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Gazeau, Frédéric; Guieu, Cecile; Taillandier, V; Alliouane, Samir; Giani, Michele; Sallon, Amèlie; Pedrotti, Maria Luiza; Pitta, Paraskevi; Celussi, Mauro; Giannakourou, Antonia; Maugendre, Laure; Louis, Justine (2017): Villefranche MedSeA mesocosm experiment 2013: perturbation studies [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.835117, In: Gazeau, F et al. (2013): Villefranche MedSeA mesocosm experiment 2013: perturbation studies and field studies [dataset publication series]. PANGAEA, https://doi.org/10.1594/PANGAEA.835119

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Keyword(s):
Calcification/Dissolution; Coast and continental shelf; Community composition and diversity; Entire community; Field experiment; Mediterranean Sea; Mesocosm or benthocosm; Other metabolic rates; Pelagos; Primary production/Photosynthesis; Respiration; Temperate
Related to:
Maugendre, Laure; Gattuso, Jean-Pierre; Poulton, Alex J; Dellisanti, Walter; Gaubert, M; Guieu, Cecile; Gazeau, Frédéric (2015): No detectable effect of ocean acidification on plankton metabolism in the NW oligotrophic Mediterranean Sea: results from two mesocosm studies. Estuarine, Coastal and Shelf Science, https://doi.org/10.1016/j.ecss.2015.03.009
Further details:
Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb
Funding:
Seventh Framework Programme (FP7), grant/award no. 265103: Mediterranean Sea Acidification in a Changing Climate
Coverage:
Latitude: 43.697000 * Longitude: 7.312000
Date/Time Start: 2013-02-17T08:00:00 * Date/Time End: 2013-03-05T13:47:00
Minimum DEPTH, water, experiment: 0.50 m * Maximum DEPTH, water, experiment: 12.50 m
Event(s):
Villefranche-2013-CTD-perturbation * Latitude: 43.697000 * Longitude: 7.312000 * Date/Time Start: 2013-02-17T07:30:00 * Date/Time End: 2013-03-05T14:00:00 * Location: Bay of Villefranche, Mediterranean Sea * Method/Device: Mesocosm experiment (MESO)
Villefranche-2013-IWS-perturbation * Latitude: 43.697000 * Longitude: 7.312000 * Date/Time Start: 2013-02-17T07:30:00 * Date/Time End: 2013-03-05T14:00:00 * Location: Bay of Villefranche, Mediterranean Sea * Method/Device: Mesocosm experiment (MESO)
Comment:
<0= below detection limits. In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-08-21.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Event labelEvent
2Other eventOther eventGazeau, Frédéric
3TreatmentTreatGazeau, Frédéric
4Day of experimentDOEdayGuieu, Cecile
5DATE/TIMEDate/TimeGeocode
6DEPTH, water, experimentDepth water expmGeocode
7Temperature, waterTemp°CTaillandier, VCTD
8SalinitySalTaillandier, VCTD
9Density, sigma, in situSigma in situkg/m3Taillandier, VCTD
10FluorometerFluorometerVTaillandier, VCTD
11OxygenO2µmol/lTaillandier, VCTD with attached oxygen sensor
12IrradianceEµmol/m2/sTaillandier, VCTD
13pHpHTaillandier, VCTDTotal scale; based on a empiric relationship between pH calculated from TA and TCO2 and integrated pH from the CTD (originally on the NBS)
14Carbonate system computation flagCSC flagAlliouane, SamirCalculated using seacarb
15pHpHAlliouane, SamirCalculated using seacarbTotal scale
16Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmAlliouane, SamirCalculated using seacarb
17Bicarbonate ion[HCO3]-µmol/kgAlliouane, SamirCalculated using seacarb
18Carbonate ion[CO3]2-µmol/kgAlliouane, SamirCalculated using seacarb
19Carbon, inorganic, dissolvedDICµmol/kgAlliouane, SamirAIRICA analyzer (Miranda)
20Alkalinity, totalATµmol/kgAlliouane, SamirTitration potentiometric, Metrohm
21Aragonite saturation stateOmega ArgAlliouane, SamirCalculated using seacarb
22Calcite saturation stateOmega CalAlliouane, SamirCalculated using seacarb
23Ammonium[NH4]+µmol/lSallon, AmèlieSkalar AutoAnalyser
24SiliconSiµmol/lGiani, MicheleSkalar AutoAnalyser
25Nitrogen, total, particulateTPNµmol/lGiani, MicheleElement analyser CHN, Costech
26Carbon, total, particulateTPCµmol/lGiani, MicheleElement analyser CHN, Costech
27Carbon, organic, particulatePOCµmol/lGiani, MicheleElement analyser CHN, Costech
28Phosphorus, particulatePPµmol/lGiani, MicheleColorimetric, GF/F filters, autoanalyzer (Bran Luebbe)Total
29Chlorophyll c3Chl c3µg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
30Chlorophyll c1+c2Chl c1+c2µg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
31Chlorophyllide aChlide aµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)Sum
32PeridininPeridµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
33Phaeophorbide aPhaeopho aµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
3419-ButanoyloxyfucoxanthinBut-fucoµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
35FucoxanthinFucoµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
36NeoxanthinNeoµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
37PrasinoxanthinPrasµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
38ViolaxanthinViolaµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
3919-HexanoyloxyfucoxanthinHex-fucoµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
40DiadinoxanthinDiadinoµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
41AlloxanthinAlloµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
42DiatoxanthinDiatoµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
43ZeaxanthinZeaµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
44LuteinLutµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
45Bacteriochlorophyll aBChl aµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
46Divinyl chlorophyll bDV chl bµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
47Chlorophyll bChl bµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
48Divinyl chlorophyll aDV chl aµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
49Chlorophyll aChl aµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)
50Chlorophyll aChl aµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)Total
51Phaeophytin aPhaeophytin aµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)Sum
52CaroteneCaroteneµg/lSallon, AmèlieHigh Performance Liquid Chromatography (HPLC)Sum
53ProchlorococcusProchlorococcus#/mlPedrotti, Maria LuizaFlow cytometry
54SynechococcusSynechococcus#/mlPedrotti, Maria LuizaFlow cytometry
55Picoeukaryotes, autotrophicPEuk auto#/mlPedrotti, Maria LuizaFlow cytometry
56NanoeukaryotesNEuk#/mlPedrotti, Maria LuizaFlow cytometry
57BacteriaBact#/mlPitta, ParaskeviFlow cytometry
58Bacteria, low DNA fluorescenceBact LDNA#/mlPitta, ParaskeviFlow cytometry
59Bacteria, high DNA fluorescenceBact HDNA#/mlPitta, ParaskeviFlow cytometry
60Viral abundanceVirus#/mlPitta, ParaskeviFlow cytometry
61Virus, low DNA fluorescenceVirus LDNA#/mlPitta, ParaskeviFlow cytometry
62Virus, medium DNA fluorescenceVirus HDNA#/mlPitta, ParaskeviFlow cytometry
63Virus, high DNA fluorescenceVirus HDNA#/mlPitta, ParaskeviFlow cytometry
64beta-glucosidase activityMUF-bgluµmol/l/hCelussi, MauroMUF-beta-D-glucose fluorogenic substrate
65Lipase activityLipase activitynmol/l/hCelussi, MauroMUF-beta-D-glucose fluorogenic substrate
66Chitinase activityChitinase activitynmol/l/hCelussi, MauroMUF-beta-D-glucose fluorogenic substrate
67Phosphatase activityMUF-Phosµmol/l/hCelussi, MauroMUF-beta-D-glucose fluorogenic substrate
68Leucine aminopeptidase activityLeu aminopepnmol/l/hCelussi, MauroMCA-leucine fluorogenic substrate
69ProkaryotesProkaryotes#/mlCelussi, MauroEpifluorescence microscopyHighly active
70ProkaryotesProkaryotes#/mlCelussi, MauroEpifluorescence microscopyTotal
71Net community production of oxygenNCP O2µmol/l/dayGazeau, FrédéricIncubation (INCUB)Incubated in situ at 4m depth in 5 x biological oxygen demand (BOD) 60ml bottles over 24h. Rate estimated using a linear regression between initial and final oxygen concentration
72Net community production of oxygen, standard errorNCP O2 std e±Gazeau, FrédéricCalculated from linear regression
73Respiration rate, oxygenResp O2µmol/l/dayGazeau, FrédéricIncubation (INCUB)Community; Incubated in the laboratory in the dark at in situ temperature in 5 x biological oxygen demand (BOD) 60ml bottles over 24h. Rate estimated using a linear regression between initial and final oxygen concentration
74Respiration rate, oxygen, standard deviationResp O2 std dev±Gazeau, FrédéricCalculated from linear regressionCommunity
75Gross community production of oxygenGCP O2µmol/l/dayGazeau, FrédéricCalculatedCalculated as the difference between net community production and respiration
76Gross community production of oxygen, standard errorGCP O2 std e±Gazeau, FrédéricCalculated using error propagation
77Bacterial productionBPng/l/hGiannakourou, AntoniaIncubation (INCUB)3H-Leucine incorporation in triplicated bottles incubated in the laboratory in the dark at in situ temperature over 2h
78Primary production of POC as determined by 14C POC productionPP 14C POCµmol/l/dayMaugendre, Laure
79Primary production of POC as determined by 14C POC production, standard deviationPP 14C POC std dev±Maugendre, Laure
80Calcification rate of calcium carbonateCalc rate CaCO3µmol/l/dayMaugendre, Laure
81Calcification rate, standard deviationCalc rate std dev±Maugendre, Laure
82Exudation as determined by 14C DOC productionExud 14C DOCµmol/l/dayMaugendre, Laure
83Exudation as determined by 14C DOC production, standard deviationExud 14C DOC std dev±Maugendre, Laure
84Transparent exopolymer particles as Gum Xanthan equivalents per volumeTEPµg Xeq/lPedrotti, Maria Luiza
85Nitrate[NO3]-µmol/lLouis, JustineSpectrophotometric method with Liquid Waveguide Capillary Cell (LWCC)Filtration onto 0.2 µm polycarbonate filters
86Phosphate[PO4]3-µmol/lLouis, JustineSpectrophotometric method with Liquid Waveguide Capillary Cell (LWCC)Filtration onto 0.2 µm polycarbonate filters
87Iron, dissolvedFe dissnmol/lLouis, JustineFIA with chemiluminescence detectionFiltration onto 0.2 µm polycarbonate filters
88Nitrogen, organic, dissolvedDONµmol/lLouis, JustineUV light photo oxidation then spectrophotometric method with LWCCCalculated as the difference between total dissolved nitrogen measured (TDN) and nitrate concentration
89Phosphate, organic, dissolvedDOPµmol/lLouis, JustineUV light photo oxidation then spectrophotometric method with LWCCCalculated as the difference between total dissolved phosphorus measured (TDP) and phosphate concentration
90Validation flag/commentFlag commYang, YanCalculated using seacarb after Nisumaa et al. (2010)
91pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)Total scale
92Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
93Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
94Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
95Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
96Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
97Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
98Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
67487 data points

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