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Hamilton, Trevor James; Holcombe, Adam; Tresguerres, Martin (2013): CO2-induced ocean acidification increases anxiety in Rockfish via alteration of GABAA receptor functioning [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.834309, Supplement to: Hamilton, TJ et al. (2013): CO2-induced ocean acidification increases anxiety in Rockfish via alteration of GABAA receptor functioning. Proceedings of the Royal Society B-Biological Sciences, 281(1775), 20132509, https://doi.org/10.1098/rspb.2013.2509

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Abstract:
The average surface pH of the ocean is dropping at a rapid rate due to the dissolution of anthropogenic CO2, raising concerns for marine life. Additionally, some coastal areas periodically experience upwelling of CO2-enriched water with reduced pH. Previous research has demonstrated ocean acidification (OA)-induced changes in behavioural and sensory systems including olfaction, which is due to altered function of neural gamma-aminobutyric acid type A (GABAA) receptors. Here, we used a camera-based tracking software system to examine whether OA-dependent changes in GABAA receptors affect anxiety in juvenile Californian rockfish (Sebastes diploproa). Anxiety was estimated using behavioural tests that measure light/dark preference (scototaxis) and proximity to an object. After one week in OA conditions projected for the next century in the California shore (1125 ± 100 µatm, pH 7.75), anxiety was significantly increased relative to controls (483 ± 40 µatm CO2, pH 8.1). The GABAA-receptor agonist muscimol, but not the antagonist gabazine, caused a significant increase in anxiety consistent with altered Cl- flux in OA-exposed fish. OA-exposed fish remained more anxious even after 7 days back in control seawater; however, they resumed their normal behaviour by day 12. These results show that OA could severely alter rockfish behaviour; however, this effect is reversible.
Keyword(s):
Animalia; Behaviour; Chordata; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Laboratory experiment; Nekton; North Pacific; Pelagos; Sebastes diploproa; Single species; Temperate
Further details:
Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0 [webpage]. https://cran.r-project.org/package=seacarb
Project(s):
Ocean Acidification International Coordination Centre (OA-ICC)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-07-25.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1SpeciesSpeciesHamilton, Trevor James
2FigureFigHamilton, Trevor James
3TableTabHamilton, Trevor James
4TreatmentTreatHamilton, Trevor James
5Time in secondsTimesHamilton, Trevor Jamesin dark zone
6Time, standard errorTime std e±Hamilton, Trevor Jamesin dark zone
7Time in secondsTimesHamilton, Trevor Jamesnear object
8Time, standard errorTime std e±Hamilton, Trevor Jamesnear object
9DistanceDistancemHamilton, Trevor Jamestravelled
10Distance, standard errorDistance std e±Hamilton, Trevor Jamestravelled
11ImmobilityImmobilsHamilton, Trevor James
12Immobility, standard errorImmobil std e±Hamilton, Trevor James
13SalinitySalHamilton, Trevor James
14Salinity, standard errorSal std e±Hamilton, Trevor James
15Temperature, waterTemp°CHamilton, Trevor James
16Temperature, water, standard errorT std e±Hamilton, Trevor James
17pHpHHamilton, Trevor JamesPotentiometricNBS scale
18pH, standard errorpH std e±Hamilton, Trevor JamesPotentiometricNBS scale
19Alkalinity, totalATµmol/kgHamilton, Trevor James
20Alkalinity, total, standard errorAT std e±Hamilton, Trevor James
21Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmHamilton, Trevor JamesCalculated using CO2SYS
22Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard errorpCO2water_SST_wet std e±Hamilton, Trevor JamesCalculated using CO2SYS
23Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
24pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
25Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
26Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
27Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
28Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
29Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
30Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
31Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
32Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
466 data points

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