Schmithüsen, Tosia; Niehoff, Barbara; Auel, Holger (2014): Protein content of Calanus finmarchicus from the North Atlantic collected during Maria S. Merian cruise MSM26 in spring 2013 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.835180, In: Schmithüsen, T et al. (2014): Enzyme activities of digestive and metabolic enzymes of Calanus finmarchicus from Maria S. Merian cruise MSM26 in spring 2013 [dataset publication series]. PANGAEA, https://doi.org/10.1594/PANGAEA.835181
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Abstract:
For the determination of water-soluble protein content of C. finmarchicus of the different stations the Qubit® Protein Assay Kit (Invitrogen) was used. Analysis was performed with extracts of 10 copepods. Working solution was prepared with Qubit® protein reagent and Qubit® protein buffer (1:200). 190 µL working solution was pipetted into each well of a micro plate and 10 µL of sample or Qubit® protein standard (0, 200 and 400 ng/µL) was added. Solutions were mixed and incubated for 15 min at room temperature. Measurements were conducted with a micro plate reader (TriStar LB 941, Berthold Technologies) at 485 nm excitation and 590 nm emission, using the software MikroWin2000 (Berthold Technologies).
Related to:
Schmithüsen, Tosia (2013): Enzyme activities in Calanus finmarchicus across the North Atlantic - a comparison of populations from different longitudes. Master Thesis, University of Bremen, Alfred Wegener Institut für Polar und Meeresforschung, Bremerhaven, hdl:10013/epic.43161
Project(s):
Basin Scale Analysis, Synthesis and Integration (EURO-BASIN)
Funding:
Seventh Framework Programme (FP7), grant/award no. 264933: Basin Scale Analysis, Synthesis and Integration
Coverage:
Median Latitude: 60.579143 * Median Longitude: -32.364357 * South-bound Latitude: 56.446000 * West-bound Longitude: -56.074000 * North-bound Latitude: 62.819000 * East-bound Longitude: -10.869000
Date/Time Start: 2013-03-25T13:24:00 * Date/Time End: 2013-04-11T10:43:00
Minimum Elevation: -3631.0 m * Maximum Elevation: -1144.0 m
Event(s):
MSM26_126-9 * Latitude Start: 61.498000 * Longitude Start: -10.996000 * Latitude End: 61.445000 * Longitude End: -10.869000 * Date/Time Start: 2013-03-25T13:24:00 * Date/Time End: 2013-03-25T16:27:00 * Elevation Start: -1321.0 m * Elevation End: -1217.0 m * Location: North Atlantic * Campaign: MSM26 * Basis: Maria S. Merian * Method/Device: Double opening/closing plankton net (D-MOC) * Comment: rope length max. 1855m /EL II
MSM26_127-17 * Latitude Start: 62.819000 * Longitude Start: -21.360000 * Latitude End: 62.738000 * Longitude End: -21.294000 * Date/Time Start: 2013-03-28T12:53:00 * Date/Time End: 2013-03-28T15:34:00 * Elevation Start: -1144.0 m * Elevation End: -1175.0 m * Location: North Atlantic * Campaign: MSM26 * Basis: Maria S. Merian * Method/Device: Double opening/closing plankton net (D-MOC) * Comment: rope length max. 2006m /EL II
MSM26_131-17 * Latitude Start: 60.467000 * Longitude Start: -23.530000 * Latitude End: 60.431000 * Longitude End: -23.354000 * Date/Time Start: 2013-03-31T08:29:00 * Date/Time End: 2013-03-31T11:33:00 * Elevation Start: -2069.0 m * Elevation End: -2075.0 m * Location: North Atlantic * Campaign: MSM26 * Basis: Maria S. Merian * Method/Device: Double opening/closing plankton net (D-MOC) * Comment: rope length max. 2351m /EL2
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | ||||
| 2 | Latitude of event | Latitude | ||||
| 3 | Longitude of event | Longitude | ||||
| 4 | Date/Time of event | Date/Time | ||||
| 5 | Sample ID | Sample ID | Schmithüsen, Tosia | |||
| 6 | Taxon/taxa | Taxa | Schmithüsen, Tosia | |||
| 7 | Uniform resource locator/link to reference | URL ref | Schmithüsen, Tosia | WoRMS Aphia ID | ||
| 8 | Life stage | Life stage | Schmithüsen, Tosia | |||
| 9 | Number of individuals | Ind No | # | Schmithüsen, Tosia | pooledfor measurements | |
| 10 | Proteins per individual | Prot/ind | µg/# | Schmithüsen, Tosia | TriStar LB 941 Microplate Reader (Berthold Technologies) |
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Size:
126 data points