Lin, Yu-Shih; Lipp, Julius S; Elvert, Marcus; Holler, Thomas; Hinrichs, Kai-Uwe (2014): Biphytane and stable carbon isotopic values of sugars and glycerol from ODP Hole 204-1245D and 201-1229A [dataset publication series]. PANGAEA, https://doi.org/10.1594/PANGAEA.834735
Always quote citation above when using data! You can download the citation in several formats below.
Published: 2014-08-07 • DOI registered: 2014-09-04
Abstract:
The membrane lipids diglycosyl-glycerol dibiphytanyl glycerol tetraethers (2G-GDGTs) in marine subsurface sediments are believed to originate from uncultivated benthic archaea, yet the production of 2G-GDGTs from subseafloor samples has not been demonstrated in vitro. In order to validate sedimentary biosynthesis of 2G-GDGTs, we performed a stable carbon isotope probing experiment on a subseafloor sample with six different 13C-labelled substrates (bicarbonate, methane, acetate, leucine, glucose and Spirulina platensis biomass). After 468 days of anoxic incubation, only glucose and S. platensis resulted in label uptake in lipid moieties of 2G-GDGTs, indicating incorporation of carbon from these organic substrates. The hydrophobic moieties of 2G-GDGTs showed minimal label incorporation, with up to 4 per mil 13C enrichment detected in crenarchaeol-derived tricyclic biphytane from the S. platensis-supplemented slurries. The 2G-GDGT-derived glucose or glycerol moieties also showed 13C incorporation (Dd13C = 18 - 38 per mil) in the incubations with glucose or S. platensis, consistent with a lipid salvage mechanism utilized by marine benthic archaea to produce new 2G-GDGTs. The production rates were nevertheless rather slow, even when labile organic matter was supplied. The 2G-GDGT turnover times of 1700 - 20 500 years were much longer than those estimated for subseafloor microbial communities, implying that sedimentary 2G-GDGTs as biomarkers of benthic archaea are cumulative records of past and present generations.
Related to:
Lin, Yu-Shih; Lipp, Julius S; Elvert, Marcus; Holler, Thomas; Hinrichs, Kai-Uwe (2013): Assessing production of the ubiquitous archaeal diglycosyl tetraether lipids in marine subsurface sediment using intramolecular stable isotope probing. Environmental Microbiology, 15(5), 1634-1646, https://doi.org/10.1111/j.1462-2920.2012.02888.x
Project(s):
Ocean Drilling Program (ODP)
Coverage:
Median Latitude: 26.065367 * Median Longitude: -109.418450 * South-bound Latitude: -10.976200 * West-bound Longitude: -125.148850 * North-bound Latitude: 44.586150 * East-bound Longitude: -77.957650
Date/Time Start: 2002-03-06T13:30:00 * Date/Time End: 2002-08-11T04:00:00
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Size:
3 datasets
Download Data
Datasets listed in this publication series
- Lin, Y-S; Lipp, JS; Elvert, M et al. (2014): (Table S2) Stable carbon isotopic values of sugars and glycerol of the 2G-GDGT fraction purified from ODP Hole 201-1229A. https://doi.org/10.1594/PANGAEA.834734
- Lin, Y-S; Lipp, JS; Elvert, M et al. (2014): (Fig. 3 & TabS1) Stable carbon isotope compositions of biphytanes, mannose and galactose as well as glucose and glycerol from ODP Hole 204-1245D. https://doi.org/10.1594/PANGAEA.834732
- Lin, Y-S; Lipp, JS; Elvert, M et al. (2014): (Fig. 1) Bacterial intact polar lipids and Archaeal intact polar lipids in the time zero sample and incubationsis from ODP Hole 204-1245D. https://doi.org/10.1594/PANGAEA.834731