Citation:

PANGAEA.835431

Name: Combined effects of CO2 and light on large and small isolates of the unicellular N2-fixing cyanobacterium Crocosphaera watsonii from the western tropical Atlantic Ocean
Published: 2013
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Bacteria; Bottles or small containers/Aquaria (<20 L); Crocosphaera watsonii; Cyanobacteria; Growth/Morphology; Laboratory experiment; Laboratory strains; Light; North Pacific; Other metabolic rates; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species

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Abstract:

We examined the combined effects of light and pCO2 on growth, CO2-fixation and N2-fixation rates by strains of the unicellular marine N2-fixing cyanobacterium Crocosphaera watsonii with small (WH0401) and large (WH0402) cells that were isolated from the western tropical Atlantic Ocean. In low-pCO2-acclimated cultures (190 ppm) of WH0401, growth, CO2-fixation and N2-fixation rates were significantly lower than those in cultures acclimated to higher (present-day 385 ppm, or future 750 ppm) pCO2 treatments. Growth rates were not significantly different, however, in low-pCO2-acclimated cultures of WH0402 in comparison with higher pCO2 treatments. Unlike previous reports for C. watsonii (strain WH8501), N2-fixation rates did not increase further in cultures of WH0401 or WH0402 when acclimated to 750 ppm relative to those maintained at present-day pCO2. Both light and pCO2 had a significant negative effect on gross : net N2-fixation rates in WH0402 and trends were similar in WH0401, implying that retention of fixed N was enhanced under elevated light and pCO2. These data, along with previously reported results, suggest that C. watsonii may have wide-ranging, strain-specific responses to changing light and pCO2, emphasizing the need for examining the effects of global change on a range of isolates within this biogeochemically important genus. In general, however, our data suggest that cellular N retention and CO2-fixation rates of C. watsonii may be positively affected by elevated light and pCO2 within the next 100 years, potentially increasing trophic transfer efficiency of C and N and thereby facilitating uptake of atmospheric carbon by the marine biota.

Keyword(s):

Bacteria; Bottles or small containers/Aquaria (<20 L); Crocosphaera watsonii; Cyanobacteria; Growth/Morphology; Laboratory experiment; Laboratory strains; Light; North Pacific; Other metabolic rates; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species

Related to:

Garcia, Nathan S; Fu, Feixue; Breene, Cynthia L; Yu, Elizabeth K; Bernhardt, Peter W; Mulholland, Margaret R; Hutchins, David A (2013): Combined effects of CO2 and light on large and small isolates of the unicellular N2-fixing cyanobacterium Crocosphaera watsonii from the western tropical Atlantic Ocean. European Journal of Phycology, 48(1), 128-139, https://doi.org/10.1080/09670262.2013.773383

Original version:

Hutchins, David A; Fu, Feixue (2013): Deployment: lab Hutchins 07-12 diazotrophs [dataset]. Biological and Chemical Oceanography Data Management Office, https://www.bco-dmo.org/deployment/59043

Further details:

Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0 [webpage]. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-09-01.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Species	Species		Hutchins, David A
2	Strain	Strain		Hutchins, David A
3	Figure	Fig		Hutchins, David A
4	Irradiance	E	µmol/m²/s	Hutchins, David A
5	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	ppmv	Hutchins, David A		treatment
6	Growth rate	µ	1/day	Hutchins, David A
7	Growth rate, standard deviation	µ std dev	±	Hutchins, David A
8	Diameter	Ø	µm	Hutchins, David A		cell
9	Diameter, standard error	Ø std e	±	Hutchins, David A		cell
10	Carbon fixation rate, photosynthetic	C fix	1/h	Hutchins, David A		normalized
11	Photosynthetic carbon fixation rate, standard deviation	C fix std dev	±	Hutchins, David A		normalized
12	Nitrogen fixation rate	N2 fix	1/h	Hutchins, David A		gross, normalized
13	Nitrogen fixation rate, standard deviation	N2 fix std dev	±	Hutchins, David A		gross, normalized
14	Nitrogen fixation rate	N2 fix	1/h	Hutchins, David A	15N2-tracer method	net, normalized
15	Nitrogen fixation rate, standard deviation	N2 fix std dev	±	Hutchins, David A	15N2-tracer method	net, normalized
16	Nitrogen fixation rate, gross/nitrogen fixation rate, netto ratio	Gross N2 fix/net N2 fix		Hutchins, David A
17	Nitrogen fixation rate, gross/nitrogen fixation rate, netto ratio, standard deviation	Gross N2 fix/net N2 fix std dev	±	Hutchins, David A
18	Photosynthetic carbon fixation rate per cell	C fix/cell	fmol/#/h	Hutchins, David A
19	Photosynthetic carbon fixation rate, standard deviation	C fix std dev	±	Hutchins, David A
20	Nitrogen fixation rate, per cell	N2 fix/cell	fmol/#/h	Hutchins, David A		gross
21	Nitrogen fixation rate, standard deviation	N2 fix std dev	±	Hutchins, David A		gross
22	Nitrogen fixation rate, per cell	N2 fix/cell	fmol/#/h	Hutchins, David A	15N2-tracer method	net
23	Nitrogen fixation rate, standard deviation	N2 fix std dev	±	Hutchins, David A	15N2-tracer method	net
24	Nitrogen, particulate, accumulation, per cell	PN acc/cell	fmol/#/h	Hutchins, David A
25	Nitrogen, particulate, accumulation, standard deviation	PN acc std dev	±	Hutchins, David A
26	Nitrogen fixation rate, gross/particulate nitrogen, netto, accumulation rate ratio	Gross N2 fix/net PN acc		Hutchins, David A
27	Nitrogen fixation rate, gross/particulate nitrogen, netto, accumulation rate ratio, standard deviation	Gross N2 fix/net PN acc std dev	±	Hutchins, David A
28	Salinity	Sal		Hutchins, David A
29	Temperature, water	Temp	°C	Hutchins, David A
30	pH	pH		Hutchins, David A	Potentiometric	NBS scale
31	pH, standard deviation	pH std dev	±	Hutchins, David A	Potentiometric	NBS scale
32	Carbon, inorganic, dissolved	DIC	µmol/kg	Hutchins, David A	Coulometric titration
33	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Hutchins, David A	Coulometric titration
34	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	ppmv	Hutchins, David A	Calculated using CO2SYS
35	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Hutchins, David A	Calculated using CO2SYS
36	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
37	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
38	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
39	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
40	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
41	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
43	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
44	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
45	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1043 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Species	2 Strain	3 Fig	4 E [µmol/m²/s]	5 pCO2water_SST_wet [ppmv] (treatment)	6 µ [1/day]	7 µ std dev [±]	8 Ø [µm] (cell)	9 Ø std e [±] (cell)	10 C fix [1/h] (normalized)	11 C fix std dev [±] (normalized)	12 N2 fix [1/h] (gross, normalized)	13 N2 fix std dev [±] (gross, normalized)	14 N2 fix [1/h] (net, normalized, 15N2-tracer ...)	15 N2 fix std dev [±] (net, normalized, 15N2-tracer ...)	16 Gross N2 fix/net N2 fix	17 Gross N2 fix/net N2 fix std dev [±]	18 C fix/cell [fmol/#/h]	19 C fix std dev [±]	20 N2 fix/cell [fmol/#/h] (gross)	21 N2 fix std dev [±] (gross)	22 N2 fix/cell [fmol/#/h] (net, 15N2-tracer method)	23 N2 fix std dev [±] (net, 15N2-tracer method)	24 PN acc/cell [fmol/#/h]	25 PN acc std dev [±]	26 Gross N2 fix/net PN acc	27 Gross N2 fix/net PN acc std dev [±]	28 Sal	29 Temp [°C]	30 pH (NBS scale, Potentiometric)	31 pH std dev [±] (NBS scale, Potentiometric)	32 DIC [µmol/kg] (Coulometric titration)	33 DIC std dev [±] (Coulometric titration)	34 pCO2water_SST_wet [ppmv] (Calculated using CO2SYS)	35 pCO2 std dev [±] (Calculated using CO2SYS)	36 CSC flag (Calculated using seacarb afte...)	37 pH (total scale, Calculated using...)	38 CO2 [µmol/kg] (Calculated using seacarb afte...)	39 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	40 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	41 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	42 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	43 AT [µmol/kg] (Calculated using seacarb afte...)	44 Omega Arg (Calculated using seacarb afte...)	45 Omega Cal (Calculated using seacarb afte...)
Crocosphaera watsonii (prokaryote)	WH0401	1	300		0.5763	0.01450	2.7415	0.0936	0.016500	0.0005830	0.04000	0.0028200	0.01690000	0.00134000	2.3955	0.2554
Crocosphaera watsonii (prokaryote)	WH0401	1	180		0.5210	0.02840	2.6585	0.0608	0.011900	0.0013300	0.03050	0.0013800	0.00838000	0.00043100	3.6427	0.0750
Crocosphaera watsonii (prokaryote)	WH0401	1	100		0.4661	0.00398	2.5902	0.0883	0.008920	0.0007530	0.03000	0.0046700	0.00415000	0.00058300	7.2115	0.1211
Crocosphaera watsonii (prokaryote)	WH0401	1	50		0.2719	0.01360	2.2829	0.0723	0.003850	0.0001890	0.02220	0.0005110	0.00270000	0.00051000	8.7453	1.3970
Crocosphaera watsonii (prokaryote)	WH0401	1	25		0.1583	0.01040	2.2800		0.000803	0.0000419	0.00973	0.0004640	0.00000884	0.00000413
Crocosphaera watsonii (prokaryote)	WH0402	1	300		0.8141	0.03790	5.5122	0.1485	0.024200	0.0018700	0.04510	0.0027100	0.01610000	0.00059000	2.8096	0.1648
Crocosphaera watsonii (prokaryote)	WH0402	1	180		0.7663	0.02310	5.3902	0.1299	0.023200	0.0006660	0.04450	0.0028100	0.01520000	0.00041100	2.9148	0.1292
Crocosphaera watsonii (prokaryote)	WH0402	1	100		0.5879	0.01370	4.8537	0.0968	0.015000	0.0034400	0.03980	0.0052700	0.00850000	0.00219000	5.1152	0.8803
Crocosphaera watsonii (prokaryote)	WH0402	1	50		0.4391	0.03690	4.6707	0.0669	0.009120	0.0009660	0.02770	0.0019100	0.00503000	0.00028600	5.5532	0.5758
Crocosphaera watsonii (prokaryote)	WH0402	1	25		0.1931	0.00301	4.6098	0.0935	0.003160	0.0003970	0.01000	0.0000861	0.00065600	0.00008110	15.7753	2.1655
Crocosphaera watsonii (prokaryote)	WH0003	1	300		0.6351	0.01010	5.7317	0.1932
Crocosphaera watsonii (prokaryote)	WH0003	1	180		0.5525	0.00414	5.5122	0.1452
Crocosphaera watsonii (prokaryote)	WH0003	1	100		0.4633	0.00768	5.4146	0.1658
Crocosphaera watsonii (prokaryote)	WH0003	1	40		0.2236	0.00388	4.9024	0.0438
Crocosphaera watsonii (prokaryote)	WH0003	1	18		0.0762		4.8780	0.0891
Crocosphaera watsonii (prokaryote)	WH0401	2		190	0.0866	0.03290									3.4140	0.1646											33.5	28	8.43	0.03
Crocosphaera watsonii (prokaryote)	WH0401	2		386	0.3154	0.05100									2.6350	0.5481											33.5	28	8.19	0.00
Crocosphaera watsonii (prokaryote)	WH0401	2		750	0.4000	0.01170									1.9700	0.2097											33.5	28	8.01	0.01
Crocosphaera watsonii (prokaryote)	WH0402	2		190	0.5623	0.03380									2.9513	0.1147											33.5	28	8.40	0.01
Crocosphaera watsonii (prokaryote)	WH0402	2		386	0.6253	0.05970									2.5114	0.1719											33.5	28	8.14	0.01
Crocosphaera watsonii (prokaryote)	WH0402	2		750	0.6500	0.04360									2.1549	0.1999											33.5	28	8.01	0.01
Crocosphaera watsonii (prokaryote)	WH0401	3, 4, 5	300	761	0.5427	0.04360					0.05340	0.0013600	0.00800000	0.00125000	6.3782	0.9704	10.7423	0.3700	2.2814	0.1818	0.3479	0.0745	1.4286	0.0981	1.5949	0.0405	33.5	28	8.02	0.01	2128	19	674	24	27	7.87	17.43	657.94	655.94	1941.06	169.50	2352.33	2.76	4.15
Crocosphaera watsonii (prokaryote)	WH0401	3, 4, 5	180	761	0.4539	0.03930					0.05230	0.0026000	0.00703000	0.00081300	6.6361	0.9123	9.5543	0.4168	2.2895	0.0753	0.3076	0.0308	1.2886	0.1058	1.7911	0.0889	33.5	28	8.02	0.01	2128	19	674	24	27	7.87	17.43	657.94	655.94	1941.06	169.50	2352.33	2.76	4.15
Crocosphaera watsonii (prokaryote)	WH0401	3, 4, 5	100	761	0.3435	0.01080					0.05130	0.0015100	0.00512000	0.00031200	10.4948	0.8133	7.0618	0.2124	2.0571	0.1154	0.2088	0.0096	0.9159	0.0255	2.2428	0.0715	33.5	28	8.02	0.01	2128	19	674	24	27	7.87	17.43	657.94	655.94	1941.06	169.50	2352.33	2.76	4.15
Crocosphaera watsonii (prokaryote)	WH0401	3, 4, 5	300	386	0.5891	0.01960					0.05600	0.0089600	0.00805000	0.00165000	6.2822	0.2609	10.3489	0.5941	2.0884	0.1761	0.2979	0.0355	1.3621	0.1088	1.5718	0.2515	33.5	28	8.21	0.01	2017	35	394	16	27	8.06	10.25	386.87	385.70	1767.68	239.07	2349.08	3.89	5.86
Crocosphaera watsonii (prokaryote)	WH0401	3, 4, 5	180	386	0.5261	0.00808					0.05790	0.0013000	0.00860000	0.00057600	6.0140	0.4478	8.9128	0.2580	2.1503	0.1187	0.3180	0.0170	1.2135	0.0501	1.7703	0.0399	33.5	28	8.21	0.01	2017	35	394	16	27	8.06	10.25	386.87	385.70	1767.68	239.07	2349.08	3.89	5.86
Crocosphaera watsonii (prokaryote)	WH0401	3, 4, 5	100	386	0.2584	0.01280					0.03900	0.0017400	0.00391000	0.00023000	10.9866	0.7227	6.0933	0.4214	2.1817	0.1111	0.2128	0.0213	1.0505	0.0451	2.0793	0.0928	33.5	28	8.21	0.01	2017	35	394	16	27	8.06	10.25	386.87	385.70	1767.68	239.07	2349.08	3.89	5.86
Crocosphaera watsonii (prokaryote)	WH0401	3, 4, 5	300	190	0.2994	0.03130					0.03480	0.0065200	0.00428000	0.00058600	8.1014	0.8385	9.2812	1.0620	1.8322	0.3251	0.2262	0.0298	1.0980	0.0373	1.6811	0.3148	33.5	28	8.45	0.01	1748	23	181	6	27	8.30	4.71	177.77	177.23	1411.54	331.75	2230.05	5.39	8.13
Crocosphaera watsonii (prokaryote)	WH0401	3, 4, 5	180	190	0.2875	0.02250					0.03680	0.0028000	0.00368000	0.00018800	10.1049	0.4153	8.2820	0.6203	2.0705	0.2713	0.2055	0.0213	1.1058	0.0701	2.0000	0.1461	33.5	28	8.45	0.01	1748	23	181	6	27	8.30	4.71	177.77	177.23	1411.54	331.75	2230.05	5.39	8.13
Crocosphaera watsonii (prokaryote)	WH0401	3, 4, 5	100	190	0.1437	0.05070					0.02110	0.0010500	0.00236000	0.00021700	9.8853	0.5048	5.7571	0.3639	1.2690	0.0990	0.1420	0.0155	0.8523	0.0243	1.4847	0.0740	33.5	28	8.45	0.01	1748	23	181	6	27	8.30	4.71	177.77	177.23	1411.54	331.75	2230.05	5.39	8.13
Crocosphaera watsonii (prokaryote)	WH0402	3, 4, 5	300	750	0.5827	0.02270					0.03360	0.0023500	0.01020000	0.00016500	3.4193	0.3616	63.9338	6.0202	8.5404	0.6496	2.2816	0.0585	8.2200	0.8026	0.9671	0.0677	33.5	28	8.05	0.01	2150	18	628	31	27	7.90	16.35	617.21	615.34	1951.09	182.56	2392.79	2.97	4.47
Crocosphaera watsonii (prokaryote)	WH0402	3, 4, 5	180	750	0.5770	0.05790					0.03230	0.0009240	0.00966000	0.00095200	3.4397	0.4420	47.2081	1.1589	7.5704	0.0787	1.8186	0.0618	7.0925	0.3718	0.9671	0.0276	33.5	28	8.05	0.01	2150	18	628	31	27	7.90	16.35	617.21	615.34	1951.09	182.56	2392.79	2.97	4.47
Crocosphaera watsonii (prokaryote)	WH0402	3, 4, 5	100	750	0.4796	0.03560					0.03000	0.0013400	0.00768000	0.00019100	3.9221	0.2568	27.6609	2.3481	6.2780	0.4493	1.2004	0.0418	5.9427	0.2349	1.0566	0.0471	33.5	28	8.05	0.01	2150	18	628	31	27	7.90	16.35	617.21	615.34	1951.09	182.56	2392.79	2.97	4.47
Crocosphaera watsonii (prokaryote)	WH0402	3, 4, 5	50	750	0.2959	0.01470					0.02730	0.0039900	0.00445000	0.00031200	6.1512	0.7880	14.3539	0.8298	4.6145	0.2892	0.8336	0.0378	2.4751	0.3575	1.6916	0.2470	33.5	28	8.05	0.01	2150	18	628	31	27	7.90	16.35	617.21	615.34	1951.09	182.56	2392.79	2.97	4.47
Crocosphaera watsonii (prokaryote)	WH0402	3, 4, 5	18	750	0.0565	0.00582					0.00657	0.0008770			5.4614	0.4996	9.0416	0.8532	1.7415	0.2432	0.2167	0.0207	1.8065	0.1476	0.9882	0.1320	33.5	28	8.05	0.01	2150	18	628	31	27	7.90	16.35	617.21	615.34	1951.09	182.56	2392.79	2.97	4.47
Crocosphaera watsonii (prokaryote)	WH0402	3, 4, 5	300	386	0.6652	0.02720					0.03550	0.0017700	0.00992000	0.00089500	3.7569	0.3497	80.6415	3.3612	7.8413	0.2781	2.3594	0.3126	7.5952	0.5098	1.1263	0.0560	33.5	28	8.22	0.01	2061	12	395	14	27	8.07	10.21	385.30	384.13	1801.48	249.31	2405.24	4.05	6.11
Crocosphaera watsonii (prokaryote)	WH0402	3, 4, 5	180	386	0.6305	0.01900					0.03320	0.0004510	0.00830000	0.00045000	4.1693	0.0953	55.9421	3.4794	6.8784	0.5487	2.0624	0.3089	7.1400	0.0944	1.0605	0.0144	33.5	28	8.22	0.01	2061	12	395	14	27	8.07	10.21	385.30	384.13	1801.48	249.31	2405.24	4.05	6.11
Crocosphaera watsonii (prokaryote)	WH0402	3, 4, 5	100	386	0.5062	0.03350					0.03040	0.0041100	0.00608000	0.00085900	5.2312	0.3388	36.2452	0.7303	6.2620	0.1947	1.6053	0.0729	5.7420	0.4663	1.1173	0.1512	33.5	28	8.22	0.01	2061	12	395	14	27	8.07	10.21	385.30	384.13	1801.48	249.31	2405.24	4.05	6.11
Crocosphaera watsonii (prokaryote)	WH0402	3, 4, 5	50	386	0.2563	0.02510					0.02470	0.0036600	0.00503000	0.00111000	5.5309	0.1973	13.5240	0.8226	4.0287	0.2435	0.6777	0.1013	3.5263	0.3635	1.3497	0.1998	33.5	28	8.22	0.01	2061	12	395	14	27	8.07	10.21	385.30	384.13	1801.48	249.31	2405.24	4.05	6.11
Crocosphaera watsonii (prokaryote)	WH0402	3, 4, 5	18	386	0.0977	0.01220					0.00329	0.0007490			7.9683	0.4326	6.8955	1.1068	1.7463	0.0755	0.3224	0.0147	2.1581	0.1854	0.8383	0.1908	33.5	28	8.22	0.01	2061	12	395	14	27	8.07	10.21	385.30	384.13	1801.48	249.31	2405.24	4.05	6.11