/mol/day]
(mol C fixed per mol Cd per day)		27 
NUE Cd std dev [±]		28 
Sal		29 
Temp [°C]		30 
pH
(NBS scale, Potentiometric)		31 
pH std dev [±]
(NBS scale, Potentiometric)		32 
DIC [µmol/kg]
(Coulometric titration)		33 
DIC std dev [±]
(Coulometric titration)		34 
pCO2water_SST_wet [ppmv]
(Calculated using CO2SYS)		35 
pCO2 std dev [±]
(Calculated using CO2SYS)		36 
CSC flag
(Calculated using seacarb afte...)		37 
pH
(total scale, Calculated using...)		38 
CO2 [µmol/kg]
(Calculated using seacarb afte...)		39 
pCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)		40 
fCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)		41 
[HCO3]- [µmol/kg]
(Calculated using seacarb afte...)		42 
[CO3]2- [µmol/kg]
(Calculated using seacarb afte...)		43 
AT [µmol/kg]
(Calculated using seacarb afte...)		44 
Omega Arg
(Calculated using seacarb afte...)		45 
Omega Cal
(Calculated using seacarb afte...)
Attheya sp. (phytoplankton)B12_limited2000.150.001.190.1711411.918.32.122.011.30.0020.0000.780.430.0030.001994.3413656.369911617538.25005120696.073025212.10569054.16285888083.9761683696.97703338.360.0319752720815278.2612.00211.71210.831815.62147.422187.902.233.56
Attheya sp. (phytoplankton)B12_limited3700.160.011.240.0711321.517.52.043.13.60.0030.0011.170.560.0070.004469.686978.55295163849.3260762106.185518039.37426805.99132736919.8761109646.43103338.130.0220702038018278.0421.90386.91385.301954.6493.502191.141.422.26
Attheya sp. (phytoplankton)B12_limited6700.160.011.330.071266.718.91.954.12.00.0040.0011.730.430.0090.002373.760620.64565681156.70201880939.574012336.29373931.24012306816.230498709.14893337.910.0222081368032277.8239.10691.99689.112108.0960.802261.070.921.47
Attheya sp. (phytoplankton)B12_replete2000.260.012.110.0215327.221.02.240.612.50.0560.0253.340.800.0210.0141069.3996437.7909866821.1031560188.912012625.59554571.84782552367.3251883363.56103338.380.0219461619510278.2811.20198.57197.741783.17151.612166.762.303.66
Attheya sp. (phytoplankton)B12_replete3700.290.012.480.0714717.820.73.124.311.30.0450.0262.440.820.0230.0161998.8223730.36291357331.44701076551.180019499.63647655.88742575877.5781416471.03603338.150.022020835316278.0620.30359.98358.481904.2895.402146.581.452.30
Attheya sp. (phytoplankton)B12_replete6700.340.022.920.1224934.636.94.014.01.40.0240.0131.841.070.0130.0095492.3926281.34084107791.81402003637.057049591.875126985.77869034403.6206186120.79403337.920.0121971266131277.8338.00672.74669.942097.0961.902252.920.941.49